Experimental assessment of a modified retrograde traction tracheal intubation method for increasing the success rate of myocardial infarction model in rats

Background Animal models of myocardial infarction （MI） have been widely used to study the pathologi- cal and physiological changes that occur in MI, and to objectively evaluate the efficacy of new treatments. They are an important tool in this procedure. However, the mortality rate of MI animal models has so far been higher than in real-life situations. The aim of this study was to explore the use of a modified retrograde traction tracheal intubation （MRTI） method for increasing the success rate of MI models in rats. Methods Sixty male Sprague-Dawley rats were used in the experiment. Using the MRTI method of artificial airway generation, we established the MI model by ligation of the left anterior descending branch of the coronary artery. We analyzed the effects of MRTI, the use of lidocaine, operative details, nursing considerations during the operation, and post-operative factors on the success rate of the MI model in rats. Results The success rate of generating an MI model in rats can be significantly increased using the following methods： 1） Setting up the artificial airway through the use of MRTI by using a single-lumen central venous catheter; 2） Selecting a ligation site 2 mm be- low the midpoint of the connection between the left atrial appendage and the pulmonary cone; 3） Adding a drop of lidocaine to the surface of the heart to slow down the heart rate, make the operation easier to perform, and prevent arrhythmias postoperatively; 4） Clearing up airway secretions timely both intra and postoperative- ly; 5） Making sure that rats are in a warm state both intra and postoperatively; 6） Preventing wound infection. Conclusions Use of the MRTI method can quickly establish an artificial airway in rats. Intraoperative use of lidocaine, selecting a precise vascular ligation site, and appropriate care both intra and postoperatively can in- crease the success rate of MI model generation.

逆行牵引改良气管插管法提高大鼠心肌梗死模型成功率

目的研究采用逆行牵引改良气管插管法提高大鼠心肌梗死模型造模成功率的实验方法。方法 125只SD雄性大鼠,采用逆行牵引改良气管插管法(逆行插管组)和经口气管插管法(经口插管组)建立人工气道,结扎冠状动脉左前降支制作心肌梗死模型。4周后采用超声心动图检测大鼠心肌梗死模型心脏结构和心功能变化,采用Masson染色实验检测大鼠心脏胶原容积分数(CVF)。分析这两种气管插管方法、术中利多卡因的使用、手术操作过程中的细节问题、术中及术后护理事项等对大鼠模型成功率的影响。结果逆行牵引改良气管插管法建立大鼠人工气道的成功率为100%,明显高于传统的经口气管插管法(成功率为85%)。于左心耳与肺动脉圆锥连线下方2 mm进针结扎、结扎前滴1滴利多卡因到心脏表面减慢心率便于操作并预防心律失常、术中及术后及时清除气道分泌物、术中及术后保暖、预防伤口感染等方法,可提高大鼠心肌梗死模型的存活率。与正常大鼠比较,两组大鼠均出现左心室收缩末期内径变小,左心室后壁代偿性肥厚,左心室射血分数降低,心脏非心肌梗死区CVF增加;且两组间比较上述指标差异无统计学意义。逆行插管组心肌梗死模型造模成功率(76.7%)高于经口插管组(65.0%)。结论逆行牵引改良气管插管法的应用能够为大鼠心肌梗死模型的制作迅速备好人工气道,术中使用利多卡因、精准的血管结扎部位及合适的术中及术后护理对提高大鼠心肌梗死模型造模成功率具有重要意义。

改良逆行气管插管在呼吸衰竭患者中应用效果分析

目的:分析改良逆行气管插管在呼吸衰竭患者中应用效果。方法:研究对象来自我院2011年3月至2013年10月收治的100例呼吸衰竭伴有困难气道者,采用计算机随机分组的方式将患者分为对照组和观察组,每组各50例。对照组患者采用传统逆行气管插管法进行救治,观察组患者采用改良逆行气管插管法进行抢救,对比分析两组患者平均插管时间、并发症发生率及插管成功率(第一次、第二次、第三次)。结果:观察组患者平均插管时间和并发症发生率显著小于对照组患者,差异有统计学意义,P<0.05。观察组患者插管一次成功率显著高于对照组患者,差异有统计学意义,P<0.05。结论:改良逆行气管插管法能够明显缩短插管时间,减少插管并发症,明显提高插管一次成功率,值得临床推广应用。

改良逆行气管插管在呼吸衰竭中的综合应用价值研究

目的探讨改良逆行气管插管在呼吸衰竭中的综合应用价值。方法随机选取2011年3月-2012年8月于广东医学院附属石龙博爱医院采用改良逆行气管插管法进行抢救的30例呼吸衰竭伴困难气道患者为观察组,同期采用传统逆行气管插管法进行抢救的30例患者为对照组,后将两组患者的插管时间、第1,2,3次插管成功率、并发症发生率、插管前及插管中的血流动力学指标进行比较。结果观察组的插管时间短于对照组,第1次插管成功率高于对照组,并发症发生率低于对照组,插管中的SBP、DBP、HR及MAP均低于对照组,P均<0.05,差异均有统计学意义。结论改良逆行气管插管在呼吸衰竭中的综合应用价值较高,对患者的不良刺激更小。