[Objectives]The paper was to establish the quality standard of Yi herb medicine Millettia dielsiana.[Methods]Yi herb medicine M.dielsiana was qualitatively identified by microscopic identification and thin layer ident...[Objectives]The paper was to establish the quality standard of Yi herb medicine Millettia dielsiana.[Methods]Yi herb medicine M.dielsiana was qualitatively identified by microscopic identification and thin layer identification.The content of total isoflavones was determined by UV-Vis spectrophotometry,and the contents of moisture,total ash,acid-insoluble ash and extract were checked according to the method introduced in Chinese Pharmacopoeia(2020 edition).[Results]The microscopic identification features,including stone cells,fiber bundles,calcium oxalate square crystals,starch granules,pigment blocks,ducts,etc.,were distinct.A TLC method for identification of M.dielsiana was established.The contents of water,total ash,acid-insoluble ash and extract should be no more than 13%,no more than 7%,no more than 0.5%and no less than 6%in 10 batches of samples,respectively.The total isoflavones of M.dielsiana based on genistein(C_(15)H_(10)O_(5)) should be no less than 0.25%.The linear relationship of genistein was good in the range of 0.002-0.007 mg/mL(R^(2)=0.9996);the average recovery was 97.01%and the RSD was 2.62%.[Conclusions]The method is simple,accurate and well-reproducible,and can be used for the quality control of Yi herb medicine M.dielsiana.展开更多
香花崖豆藤(Millettia dielsiana Harms ex Diels)的藤茎可作鸡血藤用。本研究从中分离出七个结晶性成分。经物理常数,波谱分析确定它们分别为:(Ⅰ)羽扇烯酮;(Ⅱ)木栓酮;(Ⅲ)表木栓醇(Ⅳ)豆甾醇;(Ⅴ)β-谷甾醇;(Ⅵ)异甘草素;(Ⅶ)2′,4′...香花崖豆藤(Millettia dielsiana Harms ex Diels)的藤茎可作鸡血藤用。本研究从中分离出七个结晶性成分。经物理常数,波谱分析确定它们分别为:(Ⅰ)羽扇烯酮;(Ⅱ)木栓酮;(Ⅲ)表木栓醇(Ⅳ)豆甾醇;(Ⅴ)β-谷甾醇;(Ⅵ)异甘草素;(Ⅶ)2′,4′,3,4—四羟基查尔酮。其中化合物(Ⅰ),(Ⅵ),(Ⅶ)为首次从该植物中分离得到。展开更多
基金Supported by National Key Research and Development Program of China(2018YFC1706101)Science and Technology Program of Sichuan Province(Key Research and Development Project)(2021YFS0043)+2 种基金Standard Improvement of Traditional Chinese Medicine(Ethnic Medicine)of Sichuan Provincial Drug Administration(510201202102305)2022 Leading Talents Support Program of National Ethnic Affairs CommissionUniversity-level Innovation Team Special Project of Basic Research Funds for Central Universities and Colleges(ZYN2022067)。
文摘[Objectives]The paper was to establish the quality standard of Yi herb medicine Millettia dielsiana.[Methods]Yi herb medicine M.dielsiana was qualitatively identified by microscopic identification and thin layer identification.The content of total isoflavones was determined by UV-Vis spectrophotometry,and the contents of moisture,total ash,acid-insoluble ash and extract were checked according to the method introduced in Chinese Pharmacopoeia(2020 edition).[Results]The microscopic identification features,including stone cells,fiber bundles,calcium oxalate square crystals,starch granules,pigment blocks,ducts,etc.,were distinct.A TLC method for identification of M.dielsiana was established.The contents of water,total ash,acid-insoluble ash and extract should be no more than 13%,no more than 7%,no more than 0.5%and no less than 6%in 10 batches of samples,respectively.The total isoflavones of M.dielsiana based on genistein(C_(15)H_(10)O_(5)) should be no less than 0.25%.The linear relationship of genistein was good in the range of 0.002-0.007 mg/mL(R^(2)=0.9996);the average recovery was 97.01%and the RSD was 2.62%.[Conclusions]The method is simple,accurate and well-reproducible,and can be used for the quality control of Yi herb medicine M.dielsiana.
文摘香花崖豆藤(Millettia dielsiana Harms ex Diels)的藤茎可作鸡血藤用。本研究从中分离出七个结晶性成分。经物理常数,波谱分析确定它们分别为:(Ⅰ)羽扇烯酮;(Ⅱ)木栓酮;(Ⅲ)表木栓醇(Ⅳ)豆甾醇;(Ⅴ)β-谷甾醇;(Ⅵ)异甘草素;(Ⅶ)2′,4′,3,4—四羟基查尔酮。其中化合物(Ⅰ),(Ⅵ),(Ⅶ)为首次从该植物中分离得到。
