目的探讨长期使用核苷类似物(NAs)是否导致小鼠肝脏线粒体DNA(mt DNA)ND1和ND4区损伤。方法 7周龄雌性Balb/c小鼠25只,采用简单随机分组法分为5组,对照组和4种核苷类似物组,每组各5只。实验组司他夫定(D4T)50 mg/kg,齐多夫定(AZT)100 mg...目的探讨长期使用核苷类似物(NAs)是否导致小鼠肝脏线粒体DNA(mt DNA)ND1和ND4区损伤。方法 7周龄雌性Balb/c小鼠25只,采用简单随机分组法分为5组,对照组和4种核苷类似物组,每组各5只。实验组司他夫定(D4T)50 mg/kg,齐多夫定(AZT)100 mg/kg,拉米夫定(3TC)50 mg/kg和去羟肌苷(DDI)50 mg/kg,对照组为双蒸水,分别腹腔内注射,每周5次,连续3个月。留取各组肝组织,应用激光捕获显微技术获取肝细胞,对mt DNA ND1和ND4区克隆和测序。结果其余各组肝细胞的mt DNA ND4序列距离与参考序列的平均距离与对照组比较,差异均有高度统计学意义(P<0.01);AZT组肝细胞的mt DNA ND4平均d N与对照组比较,差异有统计学意义(P<0.05);肝细胞mt DNA ND1的序列距离与参考序列的平均距离,AZT组和3TC组序列距离与对照组比较,差异均有统计学意义(P<0.01);DDI组肝组织mt DNA ND1的平均d S与对照组比较,差异有统计学意义(P<0.05);AZT组和3TC组肝细胞mt DNA ND1的平均d S与对照组比较,差异均有统计学意义(P<0.05)。结论长期暴露于NAs可导致小鼠肝细胞mt DNA ND1和ND4区病变。展开更多
Acipenseriformes is an endangered primitive fish group, which occupies a special place in the history of ideas concerning fish evolution, even in vertebrate evolution. However, the classification and evolution of the ...Acipenseriformes is an endangered primitive fish group, which occupies a special place in the history of ideas concerning fish evolution, even in vertebrate evolution. However, the classification and evolution of the fishes have been debated. The mitochondrial DMA (mtDNA) ND4L and partial A7D4 genes were first sequenced in twelve species of the order Acipenseriformes, including endemic Chinese species. The following points were drawn from DNA sequences analysis: (i) the two species of Huso can be ascribed to Acipenser; (ii) A. dabryanus is the mostly closely related to A. sinensis, and most likely the landlocked form of A. sinensis; (iii) genus Acipenser in trans-Pacific region might have a common origin; (iv) mtDNA ND4L and ND4 genes are the ideal genetic markers for phylogenetic analysis of the order Acipenseriformes.展开更多
文摘目的探讨长期使用核苷类似物(NAs)是否导致小鼠肝脏线粒体DNA(mt DNA)ND1和ND4区损伤。方法 7周龄雌性Balb/c小鼠25只,采用简单随机分组法分为5组,对照组和4种核苷类似物组,每组各5只。实验组司他夫定(D4T)50 mg/kg,齐多夫定(AZT)100 mg/kg,拉米夫定(3TC)50 mg/kg和去羟肌苷(DDI)50 mg/kg,对照组为双蒸水,分别腹腔内注射,每周5次,连续3个月。留取各组肝组织,应用激光捕获显微技术获取肝细胞,对mt DNA ND1和ND4区克隆和测序。结果其余各组肝细胞的mt DNA ND4序列距离与参考序列的平均距离与对照组比较,差异均有高度统计学意义(P<0.01);AZT组肝细胞的mt DNA ND4平均d N与对照组比较,差异有统计学意义(P<0.05);肝细胞mt DNA ND1的序列距离与参考序列的平均距离,AZT组和3TC组序列距离与对照组比较,差异均有统计学意义(P<0.01);DDI组肝组织mt DNA ND1的平均d S与对照组比较,差异有统计学意义(P<0.05);AZT组和3TC组肝细胞mt DNA ND1的平均d S与对照组比较,差异均有统计学意义(P<0.05)。结论长期暴露于NAs可导致小鼠肝细胞mt DNA ND1和ND4区病变。
文摘Acipenseriformes is an endangered primitive fish group, which occupies a special place in the history of ideas concerning fish evolution, even in vertebrate evolution. However, the classification and evolution of the fishes have been debated. The mitochondrial DMA (mtDNA) ND4L and partial A7D4 genes were first sequenced in twelve species of the order Acipenseriformes, including endemic Chinese species. The following points were drawn from DNA sequences analysis: (i) the two species of Huso can be ascribed to Acipenser; (ii) A. dabryanus is the mostly closely related to A. sinensis, and most likely the landlocked form of A. sinensis; (iii) genus Acipenser in trans-Pacific region might have a common origin; (iv) mtDNA ND4L and ND4 genes are the ideal genetic markers for phylogenetic analysis of the order Acipenseriformes.
