A suction caisson can be extracted by applying reverse pumping water,which cannot be regarded as the reverse process of installation because of the dramatically different soil−structure interaction behavior.Model test...A suction caisson can be extracted by applying reverse pumping water,which cannot be regarded as the reverse process of installation because of the dramatically different soil−structure interaction behavior.Model tests were first carried out in this study to investigate the extraction behavior of the modified suction caisson(MSC)and the regular suction caisson(RSC)in sand by reverse pumping water.The effects of the installation ways(suction-assisted or jacking installation)and the reverse pumping rate on the variations of the over-pressure resulting form reverse pumping water were investigated.It was found that neither the RSC nor the MSC can be fully extracted from sand.When the maximum extraction displacement is obtained,the hydraulic gradient of the sand in the suction caisson reaches the critical value,leading to seepage failure.In addition,the maximum extraction displacement decreases with the increasing reverse pumping rate.Under the same reverse pumping rate,the final extraction displacements for the RSC and MSC installed by suction are lower than those for the RSC and MSC installed by jacking.The final extraction displacement of MSC is almost equal to that of the RSC with the same internal compartment length.Based on the force equilibrium,a method of estimating the maximum extraction displacement is proposed.It has been proved that the proposed method can rationally predict the maximum extraction displacement and the corresponding over-pressure.展开更多
Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by s...Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.展开更多
基金This study was financially supported by the National Natural Science Foundation of China(Grant Nos.51879044 and 51639002)the Shandong Natural Fund(Grant No.ZR2019BEE007)+1 种基金SDUST Research Fund(Grant No.2015TDJH104)the Project oce and Technology Program(Grant No.J18KA184).
文摘A suction caisson can be extracted by applying reverse pumping water,which cannot be regarded as the reverse process of installation because of the dramatically different soil−structure interaction behavior.Model tests were first carried out in this study to investigate the extraction behavior of the modified suction caisson(MSC)and the regular suction caisson(RSC)in sand by reverse pumping water.The effects of the installation ways(suction-assisted or jacking installation)and the reverse pumping rate on the variations of the over-pressure resulting form reverse pumping water were investigated.It was found that neither the RSC nor the MSC can be fully extracted from sand.When the maximum extraction displacement is obtained,the hydraulic gradient of the sand in the suction caisson reaches the critical value,leading to seepage failure.In addition,the maximum extraction displacement decreases with the increasing reverse pumping rate.Under the same reverse pumping rate,the final extraction displacements for the RSC and MSC installed by suction are lower than those for the RSC and MSC installed by jacking.The final extraction displacement of MSC is almost equal to that of the RSC with the same internal compartment length.Based on the force equilibrium,a method of estimating the maximum extraction displacement is proposed.It has been proved that the proposed method can rationally predict the maximum extraction displacement and the corresponding over-pressure.
基金supported by the National Transgenic Major Program of China(No.2019ZX08010-002)the China Agriculture Research System(No.CARS-21)the Major Science and Technology Projects of Breeding New Varieties of Agriculture in Zhejiang Province,China(No.2016C02058)。
文摘Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.
