In order to diagnose the diseased pigs in a certain large pig farm in Binzhou City,Shandong Province,the dead piglets with joint swelling were subjected to necroscopy,and the pathogenic bacterium was isolated and iden...In order to diagnose the diseased pigs in a certain large pig farm in Binzhou City,Shandong Province,the dead piglets with joint swelling were subjected to necroscopy,and the pathogenic bacterium was isolated and identified. One Gram-positive Streptococcus was isolated. The strain was subjected to characteristic culture,microscopic examination and molecular biological identification,and resistance detection,animal regression experiment and mouse pathogenicity test were carried out. The results showed that the isolate was identified to be Streptococcus suis serotype 7,which was resistant to multiple drugs; and the pathogenicity test showed that the strain had high pathogenicity to pigs,resulting in neurosis on partial pigs,and the strain had no pathogenicity to Kunming and BALB/c mice but certain pathogenicity to CD1 mice.展开更多
An endophytic fungus MOD-1 was isolated from the roots of mulberry trees,and was identified as Macrophomina phaseolina by morphology and molecular biology.The 5.8 S rDNA/ITS region sequence of the strain has been regi...An endophytic fungus MOD-1 was isolated from the roots of mulberry trees,and was identified as Macrophomina phaseolina by morphology and molecular biology.The 5.8 S rDNA/ITS region sequence of the strain has been registered in GeneBank with the accession number of EU250575.展开更多
基金Supported by Natural Science Foundation of Shandong Province(ZR2014CQ009)Binzhou Municipal Science and Technology Project(2013GG0304)
文摘In order to diagnose the diseased pigs in a certain large pig farm in Binzhou City,Shandong Province,the dead piglets with joint swelling were subjected to necroscopy,and the pathogenic bacterium was isolated and identified. One Gram-positive Streptococcus was isolated. The strain was subjected to characteristic culture,microscopic examination and molecular biological identification,and resistance detection,animal regression experiment and mouse pathogenicity test were carried out. The results showed that the isolate was identified to be Streptococcus suis serotype 7,which was resistant to multiple drugs; and the pathogenicity test showed that the strain had high pathogenicity to pigs,resulting in neurosis on partial pigs,and the strain had no pathogenicity to Kunming and BALB/c mice but certain pathogenicity to CD1 mice.
基金Supported by National Cocoon and Silk Development Risk Fund(GJXBH 200732)Natural Science Foundation of Shandong Province(2007ZRB01872).
文摘An endophytic fungus MOD-1 was isolated from the roots of mulberry trees,and was identified as Macrophomina phaseolina by morphology and molecular biology.The 5.8 S rDNA/ITS region sequence of the strain has been registered in GeneBank with the accession number of EU250575.
