以小麦印度腥黑穗病菌9个菌株和黑麦草腥黑穗病菌5个菌株及其近似种或相关种:稻粒黑粉菌、狼尾草腥黑粉菌、狗尾草腥黑粉菌、苏玛特腥黑粉菌、狐尾草腥黑粉菌、小麦网腥黑穗病菌和小麦矮化腥黑穗病菌共9种22个菌株为研究对象,通过序列...以小麦印度腥黑穗病菌9个菌株和黑麦草腥黑穗病菌5个菌株及其近似种或相关种:稻粒黑粉菌、狼尾草腥黑粉菌、狗尾草腥黑粉菌、苏玛特腥黑粉菌、狐尾草腥黑粉菌、小麦网腥黑穗病菌和小麦矮化腥黑穗病菌共9种22个菌株为研究对象,通过序列比对分析,设计了检测小麦印度腥黑穗病菌及黑麦草腥黑穗病菌的TaqM an MGB实时荧光PCR引物和探针,优化了反应条件,筛选出特异性探针,分别建立了小麦印度腥黑穗病菌和黑麦草腥黑穗病菌实时荧光单重PCR和实时荧光双重PCR检测方法,其中实时荧光双重PCR检测方法实现了在同一PCR管中仅用5μL的反应体系,进行1次PCR反应就能特异性检测出小麦印度腥黑穗病菌或黑麦草腥黑穗病菌。本研究所建立的检测方法特异性强、结果可靠、检测速度快、成本明显降低,在实际应用中具有推广价值。展开更多
Inflammatory cytokines are key players in modulating immune responses to mount effective host defense.However,excessive production of inflammatory cytokines contributes to the destructive components responsible for va...Inflammatory cytokines are key players in modulating immune responses to mount effective host defense.However,excessive production of inflammatory cytokines contributes to the destructive components responsible for various inflammatory disorders.As a result,treatment strategies have been developed to lower the cytokine levels or block their bioactivity.In particular,therapeutic agents that directly capture and neutralize cytokines have gained significant attention as they bypass the interactions with the host cells,and therefore,are less likely to induce immunogenic response and clearance.Among them,“monoplex”platforms such as cytokine-neutralizing antibodies(CNAs)are commonly designed to target a specific cytokine for neutralization.Meanwhile,to address the multiplexity of the cytokine targets in diseases,multiplex platforms such as glycosaminoglycan-containing biomaterials and cell-membrane-coated nanoparticles are emerging.Herein,we have reviewed the recent progress of these cytokine-neutralizing platforms(CNPs)and discussed their applications in treating inflammatory disorders.Overall,understanding the structure–function relationships underlying these CNPs would lead to the design of novel therapeutics toward effective management of inflammatory diseases.展开更多
文摘以小麦印度腥黑穗病菌9个菌株和黑麦草腥黑穗病菌5个菌株及其近似种或相关种:稻粒黑粉菌、狼尾草腥黑粉菌、狗尾草腥黑粉菌、苏玛特腥黑粉菌、狐尾草腥黑粉菌、小麦网腥黑穗病菌和小麦矮化腥黑穗病菌共9种22个菌株为研究对象,通过序列比对分析,设计了检测小麦印度腥黑穗病菌及黑麦草腥黑穗病菌的TaqM an MGB实时荧光PCR引物和探针,优化了反应条件,筛选出特异性探针,分别建立了小麦印度腥黑穗病菌和黑麦草腥黑穗病菌实时荧光单重PCR和实时荧光双重PCR检测方法,其中实时荧光双重PCR检测方法实现了在同一PCR管中仅用5μL的反应体系,进行1次PCR反应就能特异性检测出小麦印度腥黑穗病菌或黑麦草腥黑穗病菌。本研究所建立的检测方法特异性强、结果可靠、检测速度快、成本明显降低,在实际应用中具有推广价值。
基金supported by the National Science Foundation Grant DMR-1904702the Defense Threat Reduction Agency Joint Science and Technology Office for ChemicalBiological Defense under grant number HDTRA1-18-1-0014.
文摘Inflammatory cytokines are key players in modulating immune responses to mount effective host defense.However,excessive production of inflammatory cytokines contributes to the destructive components responsible for various inflammatory disorders.As a result,treatment strategies have been developed to lower the cytokine levels or block their bioactivity.In particular,therapeutic agents that directly capture and neutralize cytokines have gained significant attention as they bypass the interactions with the host cells,and therefore,are less likely to induce immunogenic response and clearance.Among them,“monoplex”platforms such as cytokine-neutralizing antibodies(CNAs)are commonly designed to target a specific cytokine for neutralization.Meanwhile,to address the multiplexity of the cytokine targets in diseases,multiplex platforms such as glycosaminoglycan-containing biomaterials and cell-membrane-coated nanoparticles are emerging.Herein,we have reviewed the recent progress of these cytokine-neutralizing platforms(CNPs)and discussed their applications in treating inflammatory disorders.Overall,understanding the structure–function relationships underlying these CNPs would lead to the design of novel therapeutics toward effective management of inflammatory diseases.
