The longitudinal dispersion of the projectile in shooting tests of two-dimensional trajectory corrections fused with fixed canards is extremely large that it sometimes exceeds the correction ability of the correction ...The longitudinal dispersion of the projectile in shooting tests of two-dimensional trajectory corrections fused with fixed canards is extremely large that it sometimes exceeds the correction ability of the correction fuse actuator.The impact point easily deviates from the target,and thus the correction result cannot be readily evaluated.However,the cost of shooting tests is considerably high to conduct many tests for data collection.To address this issue,this study proposes an aiming method for shooting tests based on small sample size.The proposed method uses the Bootstrap method to expand the test data;repeatedly iterates and corrects the position of the simulated theoretical impact points through an improved compatibility test method;and dynamically adjusts the weight of the prior distribution of simulation results based on Kullback-Leibler divergence,which to some extent avoids the real data being"submerged"by the simulation data and achieves the fusion Bayesian estimation of the dispersion center.The experimental results show that when the simulation accuracy is sufficiently high,the proposed method yields a smaller mean-square deviation in estimating the dispersion center and higher shooting accuracy than those of the three comparison methods,which is more conducive to reflecting the effect of the control algorithm and facilitating test personnel to iterate their proposed structures and algorithms.;in addition,this study provides a knowledge base for further comprehensive studies in the future.展开更多
In this paper we provide a unified framework for consensus tracking of leader-follower multi-agent systems with measurement noises based on sampled data with a general sampling delay. First, a stochastic bounded conse...In this paper we provide a unified framework for consensus tracking of leader-follower multi-agent systems with measurement noises based on sampled data with a general sampling delay. First, a stochastic bounded consensus tracking protocol based on sampled data with a general sampling delay is presented by employing the delay decomposition technique. Then, necessary and sufficient conditions are derived for guaranteeing leader-follower multi-agent systems with measurement noises and a time-varying reference state to achieve mean square bounded consensus tracking. The obtained results cover no sampling delay, a small sampling delay and a large sampling delay as three special cases. Last, simulations are provided to demonstrate the effectiveness of the theoretical results.展开更多
In this paper, consensus problems of heterogeneous multi-agent systems based on sampled data with a small sampling delay are considered. First, a consensus protocol based on sampled data with a small sampling delay fo...In this paper, consensus problems of heterogeneous multi-agent systems based on sampled data with a small sampling delay are considered. First, a consensus protocol based on sampled data with a small sampling delay for heterogeneous multi-agent systems is proposed. Then, the algebra graph theory, the matrix method, the stability theory of linear systems, and some other techniques are employed to derive the necessary and sufficient conditions guaranteeing heterogeneous multi-agent systems to asymptotically achieve the stationary consensus. Finally, simulations are performed to demonstrate the correctness of the theoretical results.展开更多
The bounded consensus tracking problems of second-order multi-agent systems under directed networks with sam- pling delay are addressed in this paper. When the sampling delay is more than a sampling period, new protoc...The bounded consensus tracking problems of second-order multi-agent systems under directed networks with sam- pling delay are addressed in this paper. When the sampling delay is more than a sampling period, new protocols based on sampled-data control are proposed so that each agent can track the time-varying reference state of the virtual leader. By using the delay decomposition approach, the augmented matrix method, and the frequency domain analysis, necessary and sufficient conditions are obtained, which guarantee that the bounded consensus tracking is realized. Furthermore, some numerical simulations are presented to demonstrate the effectiveness of the theoretical results.展开更多
This paper considers blind chip rate estimation of DS-SS signals in multi-rate and multi-user DS-CDMA systems over channels having slow flat Rayleigh fading plus additive white Gaussian noise. Channel impulse response...This paper considers blind chip rate estimation of DS-SS signals in multi-rate and multi-user DS-CDMA systems over channels having slow flat Rayleigh fading plus additive white Gaussian noise. Channel impulse response is estimated by a subspace method, and then the chip rate of each signal is estimated using zero crossing of estimated differential channel impulse response. For chip rate estimation of each user, an algorithm which uses weighted zero-crossing ratio is proposed. Maximum value of the weighted zero crossing ratio takes place in the Nyquist rate sampling frequency, which equals to the twice of the chip rate. Furthermore, bit time of each user is estimated using fluctuations of autocorrelation estimators. Since code length of each user can be obtained using bit time and chip time ratio. Fading channels reduce reliability factor of the proposed algo-rithm. To overcome this problem, a receiver with multiple antennas is proposed, and the reliability factor of the proposed algorithm is analyzed over both spatially correlated and independent fading channels.展开更多
In the 6th edition of the Chinese Space Trajectory Design Competition held in 2014, a near-Earth asteroid sample-return trajectory design problem was released, in which the motion of the spacecraft is modeled in multi...In the 6th edition of the Chinese Space Trajectory Design Competition held in 2014, a near-Earth asteroid sample-return trajectory design problem was released, in which the motion of the spacecraft is modeled in multi-body dynamics, considering the gravitational forces of the Sun, Earth, and Moon. It is proposed that an electric-propulsion spacecraft initially parking in a circular 200-kin-altitude low Earth orbit is expected to rendezvous with an asteroid and carry as much sample as possible back to the Earth in a 10-year time frame. The team from the Technology and Engineering Center for Space Utilization, Chinese Academy of Sciences has reported a solution with an asteroid sample mass of 328 tons, which is ranked first in the competition. In this article, we will present our design and optimization methods, primarily including overall analysis, target selection, escape from and capture by the Earth-Moon system, and optimization of impulsive and low-thrust trajectories that are modeled in multi-body dynamics. The orbital resonance concept and lunar gravity assists are considered key techniques employed for trajectory design. The reported solution, preliminarily revealing the feasibility of returning a hundreds-of-tons asteroid or asteroid sample, envisions future space missions relating to near-Earth asteroid exploration.展开更多
Multiple Uninhabited Aerial Vehicles (multi-UAVs) coordinated trajectory replanning is one of the most complicated global optimum problems in multi-UAVs coordinated control. Based on the construction of the basic mode...Multiple Uninhabited Aerial Vehicles (multi-UAVs) coordinated trajectory replanning is one of the most complicated global optimum problems in multi-UAVs coordinated control. Based on the construction of the basic model of multi-UAVs coordinated trajectory replanning, which includes problem description, threat modeling, constraint conditions, coordinated function and coordination mechanism, a novel Max-Min adaptive Ant Colony Optimization (ACO) approach is presented in detail. In view of the characteristics of multi-UAVs coordinated trajectory replanning in dynamic and uncertain environments, the minimum and maximum pheromone trails in ACO are set to enhance the searching capability, and the point pheromone is adopted to achieve the collision avoidance between UAVs at the trajectory planner layer. Considering the simultaneous arrival and the air-space collision avoidance, an Estimated Time of Arrival (ETA) is decided first. Then the trajectory and flight velocity of each UAV are determined. Simulation experiments are performed under the complicated combating environment containing some static threats and popup threats. The results demonstrate the feasibility and the effectiveness of the proposed approach.展开更多
Aiming at increasing the calculation efficiency of the pseudospectral methods, a multiple- interval Radau pseudospectral method (RPM) is presented to generate a reusable launch vehicle (RLV) 's optimal re-entry t...Aiming at increasing the calculation efficiency of the pseudospectral methods, a multiple- interval Radau pseudospectral method (RPM) is presented to generate a reusable launch vehicle (RLV) 's optimal re-entry trajectory. After dividing the optimal control problem into many intervals, the state and control variables are approximated using many fixed- and low-degree Lagrange polyno- mials in each interval. Convergence of the numerical discretization is then achieved by increasing the number of intervals. With the application of the proposed method, the normal nonlinear program- ming (NLP) problem transcribed from the optimal control problem can avoid being dense because of the low-degree approximation polynomials in each interval. Thus, the NLP solver can easily compute a solution. Finally, simulation results show that the optimized re-entry trajectories satisfy the path constraints and the boundary constraints successfully. Compared with the single interval RPM, the multiple-interval RPM is significantly faster and has higher calculation efficiency. The results indicate that the multiple-interval RPM can be applied for real-time trajectory generation due to its high effi- ciency and high precision.展开更多
In the constrained reentry trajectory design of hypersonic vehicles, multiple objectives with priorities bring about more difficulties to find the optimal solution. Therefore, a multi-objective reentry trajectory opti...In the constrained reentry trajectory design of hypersonic vehicles, multiple objectives with priorities bring about more difficulties to find the optimal solution. Therefore, a multi-objective reentry trajectory optimization (MORTO) approach via generalized varying domain (GVD) is proposed. Using the direct collocation approach, the trajectory optimization problem involving multiple objectives is discretized into a nonlinear multi-objective programming with priorities. In terms of fuzzy sets, the objectives are fuzzified into three types of fuzzy goals, and their constant tolerances are substituted by the varying domains. According to the principle that the objective with higher priority has higher satisfactory degree, the priority requirement is modeled as the order constraints of the varying domains. The corresponding two-side, single-side, and hybrid-side varying domain models are formulated for three fuzzy relations respectively. By regulating the parameter, the optimal reentry trajectory satisfying priorities can be achieved. Moreover, the performance about the parameter is analyzed, and the algorithm to find its specific value for maximum priority difference is proposed. The simulations demonstrate the effectiveness of the proposed method for hypersonic vehicles, and the comparisons with the traditional methods and sensitivity analysis are presented.展开更多
Molecular microbiological methods, such as competetive PCR, real-time PCR, denaturing gradient gel electrophoresis (DGGE) and large-scale parallel-pyrosequencing, require the extraction of sufficient quantity of high ...Molecular microbiological methods, such as competetive PCR, real-time PCR, denaturing gradient gel electrophoresis (DGGE) and large-scale parallel-pyrosequencing, require the extraction of sufficient quantity of high quality DNA from microbiologically and chemically complex matrices. Due to difficulties in the field to standardize/select the optimum DNA preservation-extraction methods in view of laboratories differences, this article attempts to present a straight-forward mathematical framework for comparing some of the most commonly used methods. To this end, as a case study, the problem of selecting an optimum sample preservation-DNA extraction strategy for obtaining total bacterial DNA from swine feces was considered. Two sample preservation methods (liquid nitrogen and RNAlater?) and seven extraction techniques were paired and compared under six quantitative DNA analysis criteria: yield of extraction, purity of extracted DNA (A260/280 and A 260/230 ratios), duration of extraction, degradation degree of DNA, and cost. From a practical point of view, it is unlikely that a single sample preservation-DNA extraction strategy can be optimum for all selected criteria. Hence, a systematic multi-criteria decision-making (MCDM) approach was used to compare the methods. As a result, the ZR Fecal DNA MiniPrepTM DNA extraction kit for samples preserved either with liquid nitrogen or RNAlater? were identified as potential optimum solutions for obtaining total bacterial DNA from swine feces. Considering the need for practicality for in situ applications, we would recommend liquid nitrogen as sample preservation method, along with the ZR Fecal DNA MiniPrepTM kit. Total bacterial DNA obtained by this strategy can be suitable for downstream PCR-based DNA analyses of swine feces.展开更多
In recent years, the modern methods of multi-element analysis of precious metals have attracted wide attention in scientific research and industry. The application and development in the decomposition of samples, sepa...In recent years, the modern methods of multi-element analysis of precious metals have attracted wide attention in scientific research and industry. The application and development in the decomposition of samples, separation and enrichment, and modern instrumental analysis of the platinum-group elements (PGEs) and gold in geological and environmental samples have been reviewed. Finally, the tendency of analysis of precious metals is also prospected.展开更多
This paper is concerned with the stochastic bounded consensus tracking problems of leader-follower multi-agent systems, where the control input of an agent can only use the information measured at the sampling instant...This paper is concerned with the stochastic bounded consensus tracking problems of leader-follower multi-agent systems, where the control input of an agent can only use the information measured at the sampling instants from its neighbours or the virtual leader with a time-varying reference state, and the measurements are corrupted by random noises. The probability limit theory and the algebra graph theory are employed to derive the necessary and sufficient conditions guaranteeing the mean square bounded consensus tracking. It is shown that the maximum allowable upper boundary of the sampling period simultaneously depends on the constant feedback gains and the network topology. Furthermore, the effects of the sampling period on the tracking performance are analysed. It turns out that from the view point of the sampling period, there is a trade-off between the tracking speed and the static tracking error. Simulations are provided to demonstrate the effectiveness of the theoretical results.展开更多
This paper considers the problem of generating a flight trajectory for a single fixed-wing unmanned combat aerial vehicle (UCAV) performing an air-to-surface multi-target attack (A/SMTA) mission using satellite-gu...This paper considers the problem of generating a flight trajectory for a single fixed-wing unmanned combat aerial vehicle (UCAV) performing an air-to-surface multi-target attack (A/SMTA) mission using satellite-guided bombs. First, this problem is formulated as a variant of the traveling salesman problem (TSP), called the dynamic-constrained TSP with neighborhoods (DCT- SPN). Then, a hierarchical hybrid approach, which partitions the planning algorithm into a roadmap planning layer and an optimal control layer, is proposed to solve the DCTSPN. In the roadmap planning layer, a novel algorithm based on an updatable proba- bilistic roadmap (PRM) is presented, which operates by randomly sampling a finite set of vehicle states from continuous state space in order to reduce the complicated trajectory planning problem to planning on a finite directed graph. In the optimal control layer, a collision-free state-to-state trajectory planner based on the Gauss pseudospectral method is developed, which can generate both dynamically feasible and optimal flight trajectories. The entire process of solving a DCTSPN consists of two phases. First, in the offline preprocessing phase, the algorithm constructs a PRM, and then converts the original problem into a standard asymmet- ric TSP (ATSP). Second, in the online querying phase, the costs of directed edges in PRM are updated first, and a fast heuristic searching algorithm is then used to solve the ATSP. Numerical experiments indicate that the algorithm proposed in this paper can generate both feasible and near-optimal solutions quickly for online purposes.展开更多
Homing trajectory planning is a core task of autonomous homing of parafoil system.This work analyzes and establishes a simplified kinematic mathematical model,and regards the homing trajectory planning problem as a ki...Homing trajectory planning is a core task of autonomous homing of parafoil system.This work analyzes and establishes a simplified kinematic mathematical model,and regards the homing trajectory planning problem as a kind of multi-objective optimization problem.Being different from traditional ways of transforming the multi-objective optimization into a single objective optimization by weighting factors,this work applies an improved non-dominated sorting genetic algorithm Ⅱ(NSGA Ⅱ) to solve it directly by means of optimizing multi-objective functions simultaneously.In the improved NSGA Ⅱ,the chaos initialization and a crowding distance based population trimming method were introduced to overcome the prematurity of population,the penalty function was used in handling constraints,and the optimal solution was selected according to the method of fuzzy set theory.Simulation results of three different schemes designed according to various practical engineering requirements show that the improved NSGA Ⅱ can effectively obtain the Pareto optimal solution set under different weighting with outstanding convergence and stability,and provide a new train of thoughts to design homing trajectory of parafoil system.展开更多
基金the National Natural Science Foundation of China(Grant No.61973033)Preliminary Research of Equipment(Grant No.9090102010305)for funding the experiments。
文摘The longitudinal dispersion of the projectile in shooting tests of two-dimensional trajectory corrections fused with fixed canards is extremely large that it sometimes exceeds the correction ability of the correction fuse actuator.The impact point easily deviates from the target,and thus the correction result cannot be readily evaluated.However,the cost of shooting tests is considerably high to conduct many tests for data collection.To address this issue,this study proposes an aiming method for shooting tests based on small sample size.The proposed method uses the Bootstrap method to expand the test data;repeatedly iterates and corrects the position of the simulated theoretical impact points through an improved compatibility test method;and dynamically adjusts the weight of the prior distribution of simulation results based on Kullback-Leibler divergence,which to some extent avoids the real data being"submerged"by the simulation data and achieves the fusion Bayesian estimation of the dispersion center.The experimental results show that when the simulation accuracy is sufficiently high,the proposed method yields a smaller mean-square deviation in estimating the dispersion center and higher shooting accuracy than those of the three comparison methods,which is more conducive to reflecting the effect of the control algorithm and facilitating test personnel to iterate their proposed structures and algorithms.;in addition,this study provides a knowledge base for further comprehensive studies in the future.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61203147,60973095,60804013,and 61104092)the Fundamental Research Funds for the Central Universities,China(Grant Nos.JUSRP111A44,JUSRP21011,and JUSRP11233)+1 种基金the Foundation of State Key Laboratory of Digital Manufacturing Equipment and Technology,HUST,China(Grant No.DMETKF2010008)the Humanities and Social Sciences Youth Funds of the Ministry of Education,China(Grant No.12YJCZH218)
文摘In this paper we provide a unified framework for consensus tracking of leader-follower multi-agent systems with measurement noises based on sampled data with a general sampling delay. First, a stochastic bounded consensus tracking protocol based on sampled data with a general sampling delay is presented by employing the delay decomposition technique. Then, necessary and sufficient conditions are derived for guaranteeing leader-follower multi-agent systems with measurement noises and a time-varying reference state to achieve mean square bounded consensus tracking. The obtained results cover no sampling delay, a small sampling delay and a large sampling delay as three special cases. Last, simulations are provided to demonstrate the effectiveness of the theoretical results.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61203147,61374047,61203126,and 61104092)the Humanities and Social Sciences Youth Funds of the Ministry of Education,China(Grant No.12YJCZH218)
文摘In this paper, consensus problems of heterogeneous multi-agent systems based on sampled data with a small sampling delay are considered. First, a consensus protocol based on sampled data with a small sampling delay for heterogeneous multi-agent systems is proposed. Then, the algebra graph theory, the matrix method, the stability theory of linear systems, and some other techniques are employed to derive the necessary and sufficient conditions guaranteeing heterogeneous multi-agent systems to asymptotically achieve the stationary consensus. Finally, simulations are performed to demonstrate the correctness of the theoretical results.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.60874053 and 61034006)
文摘The bounded consensus tracking problems of second-order multi-agent systems under directed networks with sam- pling delay are addressed in this paper. When the sampling delay is more than a sampling period, new protocols based on sampled-data control are proposed so that each agent can track the time-varying reference state of the virtual leader. By using the delay decomposition approach, the augmented matrix method, and the frequency domain analysis, necessary and sufficient conditions are obtained, which guarantee that the bounded consensus tracking is realized. Furthermore, some numerical simulations are presented to demonstrate the effectiveness of the theoretical results.
文摘This paper considers blind chip rate estimation of DS-SS signals in multi-rate and multi-user DS-CDMA systems over channels having slow flat Rayleigh fading plus additive white Gaussian noise. Channel impulse response is estimated by a subspace method, and then the chip rate of each signal is estimated using zero crossing of estimated differential channel impulse response. For chip rate estimation of each user, an algorithm which uses weighted zero-crossing ratio is proposed. Maximum value of the weighted zero crossing ratio takes place in the Nyquist rate sampling frequency, which equals to the twice of the chip rate. Furthermore, bit time of each user is estimated using fluctuations of autocorrelation estimators. Since code length of each user can be obtained using bit time and chip time ratio. Fading channels reduce reliability factor of the proposed algo-rithm. To overcome this problem, a receiver with multiple antennas is proposed, and the reliability factor of the proposed algorithm is analyzed over both spatially correlated and independent fading channels.
基金Supported by National Basic Research Program of China (973 Program) (2010CB731800), Key Project of Natural Science Fouudation of China (60934003), National Natural Science Foundation of China (61074065, 60974018), Natural Science Foundation of Hebei Province(F2012203119), and the Science Foundation of Yanshan University for the Excellent Ph. D. Students (201204) The authors thank Chen Cai-Lian of the Shanghai Jiao Tong University for her comments on English polishing and problem formulation.
基金supported by the National Natural Science Foundation of China(Grant11372311)the grant from the State key Laboratory of Astronautic Dynamics(2014-ADL-DW0201)
文摘In the 6th edition of the Chinese Space Trajectory Design Competition held in 2014, a near-Earth asteroid sample-return trajectory design problem was released, in which the motion of the spacecraft is modeled in multi-body dynamics, considering the gravitational forces of the Sun, Earth, and Moon. It is proposed that an electric-propulsion spacecraft initially parking in a circular 200-kin-altitude low Earth orbit is expected to rendezvous with an asteroid and carry as much sample as possible back to the Earth in a 10-year time frame. The team from the Technology and Engineering Center for Space Utilization, Chinese Academy of Sciences has reported a solution with an asteroid sample mass of 328 tons, which is ranked first in the competition. In this article, we will present our design and optimization methods, primarily including overall analysis, target selection, escape from and capture by the Earth-Moon system, and optimization of impulsive and low-thrust trajectories that are modeled in multi-body dynamics. The orbital resonance concept and lunar gravity assists are considered key techniques employed for trajectory design. The reported solution, preliminarily revealing the feasibility of returning a hundreds-of-tons asteroid or asteroid sample, envisions future space missions relating to near-Earth asteroid exploration.
基金supported by the Natural Science Foundation of China (Grant no.60604009)Aeronautical Science Foundation of China (Grant no.2006ZC51039,Beijing NOVA Program Foundation of China (Grant no.2007A017)+1 种基金Open Fund of the Provincial Key Laboratory for Information Processing Technology,Suzhou University (Grant no KJS0821)"New Scientific Star in Blue Sky"Talent Program of Beihang University of China
文摘Multiple Uninhabited Aerial Vehicles (multi-UAVs) coordinated trajectory replanning is one of the most complicated global optimum problems in multi-UAVs coordinated control. Based on the construction of the basic model of multi-UAVs coordinated trajectory replanning, which includes problem description, threat modeling, constraint conditions, coordinated function and coordination mechanism, a novel Max-Min adaptive Ant Colony Optimization (ACO) approach is presented in detail. In view of the characteristics of multi-UAVs coordinated trajectory replanning in dynamic and uncertain environments, the minimum and maximum pheromone trails in ACO are set to enhance the searching capability, and the point pheromone is adopted to achieve the collision avoidance between UAVs at the trajectory planner layer. Considering the simultaneous arrival and the air-space collision avoidance, an Estimated Time of Arrival (ETA) is decided first. Then the trajectory and flight velocity of each UAV are determined. Simulation experiments are performed under the complicated combating environment containing some static threats and popup threats. The results demonstrate the feasibility and the effectiveness of the proposed approach.
文摘Aiming at increasing the calculation efficiency of the pseudospectral methods, a multiple- interval Radau pseudospectral method (RPM) is presented to generate a reusable launch vehicle (RLV) 's optimal re-entry trajectory. After dividing the optimal control problem into many intervals, the state and control variables are approximated using many fixed- and low-degree Lagrange polyno- mials in each interval. Convergence of the numerical discretization is then achieved by increasing the number of intervals. With the application of the proposed method, the normal nonlinear program- ming (NLP) problem transcribed from the optimal control problem can avoid being dense because of the low-degree approximation polynomials in each interval. Thus, the NLP solver can easily compute a solution. Finally, simulation results show that the optimized re-entry trajectories satisfy the path constraints and the boundary constraints successfully. Compared with the single interval RPM, the multiple-interval RPM is significantly faster and has higher calculation efficiency. The results indicate that the multiple-interval RPM can be applied for real-time trajectory generation due to its high effi- ciency and high precision.
基金Supported by the National Natural Science Foundation of China (No. 61075065,60774045, U1134108) and the Ph. D Programs Foundation of Ministry of Education of China ( No. 20110162110041 ).
基金supported by the Natural Science Foundation of Tianjin(12JCZDJC30300)the Research Foundation of Tianjin Key Laboratory of Process Measurement and Control(TKLPMC-201613)the State Scholarship Fund of China
文摘In the constrained reentry trajectory design of hypersonic vehicles, multiple objectives with priorities bring about more difficulties to find the optimal solution. Therefore, a multi-objective reentry trajectory optimization (MORTO) approach via generalized varying domain (GVD) is proposed. Using the direct collocation approach, the trajectory optimization problem involving multiple objectives is discretized into a nonlinear multi-objective programming with priorities. In terms of fuzzy sets, the objectives are fuzzified into three types of fuzzy goals, and their constant tolerances are substituted by the varying domains. According to the principle that the objective with higher priority has higher satisfactory degree, the priority requirement is modeled as the order constraints of the varying domains. The corresponding two-side, single-side, and hybrid-side varying domain models are formulated for three fuzzy relations respectively. By regulating the parameter, the optimal reentry trajectory satisfying priorities can be achieved. Moreover, the performance about the parameter is analyzed, and the algorithm to find its specific value for maximum priority difference is proposed. The simulations demonstrate the effectiveness of the proposed method for hypersonic vehicles, and the comparisons with the traditional methods and sensitivity analysis are presented.
文摘Molecular microbiological methods, such as competetive PCR, real-time PCR, denaturing gradient gel electrophoresis (DGGE) and large-scale parallel-pyrosequencing, require the extraction of sufficient quantity of high quality DNA from microbiologically and chemically complex matrices. Due to difficulties in the field to standardize/select the optimum DNA preservation-extraction methods in view of laboratories differences, this article attempts to present a straight-forward mathematical framework for comparing some of the most commonly used methods. To this end, as a case study, the problem of selecting an optimum sample preservation-DNA extraction strategy for obtaining total bacterial DNA from swine feces was considered. Two sample preservation methods (liquid nitrogen and RNAlater?) and seven extraction techniques were paired and compared under six quantitative DNA analysis criteria: yield of extraction, purity of extracted DNA (A260/280 and A 260/230 ratios), duration of extraction, degradation degree of DNA, and cost. From a practical point of view, it is unlikely that a single sample preservation-DNA extraction strategy can be optimum for all selected criteria. Hence, a systematic multi-criteria decision-making (MCDM) approach was used to compare the methods. As a result, the ZR Fecal DNA MiniPrepTM DNA extraction kit for samples preserved either with liquid nitrogen or RNAlater? were identified as potential optimum solutions for obtaining total bacterial DNA from swine feces. Considering the need for practicality for in situ applications, we would recommend liquid nitrogen as sample preservation method, along with the ZR Fecal DNA MiniPrepTM kit. Total bacterial DNA obtained by this strategy can be suitable for downstream PCR-based DNA analyses of swine feces.
文摘In recent years, the modern methods of multi-element analysis of precious metals have attracted wide attention in scientific research and industry. The application and development in the decomposition of samples, separation and enrichment, and modern instrumental analysis of the platinum-group elements (PGEs) and gold in geological and environmental samples have been reviewed. Finally, the tendency of analysis of precious metals is also prospected.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61203147,60973095,60804013,and 61104092)the Fundamental Research Funds for the Central Universities,China(Grant Nos.JUSRP111A44,JUSRP21011, and JUSRP11233)+1 种基金the Foundation of State Key Laboratory of Digital Manufacturing Equipment and Technology,Huazhong University of Science and Technology(HUST),China(Grant No.DMETKF2010008)the Humanities and Social Sciences Youth Funds of the Ministry of Education,China(Grant No.12YJCZH218)
文摘This paper is concerned with the stochastic bounded consensus tracking problems of leader-follower multi-agent systems, where the control input of an agent can only use the information measured at the sampling instants from its neighbours or the virtual leader with a time-varying reference state, and the measurements are corrupted by random noises. The probability limit theory and the algebra graph theory are employed to derive the necessary and sufficient conditions guaranteeing the mean square bounded consensus tracking. It is shown that the maximum allowable upper boundary of the sampling period simultaneously depends on the constant feedback gains and the network topology. Furthermore, the effects of the sampling period on the tracking performance are analysed. It turns out that from the view point of the sampling period, there is a trade-off between the tracking speed and the static tracking error. Simulations are provided to demonstrate the effectiveness of the theoretical results.
文摘This paper considers the problem of generating a flight trajectory for a single fixed-wing unmanned combat aerial vehicle (UCAV) performing an air-to-surface multi-target attack (A/SMTA) mission using satellite-guided bombs. First, this problem is formulated as a variant of the traveling salesman problem (TSP), called the dynamic-constrained TSP with neighborhoods (DCT- SPN). Then, a hierarchical hybrid approach, which partitions the planning algorithm into a roadmap planning layer and an optimal control layer, is proposed to solve the DCTSPN. In the roadmap planning layer, a novel algorithm based on an updatable proba- bilistic roadmap (PRM) is presented, which operates by randomly sampling a finite set of vehicle states from continuous state space in order to reduce the complicated trajectory planning problem to planning on a finite directed graph. In the optimal control layer, a collision-free state-to-state trajectory planner based on the Gauss pseudospectral method is developed, which can generate both dynamically feasible and optimal flight trajectories. The entire process of solving a DCTSPN consists of two phases. First, in the offline preprocessing phase, the algorithm constructs a PRM, and then converts the original problem into a standard asymmet- ric TSP (ATSP). Second, in the online querying phase, the costs of directed edges in PRM are updated first, and a fast heuristic searching algorithm is then used to solve the ATSP. Numerical experiments indicate that the algorithm proposed in this paper can generate both feasible and near-optimal solutions quickly for online purposes.
基金Project(61273138)supported by the National Natural Science Foundation of ChinaProject(14JCZDJC39300)supported by the Key Fund of Tianjin,China
文摘Homing trajectory planning is a core task of autonomous homing of parafoil system.This work analyzes and establishes a simplified kinematic mathematical model,and regards the homing trajectory planning problem as a kind of multi-objective optimization problem.Being different from traditional ways of transforming the multi-objective optimization into a single objective optimization by weighting factors,this work applies an improved non-dominated sorting genetic algorithm Ⅱ(NSGA Ⅱ) to solve it directly by means of optimizing multi-objective functions simultaneously.In the improved NSGA Ⅱ,the chaos initialization and a crowding distance based population trimming method were introduced to overcome the prematurity of population,the penalty function was used in handling constraints,and the optimal solution was selected according to the method of fuzzy set theory.Simulation results of three different schemes designed according to various practical engineering requirements show that the improved NSGA Ⅱ can effectively obtain the Pareto optimal solution set under different weighting with outstanding convergence and stability,and provide a new train of thoughts to design homing trajectory of parafoil system.