期刊文献+
共找到309篇文章
< 1 2 16 >
每页显示 20 50 100
Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells 被引量:1
1
作者 Tong Wu Fu Zheng +7 位作者 Hong-Yi Tang Hua-Zhi Li Xin-Yu Cui Shuai Ding Duo Liu Cui-Ying Li Jiu-Hui Jiang Rui-Li Yang 《World Journal of Stem Cells》 SCIE 2024年第3期267-286,共20页
BACKGROUND The bone remodeling during orthodontic treatment for malocclusion often requires a long duration of around two to three years,which also may lead to some complications such as alveolar bone resorption or to... BACKGROUND The bone remodeling during orthodontic treatment for malocclusion often requires a long duration of around two to three years,which also may lead to some complications such as alveolar bone resorption or tooth root resorption.Low-intensity pulsed ultrasound(LIPUS),a noninvasive physical therapy,has been shown to promote bone fracture healing.It is also reported that LIPUS could reduce the duration of orthodontic treatment;however,how LIPUS regulates the bone metabolism during the orthodontic treatment process is still unclear.AIM To investigate the effects of LIPUS on bone remodeling in an orthodontic tooth movement(OTM)model and explore the underlying mechanisms.METHODS A rat model of OTM was established,and alveolar bone remodeling and tooth movement rate were evaluated via micro-computed tomography and staining of tissue sections.In vitro,human bone marrow mesenchymal stem cells(hBMSCs)were isolated to detect their osteogenic differentiation potential under compression and LIPUS stimulation by quantitative reverse transcription-polymerase chain reaction,Western blot,alkaline phosphatase(ALP)staining,and Alizarin red staining.The expression of Yes-associated protein(YAP1),the actin cytoskeleton,and the Lamin A/C nucleoskeleton were detected with or without YAP1 small interfering RNA(siRNA)application via immunofluorescence.RESULTS The force treatment inhibited the osteogenic differentiation potential of hBMSCs;moreover,the expression of osteogenesis markers,such as type 1 collagen(COL1),runt-related transcription factor 2,ALP,and osteocalcin(OCN),decreased.LIPUS could rescue the osteogenic differentiation of hBMSCs with increased expression of osteogenic marker inhibited by force.Mechanically,the expression of LaminA/C,F-actin,and YAP1 was downregulated after force treatment,which could be rescued by LIPUS.Moreover,the osteogenic differentiation of hBMSCs increased by LIPUS could be attenuated by YAP siRNA treatment.Consistently,LIPUS increased alveolar bone density and decreased vertical bone absorption in vivo.The decreased expression of COL1,OCN,and YAP1 on the compression side of the alveolar bone was partially rescued by LIPUS.CONCLUSION LIPUS can accelerate tooth movement and reduce alveolar bone resorption by modulating the cytoskeleton-Lamin A/C-YAP axis,which may be a promising strategy to reduce the orthodontic treatment process. 展开更多
关键词 Low-intensity pulsed ultrasound Bone resorption OSTEOGENESIS Cytoskeleton-Lamin A/C-Yes-associated protein axis Bone marrow mesenchymal stem cells Orthodontic tooth movement
下载PDF
Virus Movement Protein Gene Mediated Resistance Against Cucumber Mosaic Virus Infection 被引量:6
2
作者 张振臣 李大伟 +2 位作者 张力 于嘉林 刘仪 《Acta Botanica Sinica》 CSCD 1999年第6期585-590,共6页
Tobacco ( Nicotiana tabacum L.) “NC89” plants were transformed with deletion mutant of cucumber mosaic virus (CMV) movement protein (MP) gene and full_length CMV MP gene, respectively. The transformed plants... Tobacco ( Nicotiana tabacum L.) “NC89” plants were transformed with deletion mutant of cucumber mosaic virus (CMV) movement protein (MP) gene and full_length CMV MP gene, respectively. The transformed plants were analyzed with polymerase chain reaction (PCR), PCR_Southern, Southern and Western blots. R 0 generation of the transgenic plants were inoculated with CMV. Five out of 10 lines of tobacco plants (BMPK) transformed with CMV MP deletion mutant gene showed high resistance to CMV infection and remained symptomless for up to 50 days post_inoculation. In contrast, tobacco plants (BMPR) transformed with full_length CMV MP gene did not show resistance to CMV infection. However, most of the infected full_length CMV MP gene transgenic plants recovered by showing none or very mild mosaic symptoms in 40 days post_inoculation. The results of R 1 generation of the BMPK transgenic plants tested under field conditions showed that all 5 lines of transgenic plants could delay the virus disease development. 展开更多
关键词 Cucumber mosaic virus movement protein gene Transgenic plants RESISTANCE
下载PDF
Expression and Purification of Recombinant MP-GFP Protein in Escherichia coli 被引量:1
3
作者 CHEN Xiao-jun XU Han-hong 《Agricultural Sciences in China》 CAS CSCD 2011年第3期394-403,共10页
Guided pesticide is an unique compound resulted from the conjugation with carrier (amino acid, protein, sugars, etc) and the active pesticide ingredient. One of the attributes of the guided pesticide is its potentia... Guided pesticide is an unique compound resulted from the conjugation with carrier (amino acid, protein, sugars, etc) and the active pesticide ingredient. One of the attributes of the guided pesticide is its potential to accumulate at the site of the damaged points caused by pest or at the site of entry to the target pests, such as via inhalation, cuticular penetration, and oral digestion. Movement protein (MP) is a kind of protein coded by plant virus. A genetic fusion between green fluorescent protein (GFP) and movement protein resulted in the expression of a fluorescent fusion MP-GFP protein, which was fully biologically active in mediating the cell-to-cell spread of virus. In order to obtain a suitable carrier for a pesticide, fluorescent carrier MP-GFP was constructed. It was found that the recombinant MP-GFP protein was the inclusion body. The results indicated that optimized cultural condition for expression of recombinant MP-GFP protein was incubation at 37°C for 2 h and induction with 0.2 mmol L-1 IPTG (isopropyl-b-dthiogalactopyranoside) at 25°C for 4 h. MP-GFP protein was purified by using Ni-NTA resin. The expressed recombinant MP-GFP protein had both the fluorescence character of report GFP gene and moving character of movement protein. It could provide a guided carrier for studying the guided pesticide. It could also provide convenience for studying the delivery and distribution of the guided pesticide ingredients in the plant. 展开更多
关键词 movement protein green fluorescent protein PURIFICATION
下载PDF
P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat protein 被引量:1
4
作者 RUI Peng-huan WANG Zhan-qi +5 位作者 SHAN Wen-shu XIA Wei-wei ZHOU Xiu-hong YANG Lian-lian JIANG Lei JIANG Tong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第4期1071-1083,共13页
Although the complete nucleotide sequence of strawberry vein banding virus(SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of ea... Although the complete nucleotide sequence of strawberry vein banding virus(SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of each protein is unclear. This study provided evidence that the P1 protein of SVBV(SVBV-P1) possesses the following features. Bioinformatic and subcellular localization analyses showed that SVBV-P1 is localized in the cytoplasm and cell walls of epidermal cells in Nicotiana benthamiana, and it forms inclusion bodies associated with microtubules and the endoplasmic reticulum. Dilution experiments demonstrated that SVBV-P1 could move from the original agro-infiltrated cells to adjacent cells in N. benthamiana leaves. Further trans-complementation experiments demonstrated that SVBV-P1 could facilitate the intercellular movement of a movement-deficient potato virus X mutant in N. benthamiana leaves. Finally, yeast twohybrid and bimolecular fluorescence complementation assays revealed that SVBV-P1 could interact with the SVBV coat protein, which is a major component of Caulimovirus virions. Results of the electrophoretic mobility shift assay indicated that SVBV-P1 lacks DNA-binding capability. In summary, the results suggest that SVBV-P1 is probably a movement protein of SVBV, providing new insights into the function of movement proteins of the Caulimovirus genus. 展开更多
关键词 strawberry vein banding virus P1 protein movement protein coat protein virus movement
下载PDF
Cloning and Sequence Analysis of the 28.5ku Movement Protein of Frangipani Mosaic Virus (FMV)
5
作者 DENG Xiao-dong FEI Xiao-wen LIU Zhi-xin HU Xin-wen ZHENG Xue-qin 《Agricultural Sciences in China》 CAS CSCD 2001年第1期148-150,共3页
Based on conserved regions among genomic RNA of tobamoviruses, a pair of primers spanning the sequence encoding the movement protein were synthesized. A cDNA fragment of 1700bp was thus amplified by RT-PCR(reverse tra... Based on conserved regions among genomic RNA of tobamoviruses, a pair of primers spanning the sequence encoding the movement protein were synthesized. A cDNA fragment of 1700bp was thus amplified by RT-PCR(reverse transcription-polymerase chain reaction). The fragment was cloned into pGEM-T easy vector and sequenced. DNA sequence analysis showed that the fragment contained a region of 768 nucleotides encoding protein of 256 amino acid of frangipani mosaic virus (FMV) and also partial sequence corresponding to 180ku and 17. 5ku protein. 展开更多
关键词 Plumeria acutifolia FMV movement protein GENE SEQUENCE ANALYSIS
下载PDF
Effects of Sodium Diclofenac on the Distribution of Fos Protein in Central Amygdala and Lateral Hypothalamus during Experimental Tooth Movement in Rats
6
作者 Ana Paula R. Novaes Amanda C. Desiderá +1 位作者 Glauce C. Nascimento Christie R. A. Leite-Panissi 《World Journal of Neuroscience》 2014年第2期183-189,共7页
This study evaluated whether the administration of a NSAID, sodium diclofenac, can promote alterations in the expression of Fos protein in central amygdala (CEA) and the lateral hypothalamus (LH) after 6 h of experime... This study evaluated whether the administration of a NSAID, sodium diclofenac, can promote alterations in the expression of Fos protein in central amygdala (CEA) and the lateral hypothalamus (LH) after 6 h of experimental tooth movement with a controlled force of 70 g, applied to the superior central incisors of rats. Adult male rats were anesthetized and divided into four groups: Control, no orthodontic appliance (OA);OA activated with 70 g;OA activated with 70 g and pretreated with diclofenac sodium (5 mg/kg, intramuscular);and diclofenac sodium alone. Six hours after the onset of the experiment the rats were reanesthetized and perfused with 4% paraformaldehyde. The brains were removed and fixed, and sections containing the CEA and LH were processed for Fos protein immunohistochemistry. The results show that in the control group, intramuscular injection of a ketamine/xylazine mixture did not induce IR-Fos cells in the CEA or LH. However, in the 70 g group, IR-Fos was the strongest observed 展开更多
关键词 AMYGDALA LATERAL HYPOTHALAMUS FOS protein ORTHODONTIC movement Sodium DICLOFENAC
下载PDF
Identification of key residues in protein functional movements by using molecular dynamics simulations combined with a perturbation-response scanning method
7
作者 Jun-Bao Ma Wei-Bu Wang Ji-Guo Su 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第10期665-672,共8页
The realization of protein functional movement is usually accompanied by specific conformational changes,and there exist some key residues that mediate and control the functional motions of proteins in the allosteric ... The realization of protein functional movement is usually accompanied by specific conformational changes,and there exist some key residues that mediate and control the functional motions of proteins in the allosteric process.In the present work,the perturbation-response scanning method developed by our group was combined with the molecular dynamics(MD)simulation to identify the key residues controlling the functional movement of proteins.In our method,a physical quantity that is directly related to protein specific function was introduced,and then based on the MD simulation trajectories,the perturbation-response scanning method was used to identify the key residues for functional motions,in which the residues that highly correlated with the fluctuation of the function-related quantity were identified as the key residues controlling the specific functional motions of the protein.Two protein systems,i.e.,the heat shock protein 70 and glutamine binding protein,were selected as case studies to validate the effectiveness of our method.Our calculated results are in good agreement with the experimental results.The location of the key residues in the two proteins are similar,indicating the similar mechanisms behind the performance of their biological functions. 展开更多
关键词 protein functional movements molecular dynamics simulations perturbation-response scanning method
下载PDF
不同剂量利塞膦酸钠对正畸大鼠牙根组织中FAK、MMP-2、RANK蛋白表达及牙移动、牙根吸收的影响 被引量:1
8
作者 支方静 刘振霞 白琴 《临床和实验医学杂志》 2023年第3期233-237,共5页
目的探讨不同剂量利塞膦酸钠对正畸大鼠牙根组织中FAK、基质金属蛋白酶2(MMP-2)、RANK蛋白表达及牙移动、牙根吸收的影响。方法选择120只SPF级的Wistar雄性大鼠,将120只大鼠按随机数字表法分为4组,包括模型组、利塞膦酸钠低剂量组、中... 目的探讨不同剂量利塞膦酸钠对正畸大鼠牙根组织中FAK、基质金属蛋白酶2(MMP-2)、RANK蛋白表达及牙移动、牙根吸收的影响。方法选择120只SPF级的Wistar雄性大鼠,将120只大鼠按随机数字表法分为4组,包括模型组、利塞膦酸钠低剂量组、中剂量组、高剂量组,每组30只大鼠。高、中、低利塞膦酸钠的每日给药剂量为1、0.5、0.25 mg·kg^(-1)·d^(-1),模型组大鼠在相同时间给予0.9%氯化钠溶液灌胃。分析4组大鼠不同时间点的正畸牙移动距离、4组大鼠牙根组织形态、牙根组织中破骨细胞的数量、牙根组织中MMP-2、RANK及FAK蛋白表达。结果实验第7、14、21天,高剂量组的正畸牙移动距离、破骨细胞数量、明显较中、低剂量组、模型组大鼠低,中剂量明显较低剂量组、模型组低,低剂量组明显较模型组低,差异均有统计学意义(P<0.05)。苏木素和伊红染色后,实验第7天,模型组根分叉压力侧表面的牙骨质不连续,存在骨吸收陷窝,低、中剂量组也出现骨吸收陷窝,高剂量组骨吸收陷窝较浅;实验第14天,模型组的根分叉处、根尖处均有一定吸收,低、中剂量组牙根吸收多出现在受压力侧的根中1/3处,高剂量组大鼠根尖、根分叉形态基本完整,无明显的吸收;实验第21天,模型组的根尖受压力侧出现轻度吸收,低、中、高剂量组未出现根尖形态明显变化。实验第7、14、21天,高剂量组的牙根组织中MMP-2、RANK蛋白表达明显较中、低剂量组、模型组大鼠低,FAK蛋白明显高,中剂量组正畸牙根组织中MMP-2、RANK蛋白表达明显较低剂量组、模型组低,FAK蛋白明显高,低剂量组MMP-2、RANK蛋白表达明显较模型组低,FAK蛋白明显高,差异均有统计学意义(P<0.05)。结论利塞膦酸钠可抑制正畸大鼠的牙根吸收的影响,可能与抑制牙根组织中MMP-2、RANK蛋白表达,诱导FAK蛋白升高,抑制破骨细胞数量有关,且利塞膦酸钠剂量越高,抑制牙根吸收作用越明显。 展开更多
关键词 大鼠 利塞膦酸钠 正畸 牙根组织 牙根吸收 牙齿移动 FAK蛋白表达
下载PDF
CDC20在肺腺癌组织中的表达及对肺腺癌细胞增殖和侵袭的影响研究 被引量:1
9
作者 周雪芹 栾艳超 +2 位作者 赵莉 戎超超 杨娜 《中国癌症杂志》 CAS CSCD 北大核心 2024年第5期460-472,共13页
背景与目的:肺腺癌具有早期发现难、肿瘤进展快及晚期手术切除率低等特点。尽管单药免疫治疗和免疫治疗联合化疗的相关研究在改善预后、克服耐药方面已初显成效,但是大部分肺腺癌患者从中获益仍有限。因此,迫切需要寻找具有相对较高灵... 背景与目的:肺腺癌具有早期发现难、肿瘤进展快及晚期手术切除率低等特点。尽管单药免疫治疗和免疫治疗联合化疗的相关研究在改善预后、克服耐药方面已初显成效,但是大部分肺腺癌患者从中获益仍有限。因此,迫切需要寻找具有相对较高灵敏度和特异度的新型生物标志物,以改善肺腺癌患者的预后。细胞分裂周期蛋白20(cell division cycle protein 20,CDC20)参与多种肿瘤的发生、发展,但在肺腺癌中的生物学作用及机制尚未明确。本研究旨在探究CDC20在肺腺癌中的表达情况及其对肺腺癌患者预后的预测价值,并分析CDC20对肺腺癌细胞增殖和侵袭能力的影响。方法:采用免疫组织化学(immunohistochemistry,IHC)检测CDC20在肺腺癌中的表达情况并结合生物信息学和临床病理学参数分析其与预后不良的相关性。采用Kaplan-Meier生存曲线描述CDC20对肺腺癌患者术后生存率的影响,采用COX多因素回归分析影响肺腺癌患者术后生存率的独立预后因素。通过受试者工作特征曲线分析CDC20表达在肺腺癌患者中的诊断价值。采用实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RTFQ-PCR)和蛋白质印迹法(Western blot)检测人正常肺上皮细胞系BEAS-2B、人肺腺癌细胞系A549和H1299中CDC20的表达水平。细胞实验中,通过敲低肺腺癌细胞中的CDC20,分为si-NC(对照组)、si-CDC20#1(敲低组1)和si-CDC20#2(敲低组2)3个组。采用细胞计数试剂盒-8(cell counting kit-8,CCK-8)、克隆形成、transwell和划痕实验检测细胞增殖、迁移和侵袭能力。通过基因本体论(Gene Ontology,GO)功能和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析CDC20在肺腺癌中的生物学作用。通过基因集富集分析(gene set enrichment analysis,GSEA)CDC20在肺腺癌中可能的调控通路。本研究经河北省胸科医院伦理委员会批准(编号:2022051)。结果:生物信息学及IHC结果均显示,CDC20在肺腺癌组织中显著高表达(P<0.05)。生物信息学与临床参数分析结果均显示,CDC20高表达与患者预后不良相关。Kaplan-Meier生存分析和COX回归分析均显示,CDC20表达情况与患者术后生存率呈显著负相关(P<0.05)。敲低CDC20能抑制肺腺癌细胞增殖、迁移和侵袭(P<0.05)。GO功能、KEGG通路和GSEA结果均显示,CDC20与细胞周期相关。结论:CDC20在肺腺癌中高表达,CDC20高表达是肺腺癌患者不良预后的独立危险因素。CDC20能促进肺腺癌细胞增殖、迁移和侵袭。 展开更多
关键词 细胞分裂周期蛋白20 肺腺癌 细胞增殖 细胞周期 细胞运动
下载PDF
Transient Expression of BYDV-MP in Nicotiana benthamiana 被引量:5
10
作者 王媛媛 刘国富 +1 位作者 李芳芳 曹雪松 《Agricultural Science & Technology》 CAS 2010年第1期99-102,共4页
[Objective]The aim of this study was to identify transient expression of movement protein (MP) gene in Nicotinana benthaminana rapidly and further investigate the function of this exogenous gene. [Method]The movemen... [Objective]The aim of this study was to identify transient expression of movement protein (MP) gene in Nicotinana benthaminana rapidly and further investigate the function of this exogenous gene. [Method]The movement protein gene of barley yellow dwarf virus (BYDV) was cloned into potato virus X (PVX) viral vector of pGR107,and PVX-recombinant vector was obtained. After electroporation of Agrobacterium tumefaciens,PVX was inoculated into the lower leaves of tobacco by Agrobacterium infiltration assay to observe the infection of virus on tobacco. [Result]After infection for 7 days,upper non-inoculated leaves of tobacco infected by the PVX-recombinant vector showed the virus infection symptoms,while the control group had no viral infection phenomenon. Daily follow-up observations for two groups revealed that tobacco infected by PVX-recombinant vector had severe symptoms of virus infection and curling leaves,or even led to necrosis both in infiltrated and systemic leaves in late period. However,tobacco infected by PVX vector had only slight symptoms of virus infection and could recover from infection. RT-PCR of the infected tobacco indicated that exogenous gene BYDV-MP had a normal transcription and expression in tobacco. [Conclusion]As a determinant factor for viral disease,BYDV-MP promotes the systemic infection rate of PVX and its symptom. In addition,it is feasible to express exogenous MP gene in Nicotiana benthaminan via PVX expression vector. 展开更多
关键词 movement protein of barley yellow dwarf virus (BYDV-mp Potato virus X (PVX) Nicotiana benthamiana Inoculate
下载PDF
RMP基因沉默对肝癌SMMC-7721细胞增殖和迁移能力的影响 被引量:4
11
作者 连晓宁 杨慧翠 +5 位作者 曹锴 李敏 盛伟华 王晓婷 郭云兰 魏文祥 《肿瘤》 CAS CSCD 北大核心 2010年第1期15-20,共6页
目的:建立RMP(RPB5-mediating protein)基因沉默的稳定细胞株,研究RMP小干扰RNA(small interfering RNA,siRNA)对人肝癌SMMC-7721细胞增殖和迁移的抑制作用。方法:首先,体外合成3条RMP基因的siRNA,并转染到SMMC-7721细胞中,RT-PCR检测3... 目的:建立RMP(RPB5-mediating protein)基因沉默的稳定细胞株,研究RMP小干扰RNA(small interfering RNA,siRNA)对人肝癌SMMC-7721细胞增殖和迁移的抑制作用。方法:首先,体外合成3条RMP基因的siRNA,并转染到SMMC-7721细胞中,RT-PCR检测3条siRNA对RMP基因表达的抑制作用,筛选出最佳干扰序列;然后,pGPU6-Neo-RMP-484真核表达载体转染,G418筛选出稳定干扰RMP基因的细胞株,RT-PCR法检测该稳定细胞株中RMP基因的干扰效率,MTT法检测RMP-siRNA对SMMC-7721细胞增殖及细胞黏附能力的影响,划痕实验检测细胞迁移能力的变化。结果:利用RNA干扰技术成功建立了RMP基因下调的稳定细胞株,与SMMC-7721细胞对照组比较,其RMP mRNA表达水平下调了(83.67±2.56)%。稳定转染siRNA的细胞株细胞增殖受到抑制,增殖抑制率可达(74.33±0.58)%。稳定转染细胞株的细胞黏附能力有所增加,迁移率则相应降低。结论:成功筛选出了稳定转染RMP-siRNA重组载体的细胞株。pGPU6-Neo-RMP-484重组载体能有效抑制RMP基因在肝癌SMMC-7721细胞内的表达,可作为RMP分子机制研究的细胞模型。RMP基因的下调能有效抑制肝癌细胞SMMC-7721的增殖,使其黏附率增加,同时细胞的迁移率降低。 展开更多
关键词 肝细胞 RNA干扰 细胞增殖 细胞运动 RPB5调节蛋白
下载PDF
胃癌抗原相关基因MPS-1的融合表达及兔抗GST-MPS-1抗体的制备 被引量:1
12
作者 王运伟 朱正纲 +4 位作者 顾琴龙 李建芳 瞿颖 刘炳亚 林言箴 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2006年第1期60-63,共4页
目的在大肠杆菌中表达GST-MPS-1融合蛋白并制备兔抗GST-MPS-1抗体。方法将MPS-1全长cDNA克隆至pGEX-5X载体内,转化大肠杆菌BL21,用IPTG诱导GST-MPS-1融合蛋白的表达。以分离纯化表达的蛋白免疫新西兰兔,制备抗GST-MPS-1抗体。用Western ... 目的在大肠杆菌中表达GST-MPS-1融合蛋白并制备兔抗GST-MPS-1抗体。方法将MPS-1全长cDNA克隆至pGEX-5X载体内,转化大肠杆菌BL21,用IPTG诱导GST-MPS-1融合蛋白的表达。以分离纯化表达的蛋白免疫新西兰兔,制备抗GST-MPS-1抗体。用Western blot和细胞免疫荧光染色法,检测兔抗GST-MPS-1抗体的特异性。结果经测序和酶切鉴定确认,MPS-1基因以正确的方式插入到载体中。此重组表达载体经IPTG诱导后,可高效表达相对分子质量(Mr)为36000的GST-MPS-1融合蛋白。以融合蛋白免疫兔获得的抗血清,经ELISA检测效价达到1×105。Western blot和细胞免疫荧光染色证实,该多克隆抗体能与MPS-1蛋白特异性结合。结论兔抗MPS-1特异性抗体的获得,为进一步检测MPS-1蛋白的表达和功能分析奠定了基础。 展开更多
关键词 胃癌 肿瘤相关抗原 mpS-1 融合蛋白 多克隆抗体
下载PDF
香蕉线条病毒MP功能域基因的克隆、原核表达及抗血清制备 被引量:2
13
作者 陈秀 饶雪琴 +1 位作者 阮小蕾 李华平 《华南农业大学学报》 CAS CSCD 北大核心 2014年第2期47-52,共6页
[目的]制备香蕉线条病毒广东分离物( BSV-GD) MP功能域基因编码蛋白的多克隆抗血清,为BSV基因编码蛋白功能的研究提供条件.[方法]对BSV-GD ORF3基因氨基酸序列进行生物信息学分析,得出MP功能域基因序列,克隆该基因并插入载体pET-... [目的]制备香蕉线条病毒广东分离物( BSV-GD) MP功能域基因编码蛋白的多克隆抗血清,为BSV基因编码蛋白功能的研究提供条件.[方法]对BSV-GD ORF3基因氨基酸序列进行生物信息学分析,得出MP功能域基因序列,克隆该基因并插入载体pET-28b(+)构建原核表达载体,经IPTG诱导表达后,采用超声波裂解法进行蛋白可溶性分析,利用组氨酸标签纯化试剂盒对目的融合蛋白进行纯化和回收,然后以纯化的目的蛋白为抗原免疫健康家兔制备其多克隆抗血清;通过Western-blot分析该抗血清的特异性,间接ELISA法检测该抗血清的效价.[结果和结论]MP功能域基因在ORF3中的序列为61~311 aa处,核酸序列长753 bp.试验克隆了该基因并成功构建了其原核表达载体pET28b-MP,经IPTG诱导1 h后表达了相对分子质量约为30800的融合蛋白6His&#183; MP.可溶性分析表明该融合蛋白以包涵体形式存在,纯化获得了高纯度的目的融合蛋白,以其为抗原成功制备了BSV-GD MP功能域基因编码蛋白的多克隆抗血清;分析表明该抗血清具有很强的特异性,其效价高达204800倍以上. 展开更多
关键词 香蕉线条病毒 mp功能域基因 原核表达 抗血清
下载PDF
酵母双杂交系统筛选Mps1相互作用蛋白 被引量:1
14
作者 施琼 翁亚光 《重庆医科大学学报》 CAS CSCD 2006年第6期854-856,888,共4页
目的:对前期筛出的与Mps1可能有相互作用的两种蛋白用酵母双杂交回转,验证与Mps1有相互作用的蛋白,为染色体分离机制研究提供线索,并为蛋白质相互作用网络提供资料。方法:应用酵母双杂交技术,将前期以pDBLeu-Mps1为诱饵质粒筛选成人肝脏... 目的:对前期筛出的与Mps1可能有相互作用的两种蛋白用酵母双杂交回转,验证与Mps1有相互作用的蛋白,为染色体分离机制研究提供线索,并为蛋白质相互作用网络提供资料。方法:应用酵母双杂交技术,将前期以pDBLeu-Mps1为诱饵质粒筛选成人肝脏cDNA文库,得到的Leu+LacZ+阳性克隆进一步回转酵母进行验证,即将文库质粒与诱饵质粒一对一重新转入酵母体内对相互作用进行验证。并对验证后的阳性克隆的外源片段进行测序及同源性分析。结果:用同源性分析,从人胚胎cDNA文库筛选到的与M ps1有相互作用的两个蛋白在酵母双杂交回转实验中仅有一个为阳性,另一个相互作用为阴性。结论:应用酵母双杂交系统从人胚胎cDNA文库中筛选并初步验证了与Mps1有相互作用的蛋白,为Mps1蛋白的功能研究奠定了基础。 展开更多
关键词 酵母双杂交技术 mps1 蛋白质相互作用
下载PDF
BMP-2在减阻牵张快速牙移动不同加力方式下的表达及对牙移动的影响 被引量:2
15
作者 彭早霞 李宁 +4 位作者 李佩 李美静 杨乐乐 陈曦 米丛波 《实用口腔医学杂志》 CAS CSCD 北大核心 2016年第1期53-57,共5页
目的:观察不同牵张力下减阻牵张快速牙移动中BMP-2的表达。方法:Beagle犬12只,随机均分为加力5、15 d、加力15 d保持固定10、90 d 4组。以■为移动牙每个组3只犬的6颗移动牙随机采用减阻—牵张方法、减阻-常规方法和常规方法各2颗。各... 目的:观察不同牵张力下减阻牵张快速牙移动中BMP-2的表达。方法:Beagle犬12只,随机均分为加力5、15 d、加力15 d保持固定10、90 d 4组。以■为移动牙每个组3只犬的6颗移动牙随机采用减阻—牵张方法、减阻-常规方法和常规方法各2颗。各组犬按预定时间处死并获取移动牙牙周组织块,免疫组化法染色并观察BMP-2表达。结果:各加力方式下BMP-2阳性表达分布区域相似,均在加力结束时达峰值,其中减阻牵张组峰值最大(P<0.05);牙移动距离最大(P<0.01)。加力各时间点,减阻常规组BMP-2阳性表达均强于常规方法组但不及减阻牵张组显著;保持固定90 d,3组无差异(P>0.05)。结论:减阻措施配合持续强牵张力可显著提高移动牙牵张新骨区BMP-2阳性表达加速牙周组织新骨形成。 展开更多
关键词 减阻牵张 快速牙齿移动 Bmp-2 骨改建
下载PDF
基于气机升降理论观察旋复代赭汤及其拆方对RE模型大鼠AMPK的影响 被引量:9
16
作者 田晶晶 杨幼新 +2 位作者 袁红霞 马艳 许云姣 《新中医》 CAS 2016年第3期230-234,共5页
目的:观察旋复代赭汤及其拆方对反流性食管炎(RE)模型大鼠腺苷酸活化蛋白激酶(AMPK)含量的影响。方法:将84只雄性Wistar大鼠,随机分成7组,即正常对照组、模型对照组、旋复代赭汤全方组及拆方各组(包括苦降组、甘升组、升降相因组)、西药... 目的:观察旋复代赭汤及其拆方对反流性食管炎(RE)模型大鼠腺苷酸活化蛋白激酶(AMPK)含量的影响。方法:将84只雄性Wistar大鼠,随机分成7组,即正常对照组、模型对照组、旋复代赭汤全方组及拆方各组(包括苦降组、甘升组、升降相因组)、西药组(兰索拉唑+莫沙必利),每组12只大鼠。对造模组大鼠采用"4.2 mm幽门夹+胃底2/3结扎术"制备反流性食管炎动物模型。从造模术后7天开始,正常对照组、模型对照组给予生理盐水灌胃,旋复代赭汤全方组及拆方各组分别给予相应药液,西药组给予(兰索拉唑+莫沙必利)灌胃,干预14天后,处死全部大鼠后,其中每组分别送2份大鼠食管组织制作线粒体超微结构切片,应用电镜观察食管下段黏膜组织线粒体超微结构变化,其余食管组织匀浆后应用ELISA测定AMPK含量的表达情况。结果:食管组织线粒体超微结构:模型对照组大鼠电镜下食管黏膜线粒体稀少,形态、大小不一,内膜、外膜及嵴断续模糊,部分线粒体呈肿胀、空泡化,偶见巨线粒体;旋复代赭汤全方组、西药组较模型对照组改善(P<0.05),甘升组、升降相因组较模型对照组也明显改善(P<0.05);各组AMPK含量:旋复代赭汤全方组、西药组较模型对照组降低(P<0.05);甘升组、升降相因组较模型组也降低(P<0.05)。结论:旋复代赭汤及其药物通过调节脾胃气机,降低食管组织AMPK含量,促进线粒体能量三磷酸腺苷(ATP)的生成代谢,增加线粒体数量,减少线粒体结构及食管黏膜损伤,调节线粒体能量代谢,治疗RE。 展开更多
关键词 反流性食管炎(RE) 旋复代赭汤 拆方 气机升降 能量代谢 腺苷酸活化蛋白激酶(AmpK)
下载PDF
ATOX1通过JAK2/STAT3通路促进肝癌细胞生物学行为的机制探讨
17
作者 马佳佳 张亚苹 +5 位作者 杨斌 赵美琪 蒋璐 黄小玉 范路畅 王凤梅 《天津医药》 CAS 2024年第9期907-912,共6页
目的探究肝细胞癌(HCC)中抗氧化剂1铜伴侣蛋白(ATOX1)表达的临床意义及其与肿瘤细胞增殖、迁移和侵袭的关系。方法分析人类基因组图谱数据库HCC癌组织和正常肝组织ATOX1 mRNA的表达。采用免疫组织化学染色检测15例HCC癌和癌旁组织中ATOX... 目的探究肝细胞癌(HCC)中抗氧化剂1铜伴侣蛋白(ATOX1)表达的临床意义及其与肿瘤细胞增殖、迁移和侵袭的关系。方法分析人类基因组图谱数据库HCC癌组织和正常肝组织ATOX1 mRNA的表达。采用免疫组织化学染色检测15例HCC癌和癌旁组织中ATOX1的表达。人肝癌细胞株Hep3B和HepG2分为对照组(NC组)、ATOX1敲低1组(si-ATOX1#1组)和ATOX1敲低2组(si-ATOX1#2组)。通过CCK-8细胞增殖实验、划痕实验、Transwell侵袭实验观察敲低ATOX1对癌细胞恶性生物学行为的影响。构建裸鼠异种皮下移植瘤模型,分析敲低ATOX1表达对移植瘤质量和体积的影响。Western blot检测移植瘤中Janus激酶2/信号转导和转录激活因子3(JAK2/STAT3)通路蛋白表达水平。结果生物信息学分析显示HCC癌组织中ATOX1 mRNA表达高于癌旁组织(P<0.05)。免疫组化染色发现HCC癌组织中ATOX1蛋白阳性率高于癌旁组织(93.33%vs.13.33%,P<0.01)。体外实验结果显示,siRNA敲低Hep3B、HepG2细胞中ATOX1蛋白表达后,癌细胞的增殖、迁移及侵袭能力均显著降低(均P<0.05)。小鼠体内实验中,sh-ATOX1敲低组裸鼠皮下移植瘤的体积和质量均显著小于sh-con组,JAK2/STAT3通路相关蛋白p-JAK2、p-STAT3、细胞周期蛋白D1(CyclinD1)及基质金属蛋白酶2表达均下降。结论ATOX1能够通过JAK2/STAT3通路促进HCC的增殖、迁移和侵袭,可能成为潜在的肿瘤标志物和治疗靶点。 展开更多
关键词 肝细胞 细胞运动 生物标记 肿瘤 抗氧化剂1铜伴侣蛋白 JAK2/STAT3通路
下载PDF
A viral movement protein targets host catalases for 26S proteasome-mediated degradation to facilitate viral infection and aphid transmission in wheat
18
作者 Shuyuan Tian Qingting Song +5 位作者 Wenmei Zhou Jingke Wang Yanbin Wang Wei An Yunfeng Wu Lei Zhao 《Molecular Plant》 SCIE CSCD 2024年第4期614-630,共17页
The infection of host plants by many different viruses causes reactive oxygen species(Ros)accumulation and yellowing symptoms,but the mechanisms through which plant viruses counteract RoS-mediated immunity to facilita... The infection of host plants by many different viruses causes reactive oxygen species(Ros)accumulation and yellowing symptoms,but the mechanisms through which plant viruses counteract RoS-mediated immunity to facilitate infection and symptom development have not been fully elucidated.Most plant viruses are transmitted by insect vectors in the field,but the molecular mechanisms underlying virus-host-insect interactions are unclear.In this study,we investigated the interactions among wheat,barley yellow dwarf virus(BYDV),and its aphid vector and found that the BYDV movement protein(MP)interacts with both wheat catalases(CATs)and the 26S proteasomeubiquitin receptor non-ATPase regulatorysubunit2homolog(PSMD2)to facilitate the 26S proteasome-mediateddegradation of CATs,promotingviral infection,disease symptom development,and aphid transmission.Overexpression of the BYDV MP gene in wheat enhanced the degradation of CATs,which leading to increased accumulation of ROS and thereby enhanced viral infection.Interestingly,transgenic wheat lines overexpressing BYDV MP showed significantly reduced proliferation of wingless aphids and an increased number of winged aphids.Consistent with this observation,silencing of CAT genes also enhanced viral accumulation and reduced the proliferation of wingless aphids but increased the occurrence of winged aphids.In contrast,transgenic wheat plants overexpressing TaCAT1 exhibited the opposite changes and showed increases in grain size and weight upon infection with BYDV.Biochemical assays demonstrated that BYDV MP interacts with PSMD2 and promotes 26S proteasome-mediated degradation of TaCAT1 likely in a ubiquitination-independent manner.Collectively,our study reveals a molecular mechanism by which a plant virus manipulates the Ros production system of host plants to facilitate viral infection and transmission,shedding new light on the sophisticated interactions among viruses,host plants,and insect vectors. 展开更多
关键词 babarley yellow dwarf virus movement protein reactive oxygen species APHID CATALASE 26S proteasomeubiquitin receptor PSMD2
原文传递
盐酸双吗啡肽对耐药食管癌细胞迁移和侵袭能力的影响
19
作者 石晓丽 苏治国 +4 位作者 张英 杨国帅 彭温暖 王晨 马霖 《精准医学杂志》 2024年第6期547-551,共5页
目的探讨骨形态发生蛋白(BMP)小分子抑制剂盐酸双吗啡肽(Dorsomorphin)对耐药食管癌EC109/PTX细胞迁移和侵袭能力的影响。方法MTT实验检测不同浓度盐酸双吗啡肽对EC109/PTX细胞增殖的影响并确定后续实验的浓度。以筛选出的1μmol/L盐酸... 目的探讨骨形态发生蛋白(BMP)小分子抑制剂盐酸双吗啡肽(Dorsomorphin)对耐药食管癌EC109/PTX细胞迁移和侵袭能力的影响。方法MTT实验检测不同浓度盐酸双吗啡肽对EC109/PTX细胞增殖的影响并确定后续实验的浓度。以筛选出的1μmol/L盐酸双吗啡肽的浓度作为试验组,同时设立空白对照组,处理EC109/PTX细胞48 h。采用流式细胞仪检测盐酸双吗啡肽对EC109/PTX细胞凋亡的影响。采用划痕实验和Transwell实验检测两组EC109/PTX细胞的迁移和侵袭能力。采用Western blotting实验检测两组EC109/PTX细胞中Vimentin、E-cadherin、N-cadherin蛋白相对表达水平。结果1μmol/L盐酸双吗啡肽对EC109/PTX细胞的细胞抑制率为(9.89±1.12)%。培养第48小时时,对照组和实验组EC109/PTX细胞的凋亡率比较差异无显著性(P>0.05)。划痕实验和Transwell实验显示,与对照组相比,实验组EC109/PTX细胞的迁移率和细胞穿膜率显著降低(t=85.42、19.65,P<0.05)。Western blotting实验显示,与对照组相比,[JP2]实验组细胞中Vimentin、N-cadherin[JP]蛋白相对表达量显著降低(t=19.40、41.79,P<0.05),N-cadherin蛋白表达量显著增高(t=58.12,P<0.05)。结论BMP抑制剂盐酸双吗啡肽能影响耐药食管癌细胞中EMT相关蛋白表达,从而抑制耐药肿瘤细胞的迁移和侵袭。 展开更多
关键词 骨形态发生蛋白质4 Dorsomorphin 抗药性 肿瘤 食管肿瘤 细胞运动 肿瘤浸润 上皮-间质转化 基因表达调控 肿瘤
下载PDF
孕鼠正畸牙移动牙周组织DMP1表达变化的研究 被引量:1
20
作者 莫水学 陈扬熙 《广西医科大学学报》 CAS 2010年第6期826-828,共3页
目的:观察孕鼠牙周组织牙本质基质蛋白1(DMP1)的表达,以及在怀孕及非孕状态下正畸施力后DMP1的表达变化,探讨其在正畸牙周改建中的可能作用。方法:大鼠戴入牙移动装置,加力使其上颌第一磨牙朝近中移动;免疫组织化学法检测牙周组织DMP1... 目的:观察孕鼠牙周组织牙本质基质蛋白1(DMP1)的表达,以及在怀孕及非孕状态下正畸施力后DMP1的表达变化,探讨其在正畸牙周改建中的可能作用。方法:大鼠戴入牙移动装置,加力使其上颌第一磨牙朝近中移动;免疫组织化学法检测牙周组织DMP1的表达变化。结果:大鼠牙齿及牙周组织的多种细胞有DMP1的表达;孕鼠牙周组织DMP1的表达强于非孕鼠,且具有孕中期表达最强、早期稍低而晚期最低的特点;大鼠张力侧牙周组织的表达强于压力侧;加力后,孕鼠压力侧牙周组织中DMP1的表达下降,而张力侧的表达增强。结论:孕鼠牙周组织DMP1的表达可能受到孕酮的上调作用;孕期正畸牙周组织改建过程中,DMP1可能主要起到促进矿化和骨形成的作用。 展开更多
关键词 正畸牙移动 孕鼠 孕酮 牙本质基质蛋白1
下载PDF
上一页 1 2 16 下一页 到第
使用帮助 返回顶部