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3D‑Printed Carbon‑Based Conformal Electromagnetic Interference Shielding Module for Integrated Electronics 被引量:3
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作者 Shaohong Shi Yuheng Jiang +5 位作者 Hao Ren Siwen Deng Jianping Sun Fangchao Cheng Jingjing Jing Yinghong Chen 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第5期87-101,共15页
Electromagnetic interference shielding(EMI SE)modules are the core com-ponent of modern electronics.However,the tra-ditional metal-based SE modules always take up indispensable three-dimensional space inside electroni... Electromagnetic interference shielding(EMI SE)modules are the core com-ponent of modern electronics.However,the tra-ditional metal-based SE modules always take up indispensable three-dimensional space inside electronics,posing a major obstacle to the integra-tion of electronics.The innovation of integrating 3D-printed conformal shielding(c-SE)modules with packaging materials onto core electronics offers infinite possibilities to satisfy ideal SE func-tion without occupying additional space.Herein,the 3D printable carbon-based inks with various proportions of graphene and carbon nanotube nanoparticles are well-formulated by manipulating their rheological peculiarity.Accordingly,the free-constructed architectures with arbitrarily-customized structure and multifunctionality are created via 3D printing.In particular,the SE performance of 3D-printed frame is up to 61.4 dB,simultaneously accompanied with an ultralight architecture of 0.076 g cm^(-3) and a superhigh specific shielding of 802.4 dB cm3 g^(-1).Moreover,as a proof-of-concept,the 3D-printed c-SE module is in situ integrated into core electronics,successfully replacing the traditional metal-based module to afford multiple functions for electromagnetic compatibility and thermal dissipa-tion.Thus,this scientific innovation completely makes up the blank for assembling carbon-based c-SE modules and sheds a brilliant light on developing the next generation of high-performance shielding materials with arbitrarily-customized structure for integrated electronics. 展开更多
关键词 3D printing Carbon-based nanoparticles Conformal electromagnetic interference shielding Integrated electronics
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3D Printed Integrated Gradient-Conductive MXene/CNT/Polyimide Aerogel Frames for Electromagnetic Interference Shielding with Ultra-Low Reflection 被引量:18
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作者 Tiantian Xue Yi Yang +5 位作者 Dingyi Yu Qamar Wali Zhenyu Wang Xuesong Cao Wei Fan Tianxi Liu 《Nano-Micro Letters》 SCIE EI CAS CSCD 2023年第3期332-345,共14页
Construction of advanced electromagnetic interference(EMI)shielding materials with miniaturized,programmable structure and low reflection are promising but challenging.Herein,an integrated transition-metal carbides/ca... Construction of advanced electromagnetic interference(EMI)shielding materials with miniaturized,programmable structure and low reflection are promising but challenging.Herein,an integrated transition-metal carbides/carbon nanotube/polyimide(gradient-conductive MXene/CNT/PI,GCMCP)aerogel frame with hierarchical porous structure and gradient-conductivity has been constructed to achieve EMI shielding with ultra-low reflection.The gradient-conductive structures are obtained by continuous 3D printing of MXene/CNT/poly(amic acid)inks with different CNT contents,where the slightly conductive top layer serves as EM absorption layer and the highly conductive bottom layer as reflection layer.In addition,the hierarchical porous structure could extend the EM dissipation path and dissipate EM by multiple reflections.Consequently,the GCMCP aerogel frames exhibit an excellent average EMI shielding efficiency(68.2 dB)and low reflection(R=0.23).Furthermore,the GCMCP aerogel frames with miniaturized and programmable structures can be used as EMI shielding gaskets and effectively block wireless power transmission,which shows a prosperous application prospect in defense industry and aerospace. 展开更多
关键词 3D printing MXene/CNT/Polyimide aerogel Gradient-conductive Electromagnetic interference shielding
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Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)纳米复合材料的吸波和电磁屏蔽性能与机制 被引量:1
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作者 李月霞 吴梦 +3 位作者 纪子影 刘璐 应国兵 徐鹏飞 《材料导报》 EI CAS CSCD 北大核心 2024年第9期21-27,共7页
在电磁屏蔽领域,铁氧体是常用的涂覆型吸波剂,但以Fe_(3)O_(4)为首的铁氧体存在一些不足。本研究采用冷冻干燥的方法成功制备了花苞状Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)复合材料,Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)复合材料的花苞状结构对电磁波... 在电磁屏蔽领域,铁氧体是常用的涂覆型吸波剂,但以Fe_(3)O_(4)为首的铁氧体存在一些不足。本研究采用冷冻干燥的方法成功制备了花苞状Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)复合材料,Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)复合材料的花苞状结构对电磁波的多重反射、界面极化和电磁耦合作用等使复合材料具有更好的微波吸收性能。当频率为6.74 GHz时,最小反射损耗达到-51.41 dB,对应的匹配厚度为2.8 mm,这意味着它可以吸收99.99928%的电磁波。本研究中特殊的花苞状Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)复合材料表现出优异的吸波性能,在电磁屏蔽领域具有良好的应用前景。 展开更多
关键词 MXene Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)复合材料 吸波 电磁屏蔽 吸波剂
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Rice Repetitive DNA Sequence RRD3:a Plant Promoter and Its Application to RNA Interference
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作者 仲健 王宏斌 +2 位作者 张党权 刘兵 王金发 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第3期258-266,共9页
Previously, a moderately repetitive DNA sequence (RRD3) was cloned from rice (Oryza sativa L.) by DNA renaturation kinetics. Sequence analysis revealed several conserved promoter motifs, including four TATA-boxes ... Previously, a moderately repetitive DNA sequence (RRD3) was cloned from rice (Oryza sativa L.) by DNA renaturation kinetics. Sequence analysis revealed several conserved promoter motifs, including four TATA-boxes and a CAAT-box, and promoter activity was shown in Escherichia coli and mammalian expression systems. Here, we inserted the RRD3 fragment into the plant promoter-capture vector, pCAMBIA1391Z, and examined whether the RRD3 fragment has promoter activity in plants. Transgenic tobacco and rice calli both showed β-glucuronidase (GUS) activity, indicating that RRD3 can act as a promoter in both monocot and dicot plants. Based on the promoter characteristic of RRD3, we designed a plant universal binary vector, pCRiRRD3, which is suitable for performing researches on plant RNA interference. This vector has two multiple cloning sites to facilitate sense and antisense cloning of the target sequence, separated by an intron fragment of 200 bp. The efficiency of the vector for gene silencing was assayed by histochemical and quantitative fluorometric GUS assays in transgenic tobacco. These research results suggested that this plant RNAi vector pCRiRRD3 can effectively perform gene silencing researches on both monocot and dicot plants. 展开更多
关键词 RRD3 PROMOTER GUS histochemical assay quantitative fluorometric GUS assay RNA interference
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Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion 被引量:19
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作者 Guang Yan Chen Huang Jun Cao Ke-Jian Huang Tao Jiang Zheng-Jun Qiu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第30期3757-3766,共10页
AIM: To investigate RNA interference targeting signal transducer and activator of transcription-3 (STAT3) on invasion of human pancreatic cancer cells.METHODS: We constructed three plasmids of RNA interference tar... AIM: To investigate RNA interference targeting signal transducer and activator of transcription-3 (STAT3) on invasion of human pancreatic cancer cells.METHODS: We constructed three plasmids of RNA interference targeting the STAT3 gene. After LV (lentivirus)-STAT3siRNA (STAT3 small interfering RNA) the vector was transfected into the human pancreatic cell line, SW1990 and cell proliferation was measured by the MTT assay. Flow cytometry was used to assess cell cycle. Vascular endothelial growth favor (VEGF) and matrix metalloproteinase-2 (MMP-2) mRNA and protein expression were examined by quantitative PCR and western blotting, respectively. The invasion ability of SW1990 cells was determined by cell invasion assay.RESULTS: We successfully constructed the LVSTAT3siRNA lentivirus vector and proved that it can suppress expression of STAT3 gene in SW1990 cells. RNA interference of STAT3 by the LV-STAT3siRNA construct significantly inhibited the growth of SW1990 cells, in addition to significantly decreasing both VEGF and MMP-2 mRNA and protein expression. Moreover, suppression of STAT3 by LV-STAT3siRNA decreased the invasion ability of SW1990 cells.CONCLUSION: The STAT3 signaling pathway may provide a novel therapeutic target for the treatment of pancreatic cancer since it inhibits the invasion ability of pancreatic cancer cells. 展开更多
关键词 Signal transducer and activator of transcription3 RNA interference Lentivirus vector Pancreatic cancercells INVASION
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Lentivirus vectors construction of SiRNA targeting interference GPC3 gene and its biological effects on liver cancer cell lines Huh-7 被引量:8
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作者 Chang-Jiang Lei Chun Yao +5 位作者 Qing-Yun Pan Hao-Cheng Long Lei Li Shu-Ping Zheng Cheng Zeng Jian-Bin Huang 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2014年第10期780-786,共7页
Objective:To build GPC3 gene short hairpin interference RNA(shRNA)slow virus veclor.observe expression of Huh-7 GPC3 gene in human liver cell line proliferation apoptosis and the effect of GPC3 gene influencing on liv... Objective:To build GPC3 gene short hairpin interference RNA(shRNA)slow virus veclor.observe expression of Huh-7 GPC3 gene in human liver cell line proliferation apoptosis and the effect of GPC3 gene influencing on liver cancer cell growth,and provide theoretical basis for genc therapy of liver cancer.Methods:Hepatocellular carcinoma cell line Huh-7 wsa transfected by a RNA interference technique.GPC3 gene expression in a variety of liver cancer cell lines was detected by fluorescence quantitative PCR.Targeted GPC3 gene seqnences of small interfering RNA(siRNA)PGC-shRNA-GPC3 were restructured.Stable expression cell linse of siRNA were screened and established with the heplp of liposomes(lipofectamine^(TM2000))as carrier transfcetion of human liver cell lines.In order to validate siRNA interference efficiency.GPC3 siRNA mRNA expression was detected after transfection by using RT-PCR and Western blot.The absorbance value of the cells of blank group,untransfection group and transfection group,the cell cycle and cell apoptosis were calculated,and effects of GPC3 gene nn Huh-7 cell proliferation and apoptosis were observed.Results:In the liver cancer cell lines Huh-7 GPC3 gene showed high expression.PGC-shRNA-GPC3 recombinant plasmid was constructde successfully via sequencing validation.Stable recombinant plasmid transfected into liver cancer cell linse Huh-7can obviously inhibit GPC3 mRNA expression level.Conclusions:The targeted GPC3 siRNA can effectively inhibit the expression of GPC3. 展开更多
关键词 GPC3 GENE SLOW VIRUS CARRIER Liver cancer cell lines RNA interference
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Silencing of signal transducer and activator of transcription 3 expression by RNA interference suppresses growth of human hepatocellular carcinoma in tumor-bearing nude mice 被引量:13
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作者 Jing Li Yun-Feng Piao +2 位作者 Zheng Jiang Li Chen Hai-Bo Sun 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第21期2602-2608,共7页
AIM: To explore the effect of silencing of signal transducer and activator of transcription 3 (STAT3) expression by RNA interference (RNAi) on growth of human hepatocellular carcinoma (HCC) in tumorbearing nude... AIM: To explore the effect of silencing of signal transducer and activator of transcription 3 (STAT3) expression by RNA interference (RNAi) on growth of human hepatocellular carcinoma (HCC) in tumorbearing nude mice in vivo.METHODS: To construct the recombinant plasmid of pSilencer 3.0-H1-STAT3-siRNA-GFP (pSHI-siRNA- STAT3) and establish the tumor-bearing nude mouse model of the HCC cell line SMMC7721, we used intratumoral injection together with electroblotting to transfect the recombinant plasmid pSHI-siRNA- STAT3 into the transplanted tumor. The weight of the nude mice and tumor volumes were recorded. STAT3 gene transcription was detected by semi-quantitative reverse transcription polymerase chain reaction (RT- PCR). Level of protein expression and location of STAT3 were determined by Western blotting and immunohistochemical staining. STAT3-related genes such as survivin, c-myc, VEGF, p53 and caspase3 mRNA and protein expression were detected in tumor tissues at the same time. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was used to detect apoptosis of tumor cells.RESULTS: The weight of the treated nude mice increased, and the tumor volume decreased markedly compared with those of the mock-treated and negative control groups (P 〈 0.01). The results of RT-PCR and Western blotting showed that mRNA and protein levels of STAT3 declined markedly in the treated group. The change in STAT3-related gene expression in tumor tissues at the mRNA and protein level also varied, the expression of survivin, VEGF and c-myc were obviously reduced, and expression of p53 and caspase3 increased (P 〈 0.01). Most of the tumor tissue ceils in the treated group developed apoptosis that was detected by TUNEL assay.CONCLUSION: Silencing of STAT3 expression by RNAi significantly inhibits expression of STAT3 mRNA and protein, and suppresses growth of human HCC in tumor-bearing nude mice. The mechanism may be related to down-regulation of survivin, VEGF and c-myc and up-regulation of p53 and caspase3 expression. Accordingly, the STAT3 gene may act as an important and effective target in gene therapy of HCC. 展开更多
关键词 RNA interference Signal transducerand activator of transcription 3 transcription factor Hepatocellular carcinoma Xenograft model antitumorassays Nude mouse
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Phosphoinositide-3-kinase,catalytic,alpha polypeptide RNA interference inhibits growth of colon cancer cell SW948 被引量:4
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作者 Wen-Sheng Huang Tian-Bao Wang +3 位作者 Yao He Yu-Jun Chen Shi-Long Zhong Min Tan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第26期3458-3464,共7页
AIM:To investigate the gene knock-down effect by the phosphoinositide-3-kinase,catalytic,alpha polypeptide(PIK3CA)-targeted double-stranded RNA(dsRNA) and its effect on cell proliferation and cycle distribution in SW9... AIM:To investigate the gene knock-down effect by the phosphoinositide-3-kinase,catalytic,alpha polypeptide(PIK3CA)-targeted double-stranded RNA(dsRNA) and its effect on cell proliferation and cycle distribution in SW948.METHODS:Two PIK3CA-targeted dsRNAs were constructed and transfected into SW948 cells.Transfections were performed using lipofectamine TM 2000.The transfection effectiveness was calculated basing on the rate of fluorescence cell of SW948 at 6 h after transfection.Total messenger RNA was extracted from these cells using the RNeasy kit,and semiquantitative reverse transcription polymerase chain reaction was performed to detect the down-regulation of PIK3CA,AKT1,MYC,and CCND1 gene expression.Cells were harvested,proteins were resolved,and western blot was employed to detect the expression levels of PIK3CA,AKT1,MYC,and CCND1 gene.Cell proliferation was assessed by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay and the inhibition rate was calculated.Soft agar colony formation assay was performed basing on colonies greater than 60 μm in diameter at ×100 magnification.The effect on cell cycle distribution and apoptosis was assessed by flow cytometry.All experiments were performed in triplicate.RESULTS:Green fluorescence was observed in SW948 cell transfected with plasmid Pgenesil-1,and the transfection effectiveness was about 65%.Forty-eight hours post-transfection,mRNA expression of PIK3CA in SW948 cells was 0.51 ± 0.04 vs 0.49 ± 0.03 vs 0.92 ± 0.01 vs 0.93 ± 0.03(P = 0.001) in Pgenesil-CA1,Pgenesil-CA2,negative and blank group respectively.mRNA expression of AKT1 was 0.50 ± 0.03 vs 0.48 ± 0.01 vs 0.93 ± 0.04 vs 0.92 ± 0.02(P = 0.000) in Pgenesil-CA1,Pgenesil-CA2,negative and blank group respectively.mRNA expression of MYC was 0.49 ± 0.01 vs 0.50 ± 0.04 vs 0.90 ± 0.02 vs 0.91 ± 0.03(P = 0.001) in the four groups respectively.mRNA expression of CCND1 was 0.45 ± 0.02 vs 0.51 ± 0.01 vs 0.96 ± 0.03 vs 0.98 ± 0.01(P = 0.001) in the four groups respectively.The protein level of PIK3CA was 0.53 ± 0.01 vs 0.54 ± 0.02 vs 0.92 ± 0.03 vs 0.91 ± 0.02(P = 0.001) in Pgenesil-CA1,Pgenesil-CA2,negative and blank group respectively.The protein level of AKT1 in the four groups was 0.49 ± 0.02 vs 0.55 ± 0.03 vs 0.94 ± 0.03 vs 0.95 ± 0.04,P = 0.000).The protein level of MYC in the four groups was 0.51 ± 0.03 vs 0.52 ± 0.04 vs 0.92 ± 0.02 vs 0.95 ± 0.01(P = 0.000).The protein level of CCND1 in the four groups was 0.54 ± 0.04 vs 0.56 ± 0.03 vs 0.93 ± 0.01 vs 0.93 ± 0.03(P = 0.000).Both Pgenesil-CA1 and Pgenesil-CA2 plasmids significantly suppressed the growth of SW948 cells when compared with the negative or blank group at 48 h after transfec-tion(29% vs 25% vs 17% vs 14%,P = 0.001),60 h after transfection(38% vs 34% vs 19% vs 16%,P = 0.001),and 72 h after transfection(53% vs 48% vs 20% vs 17%,P = 0.000).Numbers of colonies in negative,blank,CA1,and CA2 groups were 42 ± 4,45 ± 5,8 ± 2,and 10 ± 3,respectively(P = 0.000).There were more than 4.5 times colonies in the blank and negative control groups as there were in the CA1 and CA2 groups.In addition,the colonies in blank and negative control groups were also larger than those in the CA1 and CA2 groups.The percentage of cells in the CA1 and CA2 groups was significantly higher in G 0 /G 1 phase,but lower in S and G 2 /M phase when compared with the negative and control groups.Moreover,cell apoptosis rates in the CA1 and CA2 groups were 5.11 ± 0.32 and 4.73 ± 0.32,which were significantly higher than those in negative(0.95 ± 0.11,P = 0.000) and blank groups(0.86 ± 0.13,P = 0.001).No significant difference was found between CA1 and CA2 groups in cell cycle distribution and apoptosis.CONCLUSION:PIK3CA-targeted short hairpin RNAs can block the phosphoinositide 3-kinase-Akt signaling pathway and inhibit cell growth,increase apoptosis,and induce cell cycle arrest in the PIK3CA-mutant colon cancer SW948 cells. 展开更多
关键词 Phosphoinositide-3-kinase catalytic alphapolypeptide RNA interference Colon cancer Phos-phoinositide-3-kinase pathway
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Mechanically strong and folding-endurance Ti_(3)C_(2)T_(x) MXene/PBO nanofiber films for efficient electromagnetic interference shielding and thermal management 被引量:16
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作者 Lei Wang Zhonglei Ma +3 位作者 Yali Zhang Hua Qiu Kunpeng Ruan Junwei Gu 《Carbon Energy》 SCIE CAS 2022年第2期200-210,共11页
Electromagnetic interference(EMI)shielding materials with excellent flexibility and mechanical properties and outstanding thermal conductivity have become a hot topic of research in functional composites.In this study... Electromagnetic interference(EMI)shielding materials with excellent flexibility and mechanical properties and outstanding thermal conductivity have become a hot topic of research in functional composites.In this study,the“sol-gel-film conversion technique”is used to assemble polyetherimidefunctionalized Ti_(3)C_(2)T_(x) nanosheets(f-Ti_(3)C_(2)T_(x))with poly(p-phenylene-2,6-benzobisoxazole)(PBO)nanofibers(PNFs),followed by dialysis and vacuum drying to prepare f-Ti_(3)C_(2)T_(x)/PNF films with lamellar structures.When the loading of f-Ti_(3)C_(2)T_(x) is 70 wt%,the f-Ti_(3)C_(2)T_(x)/PNF film presents optimal comprehensive properties,with an EMI shielding effectiveness(SE)of 35 dB and a specific SE/thickness((SSE,SE/density)/t)of 8211 dB cm^(2)/g,a tensile strength of 125.1 MPa,an in-plane thermal conductivity coefficient(λ)of 5.82 W/(m K),and electrical conductivity of 1943 S/m.After repeated folding for 10,000 cycles,the EMI SE and the tensile strength of f-Ti_(3)C_(2)T_(x)/PNFs films still remain 33.4 dB and 116.1 MPa,respectively.Additionally,the f-Ti_(3)C_(2)T_(x)/PNF film also shows excellent thermal stability,flame retardancy,and structural stability.This would provide a novel method for the design and fabrication of multifunctional composite films and considerably expand the applications of MXene-and PNF-based composites in the fields of EMI shielding and thermal management. 展开更多
关键词 electromagnetic interference shielding PBO nanofibers thermal management Ti_(3)C_(2)T_(x)MXene
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Effects of RNA Interference Combined with Ultrasonic Irradiation and SonoV ue Microbubbles on Expression of STAT3 Gene in Keratinocytes of Psoriatic Lesions 被引量:4
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作者 冉立伟 王昊 +2 位作者 兰东 贾红侠 于思思 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2017年第2期279-285,共7页
The most effective sequence of small interfering RNA(si RNA) silencing STAT3 of psoriatic keratinocytes(KCs) was screened out,and the effects of the most effective si RNA combined with ultrasonic irradiation and S... The most effective sequence of small interfering RNA(si RNA) silencing STAT3 of psoriatic keratinocytes(KCs) was screened out,and the effects of the most effective si RNA combined with ultrasonic irradiation and Sono Vue microbubbles on the expression of STAT3 of KCs and the dose-and time-response were investigated.Three chemically-synthetic si RNAs targeting STAT3 carried by Lipofectamine 3000 were transfected into KCs,and the effects on STAT3 expression were detected,then the most effective si RNA was selected for the subsequent experiments.The negative controls of siR NA(si RNA-NC) labeled with Cy3 carried by Lipofectamine 3000 combined with ultrasonic irradiation and Sono Vue microbubbles were transfected into KCs,then the optimal parameters of ultrasonic irradiation were determined.The most effective si RNA carried by Lipofectamine 3000 combined with ultrasonic irradiation at the optimal parameters and Sono Vue microbubbles was transfected into KCs,and the dose-and time-response of RNA interference was determined.The effect of RNA interference by the most effective si RNA at the optimal time and dose carried by Lipofectamine 3000 combined with ultrasonic irradiation and Sono Vue microbubbles(LUS group) was compared with that only carried by Lipofectamine 3000(L group).The results showed that si RNA-3 achieved the highest silencing efficacy.0.5 W/cm2 and 30 s were selected as the parameters of ultrasonic irradiation.The si RNA-3 carried by Lipofectamine 3000 combined with ultrasonic irradiation and Sono Vue microbubbles could effectively knock down the STAT3 expression at m RNA and protein levels in dose-and time-dependent manners determined at 100 nmol/L with maximum downregulation on m RNA at 48 h,and on protein at 72 h after transfection.The LUS group achieved the highest silencing efficacy.It was concluded that si RNA-3 carried by Lipofectamine 3000 combined with ultrasonic irradiation and SonoV ue microbubbles could effectively knock down the STAT3 expression in psoriatic KCs,and the optimized transfection condition and the sequence of si RNA-3 could serve for further research on gene therapy of psoriasis. 展开更多
关键词 STAT3 keratinocytes psoriasis RNA interference small interfering RNA ultrasonic irradiation microbubbles expression
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涂覆SnO_(2)-WO_(3)的微光纤甲烷气体传感器
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作者 陈苗 傅海威 +3 位作者 肖家悦 李根 程嘉慧 丁继军 《光电技术应用》 2024年第2期37-43,共7页
提出并展示了一种用于甲烷浓度检测的SnO_(2)-WO_(3)纳米花涂覆微光纤双锥干涉仪(MFI)。采用多芯光纤熔融双锥法制备锥形MFI。采用水热法和浸渍法合成了甲烷敏感材料SnO_(2)-WO_(3)。采用滴涂法在MFI结构外部涂覆SnO_(2)-WO_(3)材料。... 提出并展示了一种用于甲烷浓度检测的SnO_(2)-WO_(3)纳米花涂覆微光纤双锥干涉仪(MFI)。采用多芯光纤熔融双锥法制备锥形MFI。采用水热法和浸渍法合成了甲烷敏感材料SnO_(2)-WO_(3)。采用滴涂法在MFI结构外部涂覆SnO_(2)-WO_(3)材料。当甲烷分子吸附在涂层材料上时,材料的折射率会发生变化,从而引起传感器的透射光谱发生变化。因此,可以通过测量光谱的波长漂移来检测甲烷浓度。实验结果表明,SnO_(2)-WO_(3)纳米花包覆MFI传感器具有较大的浓度检测范围和较高的室温稳定性。测量甲烷浓度范围为0~34%,灵敏度约为0.11nm/%。还对传感器的选择性进行了研究。对异丙醇、甲醇、乙醇和甲烷的反应进行了研究和比较。结果表明,该传感器对甲烷具有较高的吸附选择性,在矿井瓦斯检测与预警领域具有潜在的应用前景。 展开更多
关键词 甲烷气体传感 SnO_(2)-WO_(3)纳米材料 微光纤 干涉
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Investigating the Genetic Bases of Growth Regulation by E2F3 in Dwarf Surf Clams Mulinia lateralis
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作者 YAO Ruixing WANG Chen +3 位作者 KONG Lingling WANG Yujue BAO Zhenmin HU Xiaoli 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第5期1347-1358,共12页
Bivalve aquaculture plays a crucial role in the aquaculture industry due to the economic value of many bivalve species.Understanding the underlying genetic basis of bivalve growth regulation is essential for enhancing... Bivalve aquaculture plays a crucial role in the aquaculture industry due to the economic value of many bivalve species.Understanding the underlying genetic basis of bivalve growth regulation is essential for enhancing germplasm innovation and ensuring sustainable development of the industry.Though numerous candidate genes have been identified,their functional validation remains challenging.Fortunately,the dwarf surf clam(Mulinia lateralis)serves as a promising model organism for investigating genetic mechanisms underlying growth regulation in bivalves.The GWAS study in the Yesso scallop(Patinopecten yessoensis)has pinpointed the E2F3 gene as a key regulator of growth-related traits.However,the specific role of E2F3 in bivalve growth remains unclear.This study aimed to further confirm the regulatory function of the E2F3 gene in the dwarf surf clam through RNA interference experiments.Our results revealed several genes are associated with individual growth and development,including CTS7,HSP70B2,and PGLYRP3,as well as genes involved in lipid metabolism such as FABP2 and FASN.Functional enrichment analysis indicated that E2F3 primarily modulates critical processes like amino acid and lipid metabolism.These findings suggest that E2F3 likely regulates growth in the dwarf surf clam by influencing amino acid and lipid metabolism.Overall,this study advances our understanding on the function of E2F3 gene in growth regulation in bivalves,providing valuable insights for future research in this field. 展开更多
关键词 growth regulation E2F3 RNA interference experiment transcriptomic analysis dwarf surf clam Mulinia lateralis BIVALVE
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Self-Interference Elimination by Physical Feedback Channel in CCFD for 3-D Beamforming Communication 被引量:1
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作者 Naveed Ali Kaimkhani Zhe Chen Fuliang Yin 《China Communications》 SCIE CSCD 2017年第9期62-71,共10页
The 3-D beamforming scheme has elite as evolving interest because of its efficiency to empower assorted techniques such as vertical and horizontal domains and emanation beamforming according to subscriber's provis... The 3-D beamforming scheme has elite as evolving interest because of its efficiency to empower assorted techniques such as vertical and horizontal domains and emanation beamforming according to subscriber's provisions. Usually, 3-D beamforming communication is set up on FDD/TDD approach those effects on the performance of spectrum and energy efficiency. Co-frequency and CoTime Full Duplex(CCFD) is an effective solution to improve the spectrum and energy efficiency by transmitting and receiving simultaneously in frequency and time domain. While, CCFD communication often face the self-interference issue when communication occurs, simultaneously. Consequently, in this paper a self-interference elimination by physical feedback channel in CCFD for 3-D Beamforming communication scheme is proposed to improve the over-all system performance in terms of energy and spectrum efficiency. The simulation and analytical outcomes demonstrated that the proposed system is superior than the traditional one. 展开更多
关键词 spectrum efficiency energy effi-ciency co-frequency and co-time full DUPLEX SELF-interference suppression 3-D beamform-ing
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基于第一性原理的无铅无机钙钛矿Cs_(3)Bi_(2)X_(9)(X=Cl、Br、I)光电性能表面效应研究
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作者 彭程 韦建卫 +2 位作者 陈亚婷 胡南 曾晖 《无机化学学报》 SCIE CAS CSCD 北大核心 2024年第3期555-560,共6页
用基于第一性原理的密度泛函理论方法,对Cs_(3)Bi_(2)X_(9)(X=Cl、Br、I)的光电特性进行理论计算,并系统阐述这3种晶体的表面效应对光电性能的影响。结果表明,3种材料的光学特性由铋原子和卤素原子最外层p轨道上的价电子主导。在可见光... 用基于第一性原理的密度泛函理论方法,对Cs_(3)Bi_(2)X_(9)(X=Cl、Br、I)的光电特性进行理论计算,并系统阐述这3种晶体的表面效应对光电性能的影响。结果表明,3种材料的光学特性由铋原子和卤素原子最外层p轨道上的价电子主导。在可见光区中,材料的吸收峰会随卤素原子序数的增加呈现红移,其中一维结构的Cs_(3)Bi_(2)Cl_(9)表面结构在光吸收能力上尤为特别且敏感;二维结构的Cs_(3)Bi_(2)Br_(9)光吸收能力会受厚度影响;零维结构的Cs_(3)Bi_(2)I_(9)非常稳定,且几乎不受表面特性和晶体厚度的影响。 展开更多
关键词 表面效应 Cs_(3)Bi_(2)X_(9) 钙钛矿 第一性原理
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GATA-3小干扰RNA载体对变应性鼻炎小鼠外周血单个核细胞中辅助性T细胞亚群功能的体内调节机制 被引量:1
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作者 李玉晓 贺铭 +1 位作者 王天蓉 王雨 《中国耳鼻咽喉头颈外科》 CSCD 2023年第10期646-651,共6页
目的 研究RNA干扰(RNA interference,RNAi)介导的GATA-3小干扰RNA载体(si-GATA-3)对变应性鼻炎(AR)小鼠外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中辅助性T细胞1(T-helper 1,Th1)和Th2功能的调节作用。方法 构建si-G... 目的 研究RNA干扰(RNA interference,RNAi)介导的GATA-3小干扰RNA载体(si-GATA-3)对变应性鼻炎(AR)小鼠外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中辅助性T细胞1(T-helper 1,Th1)和Th2功能的调节作用。方法 构建si-GATA-3,用卵清蛋白构建BALB/c AR模型,si-GATA-3组、si-NC组(无目标基因小干扰RNA的空白对照组)分别用si-GATA-3和si-NC腹腔注射干预AR小鼠,AR组和正常对照组用等量的生理盐水干预。干预结束抽取其外周血,分离PBMCs和血浆。分别用荧光定量PCR和Western blot技术检测PBMCs中GATA-3和T-bet mRNA及其相应蛋白的表达量;用ELISA技术检测血浆中白细胞介素4(IL-4)和干扰素γ(IFN-γ)的含量。结果 si-GATA-3滴度为5×10^(8)TU/ml。成功制备BALB/C小鼠AR模型。si-GATA-3能明显改善AR小鼠的症状。si-GATA-3组PBMCs中GATA-3 mRNA表达明显低于AR组和si-NC组(P<0.01),AR组和si-NC组明显高于正常对照组(P<0.01)。PBMCs中GATA-3蛋白和血浆中IL-4的表达量与GATA-3 mRNA表达相似。si-GATA-3组PBMCs中T-bet mRNA及其蛋白的相对表达均明显高于AR组和si-NC组(P<0.01),AR组和si-NC组二者均明显低于对照组(P<0.01)。si-GATA-3组血浆中IFN-γ的表达量高于正常对照组(P<0.05);AR组与si-NC组中IFN-γ的表达量均明显高于正常对照组和si-GATA-3组(P<0.01)。结论 si-GATA-3能有效改善AR小鼠症状,下调AR小鼠PBMCs中Th2细胞中GATA-3 mRNA、GATA-3蛋白和IL-4的表达,同时上调Th1细胞中T-bet mRNA和T-bet蛋白的表达,纠正Th2/Th1的免疫失衡。 展开更多
关键词 鼻炎 过敏 RNA干扰 模型 动物 小鼠 GATA-3 T-BET 慢病毒载体
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Effects of small interfering RNA inhibit Class Ⅰ phosphoinositide 3-kinase on human gastric cancer cells 被引量:8
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作者 Bao-Song Zhu Li-Yan Yu +7 位作者 Kui Zhao Yong-You Wu Xiao-Li Cheng Yong Wu Feng-Yun Zhong Wei Gong Qiang Chen Chun-Gen Xing 《World Journal of Gastroenterology》 SCIE CAS 2013年第11期1760-1769,共10页
AIM: To investigate the effects of small interfering RNA (siRNA)-mediated inhibition of Class?I?phosphoinositide 3-kinase (Class?I?PI3K) signal transduction on the proliferation, apoptosis, and autophagy of gastric ca... AIM: To investigate the effects of small interfering RNA (siRNA)-mediated inhibition of Class?I?phosphoinositide 3-kinase (Class?I?PI3K) signal transduction on the proliferation, apoptosis, and autophagy of gastric cancer SGC7901 and MGC803 cells.METHODS: We constructed the recombinant replication adenovirus PI3K(I)-RNA interference (RNAi)-green fluorescent protein (GFP) and control adenovirus NC-RNAi-GFP, and infected it into human gastric cancer cells. MTT assay was used to determine the growth rate of the gastric cancer cells. Activation of autophagy was monitored with monodansylcadaverine (MDC) staining after adenovirus PI3K(I)-RNAi-GFP and control adenovirus NC-RNAi-GFP treatment. Immunofluorescence staining was used to detect the expression of microtubule-associated protein 1 light chain 3 (LC3). Mitochondrial membrane potential was measured using the fluorescent probe JC-1. The expression of autophagy was monitored with MDC, LC3 staining, and transmission electron microscopy. Western blotting was used to detect p53, Beclin-1, Bcl-2, and LC3 protein expression in the culture supernatant.RESULTS: The viability of gastric cancer cells was inhibited after siRNA targeting to the Class?I?PI3K blocked Class?I?PI3K signal pathway. MTT assays revealed that, after SGC7901 cancer cells were treated with adenovirus PI3K(I)-RNAi-GFP, the rate of inhibition reached 27.48% ± 2.71% at 24 h, 41.92% ± 2.02% at 48 h, and 50.85% ± 0.91% at 72 h. After MGC803 cancer cells were treated with adenovirus PI3K(I)-RNAi-GFP, the rate of inhibition reached 24.39% ± 0.93% at 24 h, 47.00% ± 0.87% at 48 h, and 70.30% ± 0.86% at 72 h (P < 0.05 compared to control group). It was determined that when 50 MOI, the transfection efficiency was 95% ± 2.4%. Adenovirus PI3K(I)-RNAi-GFP (50 MOI) induced mitochondrial dysfunction and activated cell apoptosis in SGC7901 cells, and the results described here prove that RNAi of Class?I?PI3K induced apoptosis in SGC7901 cells. The results showed that adenovirus PI3K(I)-RNAi-GFP transfection induced punctate distribution of LC3 immunoreactivity, indicating increased formation of autophagosomes. The results showed that the basal level of Beclin-1 and LC3 protein in SGC7901 cells was low. After incubating with adenovirus PI3K(I)-RNAi-GFP (50 MOI), Beclin-1, LC3, and p53 protein expression was significantly increased from 24 to 72 h. We also found that Bcl-2 protein expression down-regulated with the treatment of adenovirus PI3K(I)-RNAi-GFP (50 MOI). A number of isolated membranes, possibly derived from ribosome-free endoplasmic reticulum, were seen. These isolated membranes were elongated and curved to engulf a cytoplasmic fraction and organelles. We used transmission electron microscopy to identify ultrastructural changes in SGC7901 cells after adenovirus PI3K(I)-RNAi-GFP (50 MOI) treatment. Control cells showed a round shape and contained normal-looking organelles, nucleus, and chromatin, while adenovirus PI3K(I)-RNAi-GFP (50 MOI)-treated cells exhibited the typical signs of autophagy.CONCLUSION: After the Class?I?PI3K signaling pathway has been blocked by siRNA, the proliferation of cells was inhibited and the apoptosis of gastric cancer cells was enhanced. 展开更多
关键词 Gastric cancer cells Class I phosphoinositide 3-kinase RNA interference Apoptosis Autophagy
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Down-regulation of STAT3 expression by vector-based small interfering RNA inhibits pancreatic cancer growth 被引量:6
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作者 Chen Huang Guang Yang +3 位作者 Tao Jiang Jun Cao Ke-Jian Huang Zheng-Jun Qiu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第25期2992-3001,共10页
AIM:To evaluate the effect of RNA interference (RNAi) mediated silence of signal transduction and activation of transcription (STAT)3 on the growth of human pancreatic cancer cells both in vitro and in vivo.METHODS:ST... AIM:To evaluate the effect of RNA interference (RNAi) mediated silence of signal transduction and activation of transcription (STAT)3 on the growth of human pancreatic cancer cells both in vitro and in vivo.METHODS:STAT3 specific shRNA was used to silence the expression of STAT3 in pancreatic cancer cell line SW1990.The anti-growth effects of RNAi against STAT3 were studied in vitro and in experimental cancer xenografts in nude mice.The potential pathways involved in STAT3 signaling were detected using reverse transcription polymerase chain reaction and western blotting.RESULTS:The expression of the STAT3 was inhibited using RNAi in SW1990 cells.RNAi against STAT3 inhibited cell proliferation,induced cell apoptosis and significantly reduced the levels of CyclinD1 and Bcl-xL when compared with parental and control vector-transfected cells.In vivo experiments showed that RNAi against STAT3 inhibited the tumorigenicity of SW1990 cells and significantly suppressed tumor growth when it was directly injected into tumors.CONCLUSION:STAT3 signaling pathway plays an important role in the progression of pancreatic cancer,and silence of STAT3 gene using RNAi technique may be a novel therapeutic option for treatment of pancreatic cancer. 展开更多
关键词 Signal transduction and activation of tran-scription 3 RNA interference Pancreatic cancer GROWTH
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Significantly Enhanced Electromagnetic Interference Shielding Performances of Epoxy Nanocomposites with Long-Range Aligned Lamellar Structures 被引量:11
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作者 Lei Wang Zhonglei Ma +3 位作者 Hua Qiu Yali Zhang Ze Yu Junwei Gu 《Nano-Micro Letters》 SCIE EI CAS CSCD 2022年第12期617-629,共13页
High-efficiency electromagnetic interference(EMI)shielding materials are of great importance for electronic equipment reliability,information security and human health.In this work,bidirectional aligned Ti_(3)C_(2)T_(... High-efficiency electromagnetic interference(EMI)shielding materials are of great importance for electronic equipment reliability,information security and human health.In this work,bidirectional aligned Ti_(3)C_(2)T_(x)@Fe_(3)O_(4)/CNF aerogels(BTFCA)were firstly assembled by bidirectional freezing and freeze-drying technique,and the BTFCA/epoxy nanocomposites with long-range aligned lamellar structures were then prepared by vacuum-assisted impregnation of epoxy resins.Benefitting from the successful construction of bidirectional aligned three-dimensional conductive networks and electromagnetic synergistic effect,when the mass fraction of Ti_(3)C_(2)T_(x) and Fe_(3)O_(4) are 2.96 and 1.48 wt%,BTFCA/epoxy nanocomposites show outstanding EMI shield-ing effectiveness of 79 dB,about 10 times of that of blended Ti_(3)C_(2)T_(x)@Fe_(3)O_(4)/epoxy(8 dB)nanocomposites with the same loadings of Ti_(3)C_(2)T_(x) and Fe_(3)O_(4).Meantime,the corresponding BTFCA/epoxy nanocomposites also present excellent thermal stability(T_(heat-resistance index) of 198.7℃)and mechanical properties(storage modulus of 9902.1 MPa,Young’s modulus of 4.51 GPa and hardness of 0.34 GPa).Our fabricated BTFCA/epoxy nanocomposites would greatly expand the applications of MXene and epoxy resins in the fields of information security,aerospace and weapon manufacturing,etc. 展开更多
关键词 Electromagnetic interference shielding Epoxy nanocomposites Ti_(3)C_(2)Tx Fe_(3)O_(4) Bidirectional aligned three-dimensional conductive networks
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PIM Interference Analysis under Multi-band Multi-signal Input in Duplex Indoor Distribution System 被引量:2
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作者 Pauling Huang 《China Communications》 SCIE CSCD 2007年第3期79-87,共9页
This analysis focuses on PIM interference under multi-band multi-signal input in mobile communication system.Unlike single band system that only odd order PIM(especially 3rd order) should be concerned,in multi-band mu... This analysis focuses on PIM interference under multi-band multi-signal input in mobile communication system.Unlike single band system that only odd order PIM(especially 3rd order) should be concerned,in multi-band multi-signal case,both odd and even order PIM could be interference source because of more complicated intermodulation,more IMPs generated and more receive bands.Especially,the 2nd order PIM may interfere more serious to receiving channel for its strong magnitude.In duplex indoor distribute system,the PIM interference is a potential problem to GSM900,DCS1800,CDMA,and even 3G system wireless services,because the PIM frequencies may fall in receive bands and interfere to the receiving channels.In radio system design and wireless channel assignment,precautions must be taken to minimize the PIM level and to avoid interfere to receiving channels.For practical use,the lower order possible PIM interference to 2G and 3G systems is calculated. 展开更多
关键词 PIM IMP interference MULTI-BAND multi-signal 2G and 3G systems
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Interference Coordination for FD-MIMO Cellular Network with D2D Communications Underlaying 被引量:2
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作者 Xiao Li Nana Qin Tingting Sun 《China Communications》 SCIE CSCD 2018年第12期75-88,共14页
In this paper, we investigate the interference coordination for downlink full-dimension multiple-input multiple-output(FD-MIMO) systems with device-to-device(D2 D) communications underlaying. With three-dimensional(3 ... In this paper, we investigate the interference coordination for downlink full-dimension multiple-input multiple-output(FD-MIMO) systems with device-to-device(D2 D) communications underlaying. With three-dimensional(3 D) beamforming transmission applied for cellular users(CUEs), an approximation of the interference to signal ratio for CUEs is derived, and a coordination strategy is proposed to mitigate the interference from D2 D pairs to CUEs. Based on the lower bound of the interference to signal ratio for D2 D pairs, we propose coordination strategies for D2 D pairs to mitigate the interference caused by base station(BS) and the interference between D2 D pairs. The proposed strategies require only some statistical channel state information(CSI) of each user and the reduced-dimensional effective CSI of a few CUEs and D2 D pairs. Simulation results show that the proposed coordination strategy performs well in terms of achieving good tradeoff between the achievable rate of CUEs and D2 D pairs. 展开更多
关键词 D2D FD-MIMO 3D BEAMFORMING interference COORDINATION
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