The importance of immunoglobulin Y(IgY) as a specific antibody equivalent to mammalian immunoglobulin G(IgG) is well recognized. However, production of highly purified IgY is still difficult due to the lack of specifi...The importance of immunoglobulin Y(IgY) as a specific antibody equivalent to mammalian immunoglobulin G(IgG) is well recognized. However, production of highly purified IgY is still difficult due to the lack of specific purification methods. In this study, adsorption behaviors of Ig Y on four mixed-mode resins with functional ligands of 4-mercatoethyl-pyridine(MEP), 2-mercapto-1-methyli-midazole(MMI), 5-aminobenzi-midazole(ABI) and tryptophan-5-aminobenzi-midazole(W-ABI) were evaluated. The results showed that high adsorption ratio were found at p H 6.0–7.0 with little adsorption under acidic conditions. The resin with ABI ligand was then used to separate IgY from immunized chicken serum. An efficient process with Ig Y purity of 95% and recovery of 90% was developed after optimization of loading and elution p H and injection volume. The biological activity of the purified Ig Y was fully maintained. These results indicated that mixed-mode chromatography with specially-designed ligands has great potential for the separation of Ig Y from crude feedstock.展开更多
used－silical capillary columns containing heptakis（2、3、6－tri－o－pentyl）-β-cyclodextrinand dibenzo-18-crown-6 were prepared．By studying the selectivity of mixed stationary phases forsome solute pairs.as well a...used－silical capillary columns containing heptakis（2、3、6－tri－o－pentyl）-β-cyclodextrinand dibenzo-18-crown-6 were prepared．By studying the selectivity of mixed stationary phases forsome solute pairs.as well as comparing with the heptakis（2．3、6-tri-O-pentyl）-β-cyclodextrin and thedibenzo-18-crown-6 used as individual stationary phase、the synergistic effects were observed.These effects were affected by the column temperature．mixed ratio and linear velocity of carrier gas．展开更多
A series of mixed mode tests were carried out on 18-8 stainless steel in boiling 42% MgCl_2 solution.The results show that for any K_Ⅱ/K_Ⅰ ratio,the SCC direction coin- cides well with the crack tip maximum normal s...A series of mixed mode tests were carried out on 18-8 stainless steel in boiling 42% MgCl_2 solution.The results show that for any K_Ⅱ/K_Ⅰ ratio,the SCC direction coin- cides well with the crack tip maximum normal stress plane,while the SCC resistance of the material reduces as the ratio of K_Ⅱ/K_Ⅰ increases.The experimental results were discussed in the light of anode dissolving mechanism and the effect of mixed mode loading on crack tip stress and strain.It is concluded that for fracture analysis if mixed mode cracks were simply taken into account as mode Ⅰ cracks,and only mode Ⅰ testing results as mode Ⅰ fracture criter- ion were employed,it may not be safe.展开更多
A reversed phase (RP)/hydrophilic interaction (HILIC)/ion exchange (IEX) mixed tri-mode stationary phase (TMSP) has been prepared via a divergent synthesis scheme starting from propylamine on silica then by amine-epox...A reversed phase (RP)/hydrophilic interaction (HILIC)/ion exchange (IEX) mixed tri-mode stationary phase (TMSP) has been prepared via a divergent synthesis scheme starting from propylamine on silica then by amine-epoxy reactions with 1,4-butanedioldiglycidyl ether and tertiary amines (N,Ndimethyldecylamine, DMDA). Its retention mechanism was found to follow RP/HILIC/IEX mixed-mode.The stop-flow test revealed that TMSP had good compatibility with 100% aqueous mobile phase. It demonstrated effective separation towards several kinds of compounds or drug molecules and their counterions within a single run.展开更多
采用反相和阳离子交换双重保留机理,无需添加离子对和衍生化试剂,建立了18种游离氨基酸的液相色谱-质谱联用直接分析方法。采用高效液相色谱-三重四极杆串联质谱(HPLC-MS/MS)系统,使用OSAKA SODA CAPCELL PAK CR 1∶4 (150 mm×2.1 ...采用反相和阳离子交换双重保留机理,无需添加离子对和衍生化试剂,建立了18种游离氨基酸的液相色谱-质谱联用直接分析方法。采用高效液相色谱-三重四极杆串联质谱(HPLC-MS/MS)系统,使用OSAKA SODA CAPCELL PAK CR 1∶4 (150 mm×2.1 mm, 5μm; SCX∶C_(18)=1∶4)色谱柱,以0.1%甲酸(A)-乙腈(含0.1%甲酸)(B)-50 mmol/L甲酸铵(C)为流动相进行三元梯度洗脱,对18种氨基酸进行分离检测,可得到良好的分析结果。色谱峰面积精密度(RSD)范围为0.7%～5.9%;标准曲线线性关系良好(R^2=0.993～0.999);对SD大鼠血清和尿液样品加样回收率范围为92.2%～113.6%。本方法流动相条件简单,灵敏度高,可用于血清和尿液中游离氨基酸分析,为生物样品中氨基酸检测或其它复杂基质中氨基酸的检测提供了新的方法和思路。展开更多
基金Supported by the National Natural Science Foundation of China(21776243,21476198)the International Science&Technology Cooperation Program of China(2015DFG42070)
文摘The importance of immunoglobulin Y(IgY) as a specific antibody equivalent to mammalian immunoglobulin G(IgG) is well recognized. However, production of highly purified IgY is still difficult due to the lack of specific purification methods. In this study, adsorption behaviors of Ig Y on four mixed-mode resins with functional ligands of 4-mercatoethyl-pyridine(MEP), 2-mercapto-1-methyli-midazole(MMI), 5-aminobenzi-midazole(ABI) and tryptophan-5-aminobenzi-midazole(W-ABI) were evaluated. The results showed that high adsorption ratio were found at p H 6.0–7.0 with little adsorption under acidic conditions. The resin with ABI ligand was then used to separate IgY from immunized chicken serum. An efficient process with Ig Y purity of 95% and recovery of 90% was developed after optimization of loading and elution p H and injection volume. The biological activity of the purified Ig Y was fully maintained. These results indicated that mixed-mode chromatography with specially-designed ligands has great potential for the separation of Ig Y from crude feedstock.
文摘used－silical capillary columns containing heptakis（2、3、6－tri－o－pentyl）-β-cyclodextrinand dibenzo-18-crown-6 were prepared．By studying the selectivity of mixed stationary phases forsome solute pairs.as well as comparing with the heptakis（2．3、6-tri-O-pentyl）-β-cyclodextrin and thedibenzo-18-crown-6 used as individual stationary phase、the synergistic effects were observed.These effects were affected by the column temperature．mixed ratio and linear velocity of carrier gas．
文摘A series of mixed mode tests were carried out on 18-8 stainless steel in boiling 42% MgCl_2 solution.The results show that for any K_Ⅱ/K_Ⅰ ratio,the SCC direction coin- cides well with the crack tip maximum normal stress plane,while the SCC resistance of the material reduces as the ratio of K_Ⅱ/K_Ⅰ increases.The experimental results were discussed in the light of anode dissolving mechanism and the effect of mixed mode loading on crack tip stress and strain.It is concluded that for fracture analysis if mixed mode cracks were simply taken into account as mode Ⅰ cracks,and only mode Ⅰ testing results as mode Ⅰ fracture criter- ion were employed,it may not be safe.
基金financially supported by the National Natural Science Foundation of China(Nos. 21477037, 21322502)the Outstanding Young Talent Cultivation Fund of East China University of Science and Technology
文摘A reversed phase (RP)/hydrophilic interaction (HILIC)/ion exchange (IEX) mixed tri-mode stationary phase (TMSP) has been prepared via a divergent synthesis scheme starting from propylamine on silica then by amine-epoxy reactions with 1,4-butanedioldiglycidyl ether and tertiary amines (N,Ndimethyldecylamine, DMDA). Its retention mechanism was found to follow RP/HILIC/IEX mixed-mode.The stop-flow test revealed that TMSP had good compatibility with 100% aqueous mobile phase. It demonstrated effective separation towards several kinds of compounds or drug molecules and their counterions within a single run.
文摘采用反相和阳离子交换双重保留机理,无需添加离子对和衍生化试剂,建立了18种游离氨基酸的液相色谱-质谱联用直接分析方法。采用高效液相色谱-三重四极杆串联质谱(HPLC-MS/MS)系统,使用OSAKA SODA CAPCELL PAK CR 1∶4 (150 mm×2.1 mm, 5μm; SCX∶C_(18)=1∶4)色谱柱,以0.1%甲酸(A)-乙腈(含0.1%甲酸)(B)-50 mmol/L甲酸铵(C)为流动相进行三元梯度洗脱,对18种氨基酸进行分离检测,可得到良好的分析结果。色谱峰面积精密度(RSD)范围为0.7%～5.9%;标准曲线线性关系良好(R^2=0.993～0.999);对SD大鼠血清和尿液样品加样回收率范围为92.2%～113.6%。本方法流动相条件简单,灵敏度高,可用于血清和尿液中游离氨基酸分析,为生物样品中氨基酸检测或其它复杂基质中氨基酸的检测提供了新的方法和思路。
