Objective:To evaluate the phytochemical and anti-bacterial potential of mother plants in vivo and in vitro derived callus of Baliospermum montanum(B.montanum)(Willd.) Muell.-Arg. leaves and root.Methods:The in vitro d...Objective:To evaluate the phytochemical and anti-bacterial potential of mother plants in vivo and in vitro derived callus of Baliospermum montanum(B.montanum)(Willd.) Muell.-Arg. leaves and root.Methods:The in vitro derived rootlets and leaves segments of B.montanum were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-D either alone or in combinations.The preliminary phytochemical screening was performed by Harborne method.Antibacterial efficacy was performed by well diffusion method and incubated for 24 h at 37℃.Results:The highest percentage of callus formation(leaves segments 86.9±0.56:root segments 78.7±0.51) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 2.0 mg/L of 2.4-Dichlorophenoxy acetic acid.The phytochemical study revealed the high quantity presence of steroids,triterpenoids,glycosides,saponins,alkaloids,flavanoids, phenolic compounds,tannins,sugars etc of root and leaves derived calli.The ethanol extract of leaves segment derived calli of B.montanum showed the maximum solubility and antimicrobial activity with the MIC ranged from 100 to 200μL.Conclusions:The preliminary phytochemical study confirmed that the calli mediated tissues showed the higher percentage of metabolite constituents and extraction value compared to the in vivo leaves and roots.The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds.展开更多
Objective:To investigate the effects of Gymnema montanum leaf extract against endoplasmic reticulum(ER)stress-induced toxicity in endothelial cells.Methods:The immortalized endothelial hybrid cell,EA.hy926 was treated...Objective:To investigate the effects of Gymnema montanum leaf extract against endoplasmic reticulum(ER)stress-induced toxicity in endothelial cells.Methods:The immortalized endothelial hybrid cell,EA.hy926 was treated with different concentrations of Gymnema montanum leaf extract(0–100μg/mL)and the ER stress inducer,tunicamycin.The cytotoxicity was assessed by MTT as well as lactate dehydrogenase and malondialdehyde levels were determined.The levels of ER stress markers,GRP78 and CHOP were analysed by Western blot assay.The Gymnema montanum leaf extract-mediated activation of nuclear factor erythroid 2-related factor 2(Nrf2)was assessed by cell-based luciferase enzyme fragment complementation assay and antioxidant responsive element driven luciferase reporter assay.The levels of phosphoproteins of the MAPK pathway were analyzed using the Bioplex system.Results:A dose-dependent cytoprotective effect of Gymnema montanum leaf extract was observed in tunicamycin-induced toxicity.Gymnema montanum leaf extract significantly reduced lactate dehydrogenase activity and malondialdehyde levels in ER stress-induced endothelial cells.It also suppressed ER stress markers dose dependently and inhibited the phosphorylation of JNK,ERK,MEK and p38 MAPK in tunicamycin-induced endothelial cells.Moreover,Gymnema montanum leaf extract increased the expression of Nrf2 and its downstream targets in endothelial cells.Conclusions:Gymnema montanum leaf extract attenuates ER stress by increasing the expression of Nrf2 and its downstream genes.展开更多
A pair of dimeric stilbenes, gnetifolin M and gnetifolin N were isolated from the lianas of Gnetum montanum. All attempts to separate them by HPLC were unsuccessful. Their str-uctures were elucidated by the spectrosco...A pair of dimeric stilbenes, gnetifolin M and gnetifolin N were isolated from the lianas of Gnetum montanum. All attempts to separate them by HPLC were unsuccessful. Their str-uctures were elucidated by the spectroscopic analysis including 2D-NMR studies on the peracetate.展开更多
Two new dimeric stilbenes, named gnetifolin L and gnetifolin O, were isolated from the alcoholic extract of lianas of Gnetum montanum. Their structures were elucidated mainly on the basis of spectroscopic data includi...Two new dimeric stilbenes, named gnetifolin L and gnetifolin O, were isolated from the alcoholic extract of lianas of Gnetum montanum. Their structures were elucidated mainly on the basis of spectroscopic data including 2D-NMR studies on peracetate derivatives.展开更多
光果姜[Zingiber montanum(J.Koenig)Link ex A.Dietr]为姜科姜属多年生草本植物,具有殷红的锥形花序,极具观赏价值,其花序轴较短且柔软易断,可通过杂交手段进行品种改良。此外,姜科植物的系统进化关系一直存在争议,目前关于光果姜生殖...光果姜[Zingiber montanum(J.Koenig)Link ex A.Dietr]为姜科姜属多年生草本植物,具有殷红的锥形花序,极具观赏价值,其花序轴较短且柔软易断,可通过杂交手段进行品种改良。此外,姜科植物的系统进化关系一直存在争议,目前关于光果姜生殖生物学研究相对缺乏。为了促进杂交育种工作的顺利进行以及为姜科植物系统进化提供新的证据,该研究以石蜡切片法为主要研究方法,进一步结合整体压片法和半薄切片法,观察了姜科姜属植物光果姜的大小孢子和雌雄配子体的发育过程,旨在深入了解光果姜的生殖生物学解剖学特征,为姜科植物保育工作提供理论依据。结果表明:①光果姜花药包含2室,花药壁的形成方式属于基本型,具有变形绒毡层;②小孢子母细胞减数分裂类型为连续型,四分体包含左右对称型,直线型和T型,成熟花粉粒为2-细胞型;③胚珠的类型为倒生胚珠,具有双珠被和厚珠心,雌配子体发育类型为蓼型;④揭示了雌雄配子体发育的对应关系,二者成熟时间同步,但雄蕊进入减数分裂时期比雌蕊早。首次发现光果姜花药壁中的绒毡层类型为变形绒毡层,这与前人报道的其他姜科植物的特征不同。研究结果可为姜科植物繁育工作及其系统分类提供有力的科学依据。展开更多
文摘Objective:To evaluate the phytochemical and anti-bacterial potential of mother plants in vivo and in vitro derived callus of Baliospermum montanum(B.montanum)(Willd.) Muell.-Arg. leaves and root.Methods:The in vitro derived rootlets and leaves segments of B.montanum were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-D either alone or in combinations.The preliminary phytochemical screening was performed by Harborne method.Antibacterial efficacy was performed by well diffusion method and incubated for 24 h at 37℃.Results:The highest percentage of callus formation(leaves segments 86.9±0.56:root segments 78.7±0.51) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 2.0 mg/L of 2.4-Dichlorophenoxy acetic acid.The phytochemical study revealed the high quantity presence of steroids,triterpenoids,glycosides,saponins,alkaloids,flavanoids, phenolic compounds,tannins,sugars etc of root and leaves derived calli.The ethanol extract of leaves segment derived calli of B.montanum showed the maximum solubility and antimicrobial activity with the MIC ranged from 100 to 200μL.Conclusions:The preliminary phytochemical study confirmed that the calli mediated tissues showed the higher percentage of metabolite constituents and extraction value compared to the in vivo leaves and roots.The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds.
基金supported by the Indian Council of Medical Research(grant no.59/57/2011/BMS/TRM),Government of India
文摘Objective:To investigate the effects of Gymnema montanum leaf extract against endoplasmic reticulum(ER)stress-induced toxicity in endothelial cells.Methods:The immortalized endothelial hybrid cell,EA.hy926 was treated with different concentrations of Gymnema montanum leaf extract(0–100μg/mL)and the ER stress inducer,tunicamycin.The cytotoxicity was assessed by MTT as well as lactate dehydrogenase and malondialdehyde levels were determined.The levels of ER stress markers,GRP78 and CHOP were analysed by Western blot assay.The Gymnema montanum leaf extract-mediated activation of nuclear factor erythroid 2-related factor 2(Nrf2)was assessed by cell-based luciferase enzyme fragment complementation assay and antioxidant responsive element driven luciferase reporter assay.The levels of phosphoproteins of the MAPK pathway were analyzed using the Bioplex system.Results:A dose-dependent cytoprotective effect of Gymnema montanum leaf extract was observed in tunicamycin-induced toxicity.Gymnema montanum leaf extract significantly reduced lactate dehydrogenase activity and malondialdehyde levels in ER stress-induced endothelial cells.It also suppressed ER stress markers dose dependently and inhibited the phosphorylation of JNK,ERK,MEK and p38 MAPK in tunicamycin-induced endothelial cells.Moreover,Gymnema montanum leaf extract increased the expression of Nrf2 and its downstream targets in endothelial cells.Conclusions:Gymnema montanum leaf extract attenuates ER stress by increasing the expression of Nrf2 and its downstream genes.
文摘A pair of dimeric stilbenes, gnetifolin M and gnetifolin N were isolated from the lianas of Gnetum montanum. All attempts to separate them by HPLC were unsuccessful. Their str-uctures were elucidated by the spectroscopic analysis including 2D-NMR studies on the peracetate.
文摘Two new dimeric stilbenes, named gnetifolin L and gnetifolin O, were isolated from the alcoholic extract of lianas of Gnetum montanum. Their structures were elucidated mainly on the basis of spectroscopic data including 2D-NMR studies on peracetate derivatives.
文摘光果姜[Zingiber montanum(J.Koenig)Link ex A.Dietr]为姜科姜属多年生草本植物,具有殷红的锥形花序,极具观赏价值,其花序轴较短且柔软易断,可通过杂交手段进行品种改良。此外,姜科植物的系统进化关系一直存在争议,目前关于光果姜生殖生物学研究相对缺乏。为了促进杂交育种工作的顺利进行以及为姜科植物系统进化提供新的证据,该研究以石蜡切片法为主要研究方法,进一步结合整体压片法和半薄切片法,观察了姜科姜属植物光果姜的大小孢子和雌雄配子体的发育过程,旨在深入了解光果姜的生殖生物学解剖学特征,为姜科植物保育工作提供理论依据。结果表明:①光果姜花药包含2室,花药壁的形成方式属于基本型,具有变形绒毡层;②小孢子母细胞减数分裂类型为连续型,四分体包含左右对称型,直线型和T型,成熟花粉粒为2-细胞型;③胚珠的类型为倒生胚珠,具有双珠被和厚珠心,雌配子体发育类型为蓼型;④揭示了雌雄配子体发育的对应关系,二者成熟时间同步,但雄蕊进入减数分裂时期比雌蕊早。首次发现光果姜花药壁中的绒毡层类型为变形绒毡层,这与前人报道的其他姜科植物的特征不同。研究结果可为姜科植物繁育工作及其系统分类提供有力的科学依据。
