Objective:To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet(Z.zerumbet)(L.)Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi.Methods:The ...Objective:To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet(Z.zerumbet)(L.)Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi.Methods:The fresh rhizomes of Zingiber zerumbet were extracted in cold with ethanol(4.0 L)after concentration.The crude ethanol extract was fractionated by petroleum ether and chloroform to form a suspension of ethanol extract(15.0 g),petroleum ether fraction(6.6 g)and chloroform soluble fraction(5.0 g).The crude ethanol extract and its petroleum ether and chloroform fractions were evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and three fungi by the disc diffusion method.Commercially available kanamycin(30μg/disc)was used as standard disc and blank discs impregnated with the respective solvents were used as negative control.Results:At a concentration of 400μg/disc,all the samples showed mild to moderate antibacterial and antifungal activity and produced the zone of inhibition ranging from 6 mm to 10 mm.Among the tested samples,the crude ethanol extract showed the highest activity against Vibrio parahemolyticus(V.parahemolyticus).The minimum inhibitory concentration(MIC)of the crude ethanol extract and its fractions were within the value of 128-256μg/mL against two Gram positive and four Gram negative bacteria and all the samples showed the lowest MIC value against V.parahemolyticus(128μg/mL).Conclusions:It can be concluded that,potent antibacterial and antifungal phytochemicals are present in ethanol extract of Z.zerumbet(L.).展开更多
Objective:To explore the antibiotic resistance of Brucella melitensis and instruct rational use of antimicrobial agents in clinical treatment of Brucella infection.Methods:Bacteria were cultured and identified by BACT...Objective:To explore the antibiotic resistance of Brucella melitensis and instruct rational use of antimicrobial agents in clinical treatment of Brucella infection.Methods:Bacteria were cultured and identified by BACTEC9120 and VTTEK Ⅱ automicrobic system.E-test was used to detect the minimal inhibitory concentration(MIC) of antimicrobial agents in the drug susceptivity experiment.Results:A total of 19 brucella strains(all Brucella melitensis) were isolated from 19 patients,who had fever between January 2010 and June 2012,and 17 samples were blood,one was bone marrow,the other sample was cerebrospinal fluid.The MIC range of ceftazidime was 2.0-8.0 mg/L,rifampicin was 0.06-2.0 mg/L,amikacin was 4.0-12.0 mg/L,levofloxacin was 2.0-8.0 mg/ L,doxycycline was 8.0-32.0 mg/L,sulfamethoxazole-trimethoprim was 4.0-16.0 mg/L,ampicillin was 1.5-2.0 mg/L and gentamicin was 0.50-0.75 mg/L.Conclusions:The drugs used in this experiment cover common drugs for treating Brcella.Meanwhile,the results are consistent with clinical efficacy.It is suggested personalized regimen according to patients' status in treatment of Brucella.展开更多
Objective:To explore the antibacterial effect of Chinese crude drugs against clinical strains of Ureaplasma urealyticum(UU), including eight pure herbs and three compound herbs, and determine their minimal inhibito...Objective:To explore the antibacterial effect of Chinese crude drugs against clinical strains of Ureaplasma urealyticum(UU), including eight pure herbs and three compound herbs, and determine their minimal inhibitory concentrations(MICs). Methods:Isolates were collected from clinical patients with UU infection, and cultured in UU broth. In order to test the different effects on clinical strains of UU, the assays were performed by microdilution inhibition tests, and MICs of the herbs against the clinical strains of UU were calculated. Results:The MICs of eight pure herbs against clinical strains of UU were as follows: Galla Chinensis:0.313-1.25 g/L; Fructus Forsythiae: 1.25-5.00 g/L; Cortex Phellodendri: 1.25-5.00 g/L; Radix Paeoniae Rubra: 1.25-2.50 g/L; Semen Plantaginis:2.50-10.00 g/L; Herba Lysimachiae: 5.00-20.00 g/L; Rhizoma Coptidis: 5.00-20.00g/L, Herba Houttuyniae: 10.00-20.00 g/L. The MICs of compound herbs were: Liuheji: 0.625-2.50 g/L; Bazhengsan: 5.00-20.00 g/L; Wulinsan:2.50-20.0 g/L.Conclusion:Galla Chinensis, Fructus Forsythiae, Cortex Phellodendrim, Radix Paeoniae Rubra, and Semen Plantaginis, exerted the stronger antibacterial effect against clinical strains of UU, whereas Herba Lysimachiae, Rhizoma Coptidis and Herba Houttuyniae, had relatively weaker activity against UU. Compound herbs, Bazhengsan and Wulinsan, and particularly Liuheji, also had antibacterial effects against UU. Further studies of the effects and mechanisms of action of Chinese crude drugs against UU infections are worthwhile.展开更多
Melastoma malabathricum Linn. is a shrub that belongs to the family Melastomataceae and a common herbal plant used in folk medicines to treat inflamed wounds. This study was carried out with the aim to evaluate the in...Melastoma malabathricum Linn. is a shrub that belongs to the family Melastomataceae and a common herbal plant used in folk medicines to treat inflamed wounds. This study was carried out with the aim to evaluate the inhibitory activities of different concentrations of the M. malabathricum Linn. flower and fruit crude extracts against a variety of microorganisms. The inhibitory effects of both extracts were tested against the microorganisms using the disc diffusion method. The lowest concentrations of the extracts producing inhibition zones against the test microorganisms were used to determine their Minimum Inhibitory Concentrations (MICs) and Minimum Microbicidal Concentrations (MMCs). Both crude extracts showed strong inhibitory activities against Gram-positive bacteria. The range of MIC values for the crude flower and fruit extracts on all the bacteria tested were 12.5 to 100.0 mg/ml. Overall, Gram-positive bacteria were more susceptible to the crude extracts compared to Gram-negative species, potentiating a possible use of the extracts to inhibit or kill potential pathogens.展开更多
[Objectives] This study was conducted to explore the antifungal activity of wood vinegar produced from apricot shell. [Methods]The minimal inhibitory concentration( MIC) of wood vinegar against Aspergillus fumigatus...[Objectives] This study was conducted to explore the antifungal activity of wood vinegar produced from apricot shell. [Methods]The minimal inhibitory concentration( MIC) of wood vinegar against Aspergillus fumigatus was determined on agar plates. Then,the mycelial growth and the morphology of the apical vesicles of A. fumigatus cultured in the medium supplemented with 1/2 MIC wood vinegar were observed to evaluate the effect of wood vinegar on A. fumigatus.RNA-Seq was performed to explore the antifungal mechanism of wood vinegar. [Results] The wood vinegar produced from apricot shell could inhibit the growth of A. fumigatus,with a MIC of 20 μl/ml. In the medium supplemented with 1/2 MIC wood vinegar,the mycelial growth of A. fumigatus was suppressed,some apical vesicles were incompletely developed. The gene ontology( GO) term enrichment analysis based on RNA-Seq showed that the differentially expressed genes( DEGs)were enriched in metabolic process,cellular process and single-organism process in the category of biological processes,cell,cell part,membrane,organelle and membrane part in the category of cellular components,catalytic activity and binding in the category of molecular function. The significantly up-regulated DEGs were mostly involved in cell,cell part,organelle,organelle part and macro molecular complex in the category of cellular component,while the significantly down-regulated DEGs were mostly involved in biological process,cellular process and single-organism process in the category of biological process,catalytic activity,binding,transporter activity,and nucleic acid binding transcription factor activity in the category molecular function. Moreover,KEGG( Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed that the DEG unigenes involved in metabolism were the most,followed by those involved in genetic information processing.[Conclusions]The results indicated that the wood vinegar produced from apricot shell might inhibit the growth of A. fumigatus by interfering with the expression of multiple genes involved in multiple signaling pathways.展开更多
The purpose of this study was to first evaluate the antimicrobial effects of powder and extracts of berries (rose-hip, aronia, sea buckthorn and hawthorn) on the development of antibiotic-resistant <i><span s...The purpose of this study was to first evaluate the antimicrobial effects of powder and extracts of berries (rose-hip, aronia, sea buckthorn and hawthorn) on the development of antibiotic-resistant <i><span style="font-family:Verdana;">L</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">monocitogenes</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogenes</span></i><span style="font-family:Verdana;"> is considered one of the most important pathogens responsible for food-borne infection. Antimicrobial properties were evaluated using the standard Kirby-Bauer disk diffusion method. Bacterial inactivation networks were determined and compared, as well as the possibility of using powders and extracts of berries to control the risk of </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogees</span></i><span style="font-family:Verdana;"> infestation in the milk and dairy industry as well as in the meat industry. The effect of pH (4.78 - 4.43) and water activity (0.90 - 0.80) on the relationship between optical density (OD) at 600 nm and the plate count (CFU ml<sup>-</sup></span><span style="font-family:Verdana;"><sup>1</sup></span><span style="font-family:Verdana;">) was investigated for </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogenes</span></i><span style="font-family:Verdana;">. It was determined Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) </span><span style="font-family:Verdana;">of berries for</span><span style="font-family:;" "=""> </span><i><span style="font-family:Verdana;">L. monocytogenes</span></i><span style="font-family:Verdana;">. </span><span style="font-family:Verdana;">The most relevant bacteriostatic and bactericidal </span><span style="font-family:;" "=""><span style="font-family:Verdana;">effect on </span><i><span style="font-family:Verdana;">L. monocytogenes</span></i><span style="font-family:Verdana;"> in the tested berries demonstrated sea buckthorn and rosehip.</span></span>展开更多
Multiple drug resistant(MDR)and methicillin-resistant Staphylococcus aureus(MRSA)have become increasingly prevalent as a community acquired infection.As a result limited treatment options are available with convention...Multiple drug resistant(MDR)and methicillin-resistant Staphylococcus aureus(MRSA)have become increasingly prevalent as a community acquired infection.As a result limited treatment options are available with conventional synthetic antibiotics.Bioprospecting natural products with potent antimicrobial activity show promise for developing new drugs against this pathogen.In this study,we have investigated the antimicrobial activity of a purple violet pigment(PVP)from an Antarctic bacterium,Janthinobacterium sp.Ant5-2 on 15 clinical MDR and MRSA strains.The colorimetric resazurin assay was employed to determine the minimum inhibitory concentration(MIC90)of PVP against MDR and MRSA.The MIC90 ranged between 1.57μg/mL and 3.13μg/mL,which are significantly lower than many antimicrobials tested from natural sources against this pathogen.The spectrophotometrically determined growth analysis and total microscopic counts using Live/dead®BacLight™fluorescent stain exhibited a steady decrease in viability of both MDR and MRSA cultures following treatment with PVP at the MIC levels.In silico predictive molecular docking study revealed that PVP could be a DNA-targeting minor groove binding antimicrobial compound.The continued development of novel antimicrobials derived from natural sources with the combination of a suite of conventional antibiotics could stem the rising pandemic of MDR and MRSA along with other deadly microbial pathogens.展开更多
Although nowadays there are methods for determining the Minimal Bactericidal Concentration and Minimal Fungicidal Concentration, it is indispensable that?the establishment of innovative methodologies?could be more pra...Although nowadays there are methods for determining the Minimal Bactericidal Concentration and Minimal Fungicidal Concentration, it is indispensable that?the establishment of innovative methodologies?could be more practical and cheaper. The new methodology Flash microbiocide is an assay in which one aliquot from 96 well plate of Minimal Inhibitory Concentration test is transferred to another plate containing different culture medium. The correspondence with the reference methods described in the National Committee for Clinical Laboratory Standards (NCCLS-CLSI) document M26-A was achieved, denoting the efficiency of this fast and simple method.展开更多
基金Supported by Innovation Technology,Research and Development Project,Ministry of Science.Information and Communication Technology.Republic of Bangladesh Government(No.12)
文摘Objective:To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet(Z.zerumbet)(L.)Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi.Methods:The fresh rhizomes of Zingiber zerumbet were extracted in cold with ethanol(4.0 L)after concentration.The crude ethanol extract was fractionated by petroleum ether and chloroform to form a suspension of ethanol extract(15.0 g),petroleum ether fraction(6.6 g)and chloroform soluble fraction(5.0 g).The crude ethanol extract and its petroleum ether and chloroform fractions were evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and three fungi by the disc diffusion method.Commercially available kanamycin(30μg/disc)was used as standard disc and blank discs impregnated with the respective solvents were used as negative control.Results:At a concentration of 400μg/disc,all the samples showed mild to moderate antibacterial and antifungal activity and produced the zone of inhibition ranging from 6 mm to 10 mm.Among the tested samples,the crude ethanol extract showed the highest activity against Vibrio parahemolyticus(V.parahemolyticus).The minimum inhibitory concentration(MIC)of the crude ethanol extract and its fractions were within the value of 128-256μg/mL against two Gram positive and four Gram negative bacteria and all the samples showed the lowest MIC value against V.parahemolyticus(128μg/mL).Conclusions:It can be concluded that,potent antibacterial and antifungal phytochemicals are present in ethanol extract of Z.zerumbet(L.).
基金This project was supported by the scientific Research Foundation of Shaanxi Province.China(Grant No.2009JQ4011)
文摘Objective:To explore the antibiotic resistance of Brucella melitensis and instruct rational use of antimicrobial agents in clinical treatment of Brucella infection.Methods:Bacteria were cultured and identified by BACTEC9120 and VTTEK Ⅱ automicrobic system.E-test was used to detect the minimal inhibitory concentration(MIC) of antimicrobial agents in the drug susceptivity experiment.Results:A total of 19 brucella strains(all Brucella melitensis) were isolated from 19 patients,who had fever between January 2010 and June 2012,and 17 samples were blood,one was bone marrow,the other sample was cerebrospinal fluid.The MIC range of ceftazidime was 2.0-8.0 mg/L,rifampicin was 0.06-2.0 mg/L,amikacin was 4.0-12.0 mg/L,levofloxacin was 2.0-8.0 mg/ L,doxycycline was 8.0-32.0 mg/L,sulfamethoxazole-trimethoprim was 4.0-16.0 mg/L,ampicillin was 1.5-2.0 mg/L and gentamicin was 0.50-0.75 mg/L.Conclusions:The drugs used in this experiment cover common drugs for treating Brcella.Meanwhile,the results are consistent with clinical efficacy.It is suggested personalized regimen according to patients' status in treatment of Brucella.
文摘Objective:To explore the antibacterial effect of Chinese crude drugs against clinical strains of Ureaplasma urealyticum(UU), including eight pure herbs and three compound herbs, and determine their minimal inhibitory concentrations(MICs). Methods:Isolates were collected from clinical patients with UU infection, and cultured in UU broth. In order to test the different effects on clinical strains of UU, the assays were performed by microdilution inhibition tests, and MICs of the herbs against the clinical strains of UU were calculated. Results:The MICs of eight pure herbs against clinical strains of UU were as follows: Galla Chinensis:0.313-1.25 g/L; Fructus Forsythiae: 1.25-5.00 g/L; Cortex Phellodendri: 1.25-5.00 g/L; Radix Paeoniae Rubra: 1.25-2.50 g/L; Semen Plantaginis:2.50-10.00 g/L; Herba Lysimachiae: 5.00-20.00 g/L; Rhizoma Coptidis: 5.00-20.00g/L, Herba Houttuyniae: 10.00-20.00 g/L. The MICs of compound herbs were: Liuheji: 0.625-2.50 g/L; Bazhengsan: 5.00-20.00 g/L; Wulinsan:2.50-20.0 g/L.Conclusion:Galla Chinensis, Fructus Forsythiae, Cortex Phellodendrim, Radix Paeoniae Rubra, and Semen Plantaginis, exerted the stronger antibacterial effect against clinical strains of UU, whereas Herba Lysimachiae, Rhizoma Coptidis and Herba Houttuyniae, had relatively weaker activity against UU. Compound herbs, Bazhengsan and Wulinsan, and particularly Liuheji, also had antibacterial effects against UU. Further studies of the effects and mechanisms of action of Chinese crude drugs against UU infections are worthwhile.
文摘Melastoma malabathricum Linn. is a shrub that belongs to the family Melastomataceae and a common herbal plant used in folk medicines to treat inflamed wounds. This study was carried out with the aim to evaluate the inhibitory activities of different concentrations of the M. malabathricum Linn. flower and fruit crude extracts against a variety of microorganisms. The inhibitory effects of both extracts were tested against the microorganisms using the disc diffusion method. The lowest concentrations of the extracts producing inhibition zones against the test microorganisms were used to determine their Minimum Inhibitory Concentrations (MICs) and Minimum Microbicidal Concentrations (MMCs). Both crude extracts showed strong inhibitory activities against Gram-positive bacteria. The range of MIC values for the crude flower and fruit extracts on all the bacteria tested were 12.5 to 100.0 mg/ml. Overall, Gram-positive bacteria were more susceptible to the crude extracts compared to Gram-negative species, potentiating a possible use of the extracts to inhibit or kill potential pathogens.
基金Supported by the Key Scientific and Technological Research Project for Universities of Hebei Province(ZD2018306)
文摘[Objectives] This study was conducted to explore the antifungal activity of wood vinegar produced from apricot shell. [Methods]The minimal inhibitory concentration( MIC) of wood vinegar against Aspergillus fumigatus was determined on agar plates. Then,the mycelial growth and the morphology of the apical vesicles of A. fumigatus cultured in the medium supplemented with 1/2 MIC wood vinegar were observed to evaluate the effect of wood vinegar on A. fumigatus.RNA-Seq was performed to explore the antifungal mechanism of wood vinegar. [Results] The wood vinegar produced from apricot shell could inhibit the growth of A. fumigatus,with a MIC of 20 μl/ml. In the medium supplemented with 1/2 MIC wood vinegar,the mycelial growth of A. fumigatus was suppressed,some apical vesicles were incompletely developed. The gene ontology( GO) term enrichment analysis based on RNA-Seq showed that the differentially expressed genes( DEGs)were enriched in metabolic process,cellular process and single-organism process in the category of biological processes,cell,cell part,membrane,organelle and membrane part in the category of cellular components,catalytic activity and binding in the category of molecular function. The significantly up-regulated DEGs were mostly involved in cell,cell part,organelle,organelle part and macro molecular complex in the category of cellular component,while the significantly down-regulated DEGs were mostly involved in biological process,cellular process and single-organism process in the category of biological process,catalytic activity,binding,transporter activity,and nucleic acid binding transcription factor activity in the category molecular function. Moreover,KEGG( Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed that the DEG unigenes involved in metabolism were the most,followed by those involved in genetic information processing.[Conclusions]The results indicated that the wood vinegar produced from apricot shell might inhibit the growth of A. fumigatus by interfering with the expression of multiple genes involved in multiple signaling pathways.
文摘The purpose of this study was to first evaluate the antimicrobial effects of powder and extracts of berries (rose-hip, aronia, sea buckthorn and hawthorn) on the development of antibiotic-resistant <i><span style="font-family:Verdana;">L</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">monocitogenes</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogenes</span></i><span style="font-family:Verdana;"> is considered one of the most important pathogens responsible for food-borne infection. Antimicrobial properties were evaluated using the standard Kirby-Bauer disk diffusion method. Bacterial inactivation networks were determined and compared, as well as the possibility of using powders and extracts of berries to control the risk of </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogees</span></i><span style="font-family:Verdana;"> infestation in the milk and dairy industry as well as in the meat industry. The effect of pH (4.78 - 4.43) and water activity (0.90 - 0.80) on the relationship between optical density (OD) at 600 nm and the plate count (CFU ml<sup>-</sup></span><span style="font-family:Verdana;"><sup>1</sup></span><span style="font-family:Verdana;">) was investigated for </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogenes</span></i><span style="font-family:Verdana;">. It was determined Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) </span><span style="font-family:Verdana;">of berries for</span><span style="font-family:;" "=""> </span><i><span style="font-family:Verdana;">L. monocytogenes</span></i><span style="font-family:Verdana;">. </span><span style="font-family:Verdana;">The most relevant bacteriostatic and bactericidal </span><span style="font-family:;" "=""><span style="font-family:Verdana;">effect on </span><i><span style="font-family:Verdana;">L. monocytogenes</span></i><span style="font-family:Verdana;"> in the tested berries demonstrated sea buckthorn and rosehip.</span></span>
文摘Multiple drug resistant(MDR)and methicillin-resistant Staphylococcus aureus(MRSA)have become increasingly prevalent as a community acquired infection.As a result limited treatment options are available with conventional synthetic antibiotics.Bioprospecting natural products with potent antimicrobial activity show promise for developing new drugs against this pathogen.In this study,we have investigated the antimicrobial activity of a purple violet pigment(PVP)from an Antarctic bacterium,Janthinobacterium sp.Ant5-2 on 15 clinical MDR and MRSA strains.The colorimetric resazurin assay was employed to determine the minimum inhibitory concentration(MIC90)of PVP against MDR and MRSA.The MIC90 ranged between 1.57μg/mL and 3.13μg/mL,which are significantly lower than many antimicrobials tested from natural sources against this pathogen.The spectrophotometrically determined growth analysis and total microscopic counts using Live/dead®BacLight™fluorescent stain exhibited a steady decrease in viability of both MDR and MRSA cultures following treatment with PVP at the MIC levels.In silico predictive molecular docking study revealed that PVP could be a DNA-targeting minor groove binding antimicrobial compound.The continued development of novel antimicrobials derived from natural sources with the combination of a suite of conventional antibiotics could stem the rising pandemic of MDR and MRSA along with other deadly microbial pathogens.
基金supported by University of Ribeirao Preto(UNAERP),FAPESP 2010/07970-7 and Ourofino.
文摘Although nowadays there are methods for determining the Minimal Bactericidal Concentration and Minimal Fungicidal Concentration, it is indispensable that?the establishment of innovative methodologies?could be more practical and cheaper. The new methodology Flash microbiocide is an assay in which one aliquot from 96 well plate of Minimal Inhibitory Concentration test is transferred to another plate containing different culture medium. The correspondence with the reference methods described in the National Committee for Clinical Laboratory Standards (NCCLS-CLSI) document M26-A was achieved, denoting the efficiency of this fast and simple method.