The aim of the research work is to increase the yield of cotton fiber by improving the processing technology of germinated seeds, to improve the quality indicators of seeds and lint. In order to achieve this goal, a m...The aim of the research work is to increase the yield of cotton fiber by improving the processing technology of germinated seeds, to improve the quality indicators of seeds and lint. In order to achieve this goal, a mesh surface device was created to sort the seeds into fractions. Sorting technology was developed on this device and operating modes were determined. In addition, the law of surface distribution of the fractions separated from the cotton stream moving along the surface of the net was determined, and based on the results of practical and theoretical research, a mode of sorting of cotton seeds was developed. As a result, differential equations of the law of oscillation of seeds on parallel bases, taking into account the angle of inclination for the movement of cotton seeds on the sorting surface, were constructed and programmed on a computer to construct the trajectory of seeds. On the basis of the laws of motion, the optimal value of the angle of inclination on the horizontal, which gives the state of sorting of seeds, as well as the values of the amplitude and frequency of vibrations, was determined.展开更多
The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic development.Widely adopted clinical approaches for sperm sample processing in...The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic development.Widely adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to sperm.Although a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming,none of these approaches have been fully validated by clinical-grade assessment criteria.In this study,a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is presented.Bioinspired by the temperature microenvironment in the fallopian tube during natural sperm selection,a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and fabricated.This study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45 human sperm samples.Results indicated that a temperature range of 35–36.5℃along the separation channel significantly improves human sperm motility rate((85.25±6.28)%vs.(60.72±1.37)%;P=0.0484),increases normal sperm morphology rate((16.42±1.43)%vs.(12.55±0.88)%;P<0.0001),and reduces DNA fragmentation((7.44±0.79)%vs.(10.36±0.72)%;P=0.0485)compared to the nonthermotaxis group.Sperm thermotaxis is species-specific,and selected mouse sperm displayed the highest motility in response to a temperature range of 36–37.5℃ along the separation channel.Furthermore,IVF experiments indicated that the selected sperm permitted an increased fertilization rate and improved embryonic development from zygote to blastocyst.This microfluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF success rate for patients with oligozoospermia and asthenozoospermia.展开更多
文摘The aim of the research work is to increase the yield of cotton fiber by improving the processing technology of germinated seeds, to improve the quality indicators of seeds and lint. In order to achieve this goal, a mesh surface device was created to sort the seeds into fractions. Sorting technology was developed on this device and operating modes were determined. In addition, the law of surface distribution of the fractions separated from the cotton stream moving along the surface of the net was determined, and based on the results of practical and theoretical research, a mode of sorting of cotton seeds was developed. As a result, differential equations of the law of oscillation of seeds on parallel bases, taking into account the angle of inclination for the movement of cotton seeds on the sorting surface, were constructed and programmed on a computer to construct the trajectory of seeds. On the basis of the laws of motion, the optimal value of the angle of inclination on the horizontal, which gives the state of sorting of seeds, as well as the values of the amplitude and frequency of vibrations, was determined.
基金supported by the Key Research and Development Project of Hubei Province,China(No.2021BCA111)。
文摘The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic development.Widely adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to sperm.Although a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming,none of these approaches have been fully validated by clinical-grade assessment criteria.In this study,a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is presented.Bioinspired by the temperature microenvironment in the fallopian tube during natural sperm selection,a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and fabricated.This study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45 human sperm samples.Results indicated that a temperature range of 35–36.5℃along the separation channel significantly improves human sperm motility rate((85.25±6.28)%vs.(60.72±1.37)%;P=0.0484),increases normal sperm morphology rate((16.42±1.43)%vs.(12.55±0.88)%;P<0.0001),and reduces DNA fragmentation((7.44±0.79)%vs.(10.36±0.72)%;P=0.0485)compared to the nonthermotaxis group.Sperm thermotaxis is species-specific,and selected mouse sperm displayed the highest motility in response to a temperature range of 36–37.5℃ along the separation channel.Furthermore,IVF experiments indicated that the selected sperm permitted an increased fertilization rate and improved embryonic development from zygote to blastocyst.This microfluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF success rate for patients with oligozoospermia and asthenozoospermia.
