Multidrug-resistant(MDR)Enterobacteriaceae critically threaten duck farming and public health.The phenotypes,genotypes,and associated mobile genetic elements(MGEs)of MDR Enterobacteriaceae isolated from 6 duck farms i...Multidrug-resistant(MDR)Enterobacteriaceae critically threaten duck farming and public health.The phenotypes,genotypes,and associated mobile genetic elements(MGEs)of MDR Enterobacteriaceae isolated from 6 duck farms in Zhejiang Province,China,were investigated.A total of 215 isolates were identified as Escherichia coli(64.65%),Klebsiella pneumoniae(12.09%),Proteus mirabilis(10.23%),Salmonella(8.84%),and Enterobacter cloacae(4.19%).Meanwhile,all isolates were resistant to at least two antibiotics.Most isolates carried tet(A)(85.12%),blaTEM(78.60%)and sul1(67.44%)resistance genes.Gene co-occurrence analysis showed that the resistance genes were associated with IS26 and integrons.A conjugative IncFII plasmid pSDM004 containing all the above MGEs was detected in Proteus mirabilis isolate SDM004.This isolate was resistant to 18 antibiotics and carried the blaNDM-5 gene.MGEs,especially plasmids,are the primary antibiotic resistance gene transmission route in duck farms.These findings provide a theoretical basis for the rational use of antibiotics in farms which are substantial for evaluating public health and food safety.展开更多
Exploiting mobile elements （MEs） to accomplish data collection in wireless sensor networks （WSNs） can improve the energy efficiency of sensor nodes, and prolong network lifetime. However, it will lead to large dat...Exploiting mobile elements （MEs） to accomplish data collection in wireless sensor networks （WSNs） can improve the energy efficiency of sensor nodes, and prolong network lifetime. However, it will lead to large data collection latency for the network, which is unacceptable for data-critical applications. In this paper, we address this problem by minimizing the traveling length of MEs. Our methods mainly consist of two steps： we first construct a virtual grid network and select the minimal stop point set （SPS） from it; then, we make optimal scheduling for the MEs based on the SPS in order to minimize their traveling length. Different implementations of genetic algorithm （GA） are used to solve the problem. Our methods are evaluated by extensive simulations. The results show that these methods can greatly reduce the traveling length of MEs, and decrease the data collection latency.展开更多
目的分析全球鼠伤寒沙门菌的流行和分子特点。方法从NCBI数据库批量下载全球沙门菌基因组和相应血清型及meta信息。使用在线软件ResFinder、PlasmidFinder、Mobile Element Finder和MLST分析抗生素耐药基因(ARGs)、质粒、移动遗传元件(M...目的分析全球鼠伤寒沙门菌的流行和分子特点。方法从NCBI数据库批量下载全球沙门菌基因组和相应血清型及meta信息。使用在线软件ResFinder、PlasmidFinder、Mobile Element Finder和MLST分析抗生素耐药基因(ARGs)、质粒、移动遗传元件(MGEs)在鼠伤寒沙门菌中的分布及其序列分型(STs)。结果323株鼠伤寒沙门菌中共检出101种ARGs,其中最常见的是aac(6′)-Iaa(322/323,99.69%),其次为sul2(155/323,47.99%)、aph(3″)-Ib(128/323,39.63%)、aph(6)-Id(127/323,39.32%)、tet(B)(109/323,33.75%)和blaTEM-1B(106/323,32.82%)。共检出36种质粒,以IncFⅡ(S)(110/323,34.06%)和IncFⅠB(S)(108/323,33.44%)最为常见。另外,鉴定到376个MGEs,包括367种插入序列和9种转座子,以MITEEc1(322/323,99.69%)和ISSen1(308/323,95.36%)最为流行。323株鼠伤寒沙门菌鉴定到32种不同的STs,其中,ST19(157/323,48.61%)和ST34(105/323,32.51%)是最为常见的2种STs,占82%以上。人类来源的115株细菌中鉴定出18种STs,也是以ST19(52/115,45.22%)和ST34(39/115,33.91%)最为常见。结论鼠伤寒沙门菌携带有多种类型的ARGs,并伴有大量插入序列和质粒的流行为耐药的流行播散提供了有利条件,针对该菌应加强感染防控措施。展开更多
细菌可移动遗传元件包括噬菌体、质粒、转座子、插入序列、整合子、基因组岛(genomic island and genomic islet)等,其中接合性质粒、转座子、整合子及基因组岛等是与抗生素抗性有关的元件,可以在同种甚至于不同种菌株间水平转移,加速...细菌可移动遗传元件包括噬菌体、质粒、转座子、插入序列、整合子、基因组岛(genomic island and genomic islet)等,其中接合性质粒、转座子、整合子及基因组岛等是与抗生素抗性有关的元件,可以在同种甚至于不同种菌株间水平转移,加速了临床上耐药及多重耐药菌株的产生。综述了细菌与抗生素抗性有关的可移动遗传元件的种类、特征及转移机制的研究进展。展开更多
基金supported by the National Natural Science Foundation of China(32172188)Science and Technology Cooperation Project of ZheJiang Province(2023SNJF058-3)。
文摘Multidrug-resistant(MDR)Enterobacteriaceae critically threaten duck farming and public health.The phenotypes,genotypes,and associated mobile genetic elements(MGEs)of MDR Enterobacteriaceae isolated from 6 duck farms in Zhejiang Province,China,were investigated.A total of 215 isolates were identified as Escherichia coli(64.65%),Klebsiella pneumoniae(12.09%),Proteus mirabilis(10.23%),Salmonella(8.84%),and Enterobacter cloacae(4.19%).Meanwhile,all isolates were resistant to at least two antibiotics.Most isolates carried tet(A)(85.12%),blaTEM(78.60%)and sul1(67.44%)resistance genes.Gene co-occurrence analysis showed that the resistance genes were associated with IS26 and integrons.A conjugative IncFII plasmid pSDM004 containing all the above MGEs was detected in Proteus mirabilis isolate SDM004.This isolate was resistant to 18 antibiotics and carried the blaNDM-5 gene.MGEs,especially plasmids,are the primary antibiotic resistance gene transmission route in duck farms.These findings provide a theoretical basis for the rational use of antibiotics in farms which are substantial for evaluating public health and food safety.
基金supported by Tianjin Municipal Information Industry Office (No. 082044012)
文摘Exploiting mobile elements （MEs） to accomplish data collection in wireless sensor networks （WSNs） can improve the energy efficiency of sensor nodes, and prolong network lifetime. However, it will lead to large data collection latency for the network, which is unacceptable for data-critical applications. In this paper, we address this problem by minimizing the traveling length of MEs. Our methods mainly consist of two steps： we first construct a virtual grid network and select the minimal stop point set （SPS） from it; then, we make optimal scheduling for the MEs based on the SPS in order to minimize their traveling length. Different implementations of genetic algorithm （GA） are used to solve the problem. Our methods are evaluated by extensive simulations. The results show that these methods can greatly reduce the traveling length of MEs, and decrease the data collection latency.
文摘目的分析全球鼠伤寒沙门菌的流行和分子特点。方法从NCBI数据库批量下载全球沙门菌基因组和相应血清型及meta信息。使用在线软件ResFinder、PlasmidFinder、Mobile Element Finder和MLST分析抗生素耐药基因(ARGs)、质粒、移动遗传元件(MGEs)在鼠伤寒沙门菌中的分布及其序列分型(STs)。结果323株鼠伤寒沙门菌中共检出101种ARGs,其中最常见的是aac(6′)-Iaa(322/323,99.69%),其次为sul2(155/323,47.99%)、aph(3″)-Ib(128/323,39.63%)、aph(6)-Id(127/323,39.32%)、tet(B)(109/323,33.75%)和blaTEM-1B(106/323,32.82%)。共检出36种质粒,以IncFⅡ(S)(110/323,34.06%)和IncFⅠB(S)(108/323,33.44%)最为常见。另外,鉴定到376个MGEs,包括367种插入序列和9种转座子,以MITEEc1(322/323,99.69%)和ISSen1(308/323,95.36%)最为流行。323株鼠伤寒沙门菌鉴定到32种不同的STs,其中,ST19(157/323,48.61%)和ST34(105/323,32.51%)是最为常见的2种STs,占82%以上。人类来源的115株细菌中鉴定出18种STs,也是以ST19(52/115,45.22%)和ST34(39/115,33.91%)最为常见。结论鼠伤寒沙门菌携带有多种类型的ARGs,并伴有大量插入序列和质粒的流行为耐药的流行播散提供了有利条件,针对该菌应加强感染防控措施。
文摘细菌可移动遗传元件包括噬菌体、质粒、转座子、插入序列、整合子、基因组岛(genomic island and genomic islet)等,其中接合性质粒、转座子、整合子及基因组岛等是与抗生素抗性有关的元件,可以在同种甚至于不同种菌株间水平转移,加速了临床上耐药及多重耐药菌株的产生。综述了细菌与抗生素抗性有关的可移动遗传元件的种类、特征及转移机制的研究进展。
