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题名对我国西部矿产资源综合评价指标体系的构建
被引量:14
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作者
陈莲芳
张富有
严良
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机构
中国地质大学(武汉)经济管理学院
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出处
《中国矿业》
北大核心
2008年第10期24-26,共3页
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基金
国家自然科学基金项目(70572016)
国家软科学研究计划项目(2005DGQ4B117)
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文摘
我国西部矿产资源集中区建设,对推动我国西部发展具有重要的现实意义。集中区综合评价,有助于把握集中区发展现状、存在的问题,找出影响集中区发展的因素,并为西部矿产资源特区选择提供依据。以系统论为基石,综合考虑影响指标体系构建的有关方面,构建了我国西部矿产资源集中区综合评价指标体系。
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关键词
西部矿产资源集中区
综合评价指标体系
构建
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Keywords
mineral resources collective areas in chinese western region
comprehensive evaluation index system
construction
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分类号
TD98
[矿业工程—选矿]
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题名中韩野生软枣猕猴桃种质资源遗传多样性分析
被引量:11
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作者
赵成日
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机构
延边大学农学院
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出处
《果树学报》
CAS
CSCD
北大核心
2018年第9期1043-1051,共9页
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基金
国家自然科学基金(31660319)
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文摘
【目的】利用RAPD分子标记技术研究国内长白山地区和韩国由来野生软枣猕猴桃种质资源的遗传多样性。【方法】以长白山地区和韩国由来的28个野生软枣猕猴桃的叶片为材料,利用RAPD分子标记技术进行了遗传多样性分析,并明确了它们之间的亲缘关系。【结果】利用131个随机引物进行PCR,从中筛选出了多态性高、重复性好且扩增条带清晰的24个引物。24个引物在28个野生软枣猕猴桃中共扩增出191条带,其中多态性条带为186条,占97.4%。平均每个引物产生7.75个多态性条带。应用NTSYSpc 2.10e软件进行遗传一致度和遗传距离分析后用UPGMA方法进行聚类分析。28个野生软枣猕猴桃种质资源之间的遗传距离为0.020 2~0.934 2。遗传距离0.58时可将28个野生种划分成2大类。在遗传距离最小的0.02处,有蛟河2号和3号2个野生种,其RAPD分子标记相似性为98%。【结论】来自不同地理区域的野生软枣猕猴桃之间存在较高的遗传多样性,而在同一地理区域内遗传多样性较低。韩国野生软枣猕猴桃之间的遗传多样性较低,且与二道白河、汪清、左家等地的野生软枣猕猴桃亲缘关系较近。即具有同一地理区域聚类趋势,且不同地理区域间存在较高的遗传多样性。
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关键词
软枣猕猴桃
遗传多样性
RAPD
长白山地区
韩国
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Keywords
Actinidia arguta
Gendenaturation at 94 ℃ for 1 min
annealing at 36 ℃for 1 min
extension at 72 ℃ for 2 min
reaction of 40 cycles
last extension at 72 ℃ for 5 min. The same template was repeated twice. RAPD-PCR amplification products were analyzed by 0.8% agarose gel (0.5 ×TAE buffer) electrophoresis. [Results]The 24 primers had high genetic polymorphisms. A total of 191 bands were amplified
of which 186 were polymorphic bands
accounting for 97.4%. Average 7.75 polymorphic bands were produced by each primer. Genetic identity and genetic distance were calculated by NTSYSpc 2.10e software and cluster analysis was analyzed by UPGMA. The result showed that the genetic distances among the 28 wild species ofA. arguta germplasm resources were 0.020 2-0.934 2. When the genetic distance was 0.58
the 28 wild species could be divided into two groups. The first group included Antu coloring type
Antu No.1-6 fromAntu County and Jiaohe No. 1-5 from Jiaohe. The second group include: ErdaobaiheNo. 1-4 from Erdaobaihe
Wangqing No. 1-3 from Wangqing County and Wangqing Forestry Bureau
Zuojia No. 1-3 from the Zuojia special production institute of the chinese Academy of Agricultural Sciences (CAAS)
Liaoning Huanren and Helong Qingshan
South Korea No. 1-4 from Chungbuk
Jeon- ham and Gyeongnam in South Korea. The similarity of RAPD markers of Jiaohe 2 and 3
the two wild species reached 98 % when the genetic distance was 0.0202. Almost all the wild species from the same area belonged to the same category within a very small genetic distance. For example
Antu No. 1-6 were within the 0.184 genetic distance
Jiaohe No. 1-5 were within 0.18 genetic distance
Wangqing No. 1-3 and Erdaobaihe No. 1 and No. 4 were within 0.315 genetic distance
Zuojia No. 1-3 was within 0.25 genetic distance
South Korea No. 1-4 were within 0.23 genetic distance
Erdaobaihe No. 2 and No. 3 were within 0.41 genetic distance. [Conclusion]There was a high genetic diversity among wild A. arguta collected from different geographic regions
but genetic diversity among those collected from the same geographical area was low. The genetic diversity of the wild A. arguta from South Korea was relatively low and was closely related to the wild A. arguta from Erdaobaihe
Wang Qing and Zuo Jia. The species collected from the etic diversity
RAPD
Changbai mountion
South Korea
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分类号
S663.4
[农业科学—果树学]
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