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Optimal multilevel thresholding based on molecular kinetic theory optimization algorithm and line intercept histogram 被引量:3
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作者 范朝冬 任柯 +1 位作者 张英杰 易灵芝 《Journal of Central South University》 SCIE EI CAS CSCD 2016年第4期880-890,共11页
Among all segmentation techniques, Otsu thresholding method is widely used. Line intercept histogram based Otsu thresholding method(LIH Otsu method) can be more resistant to Gaussian noise, highly efficient in computi... Among all segmentation techniques, Otsu thresholding method is widely used. Line intercept histogram based Otsu thresholding method(LIH Otsu method) can be more resistant to Gaussian noise, highly efficient in computing time, and can be easily extended to multilevel thresholding. But when images contain salt-and-pepper noise, LIH Otsu method performs poorly. An improved LIH Otsu method(ILIH Otsu method) is presented, which can be more resistant to Gaussian noise and salt-and-pepper noise. Moreover, it can be easily extended to multilevel thresholding. In order to improve the efficiency, the optimization algorithm based on the kinetic-molecular theory(KMTOA) is used to determine the optimal thresholds. The experimental results show that ILIH Otsu method has stronger anti-noise ability than two-dimensional Otsu thresholding method(2-D Otsu method), LIH Otsu method, K-means clustering algorithm and fuzzy clustering algorithm. 展开更多
关键词 分子动理论 优化算法 直方图 动力学理论 阈值分割 OTSU法 OTSU方法 最大类间方差法
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Breeding Rice Restorer Lines with High Resistance to Bacterial Blight by Using Molecular Marker-Assisted Selection 被引量:1
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作者 DENG Qi-ming WANG Shi-quan +2 位作者 ZHENG Ai-ping ZHANG Hong-yu LI Ping 《Rice science》 SCIE 2006年第1期22-28,共7页
Two bacterial blight (BB) resistance genes, Xa21 and Xa4, from IRBB24 were introduced into hybrid rice restorer line Mianhui 725, which is highly susceptible to BB, by using hybridization and molecular marker-assist... Two bacterial blight (BB) resistance genes, Xa21 and Xa4, from IRBB24 were introduced into hybrid rice restorer line Mianhui 725, which is highly susceptible to BB, by using hybridization and molecular marker-assisted selection technology. Four homologous restorer lines were obtained through testing the R target genes with molecular markers and analyzing parental genetic background. Inoculation of the four lines and their hybrids with the specific strains of Xanthomonas oryzae pv. oryzae, P1, P6 and seven representative strains of Chinese pathotype, C Ⅰ -CⅦ, showed that all of the four lines and their hybrids were highly resistant and presented broad resistance-spectrum to BB. The hybrids of G46A / R207-2 displayed good agronomic characters and high yield potential, and R207-2 was named Shuhui 207. 展开更多
关键词 bacterial blight RESISTANCE gene pyramiding hybrid rice restorer line molecular marker-assisted selection BREEDING
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Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection 被引量:1
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作者 LIU Wu-ge JIN Su-juan +7 位作者 ZHU Xiao-yuan WANG Feng LI Jin-hua LIU Zhen-rong LIAO Yi-long ZHU Man-shan HUANG Hui-jun LIU Yi-bai 《Rice science》 SCIE 2008年第3期179-185,共7页
The broad-spectrum blast resistance gene Pi-1, from donor line BL122, was introduced into a thermo-sensitive genic male sterile rice line GD-8S, which possessed good grain quality but high susceptibility to rice blast... The broad-spectrum blast resistance gene Pi-1, from donor line BL122, was introduced into a thermo-sensitive genic male sterile rice line GD-8S, which possessed good grain quality but high susceptibility to rice blast, by using backcross breeding and molecular marker-assisted selection. Five elite improved male sterile lines, RGD8S-1, RGD8S-2, RGD8S-3, RGD8S-4 and RGD8S-5, were selected based on the results of molecular marker analysis, spikelet sterility, recovery rate of genetic background and agronomic traits. Thirty-three representative blast isolates collected from Guangdong Province, China were used to inoculate the improved lines and the original line GD-8S artificially. The resistance frequencies of the improved lines ranged from 76.47% to 100%, much higher than that of the original line GD-8S (9.09%). On the agronomic characters, there were no significant differences between the improved lines and GD-8S except for flag leaf length and panicle number per plant. The improved lines could be used for breeding hybrid rice with high blast resistance. 展开更多
关键词 RICE blast resistance thermo-sensitive genic male sterile line molecular marker-assisted selection BREEDING
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Genetic Analysis and Molecular Mapping of an All-Stage Stripe Rust Resistance Gene in Triticum aestivum-Haynaldia villosa Translocation Line V3 被引量:3
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作者 HOU Lu MA Dong-fang +3 位作者 HU Mao-lin HE Miao-miao LU Yan JING Jin-xue 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第12期2197-2208,共12页
Triticum aestivum-Hayaldia villosa translocation line V3 has shown effective all-stage resistance to the seven dominant pathotypes of Puccinia striiforms f.sp.tritici prevalent in China.To elucidate the genetic basis ... Triticum aestivum-Hayaldia villosa translocation line V3 has shown effective all-stage resistance to the seven dominant pathotypes of Puccinia striiforms f.sp.tritici prevalent in China.To elucidate the genetic basis of the resistance,the segregating populations were developed from the cross between V3 and susceptible genotype Mingxian 169,seedlings of the parents and F 2 progeny were tested with six prevalent pathotypes,including CYR29,CYR31,CYR32-6,CYR33,Sun11-4,and Sun11-11,F 1 plants and F 3 lines were also inoculated with Sun11-11 to confirm the result further.The genetic studied results showed that the resistance of V3 against CYR29 was conferred by two dominant genes,independently,one dominant gene and one recessive gene conferring independently or a single dominant gene to confer resistance to CYR31,two complementary dominant genes conferring resistance to both CYR32-6 and Sun11-4,two independently dominant genes or three dominant genes(two of the genes show cumulative effect) conferring resistance to CYR33,a single dominant gene for resistance to Sun11-11.Resistance gene analog polymorphism(RGAP) and simple-sequence repeat(SSR) techniques were used to identify molecular markers linked to the single dominant gene(temporarily designated as YrV3) for resistance to Sun11-11.A linkage map of 2 RGAP and 7 SSR markers was constructed for the dominant gene using data from 221 F 2 plants and their derived F 2:3 lines tested with Sun11-11 in the greenhouse.Amplification of the complete set of nulli-tetrasomic lines of Chinese Spring with a RGAP marker RG1 mapped the gene on the chromosome 1B,and then the linked 7 SSR markers located this gene on the long arm of chromosome 1B.The linkage map spanned a genetic distance of 25.0 cM,the SSR markers Xgwm124 and Xcfa2147 closely linked to YrV3 with genetic distances of 3.0 and 3.8 cM,respectively.Based on the linkage map,it concluded that the resistance gene YrV3 was located on chromosome arm 1BL.Given chromosomal location,the reaction patterns and pedigree analysis,YrV3 should be a novel gene for resistance to stripe rust in wheat.These closely linked markers should be useful in stacking genes from different sources for wheat breeding and diversification of resistance genes against stripe rust. 展开更多
关键词 Haynaldia villosa translocation line stripe rust RGAP-SSR molecular mapping
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Genetic Diversity Analysis of 13 Maize Inbred Lines by SSR Molecular Markers
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作者 Yi ZHONG Yueying LI Xiuwen ZHANG 《Asian Agricultural Research》 2017年第7期80-82,共3页
This paper used SSR molecular markers to perform the genetic diversity analysis on 13 ordinary inbred lines of maize of different sources in order to divide heterotic groups of maize inbred line and predict heterosis.... This paper used SSR molecular markers to perform the genetic diversity analysis on 13 ordinary inbred lines of maize of different sources in order to divide heterotic groups of maize inbred line and predict heterosis. Using 12 pairs of SSR primers,a total of 47 allelic variants were detected in 13 inbred lines,2-5 alleles were detected for each pair of primers,an average of 3. 9,and polymorphism information content varied from 0. 379 to 0. 828. According to the cluster analysis,the 13 inbred lines could be divided into 5 groups. 展开更多
关键词 SSR molecular markers MAIZE Inbred line Genetic diversity
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Methods for the Determination of Stable Isotopes of Carbon and Nitrogen Directly in Valine, Proline, Glutamine, and Glutamic Acid
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作者 Lamzira Farulava Levani Eliashvili +1 位作者 Vakhtang Betlemidze Bachana Sulava 《American Journal of Analytical Chemistry》 2023年第10期467-480,共14页
Amino acids are very important compounds for the body and are involved in important functions that keep us healthy. Amino acids are essential components such as valine, proline, glutamine and glutamic acid. They can b... Amino acids are very important compounds for the body and are involved in important functions that keep us healthy. Amino acids are essential components such as valine, proline, glutamine and glutamic acid. They can be synthesized either naturally or artificially. To examine the metabolism and regulate the synthesis process, compounds labeled with nitrogen or carbon isotopes need to be used. These isotopic compounds allow for more extensive research and enable studies that would otherwise be impossible. However, their use is dependent on the availability of simple, efficient methods for isotopic analysis. Currently, the determination of the atomic fraction of carbon and nitrogen isotopes is only possible through their conversion into molecular nitrogen or carbon monoxide or carbon dioxide. This leads to the loss of information about isotopic enrichment in specific centers of the molecule. This article explores a new direct approach to determining the atomic fraction of carbon and nitrogen isotopes in the isotope-modified or identical centers of these compounds. This method eliminates the transfer process and dilution due to nitrogen and carbon impurities. It is now possible to simultaneously determine the atomic fraction of nitrogen and carbon isotopes in the research substance. This method can be applied to amino acids, making it an effective tool for proposing new research methods. Several articles [1] [2] [3] have proposed similar methods for organic compounds and amino acids. 展开更多
关键词 VAline PROline GLUTAMINE Glutamic Acid Mass Spectrometer Mass Spectrum Ion Current INTENSITY Mass lines molecular and Fragment Ions
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Preliminary Phenotypic and SNP-Based Molecular Characterization of Maize (<i>Zea mays</i>L.)-Mexicana (<i>Zea mays</i>SSP. <i>Mexicana</i>) Introgression Lines under Inbred Background of 48-2
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作者 Abdoul Raouf Sayadi Maazou Ju Qiu +13 位作者 Ling Ai Mohammad G. Arabzai Mengya Qian Hameed Gul Xuemei Du Fangyu Xing Jiarui Yan Keyi Zhang Ziqi Zhang Qiannan Ma Tianhui Huang Hong Duan Qianlin Xiao Zhizhai Liu 《American Journal of Plant Sciences》 2021年第7期1073-1089,共17页
Wild relatives possess potential genetic diversity for maize (<i><span style="font-family:Verdana;">Zea mays</span></i><span style="font-family:Verdana;"> L.) improvem... Wild relatives possess potential genetic diversity for maize (<i><span style="font-family:Verdana;">Zea mays</span></i><span style="font-family:Verdana;"> L.) improvement. Characterization of maize-</span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;"> introgression lines (ILs) is of great value to diversify the genetic base and improve the maize germplasm. Four maize-</span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;"> IL generations, </span><i><span style="font-family:Verdana;">i.e.</span></i><span style="font-family:Verdana;"> BC1, BC2, BC3, and RIL, were constructed under the elite inbred background of 48-2, elite inbred line that is widely used in maize breeding in Southwestern China, and were phenotyped in different years and genotyped with 56110 SNPs. The results indicated that 48-2 had higher phenotypic performances than all the characterized ILs on most of the agronomic traits. Compared with other ILs, BC2 individuals exhibited more similar performance to 48-2 on most traits and possessed the highest kernel ratio (66.5%). Population structure and principal component analysis indicated that BC3 individuals gathered closer to 48-2 and exhibited the lowest </span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;">-introgression frequency (0.50%), while BC2 (29.06%) and RIL (18.52%) showed higher introgression frequency. The high level of genetic diversity observed in the maize-</span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;"> ILs demonstrated that </span><i><span style="font-family:Verdana;">Z</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">mays</span></i><span style="font-family:Verdana;"> ssp. </span><i><span style="font-family:Verdana;">mexicana </span></i><span style="font-family:Verdana;">can serve as a potential source for the enrichment of maize germplasm.</span> 展开更多
关键词 Maize (Zea mays L.) Mexicana (Zea mays SSP. Mexicana) Introgression lines Phenotypic Characterization molecular Characterization
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抗稻瘟病水稻恢复系的分子标记辅助选育及抗性鉴定
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作者 孙富 唐梅 +2 位作者 卢宏琮 何聪 廖张波 《南方农业学报》 CAS CSCD 北大核心 2024年第4期1070-1078,共9页
【目的】开展抗稻瘟病水稻恢复系的分子标记辅助选育及抗性鉴定,为育成持久、广谱抗病优质杂交水稻新品种提供优异的遗传资源和技术参考。【方法】利用分子标记辅助选择和回交育种技术,将持久、广谱的抗稻瘟病基因Pi1、Pi2和Pigm导入恢... 【目的】开展抗稻瘟病水稻恢复系的分子标记辅助选育及抗性鉴定,为育成持久、广谱抗病优质杂交水稻新品种提供优异的遗传资源和技术参考。【方法】利用分子标记辅助选择和回交育种技术,将持久、广谱的抗稻瘟病基因Pi1、Pi2和Pigm导入恢复系桂R1703,获得了单基因、双基因和三基因导入系,并对其进行稻瘟病鉴定和农艺性状调查,分析不同抗稻瘟病基因间的抗性效应及抗性基因导入对受体亲本农艺性状的影响。【结果】通过对杂交后代和回交群体的Pi1、Pi2和Pigm基因相关分子标记检测,发现抗稻瘟病基因Pi1、Pi2和Pigm已成功导入到桂R1703中,筛选获得7个携带有抗稻瘟病基因且抗性和农艺性状综合表现优良的导入株系,表现出较强的稻瘟病抗性。单基因导入系中,抗稻瘟病基因的抗性效应排序为为Pigm>Pi2>Pi1;双基因和三基因导入系的稻瘟病抗性均强于单基因导入系,尤其以三基因导入系抗性最强,不同基因聚合的抗性效应排序为Pi1+Pi2+Pigm>Pi2+Pigm>Pi1+Pigm>Pi1+Pi2。7个瘟病抗性基因导入株系与桂R1703在穗长和千粒重方面无显著差异(P>0.05,下同),有2个瘟病抗性基因导入系的株高显著高于桂R1703(P<0.05,下同);有1个瘟病抗性基因导入系的单株有效穗显著高于桂R1703;有3个瘟病抗性基因导入系的单穗总粒数显著高于桂R1703;有5个瘟病抗性基因导入系结实率显著低于桂R1703;有2个瘟病抗性基因导入系的单株产量显著低于桂R1703;有4个瘟病抗性基因导入系的单株产量显著高于桂R1703。【结论】通过分子标记辅助选育可有效聚合多基因(Pi1、Pi2和Pigm基因),使恢复系桂R1703的稻瘟病抗性得到明显提升,获得一系列抗稻瘟病且与亲本其他性状相近的遗传材料,可作为亲本材料用于选育抗病优质的杂交稻。 展开更多
关键词 水稻 恢复系 稻瘟病 抗性基因 分子标记辅助育种 抗性评价
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BRAF V600E型转移性结直肠癌治疗进展
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作者 孟令桦 潘利华 +2 位作者 李瑞东 孙力军(综述) 叶书成(审校) 《中国肿瘤临床》 CAS CSCD 北大核心 2024年第4期209-213,共5页
转移性结直肠癌(metastatic colorectal cancer,mCRC)患者中约8%~15%存在BRAF基因突变,V600E是BRAF最常见的突变形式。BRAF V600E突变型的mCRC患者预后差,初始标准化疗效果不佳,强烈治疗的效果有限,并且在初始治疗失败后,后续治疗疗效甚... 转移性结直肠癌(metastatic colorectal cancer,mCRC)患者中约8%~15%存在BRAF基因突变,V600E是BRAF最常见的突变形式。BRAF V600E突变型的mCRC患者预后差,初始标准化疗效果不佳,强烈治疗的效果有限,并且在初始治疗失败后,后续治疗疗效甚微,疾病进展迅速,患者总生存期短,这类患者治疗现状不容乐观。因此,应寻找对于BRAF V600E突变型mCRC患者的更为有效治疗方案。本文将详细介绍BRAF V600E突变与mCRC患者预后及疗效预测方面的相关研究及治疗进展,以期为延长患者生存期提供参考依据。 展开更多
关键词 转移性结直肠癌 BRAF V600E 一线治疗 分子靶向治疗 免疫治疗
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基于自编码器的汽油分子组成预测
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作者 蔡广庆 胡益炯 +2 位作者 李春澎 纪晔 王弘历 《辽宁石油化工大学学报》 CAS 2024年第2期1-6,共6页
汽油分子在线调和技术需要快速获取各种类型组分油的详细分子组成信息。开发了基于自编码器的汽油分子组成快速解析方法,该方法可由近红外光谱直接预测汽油详细单体烃的组成。构建的汽油分子组成自编码器模型可挖掘汽油组成的潜在特征,... 汽油分子在线调和技术需要快速获取各种类型组分油的详细分子组成信息。开发了基于自编码器的汽油分子组成快速解析方法,该方法可由近红外光谱直接预测汽油详细单体烃的组成。构建的汽油分子组成自编码器模型可挖掘汽油组成的潜在特征,并利用潜在特征解码恢复原始分子组成。利用神经网络算法关联近红外光谱特征信息与汽油组成的潜在特征,并采用加氢汽油验证了模型的准确性。结果表明,平均绝对误差为0.033。开发的模型将自编码器算法应用在传统的石油化工过程中,对汽油分子在线调和与实时优化具有重要的指导意义。 展开更多
关键词 在线调和 自编码器 分子组成 快速解析 近红外光谱
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水稻香型抗病恢复系的分子鉴定及其应用
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作者 张玉婷 王泓超 +5 位作者 梁莹莹 匡梦鑫 吴欣 蒋云林 程祖锌 林荔辉 《中国农学通报》 2024年第18期90-95,共6页
提高水稻恢复系的稻米品质及稻瘟病抗性,是培育高产、优质、抗病的杂交稻新组合基础。采用香味fgr基因和稻瘟病抗性基因Pi-1、Pi-9和Pi-kh紧密连锁的分子标记,对香型品种‘玉针香’与抗病恢复系‘福恢683’‘、金恢1131’杂交获得的265... 提高水稻恢复系的稻米品质及稻瘟病抗性,是培育高产、优质、抗病的杂交稻新组合基础。采用香味fgr基因和稻瘟病抗性基因Pi-1、Pi-9和Pi-kh紧密连锁的分子标记,对香型品种‘玉针香’与抗病恢复系‘福恢683’‘、金恢1131’杂交获得的265个F_(6)代恢复株系进行香味和稻瘟病抗性基因的分子标记辅助选择,筛选出携带上述4个目的基因的6个优良恢复株系,通过综合性状考察,育成了中抗稻瘟病的香型恢复系‘润香’,并配组出高产、优质的香型黑米杂交稻新组合‘紫两优润香’先后通过广西(桂审稻2021200号)和福建省(闽审稻20220024)农作物新品种审定。该品种表现出高产、优质和广适应特性,具有较大的推广应用潜力。本研究表明,分子标记辅助选择是一种在水稻香味和稻瘟病抗性定向改良中的快速而有效的现代生物育种技术。 展开更多
关键词 水稻 恢复系 稻瘟病 香味 分子鉴定
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前进接触线薄液膜结构与运移机制
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作者 刘乔 黄家宸 +1 位作者 王昊 邓亚骏 《物理学报》 SCIE EI CAS CSCD 北大核心 2024年第1期277-284,共8页
气-液-固三相接触线薄液膜微观结构认识不清使得动态接触角预测问题始终存在争议.本文通过大规模分子动力学模拟研究液滴动态润湿过程,获得了完全浸润液滴前进接触线区域的前驱液膜结构、部分浸润液滴前进接触线区域的纳米级凸起结构.... 气-液-固三相接触线薄液膜微观结构认识不清使得动态接触角预测问题始终存在争议.本文通过大规模分子动力学模拟研究液滴动态润湿过程,获得了完全浸润液滴前进接触线区域的前驱液膜结构、部分浸润液滴前进接触线区域的纳米级凸起结构.首次在模拟中获得了微观接触角与宏观接触角随时间的动态演变规律.分子轨迹追踪揭示了接触线薄液膜区域流体在固体表面摩擦作用下,随速度增大从滑动为主向滚动为主的模式转变,进而在底层出现气体裹挟导致滑移、发生溅射的机制.本研究为进一步构建普适的动态接触角预测模型提供了关键物理依据. 展开更多
关键词 接触线 薄液膜 润湿 界面 分子模拟
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猪睾丸Dickkopf样顶体蛋白1基因的转录调控分析
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作者 许静 代红梅 +6 位作者 张霞 刘志朋 杨忠 李卫真 付仕颖 克比努尔·库尔班 霍金龙 《福建农林大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第1期79-88,共10页
【目的】Dickkopf样顶体蛋白1(DKKL1)是一种重要的顶体蛋白,为发掘其重要功能及潜在的临床价值,以版纳微型猪近交系(BMI)为对象,研究其睾丸组织中DKKL1的分子结构、转录调控特征和蛋白质功能。【方法】通过全转录组测序获得BMI DKKL1的... 【目的】Dickkopf样顶体蛋白1(DKKL1)是一种重要的顶体蛋白,为发掘其重要功能及潜在的临床价值,以版纳微型猪近交系(BMI)为对象,研究其睾丸组织中DKKL1的分子结构、转录调控特征和蛋白质功能。【方法】通过全转录组测序获得BMI DKKL1的表达量,使用逆转录聚合酶链反应(RT-PCR)获得DKKL1编码区序列并分析其基因结构和蛋白质特征,使用UniProt数据库对DKKL1进行功能注释并分析DKKL1与微小RNA(miRNA)、长链非编码RNA(lncRNA)间的竞争性内源RNA(ceRNA)转录调控。【结果】获得的BMI DKKL1编码区全长为702 bp,位于基因第6号染色体上,有5个外显子和4个内含子;蛋白质功能分析表明,DKKL1包含233个氨基酸,具有疏水性,含磷酸化位点、信号肽和较多的无规则卷曲亚结构;系统进化和同源性分析表明,DKKL1氨基酸序列在哺乳动物间高度保守;蛋白互作分析显示,DKKL1与含螺旋结构域的蛋白155(CCDC155)、转录增强缔合域蛋白2(TEAD2)、RAS癌基因家族成员11B(RAB11B)等多种蛋白互作;基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析表明,这些蛋白富集在典型Wnt信号等通路上;GO功能注释表明,DKKL1在睾酮生物合成过程的负向调控、透明带穿透、顶体泡生成等方面具有重要功能;ceRNA转录调控网络分析表明,DKKL1被miR-15a和miR-15b靶向调控。【结论】本研究获得了BMI睾丸全转录数据,阐明了DKKL1的分子特征、蛋白质功能并构建了转录调控网络。 展开更多
关键词 版纳微型猪近交系 Dickkopf样顶体蛋白1 分子特征 蛋白互作 调控网络
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Development and genetic analysis of wheat double substitution lines carrying Hordeum vulgare 2H and Thinopyrum intermedium 2Ai#2 chromosomes 被引量:1
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作者 Jing Wang Chang Liu +4 位作者 Xianrui Guo Ke Wang Lipu Du Zhishan Lin Xingguo Ye 《The Crop Journal》 SCIE CAS CSCD 2019年第2期163-175,共13页
Thinopyrum intermedium and barley are two close relatives of wheat and carry many genes that are potentially valuable for the improvement of various wheat traits. In this study we created wheat double substitution lin... Thinopyrum intermedium and barley are two close relatives of wheat and carry many genes that are potentially valuable for the improvement of various wheat traits. In this study we created wheat double substitution lines by hybridizing different wheat–Th. intermedium and wheat–barley disomic alien substitution lines, with the aim of using genes in Th. intermedium and barley for wheat breeding and investigating the genetic behavior of alien chromosomes and their wheat homoeologs. As expected, we obtained two types of wheat double substitution lines,2D2Ai#2(2B)2H( A) and 2A2 Ai#2(2B)2H(2D), in which different group 2 wheat chromosomes were replaced by barley chromosome 2 H and Th. intermedium chromosome 2Ai#2. The new materials were characterized using molecular markers, genomic in situ hybridization(GISH), and fluorescent in situ hybridization(FISH). GISH and FISH experiments revealed that the double substitution lines harbor 42 chromosomes including 38 wheat chromosomes, a pair of barley chromosomes, and a pair of Th. intermedium chromosomes. Analysis using specific DNA markers showed that two pairs of wheat homoeologous group 2 chromosomes in the new lines were substituted by a pair of 2H and a pair of 2Ai#2 chromosomes. Chromosome 2H showed a higher transmission rate than 2Ai#2, and both chromosomes were preferentially transmitted between generations via female gametes. Evaluation of botanic and agronomic traits demonstrated that,compared with their parents, the new lines showed similar growth habits and plant type but differences in plant height, flowering date, and self-fertility. Cytological observations using different probes suggested that the double substitution lines showed nearly normal genetic behavior before and during meiosis. The novel substitution lines can potentially be used in wheat meiosis research and breeding programs. 展开更多
关键词 WHEAT DOUBLE substitution lines molecular markers Transmission rate of ALIEN chromosomes Genomic in SITU hybridization
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低分子肝素联合多西他赛二线治疗驱动基因阴性晚期非小细胞肺癌的临床观察
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作者 毛延涛 姜茂竹 《中国当代医药》 CAS 2024年第12期91-94,99,共5页
目的探讨低分子肝素(LMWH)联合多西他赛二线治疗驱动基因阴性晚期非小细胞肺癌(NSCLC)的临床观察。方法选取2017年10月至2020年9月烟台市烟台山医院收治的符合标准的100例晚期NSCLC患者作为研究对象,按照随机数字表法分为研究组和对照组... 目的探讨低分子肝素(LMWH)联合多西他赛二线治疗驱动基因阴性晚期非小细胞肺癌(NSCLC)的临床观察。方法选取2017年10月至2020年9月烟台市烟台山医院收治的符合标准的100例晚期NSCLC患者作为研究对象,按照随机数字表法分为研究组和对照组,每组50例。研究组采用多西他赛化疗联合LMWH的方法。对照组采用多西他赛单药化疗的方法,直到疾病进展或不良反应不可耐受。比较两组治疗效果、凝血功能变化及不良反应。结果治疗2周期后疗效评价,两组患者的客观缓解率比较,差异无统计学意义(P>0.05)。治疗后,研究组疾病控制率高于对照组,差异有统计学意义(P<0.05)。治疗后,研究组D-二聚体、纤维蛋白原(FIB)含量均低于对照组,差异有统计学意义(P<0.05),研究组深静脉血栓发生率低于对照组,差异有统计学意义(P<0.05)。两组患者不良反应比较,差异无统计学意义(P>0.05)。结论LMWH联合多西他赛二线治疗驱动基因阴性的晚期NSCLC疗效较好,可以提高疾病控制率,改善凝血功能,减少静脉血栓发生。 展开更多
关键词 低分子肝素 多西他赛 非小细胞肺癌 二线化疗
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基于SSR标记的文冠果遗传多样性分析及指纹图谱构建
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作者 李思琪 张文臣 +3 位作者 杨柳 付庆新 洪新 张海旺 《生物技术通报》 CAS CSCD 北大核心 2024年第5期74-83,共10页
【目的】为快速鉴定和利用文冠果种质资源,探明文冠果种质亲缘关系。【方法】从20对SSR引物筛选得到11对条带清晰、多态性好的引物,对29个文冠果品种(系)进行分析,采用荧光毛细管电泳技术进行多态检测,通过邻接法进行聚类分析,利用数字... 【目的】为快速鉴定和利用文冠果种质资源,探明文冠果种质亲缘关系。【方法】从20对SSR引物筛选得到11对条带清晰、多态性好的引物,对29个文冠果品种(系)进行分析,采用荧光毛细管电泳技术进行多态检测,通过邻接法进行聚类分析,利用数字和字母赋值编码构建分子身份证。【结果】共检测到66个等位基因(Na),每对引物检测到3-10个Na。Shannon信息指数(I)变化范围介于0.349-1.723之间,平均值为1.16。不同引物揭示的多态信息含量(PIC)变幅介于0.276-0.841,平均为0.66。观测杂合度(Ho)变幅为0.137-0.958,平均值为0.739;期望杂合度(He)变幅为0.187-0.79,平均值为0.648;在11个位点中,有7个位点的平均观测杂合度大于平均期望杂合度。遗传相似系数变化范围为0-1,平均为0.31,遗传相似系数在0.6以上的有15个,仅占全部数据的5.17%。邻接法聚类分析结果显示,在遗传距离为0.42时,可将29份文冠果种质分为三大类群。构建了0/1形式的指纹数据库和分子身份证,除个别品种外均具有唯一性,可用于品种鉴定。【结论】29份文冠果种质资源的遗传多样性相对较高,但也存在一定的近交现象。利用SSR标记构建文冠果分子身份证操作简便可行,可为文冠果品种真伪鉴定、权益保护、身份识别、溯源管理及新品种选育提供技术支撑和科学依据。 展开更多
关键词 文冠果 品种(系) SSR荧光标记 遗传多样性 指纹图谱 分子身份证 聚类分析
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Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines for the Alleles in Rice 被引量:5
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作者 Akshay TALUKDAR 《Rice science》 SCIE 2006年第1期9-14,共6页
The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by using microsatellite class and G-T poly... The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by using microsatellite class and G-T polymorphism. Among these alleles, (CT)12-G, (CT)15-G, (CT)16-G, (CT)17-G, (CT)18-G and (CT)21-G have not been reported. Seventy-two single-segment substitution lines (SSSLs) carrying different alleles at the Wx locus were developed by using Huajingxian 74 with the (CT)11-G allele as a recipient and 20 accessions containing 12 different alleles at the Wx locus as donors. The estimated length of the substituted segments ranged from 2.2 to 77.3 cM with an average of 17.4 cM. 展开更多
关键词 RICE waxy gene single segment substitution line allelic variation molecular marker-assisted selection
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Precise measurement of the line width of the photoassociation spectra of ultracold molecules by using a frequency shifter
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作者 武寄洲 马杰 +6 位作者 姬中华 张一驰 李玉清 汪丽蓉 赵延霆 肖连团 贾锁堂 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第9期224-228,共5页
We propose a technique to precisely measure the line width of the photoassociation spectra of the excited cesium molecule by using a frequency shifter to generate two laser beams with a precise frequency difference. A... We propose a technique to precisely measure the line width of the photoassociation spectra of the excited cesium molecule by using a frequency shifter to generate two laser beams with a precise frequency difference. A series of photoassociation (PA) spectra are recorded with two laser beam induced molecular lines, whose peak separation serves as an accurate frequency ruler to measure the line width of the PA spectra. The full width half maximum line width was studied as a function of PA laser intensity. The extrapolated value at zero laser intensity is (34.8± 0.22) MHz. By analyzing other broadening mechanisms, a value of (32.02 ± 0.70) MHz was deduced. It is shown that this scheme is inexpensive, simple, robust, and is promising for applications in a variety of other atomic species. 展开更多
关键词 laser cooling molecular spectroscopy line shapes and width
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2019—2020山东小麦区试品系55个基因的 等位基因分布 被引量:1
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作者 李玮 张敏 +5 位作者 宋国琦 李玉莲 高洁 程敦公 李豪圣 李根英 《核农学报》 CAS CSCD 北大核心 2023年第1期17-26,共10页
为分析2019—2020年山东省小麦区试参试品系携带优异等位基因情况,利用产量、品质、抗性、开花期等55个基因的64个竞争性等位基因特异性聚合酶链式反应(KASP)标记对126份参试品系进行分子标记检测。结果表明,90%以上KASP标记具有较好的... 为分析2019—2020年山东省小麦区试参试品系携带优异等位基因情况,利用产量、品质、抗性、开花期等55个基因的64个竞争性等位基因特异性聚合酶链式反应(KASP)标记对126份参试品系进行分子标记检测。结果表明,90%以上KASP标记具有较好的分型结果,可高效地进行基因型鉴定。20个基因的优异等位基因频率高于80%,包括Vrn-A1、Vrn-B1、Vrn-D1、Ppd-A1、Ppd-B1、Ppd-D1、Psy-D1、GluA3g、Rht-D1、Pinb-D1、TaCwi-A1-1、TaGW2-6B、TaSus1-7B、TaGASR7-A1、1-feh-w3、TaDreb-B1、PRRA1、PRR-B1、TaFT3-B1和TaMOT1-D1;26个基因的优异等位基因频率低于30%,包括Vp1-B1、Ppo-D1、TaPds-B1、Zds-A1、TEF-7A、Lr46、Glu-B3g、TaGS5-A1、TaCwi-4A、1B/1R、Rht-B1、Lr68、TaELF3-B1、Pina-D1、Sbwm1、TaPHS1、Pm21、COMT-3B、TaCKX-D1、TaSdr-B1、Pch1、Lr34、Yr15、Fhb1、TaMoc-7A和TaGS-D1,其中TaMoc-7A、Lr34、Fhb1、Pch1、Yr15和TaSdr-B1未检测到优异等位基因;9个基因的优异等位基因频率介于30%~80%,包括Pinb2-V、TaPod-A1、Lox-B1、Glu-A1、Glu-D1、TaGS2-B1、TaGW2-6A、TaSus1-7A和Lr14a。55个基因的优异等位基因型占比呈两极分化趋势。所有参试品系中仅济农CH03、CG086和济农CH01分别携带TaCKX-D1、Pm21和COMT-3B基因的优异等位基因,可作为基因供体用于粒重、抗白粉病和茎秆木质素含量改良和品种选育。本研究明确了126份参试品系的基因型信息,基本摸清了山东参试小麦品系在重要农艺性状基因上的等位基因分布特征,为分子标记辅助选择奠定了基础。 展开更多
关键词 小麦品系 分子标记 等位基因 区域试验
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Molecular dynamics studies on spreading of nanofluids promoted by nanoparticle adsorption on solid surface 被引量:1
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作者 Fengchao Wang Hengan Wu 《Theoretical & Applied Mechanics Letters》 CAS 2013年第5期31-34,共4页
Abstract Spreading of nanofluids on solid substrate was studied via molecular dynamics simulations. Simulation models for two immiscible fluids (oil and water based nanofiuids) confined in a slit between two planar ... Abstract Spreading of nanofluids on solid substrate was studied via molecular dynamics simulations. Simulation models for two immiscible fluids (oil and water based nanofiuids) confined in a slit between two planar solid walls were set up. The influence of the volume concentration of the nanoparticles on the three-phase contact line motion was investigated. We found that the larger volume concentration results in more visible nanoparticle adsorption on solid surface. This effect further induces an advancing displacement of the contact line compared with the meniscus profiles in low concentration case and that with the absence of nanoparticles. These findings are consistent with the previous experimental and theoretical results and provide the atomic-scale understanding on nanofluid spreading. 展开更多
关键词 nanofluids three-phase contact line SPREADING nanoparticles molecular dynamics sim-ulations
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