Combinational therapy with Myc decoy oligodeoxynucleotides encapsulated in nanocarrier and X-irradiation on breast cancer cells

The Myc gene is the essential oncogene in triple-negative breast cancer(TNBC).This study investigates the synergistic effects of combining Myc decoy oligodeoxynucleotides-encapsulated niosomes-selenium hybrid nanocarriers with X-irradiation exposure on the MDA-MB-468 cell line.Decoy and scramble ODNs for Myc transcription factor were designed and synthesized based on promoter sequences of the Bcl2 gene.The nanocarriers were synthesized by loading Myc ODNs and selenium into chitosan(Chi-Se-DEC),which was then encapsulated in niosome-nanocarriers(NISM@Chi-Se-DEC).FT-IR,DLS,FESEM,and hemolysis tests were applied to confirm its characterization and physicochemical properties.Moreover,cellular uptake,cellular toxicity,apoptosis,cell cycle,and scratch repair assays were performed to evaluate its anticancer effects on cancer cells.All anticancer assessments were repeated under X-ray irradiation conditions(fractionated 2Gy).Physicochemical characteristics of niosomes containing SeNPs and ODNs showed that it is synthesized appropriately.It revealed that the anticancer effect of NISM@Chi-Se-DEC can be significantly improved in combination with X-ray irradiation treatment.It can be concluded that NISM@Chi-Se-DEC nanocarriers have the potential as a therapeutic agent for cancer treatment,particularly in combination with radiation therapy and in-vivo experiments are necessary to confirm the efficacy of this nano-drug.

A Frequency Domain Based Signal Combining Method for Distributed Antenna Arraying

Distributed antenna arraying is a promising technology for weak signal reception. The received signals from different antennas are aligned and combined to improve the receiving signal-to-noise ratio(SNR). However, the combining performance is serious degraded by the difference of sampling frequency between antennas. In this paper, a frequency domain based signal combining method is proposed to solve this problem. The unaligned sampled data in time domain of the received signals are transformed to frequency domain using fast Fourier transform(FFT). The received signals can be aligned in frequency domain when their spectrum resolutions are the same. Therefore the received signals with the same total sampling time can be aligned and combined in frequency domain and then the combined signal is recovered using inverse fast Fourier transform(IFFT). Numerical simulations with two typical modulation types, i.e., PSK and PCM/BPSK/PM, prove the validity and robustness of this method.

Research on Data Combination for Phased-Array Ground Penetrating Radar

To resolve the data combination of Phased-array Ground Penetrating Radar (PAGPR), we first build a model of PAGPR and a layered model, and then a new data combination algorithm is presented based on it. This method calculates time delay of multi-receivers, basing on the signal of the nearest receiver, then shifts other signals and adds them up, and gets one signal at last. It has been proved that this method can restrain noise, multiple waves, clutter waves and improve the precision of time location. In the end, an example is given to prove the method's efficiency.

Developing high-efficiency base editors by combining optimized synergistic core components with new types of nuclear localization signal peptide

The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.

A weighted averaging method for signal probability of logic circuit combined with reconvergent fan-out structures

By analyzing the structures of circuits,a novel approach for signal probability estimation of very large-scale integration(VLSI)based on the improved weighted averaging algorithm(IWAA)is proposed.Considering the failure probability of the gate,first,the first reconvergent fan-ins corresponding to the reconvergent fan-outs were identified to locate the important signal correlation nodes based on the principle of homologous signal convergence.Secondly,the reconvergent fan-in nodes of the multiple reconverging structure in the circuit were identified by the sensitization path to determine the interference sources to the signal probability calculation.Then,the weighted signal probability was calculated by combining the weighted average approach to correct the signal probability.Finally,the reconvergent fan-out was quantified by the mixed-calculation strategy of signal probability to reduce the impact of multiple reconvergent fan-outs on the accuracy.Simulation results on ISCAS85 benchmarks circuits show that the proposed method has approximate linear time-space consumption with the increase in the number of the gate,and its accuracy is 4.2%higher than that of the IWAA.

A Comparative Study on the Excitation of Large Volume Airgun Source with Different Combinations in Hutubi,Xinjiang,China

In order to study the excitation of large-volume airgun source with different combinations in Hutubi,Xinjiang,China,we conducted a targeted experiment.The method of timefrequency analysis is used to study the signals recorded by a seismometer on the shore of the excited pool,and it is concluded that different gun combinations will lead to different frequency of bubble pulse signals.Besides,linear combination method is used to analyze the signal-to-noise ratios of signals excited by different gun combinations which was recorded by seismic stations around the airgun source.In order to improve the signal-tonoise ratios,it is more effective to increase the activation energy(the number of excited guns at the same time)than to stack the excited signals with smaller energy repeatedly.

新工科背景下专业实习体系探索与实践——以轨道交通信号与控制专业为例

为适应新工科对人才培养的新要求,针对轨道交通信号与控制专业的实习体系在实践过程中存在的主要问题,通过深化产教融合、强化虚实结合、细化考评综合,建立纵向“三阶段”逐级递进、横向“双形式”互补的实习新模式,形成“一平台,双引擎,三大素质”的实习教学新理念,构建“一核心,三过程,九指标”的综合能力评价新机制。在实习组织形式、实习资源整合、实习评价方式等方面进行积极探索和大胆实践,对其他工科专业的实习教学体系建立有一定借鉴和参考意义。

基于GEO数据库联合网络药理学研究川芎-赤芍药对治疗动脉粥样硬化的药理过程及分子机制

目的:探讨活血化瘀中药药对川芎-赤芍拮抗动脉粥样硬化的潜在分子机制及药理过程。方法:使用R语言Limma包分析GEO数据库GSE43292数据集,对有表达差异的动脉粥样硬化基因进行筛选和提取。川芎-赤芍药对所含的化学活性组分及靶点通过中药系统药理学数据库与分析平台检索。合并差异基因和药物作用靶点以获得共同的靶点。利用在线分析工具STRING和Cytoscape构建药物和靶点的调控网络以及靶点间的蛋白质-蛋白质相互作用(PPI)网络。然后利用R语言注释靶点基因的基因本体(GO)功能,分析京都基因与基因组百科全书(KEGG)通路,再进行基因集富集分析(GSEA)以验证KEGG的通路和富集的情况,确定靶点基因的调控功能和参与基因调控功能的信号转导通道。结果:共筛选出动脉粥样硬化差异基因1244个,川芎-赤芍药对含36个生物活性成分,其中靶点为环加氧酶1、肾上腺素受体、过氧化物酶体增殖物激活受体-γ、激酶插入域受体、孕激素受体、基质金属蛋白酶9、CXC趋化因子配体8、蛋白激酶Cβ、白细胞介素6、CD14、二肽基肽酶-4、PIK3CG、肾上腺素受体α1B、微管相关蛋白2、尿激酶纤溶酶原激活剂和单胺氧化酶B。靶点在GO中主要富集在合成DNA过程的调控、DNA生物合成的过程、含胶原的细胞外基质、细胞外基质、蛋白酶结合、细胞迁移、血管形成过程、膜筏结构、转录的调节等与动脉粥样硬化炎症、脂肪代谢及血管生成相关的生物学注释。脂质与动脉粥样硬化通路和核因子κB信号通路与动脉粥样硬化关系最为密切,并且在KEGG信号通路和GSEA均显示出富集。结论:川芎-赤芍药对通过潜在的13个活性成分作用于可能的16个靶点调控相关信号转导通路,通过抗炎、调脂和保护血管等方式产生抗动脉粥样硬化的作用。

“数字信号处理”课程中的以渔易鱼

“数字信号处理”是信息类专业中的一门主干基础课,但在教学过程中,经常会遇到学生学习效果不佳、学习动力不足等问题。为了改善这些现状,结合五邑大学实际情况,从教学内容、教学方式、仿真实践等三个方面进行了改革探索,目的是通过理论与实际的结合改善该课程的学习现状,强调知识点的掌握,更重视培养学生的工科学习思维。期望通过以渔易鱼的方式,拓宽学生的工程视野,提高学生的学习兴趣,培养工程的思维方式。

三维多回波聚合梯度回波序列在腰骶丛成像中的初步应用

目的 探讨3D多回波聚合梯度回波序列(3D GEMEC)在腰骶丛神经成像中的应用价值。方法 回顾性分析2022年8~9月六安市人民医院以腰腿疼为主诉的32例住院患者的影像学资料,男15例,女17例,年龄28~78(60.1±15.8)岁。在不同1.5T平台上分别完成腰骶丛3D GEMEC序列和三维T2加权快速梯度回波(3D T2 FFE)序列,比较两种序列腰骶丛神经的信噪比、对比噪声比和主观显示效果。结果 3D GEMEC序列腰骶丛神经的信噪比、对比噪声比以及主观显示效果与3D T2 FFE序列的差异均无统计学意义(P=0.250、0.146、0.083)。结论 3D GEMEC序列可以清晰地显示腰骶丛神经的解剖结构并抑制背景信号,在腰骶丛神经病变的诊断方面具有一定的临床应用前景。

试验环境水下声信号的特征提取方法

水下试验环境参数的反演是水声学研究领域的重要内容。而当前研究的关键是通过对水下声信号做特征提取从而获取参数信息。针对特征提取较难、模型很难拟合等问题。本文提出了一种试验环境水下声信号的特征提取方法。将水下声信号同时用梅尔频谱倒谱系数及线性预测系数处理,两者运用特征加权组合方法得到新的特征矩阵;再应用映射插值算法对特征矩阵进行处理,获得适应神经网络输入的三通道矩阵。本文选取的网络模型为残差神经网络。利用实验室所录制的对河口水库数据集测试表明,本文提出的特征提取方法普遍优于仅利用梅尔频谱倒谱系数或线性预测系数的特征处理方法。利用单频矩形脉冲信号对环境进行深度5分类,准确率平均提升2%。利用线性调频信号对环境进行深度5分类,准确率平均提升2.03%。本文提出的特征提取方法对线性调频信号在深度分类任务下处理的结果要优于单频矩形脉冲信号处理的结果。

配电网负荷预测中信号分解和预测模型组合的双层优化策略

负荷时间序列的波动性和非线性特征的加剧对负荷预测方法提出了更高的要求,而常规组合预测方法针对海量负荷数据存在应用局限性问题。为此,提出了配电网负荷预测中时序分解方法和预测模型组合的双层优化策略。首先针对某一负荷预测数据,在时序信号分解层配置权重,以负荷均方根误差最小寻优各分解方法的权重系数,进而获得各时序信号分解方法的最优组合;在此基础上,在预测模型层进行组合方案寻优,通过配置权重系数以获得各预测模型的最优组合,进一步提升负荷预测的精度。仿真结果表明,所提策略可根据预测对象的特征优化组合各信号分解方法和预测模型,降低了配电网负荷序列的非平稳性对预测精度的影响。

汽车组合仪表有意电磁干扰空时域联合分离系统设计

有意电磁波与汽车仪器电磁传输波产生的混合干扰,降低了汽车组合仪表的稳定运行能力,造成仪表设备失灵;为避免上述情况的发生,设计汽车组合仪表有意电磁干扰空时域联合分离系统;在步进电机驱动电路中,设置汽车组合仪表控制器、分离电源与PCB电路板、有意电磁干扰数字传感器;分配有意电磁波空时域源地址,通过计算干扰信号平坦度的方式,确定脉冲参数的取值范围,实现汽车组合仪表有意电磁的空时域干扰效应分析;估算有意电磁波干扰信号的数目,按照信号分解的处理原则,求解联合分离修正函数,实现电磁干扰的联合分离;在设置频率范围在100 MHz~1 GHz之间、幅度范围为0.5~5 V、脉冲宽度范围为10~100 ns区间后,通过实验结果表明,设计系统应用后可以在混合信号中,按照空域、时域标准的不同分离有意电磁波,且分离后的信号波长、信号频率依然在1.0μm、300 Hz左右,不会因混合干扰降低汽车组合仪表的稳定运行能力。

针刺联合中西药治疗肝郁肾虚型月经后期的疗效观察

目的 观察针刺联合中西药治疗肝郁肾虚型月经后期的临床疗效。方法 将88例肝郁肾虚型月经后期患者随机分为对照组(29例)、针刺组(29例)和联合组(30例)。对照组给予雌孕激素序贯疗法,针刺组在对照组基础上给予疏肝补肾针刺法,联合组在针刺组基础上给予疏肝益肾通瘀方治疗。观察3组治疗前后中医证候积分、性激素水平[雌二醇(estradial, E2)、促卵泡生成素(follicle-stimulating hormone, FSH)、促黄体生成素(luteinizing hormone, LH)水平、睾酮(testosterone, T)和催乳素(prolactin, PRL)]、卵巢功能(卵巢体积、卵泡最大直径、卵泡数量)和磷脂酰肌醇3-激酶(phosphoinositide 3-kinase, PI3K)/蛋白激酶B(protein kinase B, AKT)/哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin, mTOR)信号通路相关蛋白的变化,并比较3组临床疗效及安全性。结果 联合组总有效率为96.7%,高于对照组的72.4%(P<0.05);联合组与针刺组、针刺组与对照组比较,差异无统计学意义(P>0.05)。3组治疗后经色暗淡、腰骶酸痛、头晕耳鸣、性欲淡漠及烦躁易怒积分均低于治疗前(P<0.05);且联合组治疗后经色暗淡、腰骶酸痛、头晕耳鸣、性欲淡漠及烦躁易怒积分低于针刺组和对照组(P<0.05),针刺组低于对照组(P<0.05)。3组治疗后E2水平均高于治疗前(P<0.05),FSH、T、LH、PRL水平均低于治疗前(P<0.05);且联合组治疗后E2水平高于针刺组和对照组(P<0.05),针刺组高于对照组(P<0.05);联合组治疗后FSH、T、LH、PRL水平低于针刺组和对照组(P<0.05),针刺组低于对照组(P<0.05)。3组治疗后卵泡最大直径和卵泡数量均大于治疗前(P<0.05);且联合组治疗后卵泡最大直径和卵泡数量大于针刺组和对照组(P<0.05),针刺组大于对照组(P<0.05)。3组治疗前后卵巢体积比较,差异无统计学意义(P>0.05)。3组治疗后PI3K、Akt和m TOR蛋白表达浓度均高于治疗前(P<0.05);且联合组治疗后PI3K、Akt和mTOR蛋白表达浓度高于针刺组和对照组(P<0.05),针刺组高于对照组(P<0.05)。3组不良反应发生率比较,差异无统计学意义(P>0.05)。结论 针刺联合中西药可有效改善月经后期患者临床症状,调节性激素水平与卵巢功能,疗效显著,且安全性高,这可能与调控PI3K/Akt/m TOR信号通路有关。

中西医结合治疗高脂血症性急性胰腺炎的研究进展

高脂血症性急性胰腺炎(hyperlipidemia in acute pancreatitis,HTG-AP)是由血清甘油三酯升高引起的急性胰腺炎,临床以保守治疗为主,除西医常规治疗以外,传统中医药治疗具有显著疗效,大柴胡汤、大承气汤等化裁被临床广泛应用,且联合中药外治法治疗,效果更佳。目前,中外学者从不同靶点探究HTG-AP的治疗。文章从靶向治疗、炎症机制及肠道菌群等方面介绍HTG-AP中西医治疗的研究进展。

地奥心血康联合辛伐他汀对动脉粥样硬化小鼠的影响及机制研究

目的探讨地奥心血康(以下简称心血康)联合辛伐他汀对动脉粥样硬化(AS)小鼠的影响及机制。方法将8只C57BL/6J小鼠作为对照组,将32只ApoE^(-/-)小鼠随机分为模型组、心血康组(160 mg·kg^(-1))、辛伐他汀组(1.3 mg·kg^(-1))及联合治疗组(心血康160 mg·kg^(-1)+辛伐他汀1.3 mg·kg^(-1)),每组8只。对照组给予常规饲料喂养,其余4组均给予高脂饲料喂养。同时,各给药组均按照上述剂量灌胃给药,灌胃体积为10 mL·kg^(-1),每天1次,持续18周。给药结束后,检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平;采用油红O染色法观察小鼠主动脉斑块形成及肝脏脂质聚集情况;ELISA法检测血清前蛋白转化酶枯草溶菌素9(PCSK9)水平;qRT-PCR及Western Blot法检测肝脏组织LDLR、HNF1α、SREBP2 mRNA及蛋白表达水平。结果(1)与对照组比较,模型组小鼠的血清TC、TG、LDL-C水平显著升高(P<0.01),HDL-C水平显著降低(P<0.01);主动脉根斑块面积百分比、主动脉全体斑块面积百分比、肝脏脂滴面积百分比均显著升高(P<0.01);肝脏组织中LDLR mRNA及蛋白表达水平显著降低(P<0.01),血清PCSK9水平显著升高(P<0.01);肝脏组织中HNF1α、SREBP2 mRNA及蛋白表达水平均明显升高(P<0.05,P<0.01)。(2)与模型组比较,心血康组及联合治疗组小鼠的血清TC、TG、LDL-C水平明显降低(P<0.05,P<0.01),HDL-C水平明显升高(P<0.05);辛伐他汀组小鼠血清LDL-C水平显著降低(P<0.01);心血康组及联合治疗组小鼠的主动脉根斑块面积百分比、主动脉全体斑块面积百分比明显降低(P<0.05,P<0.01),各给药组小鼠的肝脏脂滴面积百分比均显著降低(P<0.01);心血康组及联合治疗组小鼠肝脏组织中LDLR蛋白表达水平明显升高(P<0.05),血清PCSK9水平明显降低(P<0.05,P<0.01),肝脏组织中HNF1α、SREBP2 mRNA及蛋白表达水平均明显降低(P<0.05,P<0.01)。(3)与辛伐他汀组比较,联合治疗组小鼠血清HDL-C水平明显升高(P<0.05);主动脉根斑块面积百分比、肝脏脂滴面积百分比均明显降低(P<0.05,P<0.01);肝脏组织中LDLR蛋白表达水平显著升高(P<0.01),血清PCSK9水平显著降低(P<0.01);肝脏组织中HNF1α蛋白及SREBP2 mRNA表达水平均明显降低(P<0.05,P<0.01)。结论心血康可能通过抑制SREBP2及HNF1α表达,调节PCSK9/LDLR信号途径,从而发挥对辛伐他汀降脂及抗AS作用的协同增效作用。

城市干线信号控制策略综合评估体系

城市干线信号交叉口是路网交通流汇合和分离的节点,是保障城市交通顺畅通行的关键所在。面对信号控制策略评估方法单一的问题,构建了一种面向交通效率、交通安全和交通环境的综合指标体系,并利用组合赋权-模糊综合评价(fuzzy comprehensive evaluation,FCE)模型对城市干线信号控制方案展开综合评估。最后以太原市兴华街干线的三种信控方案为例,借助VISSIM软件和间接安全模型,获取所需的评价指标值,计算综合得分。结果表明,在城市干线中使用信号联控方案最优,能有效提高干线的综合水平,其评估结果与实际结果较为一致,验证了评估体系的有效性。研究结果为城市干线信号系统的规划与管理提供了理论依据与技术支撑。

基于动态主题模型的需求弱信号识别——以比亚迪新能源汽车为例

[研究目的]竞争的加剧和创新的要求使得产品研发愈加复杂,企业急需在一个动态变化的市场中摸索前行。因此,如何有效识别需求弱信号成为亟待解决和最具挑战性的难题。[研究方法]从顾客端出发,引入并融合新颖度、出现率、支持率以及点击率等评价指标,借鉴Kim等开发的信号组合图作为信号分类体系,提出一种基于动态主题模型的需求弱信号识别方法。以“汽车之家”网站上比亚迪新能源汽车顾客评论为数据源进行实证分析,从需求弱信号识别数量和识别质量两个维度与关键词分析方法进行对比。[研究结论]结果表明,该研究提出的方法具有有效性和可行性,能够准确地识别出需求弱信号,具有更高的灵敏度。

PD-1/PD-L1抑制剂在结直肠癌治疗中的机制研究进展

免疫治疗在肿瘤治疗中占据着重要地位,已知T细胞表面的程序性死亡受体-1(programmed death receptor-1,PD-1)与肿瘤细胞上的PD-1配体1(PD-1 ligand 1,PD-L1)结合可以阻止T细胞对肿瘤细胞的免疫杀伤作用,促进肿瘤细胞的免疫逃逸。本文围绕PD-1/PD-L1抑制剂介绍了其在结直肠癌治疗中存在的问题,结直肠癌中PI3K/AKT、JAK/STAT、MAPK、NF-κB等信号通路与PD-1/PD-L1的相互作用机制,进一步探讨了PD-1/PD-L1抑制剂与肠道微生物、化疗、放疗、靶向和天然化合物的治疗研究。

不同策略提高间充质干细胞治疗肝纤维化:效果与潜在风险分析

背景:目前大量研究表明,间充质干细胞联合不同治疗策略能更有效地改善肝纤维化,抑制其向终末期肝病发展。目的:探讨间充质干细胞联合不同策略改善肝纤维化疗效优于单独间充质干细胞治疗的相关机制。方法:由第一作者应用计算机在中国知网、万方、维普、PubMed、Web of Science和Nature数据库中检索涉及间充质干细胞联合不同策略改善肝纤维化的研究,中文检索词为“间充质干细胞,肝纤维化,联合治疗,肝星状细胞”,英文检索词为“mesenchymal stem/stromal cells,liver/hepatic fibrosis/cirrhosis,combination therapy,hepatic stellate cells/HSCs”,通过快速浏览文章题目及摘要进行筛选,排除与主题关系不密切的文章,最终筛选出104篇文献进行综述分析。结果与结论:间充质干细胞通过分化为肝样细胞、抑制肝星状细胞活化、免疫调节等机制来改善肝纤维化,但间充质干细胞移植后肝脏定植率低、存活率低、作用时间短等原因限制了其临床应用。间充质干细胞联合药物、基因修饰、细胞因子等多种治疗策略改善肝纤维化的疗效优于间充质干细胞单独治疗,并且间充质干细胞联合不同策略通过促进间充质干细胞归巢、抑制肝星状细胞活化、调节微环境、调控信号通路等机制更有效地改善肝纤维化。间充质干细胞还可以通过预处理、miRNA调控及与其他细胞联合,使间充质干细胞在减轻肝纤维化方面表现出更好的肝源性分化、归巢和存活功能。间充质干细胞联合不同策略并不能规避间充质干细胞单独治疗肝纤维化的潜在风险,而且这些策略(药物、基因修饰和细胞因子等)自身安全性也值得考虑。此外,间充质干细胞移植数量和途径等有待进一步研究。