A novel complex [Co(phen)2HPlP]Cl3[phen= phenanethroline, HPIP=2-(2-hydroxyphenyl) imidazo [4,5-f][l,10]phenanethroline] has been synthesized and structurally characterized by elemental analysis, UV, IR and 1H NMR spe...A novel complex [Co(phen)2HPlP]Cl3[phen= phenanethroline, HPIP=2-(2-hydroxyphenyl) imidazo [4,5-f][l,10]phenanethroline] has been synthesized and structurally characterized by elemental analysis, UV, IR and 1H NMR spectroscopies. The interaction of the complex with calf thymus DNA (CT DNA) has been studied using absorption and emission spectroscopy, DNA melting techniques and cyclic voltammetry. The compound shows absorption hypochromicity, fluorescence enhancement and DNA melting temperature increment when binding to CT DNA. CV measurement shows a shift in reduction potential and a change in peak current with addition of DNA. These results prove mat the compound inserts into DNA base pairs. The shift of peak potential indicates the ion interaction mode between the complex and DNA. The binding constant of the compound to DNA is 4.37 × 104. The complex also seems to be an efficient photocleavage reagent.展开更多
基金theNationalNaturalScienceFoundationofChina (No .2 0 1710 31)andtheNaturalScienceFoundationofShanxiProvince (No .2 0 0 110 0 7)
文摘A novel complex [Co(phen)2HPlP]Cl3[phen= phenanethroline, HPIP=2-(2-hydroxyphenyl) imidazo [4,5-f][l,10]phenanethroline] has been synthesized and structurally characterized by elemental analysis, UV, IR and 1H NMR spectroscopies. The interaction of the complex with calf thymus DNA (CT DNA) has been studied using absorption and emission spectroscopy, DNA melting techniques and cyclic voltammetry. The compound shows absorption hypochromicity, fluorescence enhancement and DNA melting temperature increment when binding to CT DNA. CV measurement shows a shift in reduction potential and a change in peak current with addition of DNA. These results prove mat the compound inserts into DNA base pairs. The shift of peak potential indicates the ion interaction mode between the complex and DNA. The binding constant of the compound to DNA is 4.37 × 104. The complex also seems to be an efficient photocleavage reagent.
