The milk fat globule membrane(MFGM)is a complex structure with numerous functions,and its composition is affected by many factors.There have been few systematic investigations on goat MFGM proteome profiling during la...The milk fat globule membrane(MFGM)is a complex structure with numerous functions,and its composition is affected by many factors.There have been few systematic investigations on goat MFGM proteome profiling during lactation.Individual milk samples from 15 healthy dairy goats were obtained at six lactation time points for investigation of the MFGM proteome using both data-independent acquisition(DIA)and data-dependent acquisition(DDA)proteomics techniques combined with multivariate statistical analysis.Using the DIA method,890 variably abundant MFGM proteins were discovered throughout the lactation cycle.From 1 to 240 d,butyrophilin subfamily 1 member A1,lipoprotein lipase,perilipin-2,and adipose triglyceride lipase were upregulated,while APOE,complement C3,clusterin,and IgG were downregulated.Furthermore,from 1 to 90 d,annexin A1,annexin A2,and antithrombin-ll were downregulated,then upregulated by d 240.Albumin had a high degree of connectedness,indicating that it was a key protein,according to protein-protein interaction research.Overall,our findings gave new insights into the biological features of MFGM protein in goat milk throughout lactation,which may aid in the creation of specialized MFGM products and infant formula.展开更多
Proteomics comparison of human milk fat globular membrane proteins collected from Korean and Han ethnic groups in China was performed using data independent acquisition (DIA) technique. Nearly 1596 proteins were ident...Proteomics comparison of human milk fat globular membrane proteins collected from Korean and Han ethnic groups in China was performed using data independent acquisition (DIA) technique. Nearly 1596 proteins were identified and they were mainly distributed in single-organism process/cellular process of biological process (BP), cell/cell part of cellular component (CC), and binding of molecular function (MF) in Gene ontology (GO) annotation and metabolic pathway in Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. A total of 238 differentially expressed proteins (DEPs) including 72 enzymes were detected between the two ethnic groups and 142 were higher in Korean women milk. Enrichment analysis showed that 374 GO functions and 118 pathways where DEPs participated were significantly enriched (q < 0.05). Protein-protein interaction (PPI) network denoted that the DEP of actin, cytoplasmic 1 interacted most with others. Data unraveled variations in proteins between different ethnic human milks and provided useful information for development of infant formula products.展开更多
基金This work was supportedby theNational KeyR&D Program of China(2022YFD1301005)the Shandong Provincial Natural Science Foundation,China(ZR2022MC184)the High-level Talents Foundation of Qingdao Agricultural University,China(665/1120053,665/1120080).
文摘The milk fat globule membrane(MFGM)is a complex structure with numerous functions,and its composition is affected by many factors.There have been few systematic investigations on goat MFGM proteome profiling during lactation.Individual milk samples from 15 healthy dairy goats were obtained at six lactation time points for investigation of the MFGM proteome using both data-independent acquisition(DIA)and data-dependent acquisition(DDA)proteomics techniques combined with multivariate statistical analysis.Using the DIA method,890 variably abundant MFGM proteins were discovered throughout the lactation cycle.From 1 to 240 d,butyrophilin subfamily 1 member A1,lipoprotein lipase,perilipin-2,and adipose triglyceride lipase were upregulated,while APOE,complement C3,clusterin,and IgG were downregulated.Furthermore,from 1 to 90 d,annexin A1,annexin A2,and antithrombin-ll were downregulated,then upregulated by d 240.Albumin had a high degree of connectedness,indicating that it was a key protein,according to protein-protein interaction research.Overall,our findings gave new insights into the biological features of MFGM protein in goat milk throughout lactation,which may aid in the creation of specialized MFGM products and infant formula.
基金funded by major projects in dairy products and meat processing technology provided by Government of Heilongjiang Province of the People’s Republic of China(No.2019ZX07B01).
文摘Proteomics comparison of human milk fat globular membrane proteins collected from Korean and Han ethnic groups in China was performed using data independent acquisition (DIA) technique. Nearly 1596 proteins were identified and they were mainly distributed in single-organism process/cellular process of biological process (BP), cell/cell part of cellular component (CC), and binding of molecular function (MF) in Gene ontology (GO) annotation and metabolic pathway in Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. A total of 238 differentially expressed proteins (DEPs) including 72 enzymes were detected between the two ethnic groups and 142 were higher in Korean women milk. Enrichment analysis showed that 374 GO functions and 118 pathways where DEPs participated were significantly enriched (q < 0.05). Protein-protein interaction (PPI) network denoted that the DEP of actin, cytoplasmic 1 interacted most with others. Data unraveled variations in proteins between different ethnic human milks and provided useful information for development of infant formula products.
