Scientific literature addressing detection of monosialoganglioside A 10-year bibliometric analysis

OBJECTIVE： The study was undertaken to explore a bibliometric approach to quantitatively assess the research on detection of monosialoganglioside from 2002 to 2011. DATA RETRIEVAL： A bibliometric analysis based on the publications on Web of Science was performed using key words such as ＂monosialoganglioside＂, ＂colloidal gold＂, ＂high performance liquid chromatography＂ and ＂detection＂. SELECTION CRITERIA： （1） Research articles on the detection of monosialoganglioside; （2） researches on human and animal fundamentals, clinical trials and case reports; （3） article types： article, review, proceedings paper, note, letter, editorial material, discussion, book chapter; （4） Publication year： 2002-2011. Exclusion criteria： （1） unrelated articles; （2） type of articles： correction; （3） articles from following databases： all databases related to social science and arts ＆ humanities in Web of Science were excluded. MAIN OUTCOME MEASURES： （1） distribution of subject areas; （2） number of publications annually; （3） document type and language of publications; （4） distribution of institutions; （5） distribution of output in journals; （6） the number of countries in which the article is published; （7） top cited paper. RESULTS： Overall population stands at 1 880 research articles addressing detection of monosialoganglioside in Web of Science during the study period. Articles （1 599） were the most frequently used document type comprising 85.05%, followed by meeting abstracts, reviews and proceedings papers. The distribution of subject categories showed that monosialoganglioside research covered both clinical and basic science research. The USA, Japan, and Italy were the three most productive countries, and the publication numbers in the USA were highest with 559 papers. The University of Milan, Nagoya University, and Kinki University are the most productive institutions regarding detection of monosialoganglioside. In 559 articles published by Americans, Medical College of Georgia ranked the first with 30 articles, followed by University of Medicine and Dentistry of New Jersey （28 articles）, Cornell University （24 articles） and Johns Hopkins University （24 articles）. In 442 articles published by Japanese, Nagoya University ranked the first with 40 articles, followed by Kinki University （36 articles）, and Dokkyo University （31 articles）. Though the total number of publications by Japanese is smaller than Americans, the top three institutions published more publications than American institutions. There is a markedly increase in the number of publications on the subject detection of monosialoganglioside in 2004, which the peak in the past 10 years. The valley bottom of the subject appeared in 2005. In total, the research is increased with time prolonged. Journal of Neurochemistry, Journal of Biological Chemistry and Journal of Neuroimmunology were core subject journals in monosialoganglioside studies. CONCLUSION： This study highlights the topics in detection of monosialoganglioside research that are being published around the world.

Scientific literature on monosialoganglioside in the Science Citation Index-Expanded A bibliometric analysis of articles from 1942 to 2011 by each decade

BACKGROUND： The monosialoganglioside （GM1） is a popular topic of research but the bibliometric analysis of GM1 over the decades in Science Citation Index-Expanded （SCI-E） remains poorly understood. OBJECTIVE： To identify the global research and to improve the understanding of research trends in the GM1 field from 1942 to 2011. DESIGN： A bibliometric study. DATA RETRIEVAL： We performed a bibliometric analysis based on the SCI-E published by the Institute of Scientific Information. INCLUSIVE CRITERIA： Articles closely related to GM1 were included. Exclusive criteria： （1） Articles related to gangliosidosis, disialo-ganglioside, trisialo-ganglioside or ganglioside GQIb. （2） Document types such as meeting abstracts, reviews, proceedings papers, notes, and letters. MAIN OUTCOME MEASURES： （1） Type of publication output; （2） number of author outputs; （3） distribution of output in subject categories; （4） publication distribution of countries; （5） distribution of output in journals, and （6） distribution of citations in each decade. RESULTS： During 1942 to 2011, there were 10 126 papers on GM1 that were added to the SCI. Articles （8 004） were the most frequently used document type comprising 79.0%, followed by meeting abstracts, reviews and proceedings papers. Research on GM1 could be found in the SCI from 1942, it was developed in the 1970s, greatly increased in the 1980s, and reached a peak in the 1990s, and it was slightly decreased in 2000. The distribution of subject categories showed that GM1 research covered both clinical and basic science research. The USA, Japan, and Germany were the three most productive countries, and the publication numbers in the USA were highest in all decades. The Joumal of Biological Chemistry, Journal of Neurochemistry and Biochemistry were core subject journals in GM1 studies in each decade. CONCLUSION： This study highlights the topics in GM1 research that are being published around the world.

Capillary Electrophoretic Separation of Monosialogangliosides by using cyclodectrin and borax as selectors

f The CE separation of monosialogangliosids (MSGLS) is generally difficult owing to theirmixed micelle formation. The addition of high concentration of borax to the buffer containing α-Cyclodextrin allowed for improvement of selectivity of separation. Under the optimum conditions,several forms of MSGLS were successfully separated and the commercial standard GM1 gave twopeaks, suggesting that the standard may contain two different types of species.

Enhancing Effect of Monosialoganglioside GM1 on Learning and Memory in Aged Rats

Introduction Gangliosides are found at high concentration in CNS, and are essentially combined with neuronal membrane. The chemical structures of gangliosides have been determined. They are a family of molecules consisting of a lipid portion anchored in the plasma membrane and a carbohydrate portion on the outer cell surface containing one or more sialic acid residues. The hydrophihc carbohydrate portion is related to the regulation of Ca2+ channel. Exogenous ganglioside administration may have facilitatory effects on

The Role of Monosialoganglioside GM1 in LTP-Induction in Rat Hippocampal Slices

The effect of monosialoganglioside GM1 of different doses on the long-term potentiation (LTP) of synaptic transmission has been studied in the CA1 region of rat hippocampal slices, and the possible role that calcium ion and NMDA receptor play has also been investigated. The results reveal that larger magnitude of LTP is induced in hippocampal slices pre-incubated with GM1. The dose-response curve appears in diphase, and the largest magnitude of LTP has been obtained at the GM1 concentration of 50 mg/L in incubation ACSF. Moreover, the magnitude of LTP induced from the slices pre-incubated with GM1 at lower calcium ion concentration is similar to that obtained from the control slices at normal calcium ion concentration. Under higher calcium ion concentration, the enhancing effect of GM1 on LTP seems relatively feeble. After NMDA receptors were blocked, no enhancing effect of GM1 was observed. The mechanism of GM1 action on LTP is discussed.

Observation on the therapeutic effect of aspirin in combined with ozagrel sodium in the treatment of acute cerebral infarction

Objective:To evaluate the changes in serum index levels after monosialoganglioside injection combined with conventional treatment of neonatal hypoxic ischemic encephalopathy. Methods:A total of 70 children with neonatal hypoxic ischemic encephalopathy treated in our hospital between February 2013 and February 2016 were selected and randomly divided into observation group and control group, control group received clinical routine treatment and observation group accepted monosialoganglioside injection combined with conventional treatment. After 1 week of treatment, serum levels of apoptosis factors, nerve function indexes, oxidation/anti-oxidation indexes and disease severity indexes of two groups of patients were detected.Results:Serum PDCD5, sFas, sFasL, NSE, S100-β, MDA, NO, NOS, H-FABP, NPY, caspase-1 and ET-1 levels of observation group were lower than those of control group while BDNF, NGF, SOD, GSH-PX, IGF-1 and GH levels were higher than those of control group.Conclusion: Monosialoganglioside injection can enhance the overall treatment effect and promote the realization of homeostasis in children with HIE.

Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase Ⅱ in spinal cord injury rats

Electroacupuncture is beneficial for the recovery of spinal cord injury, but the underlying mechanism is unclear. The Rho/Rho-associated kinase（ROCK） signaling pathway regulates the actin cytoskeleton by controlling the adhesive and migratory behaviors of cells that could inhibit neurite regrowth after neural injury and consequently hinder the recovery from spinal cord injury. Therefore, we hypothesized electroacupuncture could affect the Rho/ROCK signaling pathway to promote the recovery of spinal cord injury. In our experiments, the spinal cord injury in adult Sprague-Dawley rats was caused by an impact device. Those rats were subjected to electroacupuncture at Yaoyangguan（GV3）, Dazhui（GV14）, Zusanli（ST36） and Ciliao（BL32） and/or monosialoganglioside treatment. Behavioral scores revealed that the hindlimb motor functions improved with those treatments. Real-time quantitative polymerase chain reaction, fluorescence in situ hybridization and western blot assay showed that electroacupuncture suppressed the m RNA and protein expression of Rho-A and Rho-associated kinase Ⅱ（ROCKⅡ） of injured spinal cord. Although monosialoganglioside promoted the recovery of hindlimb motor function, monosialoganglioside did not affect the expression of Rho-A and ROCKⅡ. However, electroacupuncture combined with monosialoganglioside did not further improve the motor function or suppress the expression of Rho-A and ROCKⅡ. Our data suggested that the electroacupuncture could specifically inhibit the activation of the Rho/ROCK signaling pathway thus partially contributing to the repair of injured spinal cord. Monosialoganglioside could promote the motor function but did not suppress expression of Rho A and ROCKⅡ. There was no synergistic effect of electroacupuncture combined with monosialoganglioside.