DETECTION OF SBEM-MRNA IN PERIPHERAL BLOOD OF PATIENTS WITH BREAST CANCER AND ITS CLINICAL SIGNIFICANCE

Objective： The aim of the present study is to explore the expression of a specific marker of breast cancer, small breast epithefial mucin （SBEM） mRNA, in peripheral blood and to investigate its significance. Methods： The expressions of SBEM-mRNA in peripheral blood of 67 patients with breast cancer, 16 patients with benign breast disease, and 20 normal healthy volunteers were detected with nested reverse transcription-polymerase chain reaction （Nested-RT-PCR）. Results： SBEMomRNA was negative in healthy individuals and patients with benign breast tumor, but 50.7%（34/67） of breast cancer patients showed positive expression of SBEM-mRNA in peripheral blood, of whom 25%（2/8） were in stage Ⅰ, 45.8%（11/24） in stage Ⅱ, 43.75%（11/24） in stage Ⅲ and 73.7（14/19） in stage Ⅳ. The positive rate in stage Ⅳ was higher than that in stage Ⅰ, Ⅱ, Ⅲ （P〈0.05）. Expressions of SBEM-mRNA had no correlation with age, carcinoma size, pathological type, ER and PR state （P〉0.05）. Concision： SBEM-mRNA is specifically expressed in breast cancer and it may act as a marker for the detection of of breast cancer.

ZnO纳米粒子通过线粒体通路抑制小鼠光感受器细胞Na^＋/K^＋-ATP酶表达和活性的作用研究

氧化锌(ZnO)纳米粒子已被发现具有生物毒性,氧化应激被认为是最重要的因素之一。前期实验证实,ZnO纳米粒子能显著减少锰超氧化物歧化酶(Mn SOD)蛋白的表达,降低Mn SOD活性。本文通过检测乳酸脱氢酶(LDH)释放、线粒体活性氧(ROS)水平和膜电位(Δφm)、延迟整流钾电流变化和Na^+/K^+-ATP酶的表达及活性等变化,检测ZnO纳米粒子对小鼠光感受器细胞的细胞毒作用。结果表明,ZnO纳米粒子可显著增强小鼠光感受器细胞中LDH的释放、增加线粒体内ROS水平并下调Δφm、阻断延迟整流钾电流,同时降低Na^+/K^+-ATP酶的表达及活性,从而对小鼠视网膜光感受器细胞产生细胞毒作用,提示ZnO纳米粒子可通过线粒体通路引起氧化应激,从而抑制小鼠光感受器细胞Na^+/K^+-ATP酶表达和活性,产生细胞毒性,导致细胞死亡。本文的研究结果有助于理解ZnO纳米粒子引起细胞毒性的作用机理。