Mugwort has many traditional uses around the world. The Chumash Indians of California use it to treat imbalances that women may suffer such as premenstrual syndrome, dysmenorrhea and menopausal symptoms. The plant con...Mugwort has many traditional uses around the world. The Chumash Indians of California use it to treat imbalances that women may suffer such as premenstrual syndrome, dysmenorrhea and menopausal symptoms. The plant contains a sesquiterpene that appears to work through a serotonergic mechanism and may be beneficial for women. Mugwort therapy is safer for menopausal women than hormone replacement therapy. Children affected by attention deficit hyperactivity disorder benefit from mugwort therapy. There is no doubt that mugwort therapy is safer for these children than methylphenidate or amphetamine.展开更多
Background Excessive expression of thymic stromal lymphopoietin (TSLP) has been demonstrated in asthmatic airway epithelia and in nasal epithelia from animal models of allergic rhinitis (AR), but the evidence of e...Background Excessive expression of thymic stromal lymphopoietin (TSLP) has been demonstrated in asthmatic airway epithelia and in nasal epithelia from animal models of allergic rhinitis (AR), but the evidence of expression of TSLP in nasal epithelial cells (NECs) of patients with AR is lacking. We aimed to investigate the expression of TSLP in NECs of patients with mugwort sensitive-seasonal AR and determine whether it is associated with severity of symptoms and the number of infiltrated eosinophils in nasal mucosa. Methods NECs specimens were obtained by scraping with plastic curettes from the nasal inferior turbinates of patients with mugwort pollen sensitive-seasonal AR (n=22) and nonallergic controls (n=11) during last peak mugwort pollen season. The severity of nasal symptom was assessed using a Visual Analog Scale (VAS). In addition, serum mugwort pollen IgE levels were tested from each patient. In situ hybridization (ISH) was performed to test the messenger RNA (mRNA) of TSLP in the NECs. Furthermore, immunohistochemical staining (IHC) was scored to evaluate the expression of TSLP and eosinophil cell count was made by May-Grunwald/Giemsa staining. The correlation between expression of TSLP and all other parameters was analyzed in this study. Results The mRNA level of TSLP was significantly increased in NECs of patients with AR compared with the nonallergic control group (P 〈0.05). In addition, IHC results showed that expression of TSLP in NECs from patients with AR was up-regulated which was correlated with VAS score (r=0.598; P 〈0.05) and nasal eosinophils count (r=0.702; P 〈0.05), but it was unrelated with mugwort pollen specific IgE level. Conclusions These preliminary findings indicate a potential relationship between TSLP expression, severity of symptoms and nasal eosinophils count in pathogenesis of AR, but TSLP expression did not correlate with mugwort pollen specific IgE level. The elevated expression of TSLP might play a critical role in local atopical responses of AR. In the future, the TSLP has the potential to be one of the most important molecular markers for AR diagnoses and assessment.展开更多
探索优化大孔树脂分离纯化艾绒总黄酮的工艺。本实验采用单因素实验优化分离纯化工艺。经过研究,发现HPD100大孔树脂具有最优的吸附和解析附能力。采用90 min的上样时间,pH=3,吸附温度40℃,上样液质量浓度0.2 mg/mL,上样液流速1.5 m L/m...探索优化大孔树脂分离纯化艾绒总黄酮的工艺。本实验采用单因素实验优化分离纯化工艺。经过研究,发现HPD100大孔树脂具有最优的吸附和解析附能力。采用90 min的上样时间,pH=3,吸附温度40℃,上样液质量浓度0.2 mg/mL,上样液流速1.5 m L/min,吸附率可达到68.5%。用体积分数80%乙醇洗脱,流速为1.5 m L/min,洗脱液用量为100 m L,洗脱率高达到60.45%。在此吸附和解析条件下,艾绒总黄酮的纯度由5.9%提升到13.5%,提纯效果好。展开更多
White mugwort (Artemisia lactiflora Wall.) is known as a medicinal herb containing phytochemicals with antioxidant and bio-activity.The optimal concentration of ethanol was investigated for extracting polyphenols from...White mugwort (Artemisia lactiflora Wall.) is known as a medicinal herb containing phytochemicals with antioxidant and bio-activity.The optimal concentration of ethanol was investigated for extracting polyphenols from the fresh aerial part and dried powder of white mugwort as expressed by total phenolic content (TPC),phenolic compounds,antioxidant,anti-inflammatory,and anti-adipogenic activity.Among various ethanol concentrations (0,25,50 or 95%) used in the extraction process,the freeze-dried extract from fresh aerial part (FE) extracted with 95% ethanol expressed the highest antioxidant activity (DPPH radical scavenging IC50 of 0.817 ± 0.06 μl/mg dry weight (DW) and FRAP value of 11.40 ± 0.86 mmol Fe(II)/g DW) and the highest TPC (61.46 ± 3.09 mgGAE/g DW).While,the freeze-dried extract from dried powder (PE) exhibited the prominent antioxidant properties (DPPH radical scavenging IC50 of 0.975 ± 0.03 μl/mg DW and FRAP value of 07.60 ± 0.22 mmol Fe(II)/g DW),TPC (48.11 ± 1.45 mgGAE/g DW) when extracted with 50% ethanol.Seven phenolic compounds—two phenolic acids (gallic acid and tannic acid) and five flavonoids (apigenin,isoquercetin,quercetin,rutin,and catechin) were identified in FE and PE using HPLC-DAD/MS.The inflammatory genes were down-regulated in lipopolysaccharide-stimulated THP-1 monocytes treated with PE,suggesting anti-inflammatory activity.Lipid accumulation in 3T3-L1 adipocytes treated with FE at 60 μ gGAE/mL decreased to 12.8%,while the decrease of 36.8% was detected in PE.Hence,flavonoids were suggested to be responsible for the stronger bioactivity of PE over FE.Moreover,this work originally revealed the investigation of the anti-inflammatory and anti-adipogenic activity of white mugwort on THP-1 and 3T3-L1 cells.展开更多
文摘Mugwort has many traditional uses around the world. The Chumash Indians of California use it to treat imbalances that women may suffer such as premenstrual syndrome, dysmenorrhea and menopausal symptoms. The plant contains a sesquiterpene that appears to work through a serotonergic mechanism and may be beneficial for women. Mugwort therapy is safer for menopausal women than hormone replacement therapy. Children affected by attention deficit hyperactivity disorder benefit from mugwort therapy. There is no doubt that mugwort therapy is safer for these children than methylphenidate or amphetamine.
文摘Background Excessive expression of thymic stromal lymphopoietin (TSLP) has been demonstrated in asthmatic airway epithelia and in nasal epithelia from animal models of allergic rhinitis (AR), but the evidence of expression of TSLP in nasal epithelial cells (NECs) of patients with AR is lacking. We aimed to investigate the expression of TSLP in NECs of patients with mugwort sensitive-seasonal AR and determine whether it is associated with severity of symptoms and the number of infiltrated eosinophils in nasal mucosa. Methods NECs specimens were obtained by scraping with plastic curettes from the nasal inferior turbinates of patients with mugwort pollen sensitive-seasonal AR (n=22) and nonallergic controls (n=11) during last peak mugwort pollen season. The severity of nasal symptom was assessed using a Visual Analog Scale (VAS). In addition, serum mugwort pollen IgE levels were tested from each patient. In situ hybridization (ISH) was performed to test the messenger RNA (mRNA) of TSLP in the NECs. Furthermore, immunohistochemical staining (IHC) was scored to evaluate the expression of TSLP and eosinophil cell count was made by May-Grunwald/Giemsa staining. The correlation between expression of TSLP and all other parameters was analyzed in this study. Results The mRNA level of TSLP was significantly increased in NECs of patients with AR compared with the nonallergic control group (P 〈0.05). In addition, IHC results showed that expression of TSLP in NECs from patients with AR was up-regulated which was correlated with VAS score (r=0.598; P 〈0.05) and nasal eosinophils count (r=0.702; P 〈0.05), but it was unrelated with mugwort pollen specific IgE level. Conclusions These preliminary findings indicate a potential relationship between TSLP expression, severity of symptoms and nasal eosinophils count in pathogenesis of AR, but TSLP expression did not correlate with mugwort pollen specific IgE level. The elevated expression of TSLP might play a critical role in local atopical responses of AR. In the future, the TSLP has the potential to be one of the most important molecular markers for AR diagnoses and assessment.
文摘探索优化大孔树脂分离纯化艾绒总黄酮的工艺。本实验采用单因素实验优化分离纯化工艺。经过研究,发现HPD100大孔树脂具有最优的吸附和解析附能力。采用90 min的上样时间,pH=3,吸附温度40℃,上样液质量浓度0.2 mg/mL,上样液流速1.5 m L/min,吸附率可达到68.5%。用体积分数80%乙醇洗脱,流速为1.5 m L/min,洗脱液用量为100 m L,洗脱率高达到60.45%。在此吸附和解析条件下,艾绒总黄酮的纯度由5.9%提升到13.5%,提纯效果好。
基金funded by Thailand Researchers and Research for Industry Grants(RRi)(Grant No.PHD61I0001)。
文摘White mugwort (Artemisia lactiflora Wall.) is known as a medicinal herb containing phytochemicals with antioxidant and bio-activity.The optimal concentration of ethanol was investigated for extracting polyphenols from the fresh aerial part and dried powder of white mugwort as expressed by total phenolic content (TPC),phenolic compounds,antioxidant,anti-inflammatory,and anti-adipogenic activity.Among various ethanol concentrations (0,25,50 or 95%) used in the extraction process,the freeze-dried extract from fresh aerial part (FE) extracted with 95% ethanol expressed the highest antioxidant activity (DPPH radical scavenging IC50 of 0.817 ± 0.06 μl/mg dry weight (DW) and FRAP value of 11.40 ± 0.86 mmol Fe(II)/g DW) and the highest TPC (61.46 ± 3.09 mgGAE/g DW).While,the freeze-dried extract from dried powder (PE) exhibited the prominent antioxidant properties (DPPH radical scavenging IC50 of 0.975 ± 0.03 μl/mg DW and FRAP value of 07.60 ± 0.22 mmol Fe(II)/g DW),TPC (48.11 ± 1.45 mgGAE/g DW) when extracted with 50% ethanol.Seven phenolic compounds—two phenolic acids (gallic acid and tannic acid) and five flavonoids (apigenin,isoquercetin,quercetin,rutin,and catechin) were identified in FE and PE using HPLC-DAD/MS.The inflammatory genes were down-regulated in lipopolysaccharide-stimulated THP-1 monocytes treated with PE,suggesting anti-inflammatory activity.Lipid accumulation in 3T3-L1 adipocytes treated with FE at 60 μ gGAE/mL decreased to 12.8%,while the decrease of 36.8% was detected in PE.Hence,flavonoids were suggested to be responsible for the stronger bioactivity of PE over FE.Moreover,this work originally revealed the investigation of the anti-inflammatory and anti-adipogenic activity of white mugwort on THP-1 and 3T3-L1 cells.