This research investigates the mechanical and thermal properties of Morus alba combined with polylactic acid in comparison with other natural fibers. The study uses three different fiber and PLA compositions - 20%, 30...This research investigates the mechanical and thermal properties of Morus alba combined with polylactic acid in comparison with other natural fibers. The study uses three different fiber and PLA compositions - 20%, 30%, and 40% respectively - to produce composite materials. In addition, another composite with the same fiber volume is treated with a 4% NaOH solution to improve mechanical properties. The composites are processed by twin-screw extrusion, granulation, and injection molding. Tensile strength measurements of raw fibers and NaOH-treated fibers were carried out using a single-fiber tensile test with a gauge length of 40 mm. It was observed that the NaOH surface treatment increases the resistance against tensile loading and exhibited improved properties for raw fiber strands. The diameter of the fibers was measured using optical microscopy. During this research, flexural tests, impact tests, differential scanning calorimetry (DSC), and heat deflection temperature measurements (HDT) were conducted to evaluate the mechanical and thermal properties of the developed composite samples. The results indicate that the mechanical properties of NaOH-treated Morus alba-reinforced polylactic acid outperform both virgin PLA samples and untreated Morus alba samples.展开更多
This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·k...This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·kg-1 MAEs treatment significantly reduced the NDEA-induced hepatic nodules incidence and hepatocellular carcinoma incidence by 58.30% and 41.70% compared to the model group. Meanwhile, MAEs significantly restored the elevated the liver function enzymes, inhibited the tumor necrosis factor alpha and interleukin-6 levels, elevated the serum interleukin-10 and interferon-γ and increased hepatic glutathione-S-transferase and UDP-glucuronosyltransferase 2B1 enzyme activity. Moreover, 150 mg·kg-1 MAEs supplement enhanced glutathione content and the activities of superoxide dismutase, catalase, glutathione peroxidase activities but reduced the malondialdehyde and thiobarbituric acid-reactive substances content by 37.90% and 44.52%. Furthermore, MAEs pretreatment maintained nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1, heme oxygenase-1, and NAD(P)H: quinine oxidoreductase1 stimulation and inhibited the expression of TNF-α, nuclear factor-kappaB (NF-κB), and cyclooxygenase-2 (COX-2), indicating that MAEs exhibit effectively prevention effects against liver cancer via decreased lipid peroxidation, induced Nrf2-mediated antioxidant enzymes and attenuating the inflammatory mediators COX-2 through NF-κB pathway. Thus, MAEs of mulberry variety PR-01 may be used as a good functional dietary supplement against liver cancer.展开更多
为揭示叶果间的内在作用规律,以桑树(Morus alba L.)当年生果枝为研究对象,分析不同大小果枝上叶片特征和果实性状的差异及其二者间的相互关系。结果表明:随着果枝由小到大,果枝上的叶片数与总叶干重显著增加,而叶形指数却无显著变化;...为揭示叶果间的内在作用规律,以桑树(Morus alba L.)当年生果枝为研究对象,分析不同大小果枝上叶片特征和果实性状的差异及其二者间的相互关系。结果表明:随着果枝由小到大,果枝上的叶片数与总叶干重显著增加,而叶形指数却无显著变化;桑葚数与总桑葚干重也有显著增加,桑葚含水量无显著变化。桑葚数与叶片数、总叶干重、总叶面积及其平均桑葚干重显著正相关。研究结果证实了果实的性状与叶片特征之间存在极密切关系。当年生果枝上的桑葚数量多少取决于果枝上的叶片总数,平均桑葚的干重则与果枝上的平均叶面积显著相关。展开更多
This paper aimed to explore the correlation between Morus alba L. leaf node and its size or mass change and provide a theoretical basis for the development of scientific Morus alba L. cultivation technology. During 20...This paper aimed to explore the correlation between Morus alba L. leaf node and its size or mass change and provide a theoretical basis for the development of scientific Morus alba L. cultivation technology. During 2016-2017,the correlation between Morus alba L. leaf node and its size or mass change was investigated and researched. Results showed that through the correlation coefficient analysis,there was a weakly positive correlation between the leaf node and some indicators such as( leaf length ÷ leaf width) and petiole length,there was a weakly negative correlation between the leaf node and some indicators such as leaf width,( leaf length + leaf width),( leaf length × leaf width) and leaf mass,and there was a weakly negative correlation between the leaf node and the other indicators; there was a highly positive correlation between the leaf mass and some indicators such as Morus alba L. leaf length,leaf width,( leaf length + leaf width) and( leaf length × leaf width),there was a significantly positive correlation between the leaf mass and some indicators such as petiole transverse diameter in width and petiole mass,and there was a weakly positive correlation between the leaf mass and some indicators such as petiole transverse diameter in thickness. In terms of significant level of difference,it was found that there was no significant difference in the correlation coefficients between the leaf nodes on the Morus alba L. shoots and some indicators such as leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width) and leaf mass; there was an extremely significant difference in the correlation coefficients between the leaf mass and some indicators such as Morus alba L. leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width),petiole transverse diameter in width and petiole mass; there was no significant difference in the correlation coefficients between the leaf mass and the other indicators. The regression analysis was performed on the leaf node and leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width),( leaf length ÷ leaf width),petiole length,petiole diameter in width,petiole diameter in thickness,petiole mass and leaf mass. Significance F values were0. 4864,0. 1995,0. 2888,0. 3004,0. 2350,0. 2407,0. 8925,0. 5227,0. 7022 and 0. 2000,respectively,that is,there was an extremely significant difference between Morus alba L. leaf node and petiole diameter in width,there was a significant difference between Morus alba L.leaf node and petiole mass,and there was no significant difference between Morus alba L. leaf node and the other indicators. The comparative analysis of quadratic curve regression equation and linear regression equation was performed on some indicators such as leaf length,and it was found that the R value between the leaf node and petiole diameter in width was in line with the logarithmic curve regression equation,and the other R values of quadratic curve regression equation was larger than the other R values of linear regression equation,indicating that different Morus alba L. leaf nodes,leaf length,leaf mass and other indicators were in line with the quadratic curve regression equation. It was concluded that by investigating the leaf node on the Morus alba L. shoots and regression equation,we could predict the leaf size,leaf mass and other indicators,and we could take the leaf node on the Morus alba L. shoots as a main factor affecting the Morus alba L. leaf size and mass.展开更多
White mulberry tree (Morus alba L) is cultivated throughout Asia and Europe, including Poland. The leaves and root bark preparations from Morus alba have been used in traditional phytomedicine. The objective of the pr...White mulberry tree (Morus alba L) is cultivated throughout Asia and Europe, including Poland. The leaves and root bark preparations from Morus alba have been used in traditional phytomedicine. The objective of the present study was to compare chemical composition and antioxidative activity of aqueous extracts prepared from Polish variety of Morus alba leaves at the laboratory (L) and pilot plant scale (PP) conditions. Proximate composition, phenolic acids profile (HPLC/MS), flavonol glicosides (HPLC/ MS), polyphenols (Folin-Ciocalteu assay), and the antioxidant activity (ABTS and DPPH assay) of the extracts were determined. The main phenolic compounds were identified as gallic, protocatechuic, phydroxybenzoic, vanillic, chlorogenic, caffeic, p-coumaric, ferulic, and sinapic acids. Chlorogenic acid was the main phenolic constituent of both extracts. The flavonols fraction contained rutin, quercetin 3-β-D-glucoside, and kaempferol 3-β-D- glucopyranoside. Total concentration of phenolic compounds were 7.9 g and 14.4 g gallic acid equivalent/100 g extract, and antioxidant activity was 137.1 and 214.1 μMol Trolox equivalent/g dry weight for the PP and L extracts, respectively. We concluded that current pilot plant process is less efficient than laboratory process at the aqueous extraction of bioactive components from Morus alba dried leaves. Potential improvements may include increasing efficacy of the extraction, decreasing losses of bioactive components during the process, or both.展开更多
Ten quantitative morphological characters were studied in 56 Morus alba L. trees representing three natural populations from the trans-Himalayan Ladakh region. The altitude of collection sites ranged from 2815 to 3177...Ten quantitative morphological characters were studied in 56 Morus alba L. trees representing three natural populations from the trans-Himalayan Ladakh region. The altitude of collection sites ranged from 2815 to 3177 m above the sea level(asl). Coefficient of variation(CV) showed high phenotypic variation in M. alba. Linear regression analysis revealed that leaf and fruit size decreases with an increase in altitude. High CV was observed for leaf length, leaf width, petiole length, leaf area, internodal distance, number of nodes, bud length, fruit length, fruit width and fruit weight. Similarly, a high phenotypic plasticity index was observed for bud length, leaf length, leaf width, petiole length, leaf area, inter-nodal distance, number of nodes, fruit length, fruit width and fruit weight. For every 100 m increase in altitude, leaf length, leaf width and leaf area decreased by 1 cm, 0.8 cm and 16.6 cm2, respectively. Analysis of covariance showed a predominant altitudinal effect on the morphological characters in comparison to the population effect. A small change in the altitude caused significant change in the plant morphological characteristics. The present investigation represents to our knowledge the first study addressing phenotypic variation in mulberryalong an altitudinal gradient.展开更多
Morus alba(white mulberry) mesophyll protoplasts were isolated from leaves of 30-45 day old sterile shoots,with protoplast yields of 2.5 x 107 g-1/F.W. after purification. The protoplasts were cultured in a modified K...Morus alba(white mulberry) mesophyll protoplasts were isolated from leaves of 30-45 day old sterile shoots,with protoplast yields of 2.5 x 107 g-1/F.W. after purification. The protoplasts were cultured in a modified K8P liquid medium containing 0.2 mg/L 2,4-D(2,4- Dichlorophe-noxy acetic acid), 1 mg/L NAA(Naphthyl acetic acid) and 0.5 mg/L BA(6-benzylaminopurine). A low plating density (5 x 104/ml) proved to be favourable to the division of protoplast-derived cells. The first divisioll occurred 4 days after culture, and the division frequency reached 24% at 10 days. A number of cell colonies and microcalli formed in 6 weeks. The microcalli were transferred onto MSB medium with 0.5 mg/L NAA and 0.5 mg/L BA for further proliferation. Shoot formation was initiated when the calli of 3-4 mm in size were transferred onto MSB differentiation medium with 0.1 mg/L NAA and 1 mg/L BA. The frequency of shoot formation was 35%. The shoots of 4-5 cm in height were excised from the callus and rooted on half strength MS medium with 0.5 mg/L IBA and 0.1 mg/L BA. After transplantation into pots, the regenerated plants grew vigorously in the phytotron.展开更多
The aim of the study was to develop the technology of muesli via analyzing the addition of white mulberry fruit. The first step involved the selection of ingredients from bioactive compounds. Next, the technology of p...The aim of the study was to develop the technology of muesli via analyzing the addition of white mulberry fruit. The first step involved the selection of ingredients from bioactive compounds. Next, the technology of production was developed. Then, the antioxidant activity of new muesli with potentially pro-health model systems was estimated. Muesli was characterized in terms of its basic nutritional value, and antioxidant potential. The ability to inactivate DPPH and ABTS radicals was estimated. In addition, the amount of fat in muesli was calculated. As part of this calculation, fatty acid composition and content of primary and secondary oxidation products were ascertained. The last stage of the project included sensory analysis of the resultant products. Our studies showed that the new product-muesli had high nutritional value, high antioxidant potential and a positive fatty acid composition. And sensory analysis showed that the product was attractive.展开更多
The present review is aimed at providing a comprehensive summary on the botany,utility,phytochemistry,pharmacology,and clinical trials of Morus alba(mulberry or sang shu).The mulberry foliage has remained the primary ...The present review is aimed at providing a comprehensive summary on the botany,utility,phytochemistry,pharmacology,and clinical trials of Morus alba(mulberry or sang shu).The mulberry foliage has remained the primary food for silkworms for centuries.Its leaves have also been used as animal feed for livestock and its fruits have been made into a variety of food products.With flavonoids as major constituents,mulberry leaves possess various biological activities,including antioxidant,antimicrobial,skin-whitening,cytotoxic,anti-diabetic,glucosidase inhibition,anti-hyperlipidemic,anti-atherosclerotic,anti-obesity,cardioprotective,and cognitive enhancement activities.Rich in anthocyanins and alkaloids,mulberry fruits have pharmacological properties,such as antioxidant,anti-diabetic,anti-atherosclerotic,anti-obesity,and hepatoprotective activities.The root bark of mulberry,containing flavonoids,alkaloids and stilbenoids,has antimicrobial,skin-whitening,cytotoxic,anti-inflammatory,and anti-hyperlipidemic properties.Other pharmacological properties of M.alba include anti-platelet,anxiolytic,anti-asthmatic,anthelmintic,antidepressant,cardioprotective,and immunomodulatory activities.Clinical trials on the efficiency of M.alba extracts in reducing blood glucose and cholesterol levels and enhancing cognitive ability have been conducted.The phytochemistry and pharmacology of the different parts of the mulberry tree confer its traditional and current uses as fodder,food,cosmetics,and medicine.Overall,M.alba is a multi-functional plant with promising medicinal properties.展开更多
The aim of this study was to associate the generation of reactive oxygen species (ROS) with Induced antloxidant responses and disturbed cellular redox environment in the nitrogen-(N), phosphorus-(P), or potassi...The aim of this study was to associate the generation of reactive oxygen species (ROS) with Induced antloxidant responses and disturbed cellular redox environment in the nitrogen-(N), phosphorus-(P), or potassium-(K) deftcient mulberry (Morus alba L. var. Kanva-2) plants. The indicators of oxidative stress and cellular redox environment and antioxldant defense-related parameters were analyzed. Oeficlency of N, P or K suppressed growth, accelerated senescence, and decreased concentrations of chloroplastic pigments and glutathione. Lipid peroxidation and activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase were also increased in these N, P, or K deprived plants. Concentration of hydrogen peroxide Increased in plants deficient in N or P. Oeficlency of N or P particularly altered the cellular redox environment as indicated by changes in the redox couples, namely ascorbic acid/total ascorbate decreased in P-, glutathione sulfydryl/total glutathione decreased in N-, and Increased in P-deficient plants. Activity staining of native gels for superoxide dismutase revealed Increased activity as Indicated by Increased intensity of bands, and induction of few new isoforms in P- and K-deficient plants. Oifferences in the patterns of superoxide dismutase isoforms and redox status (ascorbic acid/total ascorbate and glutathlone sulfydryl/total glutathione) Indicate that N-, P-, or K-deficiency altered antioxidant responses to varying extents in mulberry plants.展开更多
该文旨在探究桑叶粉[Mulberry(Morus alba L.)Leaf Powder]对中华鳖(Truogx sinensis)生长性能、肝功能及血清抗氧化能力的影响。试验选取120只体重一致的中华鳖幼鳖,随机分为两组,对照组饲喂基础日粮,试验组在基础日粮中添加,试验为期6...该文旨在探究桑叶粉[Mulberry(Morus alba L.)Leaf Powder]对中华鳖(Truogx sinensis)生长性能、肝功能及血清抗氧化能力的影响。试验选取120只体重一致的中华鳖幼鳖,随机分为两组,对照组饲喂基础日粮,试验组在基础日粮中添加,试验为期60 d。结果表明:与对照组相比,试验组中华鳖的体形、内脏形态与颜色均未出现明显差别,内脏与体重比、肝脏与体重比等均无显著性差异(P>0.05);谷草转氨酶(AST)、谷丙转氨酶(ALT)、超氧化物歧化酶活性(T-SOD)、丙二醛(MDA)、谷胱甘肽过氧化酶(GSH-PX)没有显著性差异(P>0.05);而总抗氧化能力(T-AOC)试验组显著低于对照组(P<0.05)。提示饲料添加3%桑叶粉不影响中华鳖的生长性能和肝功能,但影响中华鳖的血清总抗氧化水平。展开更多
文摘This research investigates the mechanical and thermal properties of Morus alba combined with polylactic acid in comparison with other natural fibers. The study uses three different fiber and PLA compositions - 20%, 30%, and 40% respectively - to produce composite materials. In addition, another composite with the same fiber volume is treated with a 4% NaOH solution to improve mechanical properties. The composites are processed by twin-screw extrusion, granulation, and injection molding. Tensile strength measurements of raw fibers and NaOH-treated fibers were carried out using a single-fiber tensile test with a gauge length of 40 mm. It was observed that the NaOH surface treatment increases the resistance against tensile loading and exhibited improved properties for raw fiber strands. The diameter of the fibers was measured using optical microscopy. During this research, flexural tests, impact tests, differential scanning calorimetry (DSC), and heat deflection temperature measurements (HDT) were conducted to evaluate the mechanical and thermal properties of the developed composite samples. The results indicate that the mechanical properties of NaOH-treated Morus alba-reinforced polylactic acid outperform both virgin PLA samples and untreated Morus alba samples.
文摘This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·kg-1 MAEs treatment significantly reduced the NDEA-induced hepatic nodules incidence and hepatocellular carcinoma incidence by 58.30% and 41.70% compared to the model group. Meanwhile, MAEs significantly restored the elevated the liver function enzymes, inhibited the tumor necrosis factor alpha and interleukin-6 levels, elevated the serum interleukin-10 and interferon-γ and increased hepatic glutathione-S-transferase and UDP-glucuronosyltransferase 2B1 enzyme activity. Moreover, 150 mg·kg-1 MAEs supplement enhanced glutathione content and the activities of superoxide dismutase, catalase, glutathione peroxidase activities but reduced the malondialdehyde and thiobarbituric acid-reactive substances content by 37.90% and 44.52%. Furthermore, MAEs pretreatment maintained nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1, heme oxygenase-1, and NAD(P)H: quinine oxidoreductase1 stimulation and inhibited the expression of TNF-α, nuclear factor-kappaB (NF-κB), and cyclooxygenase-2 (COX-2), indicating that MAEs exhibit effectively prevention effects against liver cancer via decreased lipid peroxidation, induced Nrf2-mediated antioxidant enzymes and attenuating the inflammatory mediators COX-2 through NF-κB pathway. Thus, MAEs of mulberry variety PR-01 may be used as a good functional dietary supplement against liver cancer.
文摘为揭示叶果间的内在作用规律,以桑树(Morus alba L.)当年生果枝为研究对象,分析不同大小果枝上叶片特征和果实性状的差异及其二者间的相互关系。结果表明:随着果枝由小到大,果枝上的叶片数与总叶干重显著增加,而叶形指数却无显著变化;桑葚数与总桑葚干重也有显著增加,桑葚含水量无显著变化。桑葚数与叶片数、总叶干重、总叶面积及其平均桑葚干重显著正相关。研究结果证实了果实的性状与叶片特征之间存在极密切关系。当年生果枝上的桑葚数量多少取决于果枝上的叶片总数,平均桑葚的干重则与果枝上的平均叶面积显著相关。
基金Supported by Achievement Cultivation and Talent Training Project of Guizhou Academy of Agricultural Sciences[Qian Nong Ke Yuan CR He Zi(2014)52]
文摘This paper aimed to explore the correlation between Morus alba L. leaf node and its size or mass change and provide a theoretical basis for the development of scientific Morus alba L. cultivation technology. During 2016-2017,the correlation between Morus alba L. leaf node and its size or mass change was investigated and researched. Results showed that through the correlation coefficient analysis,there was a weakly positive correlation between the leaf node and some indicators such as( leaf length ÷ leaf width) and petiole length,there was a weakly negative correlation between the leaf node and some indicators such as leaf width,( leaf length + leaf width),( leaf length × leaf width) and leaf mass,and there was a weakly negative correlation between the leaf node and the other indicators; there was a highly positive correlation between the leaf mass and some indicators such as Morus alba L. leaf length,leaf width,( leaf length + leaf width) and( leaf length × leaf width),there was a significantly positive correlation between the leaf mass and some indicators such as petiole transverse diameter in width and petiole mass,and there was a weakly positive correlation between the leaf mass and some indicators such as petiole transverse diameter in thickness. In terms of significant level of difference,it was found that there was no significant difference in the correlation coefficients between the leaf nodes on the Morus alba L. shoots and some indicators such as leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width) and leaf mass; there was an extremely significant difference in the correlation coefficients between the leaf mass and some indicators such as Morus alba L. leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width),petiole transverse diameter in width and petiole mass; there was no significant difference in the correlation coefficients between the leaf mass and the other indicators. The regression analysis was performed on the leaf node and leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width),( leaf length ÷ leaf width),petiole length,petiole diameter in width,petiole diameter in thickness,petiole mass and leaf mass. Significance F values were0. 4864,0. 1995,0. 2888,0. 3004,0. 2350,0. 2407,0. 8925,0. 5227,0. 7022 and 0. 2000,respectively,that is,there was an extremely significant difference between Morus alba L. leaf node and petiole diameter in width,there was a significant difference between Morus alba L.leaf node and petiole mass,and there was no significant difference between Morus alba L. leaf node and the other indicators. The comparative analysis of quadratic curve regression equation and linear regression equation was performed on some indicators such as leaf length,and it was found that the R value between the leaf node and petiole diameter in width was in line with the logarithmic curve regression equation,and the other R values of quadratic curve regression equation was larger than the other R values of linear regression equation,indicating that different Morus alba L. leaf nodes,leaf length,leaf mass and other indicators were in line with the quadratic curve regression equation. It was concluded that by investigating the leaf node on the Morus alba L. shoots and regression equation,we could predict the leaf size,leaf mass and other indicators,and we could take the leaf node on the Morus alba L. shoots as a main factor affecting the Morus alba L. leaf size and mass.
文摘White mulberry tree (Morus alba L) is cultivated throughout Asia and Europe, including Poland. The leaves and root bark preparations from Morus alba have been used in traditional phytomedicine. The objective of the present study was to compare chemical composition and antioxidative activity of aqueous extracts prepared from Polish variety of Morus alba leaves at the laboratory (L) and pilot plant scale (PP) conditions. Proximate composition, phenolic acids profile (HPLC/MS), flavonol glicosides (HPLC/ MS), polyphenols (Folin-Ciocalteu assay), and the antioxidant activity (ABTS and DPPH assay) of the extracts were determined. The main phenolic compounds were identified as gallic, protocatechuic, phydroxybenzoic, vanillic, chlorogenic, caffeic, p-coumaric, ferulic, and sinapic acids. Chlorogenic acid was the main phenolic constituent of both extracts. The flavonols fraction contained rutin, quercetin 3-β-D-glucoside, and kaempferol 3-β-D- glucopyranoside. Total concentration of phenolic compounds were 7.9 g and 14.4 g gallic acid equivalent/100 g extract, and antioxidant activity was 137.1 and 214.1 μMol Trolox equivalent/g dry weight for the PP and L extracts, respectively. We concluded that current pilot plant process is less efficient than laboratory process at the aqueous extraction of bioactive components from Morus alba dried leaves. Potential improvements may include increasing efficacy of the extraction, decreasing losses of bioactive components during the process, or both.
基金supported by Defence Research and Development Organization (DRDO),Ministry of Defence, Government of India
文摘Ten quantitative morphological characters were studied in 56 Morus alba L. trees representing three natural populations from the trans-Himalayan Ladakh region. The altitude of collection sites ranged from 2815 to 3177 m above the sea level(asl). Coefficient of variation(CV) showed high phenotypic variation in M. alba. Linear regression analysis revealed that leaf and fruit size decreases with an increase in altitude. High CV was observed for leaf length, leaf width, petiole length, leaf area, internodal distance, number of nodes, bud length, fruit length, fruit width and fruit weight. Similarly, a high phenotypic plasticity index was observed for bud length, leaf length, leaf width, petiole length, leaf area, inter-nodal distance, number of nodes, fruit length, fruit width and fruit weight. For every 100 m increase in altitude, leaf length, leaf width and leaf area decreased by 1 cm, 0.8 cm and 16.6 cm2, respectively. Analysis of covariance showed a predominant altitudinal effect on the morphological characters in comparison to the population effect. A small change in the altitude caused significant change in the plant morphological characteristics. The present investigation represents to our knowledge the first study addressing phenotypic variation in mulberryalong an altitudinal gradient.
文摘Morus alba(white mulberry) mesophyll protoplasts were isolated from leaves of 30-45 day old sterile shoots,with protoplast yields of 2.5 x 107 g-1/F.W. after purification. The protoplasts were cultured in a modified K8P liquid medium containing 0.2 mg/L 2,4-D(2,4- Dichlorophe-noxy acetic acid), 1 mg/L NAA(Naphthyl acetic acid) and 0.5 mg/L BA(6-benzylaminopurine). A low plating density (5 x 104/ml) proved to be favourable to the division of protoplast-derived cells. The first divisioll occurred 4 days after culture, and the division frequency reached 24% at 10 days. A number of cell colonies and microcalli formed in 6 weeks. The microcalli were transferred onto MSB medium with 0.5 mg/L NAA and 0.5 mg/L BA for further proliferation. Shoot formation was initiated when the calli of 3-4 mm in size were transferred onto MSB differentiation medium with 0.1 mg/L NAA and 1 mg/L BA. The frequency of shoot formation was 35%. The shoots of 4-5 cm in height were excised from the callus and rooted on half strength MS medium with 0.5 mg/L IBA and 0.1 mg/L BA. After transplantation into pots, the regenerated plants grew vigorously in the phytotron.
文摘The aim of the study was to develop the technology of muesli via analyzing the addition of white mulberry fruit. The first step involved the selection of ingredients from bioactive compounds. Next, the technology of production was developed. Then, the antioxidant activity of new muesli with potentially pro-health model systems was estimated. Muesli was characterized in terms of its basic nutritional value, and antioxidant potential. The ability to inactivate DPPH and ABTS radicals was estimated. In addition, the amount of fat in muesli was calculated. As part of this calculation, fatty acid composition and content of primary and secondary oxidation products were ascertained. The last stage of the project included sensory analysis of the resultant products. Our studies showed that the new product-muesli had high nutritional value, high antioxidant potential and a positive fatty acid composition. And sensory analysis showed that the product was attractive.
文摘The present review is aimed at providing a comprehensive summary on the botany,utility,phytochemistry,pharmacology,and clinical trials of Morus alba(mulberry or sang shu).The mulberry foliage has remained the primary food for silkworms for centuries.Its leaves have also been used as animal feed for livestock and its fruits have been made into a variety of food products.With flavonoids as major constituents,mulberry leaves possess various biological activities,including antioxidant,antimicrobial,skin-whitening,cytotoxic,anti-diabetic,glucosidase inhibition,anti-hyperlipidemic,anti-atherosclerotic,anti-obesity,cardioprotective,and cognitive enhancement activities.Rich in anthocyanins and alkaloids,mulberry fruits have pharmacological properties,such as antioxidant,anti-diabetic,anti-atherosclerotic,anti-obesity,and hepatoprotective activities.The root bark of mulberry,containing flavonoids,alkaloids and stilbenoids,has antimicrobial,skin-whitening,cytotoxic,anti-inflammatory,and anti-hyperlipidemic properties.Other pharmacological properties of M.alba include anti-platelet,anxiolytic,anti-asthmatic,anthelmintic,antidepressant,cardioprotective,and immunomodulatory activities.Clinical trials on the efficiency of M.alba extracts in reducing blood glucose and cholesterol levels and enhancing cognitive ability have been conducted.The phytochemistry and pharmacology of the different parts of the mulberry tree confer its traditional and current uses as fodder,food,cosmetics,and medicine.Overall,M.alba is a multi-functional plant with promising medicinal properties.
基金Supported by the Council of Science and Technology, U.P., Lucknow and the Council of Scientific and Industrial Research (CSIR), New Delhi, India. Publication of this paper is supported by the National Natural Science Foundation of China (30624808) and Science Publication Foundation of the Chinese Academy of Sciences.Acknowledgements We thank Dr P. K. Singh for his assistance and for providing AAS facilities.
文摘The aim of this study was to associate the generation of reactive oxygen species (ROS) with Induced antloxidant responses and disturbed cellular redox environment in the nitrogen-(N), phosphorus-(P), or potassium-(K) deftcient mulberry (Morus alba L. var. Kanva-2) plants. The indicators of oxidative stress and cellular redox environment and antioxldant defense-related parameters were analyzed. Oeficlency of N, P or K suppressed growth, accelerated senescence, and decreased concentrations of chloroplastic pigments and glutathione. Lipid peroxidation and activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase were also increased in these N, P, or K deprived plants. Concentration of hydrogen peroxide Increased in plants deficient in N or P. Oeficlency of N or P particularly altered the cellular redox environment as indicated by changes in the redox couples, namely ascorbic acid/total ascorbate decreased in P-, glutathione sulfydryl/total glutathione decreased in N-, and Increased in P-deficient plants. Activity staining of native gels for superoxide dismutase revealed Increased activity as Indicated by Increased intensity of bands, and induction of few new isoforms in P- and K-deficient plants. Oifferences in the patterns of superoxide dismutase isoforms and redox status (ascorbic acid/total ascorbate and glutathlone sulfydryl/total glutathione) Indicate that N-, P-, or K-deficiency altered antioxidant responses to varying extents in mulberry plants.