【目的】初步探讨青少期应激结合成年后诱导抑郁大鼠海马CA1区细胞形态与5-羟色胺转运体(5-HTT)蛋白表达的变化及加味四逆散的干预作用。【方法】将132只Wistar大鼠随机分成空白组、模型组、加味四逆散组、氟西汀组,共4组,每组33只;组...【目的】初步探讨青少期应激结合成年后诱导抑郁大鼠海马CA1区细胞形态与5-羟色胺转运体(5-HTT)蛋白表达的变化及加味四逆散的干预作用。【方法】将132只Wistar大鼠随机分成空白组、模型组、加味四逆散组、氟西汀组,共4组,每组33只;组内再随机分为青少期、成年期、成年后3个小组,每小组11只。选取44、56、78 d 3个时间点作为青少期、成年期、成年后3个阶段的取材时间点。复制慢性不可预知应激(CUMS)大鼠模型。于第21~44天、第57~78天造模及给药,第44~55天正常喂养,不予造模及给药。观察各组大鼠的一般状况与体质量变化,采用苏木素—伊红(HE)染色法观察海马CA1区细胞形态,采用免疫组化染色法观察海马CA1区5-HTT阳性细胞的分布变化。【结果】模型组青少期、成年期、成年后大鼠一般状况较差,而经加味四逆散及氟西汀治疗后的大鼠均得到明显改善。各阶段的模型组大鼠体质量较同阶段的空白组显著降低(P<0.01);成年后,与空白组比较,其余3组体质量均显著降低(P<0.01),且3组间比较,差异无统计学意义(P>0.05)。在海马CA1区细胞形态表现方面,模型组青少期较多神经元萎缩、深染,胞核、胞浆界限不清,成年期及成年后呈进行性加重,大量神经元深染,胞核、胞浆界限不清,并有少量胶质细胞增生;而氟西汀组、加味四逆散组与同阶段模型组比较,神经元萎缩、深染减少。5-HTT表达结果显示,成年期、成年后模型组5-HTT的平均光密度值较同阶段的空白组显著降低(P<0.05或P<0.01),成年后加味四逆散组,成年期、成年后氟西汀组5-HTT的平均光密度值值较同阶段的模型组有显著升高(P<0.05或P<0.01)。【结论】在青少期受到CUMS的大鼠可产生抑郁样行为,成年后继续施加刺激诱导会进一步加重抑郁状态。抑郁大鼠海马内5-HTT改变可能是加味四逆散干预作用的重要通路。展开更多
In recent years,the problem of privacy leakage has attracted increasing attentions.Therefore,machine learning privacy protection becomes crucial research topic.In this paper,the Paillier homomorphic encryption algorit...In recent years,the problem of privacy leakage has attracted increasing attentions.Therefore,machine learning privacy protection becomes crucial research topic.In this paper,the Paillier homomorphic encryption algorithm is proposed to protect the privacy data.The original LeNet-5 convolutional neural network model was first improved.Then the activation function was modified and the C5 layer was removed to reduce the number of model parameters and improve the operation efficiency.Finally,by mapping the operation of each layer in the convolutional neural network from the plaintext domain to the ciphertext domain,an improved LeNet-5 model that can run on encrypted data was constructed.The purpose of using machine learning algorithmwas realized and privacywas ensured at the same time.The analysis shows that the model is feasible and the efficiency is improved.展开更多
文摘【目的】初步探讨青少期应激结合成年后诱导抑郁大鼠海马CA1区细胞形态与5-羟色胺转运体(5-HTT)蛋白表达的变化及加味四逆散的干预作用。【方法】将132只Wistar大鼠随机分成空白组、模型组、加味四逆散组、氟西汀组,共4组,每组33只;组内再随机分为青少期、成年期、成年后3个小组,每小组11只。选取44、56、78 d 3个时间点作为青少期、成年期、成年后3个阶段的取材时间点。复制慢性不可预知应激(CUMS)大鼠模型。于第21~44天、第57~78天造模及给药,第44~55天正常喂养,不予造模及给药。观察各组大鼠的一般状况与体质量变化,采用苏木素—伊红(HE)染色法观察海马CA1区细胞形态,采用免疫组化染色法观察海马CA1区5-HTT阳性细胞的分布变化。【结果】模型组青少期、成年期、成年后大鼠一般状况较差,而经加味四逆散及氟西汀治疗后的大鼠均得到明显改善。各阶段的模型组大鼠体质量较同阶段的空白组显著降低(P<0.01);成年后,与空白组比较,其余3组体质量均显著降低(P<0.01),且3组间比较,差异无统计学意义(P>0.05)。在海马CA1区细胞形态表现方面,模型组青少期较多神经元萎缩、深染,胞核、胞浆界限不清,成年期及成年后呈进行性加重,大量神经元深染,胞核、胞浆界限不清,并有少量胶质细胞增生;而氟西汀组、加味四逆散组与同阶段模型组比较,神经元萎缩、深染减少。5-HTT表达结果显示,成年期、成年后模型组5-HTT的平均光密度值较同阶段的空白组显著降低(P<0.05或P<0.01),成年后加味四逆散组,成年期、成年后氟西汀组5-HTT的平均光密度值值较同阶段的模型组有显著升高(P<0.05或P<0.01)。【结论】在青少期受到CUMS的大鼠可产生抑郁样行为,成年后继续施加刺激诱导会进一步加重抑郁状态。抑郁大鼠海马内5-HTT改变可能是加味四逆散干预作用的重要通路。
基金The National Natural Science Foundation of China(No.61572521)Engineering University of PAP Innovation Team Science Foundation(No.KYTD201805)Natural Science Basic Research Plan in Shaanxi Province of China(2021JM252).
文摘In recent years,the problem of privacy leakage has attracted increasing attentions.Therefore,machine learning privacy protection becomes crucial research topic.In this paper,the Paillier homomorphic encryption algorithm is proposed to protect the privacy data.The original LeNet-5 convolutional neural network model was first improved.Then the activation function was modified and the C5 layer was removed to reduce the number of model parameters and improve the operation efficiency.Finally,by mapping the operation of each layer in the convolutional neural network from the plaintext domain to the ciphertext domain,an improved LeNet-5 model that can run on encrypted data was constructed.The purpose of using machine learning algorithmwas realized and privacywas ensured at the same time.The analysis shows that the model is feasible and the efficiency is improved.
