DNA hydrogels are three-dimensional polymer networks constructed using DNA as the structural building block.Due to the tight binding between hydrophilic groups on DNA chains and water molecules,they exhibit outstandin...DNA hydrogels are three-dimensional polymer networks constructed using DNA as the structural building block.Due to the tight binding between hydrophilic groups on DNA chains and water molecules,they exhibit outstanding plasticity and fluid thermodynamic properties,making them one of the best choices for mimicking natural biological tissues.By controlling the backbone building blocks,gelation conditions,and cross-linking methods of DNA hydrogels,hydrogels with different mechanical strengths can be obtained,thus expanding their applications in the field of biology.This review first introduces the relationship between the mechanical properties of DNA hydrogels and their structure,elucidates the approaches and strategies for mechanical property modulation,and focuses on the scheme of controllable design to modulate the mechanical properties of DNA hydrogels for applications in biosensing,cellular function regulation,and bone tissue engineering.Furthermore,this review outlines the future development directions and challenges faced in the mechanical property modulation of DNA hydrogels,providing useful information for the precise design of DNA hydrogels for biological research.展开更多
DNA水凝胶是具有三维聚合物网络的高保水性材料。研究人员设计了多种DNA水凝胶交联制备方法,并通过向其中引入其他功能分子或与其他类型的功能材料相互结合,构建了具有优异性能的DNA水凝胶,受到了广泛关注。适配体是基于指数富集的配体...DNA水凝胶是具有三维聚合物网络的高保水性材料。研究人员设计了多种DNA水凝胶交联制备方法,并通过向其中引入其他功能分子或与其他类型的功能材料相互结合,构建了具有优异性能的DNA水凝胶,受到了广泛关注。适配体是基于指数富集的配体系统进化(systematic evolution of ligands by exponential enrichment,SELEX)技术从随机寡核苷酸文库中筛选获得的对目标物质具有良好特异性与亲和力的寡核苷酸序列。适配体功能化的DNA水凝胶具有靶向范围广、稳定性好、易于修饰、操作简单和成本低等优点,得到了广泛应用。本文概述了构建适配体功能化的DNA水凝胶的基本设计原则与分类,重点介绍了适配体功能化的DNA水凝胶在食品安全检测中的最新策略,最后,讨论了适配体功能化的DNA水凝胶面临的挑战以及对未来的展望,旨在为其在食品安全领域的应用提供参考。展开更多
The development of simple and accurate detection of uracil-DNA glycosylase(UDG)is of great significance for early clinical diagnosis and biomedical research.Here,we on the first effort introduced the uracil bases into...The development of simple and accurate detection of uracil-DNA glycosylase(UDG)is of great significance for early clinical diagnosis and biomedical research.Here,we on the first effort introduced the uracil bases into the rolling circle amplification(RCA)reaction to produce the functionalized pure DNA hydrogel(PDH)for UDG detection.During RCA process,methylene blue(MB)molecules as the indicators were encapsulated into PDH.The addition of UDG can remove the uracil bases of PDH to generate abasic sites,which are further cleaved with the assistance of apurinic/apyrimidinic endonuclease(APE),thus resulting in the dissociation of PDH to release blue MB.By combining with the paper analytical devices as the signal readout platform,a colorimetric and electrochemical dual-signal biosensor was constructed for convenient and accurate detection of UDG.The proposed MB@PDH-based dual-signal sensing system exhibited good selectivity and high sensitivity with a detection limit of 6.4104 U/mL(electrochemical method).It was also demonstrated that this sensing system showed excellent performance in UDG inhibitor screening,thus providing great potential in UDG-related disease diagnosis and drug discovery.展开更多
Segmental bone defects,stemming from trauma,infection,and tumors,pose formidable clinical challenges.Traditional bone repair materials,such as autologous and allogeneic bone grafts,grapple with limitations including s...Segmental bone defects,stemming from trauma,infection,and tumors,pose formidable clinical challenges.Traditional bone repair materials,such as autologous and allogeneic bone grafts,grapple with limitations including source scarcity and immune rejection risks.The advent of nucleic acid nanotechnology,particularly the use of DNA hydrogels in tissue engineering,presents a promising solution,attributed to their biocompatibility,biodegradability,and programmability.However,these hydrogels,typically hindered by high gelation temperatures(~46◦C)and high construction costs,limit cell encapsulation and broader application.Our research introduces a novel polymer-modified DNA hydrogel,developed using nucleic acid nanotechnology,which gels at a more biocompatible temperature of 37◦C and is cost-effective.This hydrogel then incorporates tetrahedral Framework Nucleic Acid(tFNA)to enhance osteogenic mineralization.Furthermore,considering the modifiability of tFNA,we modified its chains with Aptamer02(Apt02),an aptamer known to foster angiogenesis.This dual approach significantly accelerates osteogenic differentiation in bone marrow stromal cells(BMSCs)and angiogenesis in human umbilical vein endothelial cells(HUVECs),with cell sequencing confirming their targeting efficacy,respectively.In vivo experiments in rats with critical-size cranial bone defects demonstrate their effectiveness in enhancing new bone formation.This innovation not only offers a viable solution for repairing segmental bone defects but also opens avenues for future advancements in bone organoids construction,marking a significant advancement in tissue engineering and regenerative medicine.展开更多
基金supported by the National Key Research and Development Program of China(2023YFB3208204)the National Natural Science Foundation of China(12305400,12105352)+2 种基金the Natural Science Foundation of Shanghai,China(22ZR1470600)the Natural Science Foundation of Shandong Province(ZR2019MB068,ZR2022MB012,ZR2021QE167)the Xiangfu Lab Research Project(XF012022E0100).
文摘DNA hydrogels are three-dimensional polymer networks constructed using DNA as the structural building block.Due to the tight binding between hydrophilic groups on DNA chains and water molecules,they exhibit outstanding plasticity and fluid thermodynamic properties,making them one of the best choices for mimicking natural biological tissues.By controlling the backbone building blocks,gelation conditions,and cross-linking methods of DNA hydrogels,hydrogels with different mechanical strengths can be obtained,thus expanding their applications in the field of biology.This review first introduces the relationship between the mechanical properties of DNA hydrogels and their structure,elucidates the approaches and strategies for mechanical property modulation,and focuses on the scheme of controllable design to modulate the mechanical properties of DNA hydrogels for applications in biosensing,cellular function regulation,and bone tissue engineering.Furthermore,this review outlines the future development directions and challenges faced in the mechanical property modulation of DNA hydrogels,providing useful information for the precise design of DNA hydrogels for biological research.
文摘DNA水凝胶是具有三维聚合物网络的高保水性材料。研究人员设计了多种DNA水凝胶交联制备方法,并通过向其中引入其他功能分子或与其他类型的功能材料相互结合,构建了具有优异性能的DNA水凝胶,受到了广泛关注。适配体是基于指数富集的配体系统进化(systematic evolution of ligands by exponential enrichment,SELEX)技术从随机寡核苷酸文库中筛选获得的对目标物质具有良好特异性与亲和力的寡核苷酸序列。适配体功能化的DNA水凝胶具有靶向范围广、稳定性好、易于修饰、操作简单和成本低等优点,得到了广泛应用。本文概述了构建适配体功能化的DNA水凝胶的基本设计原则与分类,重点介绍了适配体功能化的DNA水凝胶在食品安全检测中的最新策略,最后,讨论了适配体功能化的DNA水凝胶面临的挑战以及对未来的展望,旨在为其在食品安全领域的应用提供参考。
基金supported by the National Natural Science Foundation of China(NSFC,Grant No.21922601).
文摘The development of simple and accurate detection of uracil-DNA glycosylase(UDG)is of great significance for early clinical diagnosis and biomedical research.Here,we on the first effort introduced the uracil bases into the rolling circle amplification(RCA)reaction to produce the functionalized pure DNA hydrogel(PDH)for UDG detection.During RCA process,methylene blue(MB)molecules as the indicators were encapsulated into PDH.The addition of UDG can remove the uracil bases of PDH to generate abasic sites,which are further cleaved with the assistance of apurinic/apyrimidinic endonuclease(APE),thus resulting in the dissociation of PDH to release blue MB.By combining with the paper analytical devices as the signal readout platform,a colorimetric and electrochemical dual-signal biosensor was constructed for convenient and accurate detection of UDG.The proposed MB@PDH-based dual-signal sensing system exhibited good selectivity and high sensitivity with a detection limit of 6.4104 U/mL(electrochemical method).It was also demonstrated that this sensing system showed excellent performance in UDG inhibitor screening,thus providing great potential in UDG-related disease diagnosis and drug discovery.
基金supported by National Natural Science Foundation of China(82230071,82172098)Integrated Project of Major Research Plan of National Natural Science Foundation of China(92249303)+1 种基金Shanghai Committee of Science and Technology(23141900600,Laboratory Animal Research Project)Shanghai Clinical Research Plan of SHDC2023CRT01.
文摘Segmental bone defects,stemming from trauma,infection,and tumors,pose formidable clinical challenges.Traditional bone repair materials,such as autologous and allogeneic bone grafts,grapple with limitations including source scarcity and immune rejection risks.The advent of nucleic acid nanotechnology,particularly the use of DNA hydrogels in tissue engineering,presents a promising solution,attributed to their biocompatibility,biodegradability,and programmability.However,these hydrogels,typically hindered by high gelation temperatures(~46◦C)and high construction costs,limit cell encapsulation and broader application.Our research introduces a novel polymer-modified DNA hydrogel,developed using nucleic acid nanotechnology,which gels at a more biocompatible temperature of 37◦C and is cost-effective.This hydrogel then incorporates tetrahedral Framework Nucleic Acid(tFNA)to enhance osteogenic mineralization.Furthermore,considering the modifiability of tFNA,we modified its chains with Aptamer02(Apt02),an aptamer known to foster angiogenesis.This dual approach significantly accelerates osteogenic differentiation in bone marrow stromal cells(BMSCs)and angiogenesis in human umbilical vein endothelial cells(HUVECs),with cell sequencing confirming their targeting efficacy,respectively.In vivo experiments in rats with critical-size cranial bone defects demonstrate their effectiveness in enhancing new bone formation.This innovation not only offers a viable solution for repairing segmental bone defects but also opens avenues for future advancements in bone organoids construction,marking a significant advancement in tissue engineering and regenerative medicine.
