A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Modified Z-Plasty for Reconstruction of Webbed Scar Contractures

Rationale: Webbed scar contractures deformity caused by burns and other factors will lead to joint disorders and affect the mental health of patients, resulting in a severe decline in quality of life. Rapid, effective and less complicated surgical methods can help patients with post-burn rehabilitation. Objective: This article argues that a modified Z-plasty can quickly improve the range of motion caused by webbed scar contractures in joint areas, including surgical methods, postoperative care and prognosis. Methods and Results: The study took place from 2018 to 2022. Thirty-two patients with joint scar contracture deformity, with a mean age of 32.5 years, were included in the study. All patients underwent contracture scar revision and modified Z-plasty repair under anesthesia. All the flaps survived and the joint function was improved. Compared with the traditional Z-plasty, the duration of the operative procedure of the modified Z-plasty was significantly shorter, more surrounding scar tissue was mobilized, and the effectiveness of postoperative scar contracture release was better. Discussions: The modified Z-plasty for scar contracture deformity in joint area is simple, rapid, effective and easy to manage.

Evaluation of modifi ed Alvarado scoring system and RIPASA scoring system as diagnostic tools of acute appendicitis

BACKGROUND: Acute appendicitis is the most common surgical condition presented in emergency departments worldwide. Clinical scoring systems, such as the Alvarado and modified Alvarado scoring systems, were developed with the goal of reducing the negative appendectomy rate to 5%–10%. The Raja Isteri Pengiran Anak Saleha Appendicitis(RIPASA) scoring system was established in 2008 specif ically for Asian populations. The aim of this study was to compare the modif ied Alvarado with the RIPASA scoring system in Kuwait population.METHODS: This study included 180 patients who underwent appendectomies and were documented as having "acute appendicitis" or "abdominal pain" in the operating theatre logbook(unit B) from November 2014 to March 2016. The sensitivity, specificity, positive predictive value(PPV), negative predictive value(NPV), diagnostic accuracy, predicted negative appendectomy and receiver operating characteristic(ROC) curve of the modified Alvarado and RIPASA scoring systems were derived using SPSS statistical software.RESULTS: A total of 136 patients were included in this study according to our criteria. The cut-off threshold point of the modif ied Alvarado score was set at 7.0, which yielded a sensitivity of 82.8% and a specif icity of 56%. The PPV was 89.3% and the NPV was 42.4%. The cut-off threshold point of the RIPASA score was set at 7.5, which yielded a 94.5% sensitivity and an 88% specif icity. The PPV was 97.2% and the NPV was 78.5%. The predicted negative appendectomy rates were 10.7% and 2.2% for the modif ied Alvarado and RIPASA scoring systems, respectively. The negative appendectomy rate decreased significantly, from 18.4% to 10.7% for the modified Alvarado, and to 2.2% for the RIPASA scoring system, which was a signif icant difference(P<0.001) for both scoring systems.CONCLUSION: Based on the results of this study, the RIPASA score is a simple scoring system with better sensitivity and specif icity than the modif ied Alvarado scoring system in Asian populations. It consists of 14 clinical parameters that can be obtained from a good patient history, clinical examination and laboratory investigations. The RIPASA scoring system is more accurate and specific than the modif ied Alvarado scoring system for Kuwait population.

Quantitative Analysis on Distribution of Modifier in Microstructure of SBS Modifi ed Asphalt

A method was developed to make a quantitative analysis of the microstructure of asphalt modified with styrene-butadiene-styrene block（SBS） copolymer. Moreover, both mixed and branched SBS were employed in the preparation of modified asphalt. By sampling specimens from the top, middle, and bottom, a number of microscope slides were made and 400 times micro images were gained. Binarized with the software of MATLAB, the images then went to the process of distribution test, when four typical distributions were employed to investigate the distribution of modifi er spread in asphalt. The distribution characteristic and its parameter were both found. The results show that asphalt phase clearly obeys Poisson distribution, and its parameter, λ, is bond up with the condition SBS performs in. Based on this parameter, indexes can be set up to evaluate the structure of the mixture and its performance as well. Compared with that modifi ed with mixed SBS, the asphalt modifi ed with branched SBS has better performance but worse stability, for it is easier to segregate.

Denitration Performance of Modifi ed AC Loaded with Nano V_2O_5 with Different Morphologies

A series of V2O5 with various morphologies was prepared by the hydrothermal method and loaded on the modified active coke（AC） which was prepared by the impregnation methods. The prepared samples were characterized by BET, Boehm titration test, XRD, SEM and EDS. The SEM exhibited that the morphologies of the samples prepared were signifi cantly different from each other. Then the samples prepared were studied on the selective catalytic reduction（SCR） of NO with ammonia in the presence of oxygen. The SCR activity measurements were undertaken in a fixed-bed unit with a sieve plate in the middle. From the contrastive experiments, the results showed that linear V2O5/AC had the best denitration performance and the denitration rate was up to 57.41%. It was speculated that the linear V2O5 with the crystal faces（110） may show the best performance in SCR. And the durability results also showed that linear V2O5/AC produced a denitration rate of 47.7% after three regenerations.

加味海龙黄精饮联合来曲唑治疗脾肾两虚型高雌二醇合并ED的临床研究

目的探究加味海龙黄精饮联合来曲唑治疗脾肾两虚型高雌二醇合并阴茎勃起功能障碍(erectile dysfunction,ED)的疗效。方法选择2016年1月至2018年5月大连大学附属中山医院中医科诊治的脾肾两虚型高雌二醇合并ED的70例患者,将其随机平均分为治疗组和对照组,每组35例患者。治疗组患者采用加味海龙黄精饮联合来曲唑治疗方法,对照组患者采用来曲唑治疗方法。治疗结束后去除失访者,实际完成治疗的治疗组有29例患者,对照组有29例患者,对两组患者治疗结果进行统计学分析。结果采用国际勃起功能指数(international index of erectile function,IIEF-5)评分,治疗后对照组患者病情无明显改善(P>0.05),治疗组患者评分[23(21,24)分]明显优于对照组患者[5(5,10)分],两者差异具有统计学意义(P<0.05)。血清雌二醇(estradiol,E2)含量,治疗后两组患者均有所降低,且治疗组患者E2含量[(28.45±8.94)pg/mL]明显优于对照组患者[(40.93±9.79)pg/mL],两者差异具有统计学意义(P<0.05)。血清睾酮(testosterone,T)水平,治疗后两组患者均有所提高,且治疗组患者水平[(21.20±5.50)nmol/L]与对照组患者水平[19.37(13.98,22.60)nmol/L]比较,其差异无统计学意义(P>0.05)。促性腺激素(follicule-stimulating hormone,FSH)和(luteinizing hormone,LH)的含量,治疗后两组患者均有所提高,且治疗组患者FSH[(14.59(8.19,18.88)mIU/mL]、LH[11.96(6.39,16.46)mIU/mL]明显优于对照组患者FSH[6.01(4.21,9.31)mIU/mL]、LH[5.38(3.86,9.30)mIU/mL],其差异均具有统计学意义(均P<0.05)。血清泌乳素(prolactin,PRL)含量,两组患者治疗前后比较,其差异均无统计学意义(均P>0.05)。结论加味海龙黄精饮联合来曲唑治疗脾肾两虚型高雌二醇合并阴茎勃起功能障碍的疗效显著,不仅能够降低E2、提高T水平,而且能够提高性欲。

Spectra of seismic force reduction factors of MDOF systems normalized by two characteristic periods

Seismic force reduction factor(SFRF) spectra of shear-type multi-degree-of-freedom(MDOF) structures are investigated. The modified Clough model, capable of considering the strength-degradation/hardening and stiffnessdegradation, is adopted. The SFRF mean spectra using 102 earthquake records on a typical site soil type(type C) are constructed with the period abscissa being divided into three period ranges to maintain the peak features at the two sitespecific characteristic periods. Based on a large number of results, it is found that the peak value of SFRF spectra may also exist for MDOF, induced by large high-mode contributions to elastic base shear, besides the mentioned two peak values. The variations of the stiffness ratio λk and the strength ratio λF of the top to bottom story are both considered. It is found that the SFRFs for λF ≤λk are smaller than those for λF > λk. A SFRF modification factor for MDOF systems is proposed with respect to SDOF. It is found that this factor is significantly affected by the story number and ductility. With a specific λF(= λk0.75), SFRF mean spectra are constructed and simple solutions are presented for MDOF systems. For frames satisfying the strong column/weak beam requirement, an approximate treatment in the MDOF shear-beam model is to assign a post-limit stiffness 15%-35% of the initial stiffness to the hysteretic curve. SFRF spectra for MDOF systems with 0.2 and 0.3 times the post-limit stiffness are remarkably larger than those without post-limit stiffness. Thus, the findings that frames with beam hinges have smaller ductility demand are explained through the large post-limit stiffness.

Spatio-temporal Pattern and Spatial Heterogeneity of Ecotones Based on Land Use Types of Southeastern Da Hinggan Mountains in China

Ecotones have received great attention due to its critical function in energy flux, species harbor, global carbon sequestration, and land-atmosphere interaction. This study investigated land use pattern and spatial heterogeneity of the ecotones among agricultural land, forest land, and grassland of the southeastern Da Hinggan Mountains in the northeastern China. The change of these delineated ecotones under different slopes and aridity conditions was analyzed by two landscape indices, edge density(ED) and core area percentage of landscape(CPL), to explore the inter-linkage between spatial structure of ecotones and socioeconomic development and land management. Specifically, the ecotones such as agriculture-forest(AF) ecotone, forest-grassland(FG) ecotone, and agriculture-forestgrassland(AFG) ecotone moved from the arid southeast to the humid northwest. The flat area with small slope is more edge-fragmented than the steep area since the ED decreases as the slope increases. The AF ecotone mostly found in the humid region is moving to more humid areas while the agriculture-grassland(AG) ecotone mostly found in the dry region is moving towards the drier region.

乳腺癌改良根治术后患者患侧肢体功能障碍的影响因素及与自我形象的关系

目的 探讨乳腺癌改良根治术后患者患侧肢体功能的影响因素及其与自我形象的关系,为促进该类患者患侧肢体功能恢复提供参考依据。方法 选择江西省肿瘤医院2020年8月—2022年8月行乳腺癌改良根治术治疗的乳腺癌患者126例为调查对象,调查患者基线资料及术后3~7 d乳腺癌自我形象量表(BIBCQ)评分;给予患者常规康复训练干预12周,训练结束后应用中文版上肢功能评定量表(DASH)简式评分表评价患侧肢体功能恢复情况,采用多元线性回归分析乳腺癌改良根治术后患者患侧肢体功能的影响因素,采用Pearson相关性分析乳腺癌改良根治术后患者患侧肢体功能与自我形象的关系。结果本研究共发放132份问卷,回收有效问卷126份。参与调查的126例乳腺癌改良根治术后患者DASH平均得分为26.48±4.06分。不同受教育程度、家庭功能患者DASH评分比较,差异有统计学意义(P<0.05)。126例乳腺癌改良根治术后患者BIBCQ量表平均得分为108.74±12.48分。经Pearson相关性分析显示,乳腺癌患者改良根治术后BIBCQ量表各维度评分与DASH评分均呈正相关(P<0.05)。经多元线性回归分析显示,文化程度低、家庭功能差及BIBCQ评分高是乳腺癌患者改良根治术后患侧肢体功能恢复的影响因素(P<0.05)。结论 乳腺癌改良根治术后患者患侧肢体功能恢复较差,自我形象评分高、初中及以下文化程度、家庭功能差均是影响患侧肢体功能恢复的重要因素。

改良Valsalva动作与小腿肌紧张动作在经胸右心声学造影诊断卵圆孔未闭中的应用价值

目的比较改良Valsalva动作(mVM)与小腿肌紧张动作在经胸右心声学造影(cTTE)诊断卵圆孔未闭(PFO)中的应用价值。方法选取2022年6月至2023年6月南京医科大学附属南京医院收治的87例疑似PFO患者作为研究对象。所有患者均接受经食管超声心动图(TEE)联合cTTE检查,判断右向左分流(RLS)来源,根据左心内是否出现微气泡及微气泡初始出现时间分为PFO组(存在异常血流)和正常组,比较分析两组的临床资料。所有患者分别在安静状态、mVM和小腿肌紧张动作下进行cTTE,比较分析这三种辅助状态下cTTE的PFO检出率、RLS微气泡初始出现的心动周期、RLS分流量检出程度。结果以TEE联合cTTE检查结果作为诊断的金标准,将87例患者分为PFO组68例(包括23例少量RLS、7例中量RLS、38例大量RLS)和正常组19例。两组临床资料比较显示,PFO组年龄小于正常组,出现头痛症状的比例高于正常组,差异均有统计学意义(P<0.05)。与安静状态相比,mVM和小腿肌紧张动作下的PFO检出率更高、RLS微气泡初始出现的心动周期更早,差异均有统计学意义(P<0.05),而mVM与小腿肌紧张动作下的PFO检出率、RLS微气泡初始出现的心动周期比较差异均无统计学意义(P>0.05)。安静状态、mVM、小腿肌紧张动作下少量RLS的检出率比较差异有统计学意义(P<0.05),而中量和大量RLS的检出率比较差异无统计学意义(P>0.05)。一致性检验结果显示,安静状态、mVM、小腿肌紧张动作下的PFO检出的特异度比较差异无统计学意义(P>0.05),但mVM、小腿肌紧张动作下的PFO检出的敏感度和Kappa值均显著高于安静状态(P<0.05),且以mVM的Kappa值最大,PFO检出结果与实际结果一致性最强。结论mVM与小腿肌紧张动作是cTTE诊断PFO的有效辅助方式,尤其是mVM在辅助cTTE诊断中的临床实际意义最强。小腿肌紧张动作是一种简单有效的辅助动作,当患者不能进行有效的mVM时,可采用小腿肌紧张动作进行替代。

乳腺癌改良根治术后延续护理的研究进展

乳腺癌已成为女性最常见的恶性肿瘤之一,其发病率呈逐年上升和年轻化趋势。改良根治术是乳腺癌常用的手术方法,延续护理可提高乳腺癌改良根治术后患者生活质量和护理满意度,降低医疗服务成本和肿瘤复发率等。本文综述了延续护理的概念,乳腺癌改良根治术后患者的延续护理阶段、内容及方法,旨在为乳腺癌改良根治术后延续护理的实践、研究提供理论依据。

改良注水法在女性患者无痛结肠镜检查中的应用研究

目的探讨改良注水法在女性患者无痛结肠镜检查中的应用价值。方法选择2022年11月—2024年4月因腹部症状就诊于烟台业达医院拟行单人无痛结肠镜检查的女性患者200例作为研究对象,以随机数表法将其分为注气组和改良注水组,每组100例,并记录年龄、身体质量指数(body mass index,BMI)、波士顿肠道准备评分(Boston Bowel Preparation Scale,BBPS)、既往盆腹腔手术史、便秘情况、达盲时间、退镜时间、息肉检出情况、肠道黏膜擦伤情况、结肠镜检查后腹部不适情况,比较两组息肉检出率、肠道黏膜擦伤情况、检查后腹部不适情况。结果改良注水组在无痛结肠镜检查中达盲时间明显低于注气组,差异有统计学意义(P<0.05),两组发现息肉情况、肠道黏粘膜擦伤情况、检查后腹部不适情况方面比较,差异无统计学意义(P>0.05)。结论将改良注水法应用于女性患者无痛结肠镜检查,能够缩短进镜时间,降低结肠镜检查难度,减少麻醉风险,且操作简便易行,值得临床应用。

微波强化改性膨润土的制备及其对磷吸附性能研究

利用FeSO4在微波辐照条件下制备改性膨润土,利用其对含磷废水进行处理,考察了微波辐照功率、辐照时间、FeSO4添加量等对制备条件的影响,实验结果表明,当微波辐照功率480W、微波辐照时间8min,FeSO4添加量为50mg时,改性膨润土的制备条件较好。除磷实验表明,当溶液pH值为6、接触时间15min、投加量5.0g时,改性膨润土对50mg/L含磷废水的去除率达到98%。

改性膨润土对酸性靛蓝的吸附性能研究

以广西宁明膨润土为原料,制备了酸化和铁柱撑两种改性膨润土,采用静态法研究了改性土对酸性靛蓝(AB74)染料的吸附性能。考察了吸附剂的投加量、吸附时间、温度和pH值等因素,对酸性靛蓝(AB74)染料吸附效果的影响,并研究了染料在改性土上的吸附平衡和吸附动力学过程。实验结果表明,酸化土和铁柱撑土对酸性靛蓝的最大吸附量分别为202.8mg/g和39.9mg/g。

层层自组装法制备双重修饰脂质体及其体外消化稳定性

以粒径、电位、包覆率、沉淀量为指标,采用层层自组装法制备海藻酸钠(sodium alginate,AL)、壳聚糖(chitosan,CH)双重修饰脂质体海藻酸钠-壳聚糖-脂质体(AL-CH-L),研究AL-CH-L的物化性质、微观形貌及其体外消化稳定性。结果表明:0.6%壳聚糖和0.5%海藻酸钠制备的AL-CH-L平均粒径为(330.6±37.3)nm,分布集中,Zeta电位为(-15.8±0.7)mV,透射电镜观察双重修饰脂质体的外层成功包裹了海藻酸钠和壳聚糖聚合物,呈现典型的核-壳结构。以钙黄绿素为荧光标记物对未修饰和双重修饰脂质体进行体外消化实验,结果表明双重修饰脂质体表现出更高的体外消化稳定性。

改良三点式重睑成形术的临床应用研究

目的:探讨改良三点式重睑术的临床应用疗效。方法:2012年6月-2019年6月,共165例单睑患者采用了改良三点式重睑术,沿术前标记线将三点处皮肤切开,剪刀将切口下唇的眼轮匝肌适当去除,同时将切口与切口在皮下层打通,剪除切口与切口之间的眼轮匝肌,6-0可吸收线挂睑板前筋膜或提上睑肌腱膜及切口下唇皮下组织缝合,三点切口各缝1针。再用6-0单丝尼龙线按照常规重睑线缝合方法挂切口下唇皮肤、睑板前筋膜或提上睑肌腱膜及切口上唇皮肤缝合打结,三点切口各缝1针。伴内眦赘皮者同时行内眦赘皮矫正术。结果:152例患者获得随访,随访患者大部分获得了比较满意的重睑,睁眼重睑流畅、自然,闭眼刀口痕迹不明显。2例患者出现内侧重睑线变浅,1例患者出现外侧重睑线变浅,所有患者均未出现重睑消失。5例患者双侧重睑线有轻度不对称。患者总体满意率为94.7%(144/152)。结论:改良三点式重睑术具有创伤小、并发症少、效果逼真、不易脱落、手术痕迹不明显等优点,值得推广应用。

改良Blalock-Taussig分流术在危重紫绀型先天性心脏病中的应用

目的 探讨改良Blalock Taussig分流术在危重紫绀型先天性心脏病中的应用,并对手术效果进行评价。方法 运用回顾性分析法,总结2 0 0 0年10月—2 0 0 4年4月本院2 5例患儿行改良Blalock Taussig分流术的临床经验。其中男15例,女10例,年龄3d～3岁,平均(3.0 7±3.4 3)月,新生儿8例,体重1.5～12kg ,平均(4.14±1.84 )kg。结果 术后死亡2例,死亡率8% (2 / 2 5 ) ,患儿动脉血氧饱和度由术前4 0 %～70 % (5 8.8%±10 .1% )上升至术后75 %～85 % (78.4 %±4 .3% ) ,有明显改善(P <0 .0 5 )。随访6～32月,平均14 .8月,紫绀明显减轻,活动耐量增加。6例患儿已行二期手术。结论 改良Blalock Taussig分流术作为姑息性手术可改善缺氧,促进肺血管发育,为二期施行双心室矫治及双向腔肺分流术或房坦手术(Fontan)赢得时机,减少死亡率。

改性膨润土预处理垃圾渗滤液的试验研究

垃圾渗滤液成分复杂,较难处理。膨润土具有较强的吸附性能和阳离子交换能力,价廉易得且可再生利用,所以可应用于污水处理中。通过对改性膨润土处理渗滤液的试验,发现:改性高效钠基土的处理效果最好,当渗滤液pH=6.5、投土量为4g/L时,对COD平均去除率为72%,对BOD平均去除率为70%,对NH3-N的平均去除率为41%,显著减轻了后续处理的负担。

改良经皮旋转扩张气管切开术与传统气管切开术效果比较

目的探讨改良经皮旋转扩张气管切开术在危重病中的应用价值。方法回顾性分析重症监护病房(ICU)中气管切开的危重病患者共126例,其中改良经皮扩张气管切开术组(PDT组)82例,常规外科气管切开术组(ST组)44例。比较两组手术操作时间、术中出血量、并发症发生率。结果改良PDT组的手术创伤和术后并发症较ST组明显减少。结论改良经皮旋转扩张气管切开术是一种快速、创伤小、并发症少的微创气管切开术。

应用依赖解旋酶DNA扩增技术检测转基因大豆

目的:基于依赖解旋酶DNA恒温扩增(helicase-dependent isothermal DNA amplifi cation,HDA)技术,建立快速检测转基因大豆的方法。方法:采用以CaMV35S、NOS、CP4-EPSPS 3种外源基因和内源基因Lectin(大豆凝集素基因)为目的片段设计4对特异引物,建立反应体系,通过HDA方法对内源基因和3种外源基因的检测特异性和灵敏度进行实验,并对结果进行同源性分析。结果:建立了转基因大豆HDA检测法,检测特异性良好,3种外源基因的检出限为0.2%。结论:转基因大豆的HDA检测方法具有普通聚合酶链式反应的特异、灵敏等特点,并且对仪器要求更低,极适合基层实验室使用,具有广阔的应用前景。