Metabolic syndrome(Met S)is a chronic disease associated with the disturbance of gut microbiota homeostasis.Metabolites derived from gut microbes play essential roles in Met S prevention and therapy.Here,we focused on...Metabolic syndrome(Met S)is a chronic disease associated with the disturbance of gut microbiota homeostasis.Metabolites derived from gut microbes play essential roles in Met S prevention and therapy.Here,we focused on the inhibitory effect of the extract of millet bran protein(EMBP)on a high-fat diet(HFD)-induced Met S,aiming to identify gut microbiota and their metabolites that involve in the anti-Met S activity of EMBP.The obesity,chronic inflammation,insulin resistance in Met S mouse models were abolished after EMBP treatment.The protective mechanism of EMBP against HFD-induced Met S may depend on improved gut barrier function.Using microbiome analysis,we found that EMBP supplementation improved gut microbiome dysbiosis in Met S mice,specifically upregulating Bacteroides acidifaciens.The fecal microbiota transplantation(FMT)also demonstrated this phenomenon.In addition,metabolomic analysis showed that EMBP mediates metabolic profiling reprogramming in Met S mice.Notably,a microbiota-derived metabolite,gamma-aminobutyric acid(GABA),is enriched by EMBP.In addition,exogenous GABA treatment produced a similar protective effect to EMBP by improving NRF2-dependent gut barrier function to protect HFDinduced Met S.The results suggest that EMBP suppress host Met S by remodeling of gut microbiota as an effective candidate for next-generation medicine food dual purpose dietary supplement to intervene in MetS.展开更多
目的:研究小米油与大豆蛋白复合饮料的降血脂作用。方法:观察高脂模型大鼠饮用不同剂量的小米油复合饮料后的血脂水平变化。结果:与模型组2相比,在小米油复合饮料的三个剂量中,高剂量(1.5 m L/100 g·d)的降脂作用最佳,血清甘油三酯...目的:研究小米油与大豆蛋白复合饮料的降血脂作用。方法:观察高脂模型大鼠饮用不同剂量的小米油复合饮料后的血脂水平变化。结果:与模型组2相比,在小米油复合饮料的三个剂量中,高剂量(1.5 m L/100 g·d)的降脂作用最佳,血清甘油三酯(TG)和胆固醇(TC)均极显著降低(p<0.01),高密度脂蛋白胆固醇(HDL-C)显著升高(p<0.05);中剂量(0.75 m L/100 g·d)组的TC极显著降低(p<0.01)、TG显著降低和HDL-C显著升高(p<0.05);低剂量(0.30 m L/100 g·d)组的TC虽显著降低(p<0.05),但TG和HDL-C变化不显著,降血脂效果较差。结论:该小米油复合饮料在一定剂量范围内有较好的降血脂作用。展开更多
基金supported by National Natural Science Foundation of China(32270420,32072220)National Key Research and Development Project(2020YFD1001405)+2 种基金Shanxi Province Science Foundation(202103021224011)Shanxi Key Laboratory for Research and Development of Regional PlantsShanxi Province“136”Revitalization Medical Project Construction Funds。
文摘Metabolic syndrome(Met S)is a chronic disease associated with the disturbance of gut microbiota homeostasis.Metabolites derived from gut microbes play essential roles in Met S prevention and therapy.Here,we focused on the inhibitory effect of the extract of millet bran protein(EMBP)on a high-fat diet(HFD)-induced Met S,aiming to identify gut microbiota and their metabolites that involve in the anti-Met S activity of EMBP.The obesity,chronic inflammation,insulin resistance in Met S mouse models were abolished after EMBP treatment.The protective mechanism of EMBP against HFD-induced Met S may depend on improved gut barrier function.Using microbiome analysis,we found that EMBP supplementation improved gut microbiome dysbiosis in Met S mice,specifically upregulating Bacteroides acidifaciens.The fecal microbiota transplantation(FMT)also demonstrated this phenomenon.In addition,metabolomic analysis showed that EMBP mediates metabolic profiling reprogramming in Met S mice.Notably,a microbiota-derived metabolite,gamma-aminobutyric acid(GABA),is enriched by EMBP.In addition,exogenous GABA treatment produced a similar protective effect to EMBP by improving NRF2-dependent gut barrier function to protect HFDinduced Met S.The results suggest that EMBP suppress host Met S by remodeling of gut microbiota as an effective candidate for next-generation medicine food dual purpose dietary supplement to intervene in MetS.
文摘目的:研究小米油与大豆蛋白复合饮料的降血脂作用。方法:观察高脂模型大鼠饮用不同剂量的小米油复合饮料后的血脂水平变化。结果:与模型组2相比,在小米油复合饮料的三个剂量中,高剂量(1.5 m L/100 g·d)的降脂作用最佳,血清甘油三酯(TG)和胆固醇(TC)均极显著降低(p<0.01),高密度脂蛋白胆固醇(HDL-C)显著升高(p<0.05);中剂量(0.75 m L/100 g·d)组的TC极显著降低(p<0.01)、TG显著降低和HDL-C显著升高(p<0.05);低剂量(0.30 m L/100 g·d)组的TC虽显著降低(p<0.05),但TG和HDL-C变化不显著,降血脂效果较差。结论:该小米油复合饮料在一定剂量范围内有较好的降血脂作用。
