Molecular imprinting technology is applied in surface plasmon resonance spectroscopy for highly sensitive and selective detection of morphine（MO）.As SPR-based sensor of MO,the preparation of molecular imprinted poly...Molecular imprinting technology is applied in surface plasmon resonance spectroscopy for highly sensitive and selective detection of morphine（MO）.As SPR-based sensor of MO,the preparation of molecular imprinted polymer is as follows： methacryhc acids（MAA）,ethylene glycol dimethacrylate（EGDMA）,azodiisobutyronitrile（AIBN） were used as functional monomer,cross-linker and initiator,respectively.The experiment results showed that morphine imprinted polymer had the performance of high sensitivity and specificity,i.e.the relative signal of SPR response was proportional to the concentration of morphine in acetonitrile in the range of 10^（-9）mol/L to 10^（-6）mol/L（1 ppb-1 ppm） with LOD of 10^（-10）mol/L,and MO was distinguished from its analogs,such as codeine.展开更多
In this study,the patulin imprinted and the non-imprinted nanoparticles are synthesized by the two-phase mini emulsion polymerization method and characterized by zeta-size analysis,Fourier transform infrared spectrosc...In this study,the patulin imprinted and the non-imprinted nanoparticles are synthesized by the two-phase mini emulsion polymerization method and characterized by zeta-size analysis,Fourier transform infrared spectroscopy,and scanning electron microscopy.Afterwards,the patulin imprinted and the non-imprinted nanoparticles are attached on the surface of surface plasmon resonance(SPR)chips.The patulin imprinted and the non-imprinted SPR nanosensors are characterized by using atomic force microscope,ellipsometer,and contact angle measurements.Kinetic studies for patulin detection are carried out in the concentration range of 0.5nmol-750nmol.The limit of detection and the limit of quantification values are obtained as 0.011 nmol and 0.036 nmol,respectively.In all kinetic analysis,the response time is 13 min for equilibration,adsorption,and desorption cycles.The selectivity studies of the patulin imprinted and the non-imprinted SPR nanosensors are determined in the presence of ochratoxin A and aflatoxin Bl.In order to demonstrate the applicability,validation studies of the patulin imprinted SPR nanosensor are performed by liquid chromatography-tandem mass spectrometry(LC-MS).展开更多
基金the Innovation Project of the Ministry of Public Security of the People's Republic of China(No. 2008YYCXGKXXS130)Academician Foundation of the Ministry of Public Security of the People's Republic of China(No.2011-23210044)the National Natural Science Foundation of China(No.81001348) for their financial supports
文摘Molecular imprinting technology is applied in surface plasmon resonance spectroscopy for highly sensitive and selective detection of morphine（MO）.As SPR-based sensor of MO,the preparation of molecular imprinted polymer is as follows： methacryhc acids（MAA）,ethylene glycol dimethacrylate（EGDMA）,azodiisobutyronitrile（AIBN） were used as functional monomer,cross-linker and initiator,respectively.The experiment results showed that morphine imprinted polymer had the performance of high sensitivity and specificity,i.e.the relative signal of SPR response was proportional to the concentration of morphine in acetonitrile in the range of 10^（-9）mol/L to 10^（-6）mol/L（1 ppb-1 ppm） with LOD of 10^（-10）mol/L,and MO was distinguished from its analogs,such as codeine.
基金This study was partly supported by Scientific Research Foundation of Hacettepe University Project(Grant No.FHD-2019-18247).
文摘In this study,the patulin imprinted and the non-imprinted nanoparticles are synthesized by the two-phase mini emulsion polymerization method and characterized by zeta-size analysis,Fourier transform infrared spectroscopy,and scanning electron microscopy.Afterwards,the patulin imprinted and the non-imprinted nanoparticles are attached on the surface of surface plasmon resonance(SPR)chips.The patulin imprinted and the non-imprinted SPR nanosensors are characterized by using atomic force microscope,ellipsometer,and contact angle measurements.Kinetic studies for patulin detection are carried out in the concentration range of 0.5nmol-750nmol.The limit of detection and the limit of quantification values are obtained as 0.011 nmol and 0.036 nmol,respectively.In all kinetic analysis,the response time is 13 min for equilibration,adsorption,and desorption cycles.The selectivity studies of the patulin imprinted and the non-imprinted SPR nanosensors are determined in the presence of ochratoxin A and aflatoxin Bl.In order to demonstrate the applicability,validation studies of the patulin imprinted SPR nanosensor are performed by liquid chromatography-tandem mass spectrometry(LC-MS).
