High selective molecularly imprinted polymers （MIPs） for tetracycline have been prepared by precipitation polymerization. Effects of monomer and solvent, the ratio of monomer and template and the characterization of...High selective molecularly imprinted polymers （MIPs） for tetracycline have been prepared by precipitation polymerization. Effects of monomer and solvent, the ratio of monomer and template and the characterization of the polymer were investigated by frontal chromatography and selectivity experiment. The results clearly indicated that the polymer, which had the highest molecular recognition abilities for tetracycline antibiotics, had been received.展开更多
（S）-4-Phenyl-2-oxazolidinone imprinted polymers were prepared by using methacrylic acid （MAA） as the functional monomer, and divinylbenzene （DVB） as crosslinker. The factors, which influence the selectivity of t...（S）-4-Phenyl-2-oxazolidinone imprinted polymers were prepared by using methacrylic acid （MAA） as the functional monomer, and divinylbenzene （DVB） as crosslinker. The factors, which influence the selectivity of the polymers were explored. Effective separation was observed for racemic 4-phenyl-2-oxazolidinone in mobile phase of acetonitrile. The investigation of mobile phase suggested that the hydrogen bonds between template and functional monomer was a primary factor in chiral recognition, while the preparation of polymers implied that the л-л stacking interaction between template and crosslinker played a role in imprinting procedure.展开更多
Molecularly imprinted polymers (MIPs) have great potential as adsorbents for selective adsorption and separation of nucleoside compounds,but effectively enhancing the affinity of recognition sites by adjusting the for...Molecularly imprinted polymers (MIPs) have great potential as adsorbents for selective adsorption and separation of nucleoside compounds,but effectively enhancing the affinity of recognition sites by adjusting the forces between template molecules and functional monomers remains an important challenge.In this work,a surface imprinting strategy was used to construct bowl-shaped molecularly imprinted composite sorbents (BHPN@MIPs) based on polydopamine (PDA) particles and have achieved selective separation and purification of 2'-deoxyadenosine (dA).Where by the base complementary pairing interaction of the combined template molecule d A and the pyrimidine functional monomer can enhance the preassembly force,and the hydrophilic bowl-shaped PDA can provide a larger storage space contact efficiency of d A in the test solution,causing the site utilization much higher and improving the kinetic adsorption performance.The equilibrium adsorption time and maximum adsorption capacity of60 min and 328.45μmol·g^(-1)were observed by static adsorption experiments,and the selectivity experimental results showed an imprinting factor IF of 1.30.After four adsorption–desorption cycles,the initial adsorption equilibrium adsorption capacity of BHPN@MIPs still retained 91.14%.By evaluating the selective adsorption of d A in spiked human serum solutions,BHPN@MIPs can be used to selectively enrich and analyze target d A in complex biological samples.展开更多
In this work the enhanced molecularly imprinted optosensing material based on graphene oxide-quantum dots （ GO- QDs） was synthesized for highly selective and sensitive specific recognition of the target protein, bov...In this work the enhanced molecularly imprinted optosensing material based on graphene oxide-quantum dots （ GO- QDs） was synthesized for highly selective and sensitive specific recognition of the target protein, bovine serum albumin （BSA）. Here, GO was introduced to enhance the efficiency of mass-transfer in recognition of target protein. Molecularly imprinted polymer coated GO-QDs using BSA as template （BMIP-coated GO-QDs ） exhibited a fast mass-transfer speed, which could be ascribed to the high volume of efficient surface area and high target recognition efficiency of the synthesized nanoscale device. Under optimal conditions, it was found that the BSA as target protein could remarkably quench the relative fluorescence intensity of BMIP- coated GO-QDs linearly in a concentration-dependent manner that was best described by a Stern-Volmer equation. The Ksv （Stern- Volmer constant） for template BSA was much higher than bovine hemoglobin （BHb） and lysozyme （Lyz）, implying a highly selective recognition ability of the BMIP-coated GO-QDs to BSA. This enhanced fluorescent nanoscale device may provide opportunities to develop a system that is efficient and effective and has potential in the design of highly effective fluorescent receptor for recognition of target protein in Droteomics studies.展开更多
Molecularly imprinted polymers selective for L-aspartic acid (LAA) have been prepared using the carboxy-betaine polymer bearing zwitterionic centres along the backbone. LAA is well known to promote good me-tabolism, t...Molecularly imprinted polymers selective for L-aspartic acid (LAA) have been prepared using the carboxy-betaine polymer bearing zwitterionic centres along the backbone. LAA is well known to promote good me-tabolism, treat fatigue and depression along with its significance in accurate age estimation in the field of forensic science and is an important constituent of ‘aspartame’, the low calorie sweetener. In order to study the intermolecular interactions in the prepolymerization mixture between the monomer and the template (LAA)/non-template (DAA), a computational approach was developed. It was based on the binding energy of the complex between the template and functional monomer. The results demonstrate that electrostatic in-teractions primarily guide the imprinting protocol. The MIP was able to selectively and specifically take up LAA from aqueous solution, human blood serum and certain pharmaceutical samples quantitatively. Hence, a facile, specific and selective technique to detect the amino acid, LAA in the presence of various interfer-rants, in different kinds of matrices is presented.展开更多
Sulfamethoxazole(SMX)is a sulfonamide antibiotic primarily used to treat urinary tract infections and used in veterinary and industrialized husbandry to treat diseases and food additives.Like other antibiotics,SMX is ...Sulfamethoxazole(SMX)is a sulfonamide antibiotic primarily used to treat urinary tract infections and used in veterinary and industrialized husbandry to treat diseases and food additives.Like other antibiotics,SMX is considered as a pollutant in water and food that threaten local life.This study developed a surface plasmon resonance(SPR)sensor chip that is fast,highly selective,and reusable,and requires no pretreatment for detecting SMX.As a receptor,SMX imprinted methacrylic acid-2-hydroxyethyl methacrylate-ethylene glycol dimethacrylate polymer[poly(MAA-HEMA-EGDMA)]was used.The surface of the gold SPR chips was coated with a drop-casting method.The nanofilm coated chips were characterized by scanning electron microscopy(SEM),atomic force microscopy(AFM),ellipsometer,contact angle measurement,and Fourier-transform infrared spectrometry(FTIR).Imprinting factor(IF)was calculated as:ΔR[MIP(molecularly imprinted polymers)]/ΔR[NIP(non-imprinted)]=12/3.5=3.4.Limit of detection(LOD)and limit of quantification(LOQ)values were calculated with 3 s/m and 10 s/m methods,and the results were found to be 0.0011μg/L for LOD 0.0034μg/L for LOQ.Adsorption studies on both standard SMX solution and commercial milk samples were applied.Also,we investigated the developed chip’s reusability,storability,and selectivity with amoxicillin and cefalexin.展开更多
A pre-treatment methodology for clenbuterol hydrochloride （CLEN） isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers （MIPs）. CLEN-imprinted pol-...A pre-treatment methodology for clenbuterol hydrochloride （CLEN） isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers （MIPs）. CLEN-imprinted pol- ymers were synthesized by the combined use of ally-β-cyclodextrin （ally-13-CD） and methacrylic acid （MAA）, allyl-β-CD and acrylonitrile （AN）, and allyl-β-CD and methyl methacrylate （MMA） as the binary functional monomers. MAA-linked allyl-β-CD MIPs （M-MAA） were characterized by Fourier transform-infrared （FT-IR） spectroscopy and a scanning electron microscope （SEM）. Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references： AN-linked allyl-β-CD MIPs （M-AN） and MMA-linked allyl-β-CD MIPs （M-MMA）. M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction （MISPE） micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation （RSD） ≤4.45%.展开更多
Perfluorooctane sulfonate(PFOS),as a potential persistent organic pollutant,has been widely detected in water environments,and has become a great concern in recent years.PFOS is very stable and difficult to decompose ...Perfluorooctane sulfonate(PFOS),as a potential persistent organic pollutant,has been widely detected in water environments,and has become a great concern in recent years.PFOS is very stable and difficult to decompose using conventional techniques.Sorption may be an attractive method to remove it from water.In this study,the molecularly imprinted polymer(MIP)adsorbents were prepared through the polymerization of 4-vinylpyridine under different preparation conditions in order to remove perfluorooctane sulfonate(PFOS)from water.The MIP adsorbents using perfluorooctanoic acid(PFOA)as the template had good imprinting effects and could selectively remove PFOS from aqueous solution.The sorption behaviors including sorption kinetics,isotherms,and effect of pH,salt,and competitive anions were investigated.Experimental results showed that the sorption of PFOS on the MIP adsorbents was very fast,pHdependent,and highly selective.The achieved fast sorption equilibrium within 1 h was attributed to the surface sorption on the fine adsorbents.The sorption isotherms showed that the sorption selectivity of PFOS on the MIP adsorbents decreased at high PFOS concentrations,which may be due to the double-layer sorption and the formation of PFOS micelles on the sorbent surface.The sorption of PFOS on the MIP adsorbents was mainly dominated by the electrostatic interaction between the protonated vinylpyridine on the adsorbent surface and the anionic PFOS.The prepared MIP adsorbents can potentially be applied in water and wastewater treatment for selective removal of PFOS.展开更多
Two molecularly imprinted polymers binding to analgesic acetanilide were prepared using either dual functional monomers of calix[4]arene derivative and acrylamide or single monomer acrylamide, respectively. The polyme...Two molecularly imprinted polymers binding to analgesic acetanilide were prepared using either dual functional monomers of calix[4]arene derivative and acrylamide or single monomer acrylamide, respectively. The polymers were ground, sieved and investigated by equilibrium binding experiment to evaluate their recognition properties for the template and other substrates. Scatchard analysis showed that homogeneous recognition sites were formed in the imprinted polymer matrix. Our results demonstrated that the polymer using two functional monomers exhibited better selectivity for the template. This study may open new frontiers for the development and application of imprinted polymers, such as drug separation and purification.展开更多
文摘High selective molecularly imprinted polymers （MIPs） for tetracycline have been prepared by precipitation polymerization. Effects of monomer and solvent, the ratio of monomer and template and the characterization of the polymer were investigated by frontal chromatography and selectivity experiment. The results clearly indicated that the polymer, which had the highest molecular recognition abilities for tetracycline antibiotics, had been received.
基金financed by the National Natural Science Foundation of China(No.20202015).
文摘（S）-4-Phenyl-2-oxazolidinone imprinted polymers were prepared by using methacrylic acid （MAA） as the functional monomer, and divinylbenzene （DVB） as crosslinker. The factors, which influence the selectivity of the polymers were explored. Effective separation was observed for racemic 4-phenyl-2-oxazolidinone in mobile phase of acetonitrile. The investigation of mobile phase suggested that the hydrogen bonds between template and functional monomer was a primary factor in chiral recognition, while the preparation of polymers implied that the л-л stacking interaction between template and crosslinker played a role in imprinting procedure.
基金financially supported by the National Natural Science Foundation of China (22078132 and 22108103)Open Funding Project of the National Key Laboratory of Biochemical Engineering (2021KF-02)+3 种基金China Postdoctoral Science Foundation (2021M691301)Jiangsu Agricultural Independent Innovation Fund Project (CX(21)3079)Graduate Research Innovation Program of Jiangsu Province (KYCX20-3040)China Postdoctoral Science Foundation (2021M691301)。
文摘Molecularly imprinted polymers (MIPs) have great potential as adsorbents for selective adsorption and separation of nucleoside compounds,but effectively enhancing the affinity of recognition sites by adjusting the forces between template molecules and functional monomers remains an important challenge.In this work,a surface imprinting strategy was used to construct bowl-shaped molecularly imprinted composite sorbents (BHPN@MIPs) based on polydopamine (PDA) particles and have achieved selective separation and purification of 2'-deoxyadenosine (dA).Where by the base complementary pairing interaction of the combined template molecule d A and the pyrimidine functional monomer can enhance the preassembly force,and the hydrophilic bowl-shaped PDA can provide a larger storage space contact efficiency of d A in the test solution,causing the site utilization much higher and improving the kinetic adsorption performance.The equilibrium adsorption time and maximum adsorption capacity of60 min and 328.45μmol·g^(-1)were observed by static adsorption experiments,and the selectivity experimental results showed an imprinting factor IF of 1.30.After four adsorption–desorption cycles,the initial adsorption equilibrium adsorption capacity of BHPN@MIPs still retained 91.14%.By evaluating the selective adsorption of d A in spiked human serum solutions,BHPN@MIPs can be used to selectively enrich and analyze target d A in complex biological samples.
文摘In this work the enhanced molecularly imprinted optosensing material based on graphene oxide-quantum dots （ GO- QDs） was synthesized for highly selective and sensitive specific recognition of the target protein, bovine serum albumin （BSA）. Here, GO was introduced to enhance the efficiency of mass-transfer in recognition of target protein. Molecularly imprinted polymer coated GO-QDs using BSA as template （BMIP-coated GO-QDs ） exhibited a fast mass-transfer speed, which could be ascribed to the high volume of efficient surface area and high target recognition efficiency of the synthesized nanoscale device. Under optimal conditions, it was found that the BSA as target protein could remarkably quench the relative fluorescence intensity of BMIP- coated GO-QDs linearly in a concentration-dependent manner that was best described by a Stern-Volmer equation. The Ksv （Stern- Volmer constant） for template BSA was much higher than bovine hemoglobin （BHb） and lysozyme （Lyz）, implying a highly selective recognition ability of the BMIP-coated GO-QDs to BSA. This enhanced fluorescent nanoscale device may provide opportunities to develop a system that is efficient and effective and has potential in the design of highly effective fluorescent receptor for recognition of target protein in Droteomics studies.
文摘Molecularly imprinted polymers selective for L-aspartic acid (LAA) have been prepared using the carboxy-betaine polymer bearing zwitterionic centres along the backbone. LAA is well known to promote good me-tabolism, treat fatigue and depression along with its significance in accurate age estimation in the field of forensic science and is an important constituent of ‘aspartame’, the low calorie sweetener. In order to study the intermolecular interactions in the prepolymerization mixture between the monomer and the template (LAA)/non-template (DAA), a computational approach was developed. It was based on the binding energy of the complex between the template and functional monomer. The results demonstrate that electrostatic in-teractions primarily guide the imprinting protocol. The MIP was able to selectively and specifically take up LAA from aqueous solution, human blood serum and certain pharmaceutical samples quantitatively. Hence, a facile, specific and selective technique to detect the amino acid, LAA in the presence of various interfer-rants, in different kinds of matrices is presented.
文摘Sulfamethoxazole(SMX)is a sulfonamide antibiotic primarily used to treat urinary tract infections and used in veterinary and industrialized husbandry to treat diseases and food additives.Like other antibiotics,SMX is considered as a pollutant in water and food that threaten local life.This study developed a surface plasmon resonance(SPR)sensor chip that is fast,highly selective,and reusable,and requires no pretreatment for detecting SMX.As a receptor,SMX imprinted methacrylic acid-2-hydroxyethyl methacrylate-ethylene glycol dimethacrylate polymer[poly(MAA-HEMA-EGDMA)]was used.The surface of the gold SPR chips was coated with a drop-casting method.The nanofilm coated chips were characterized by scanning electron microscopy(SEM),atomic force microscopy(AFM),ellipsometer,contact angle measurement,and Fourier-transform infrared spectrometry(FTIR).Imprinting factor(IF)was calculated as:ΔR[MIP(molecularly imprinted polymers)]/ΔR[NIP(non-imprinted)]=12/3.5=3.4.Limit of detection(LOD)and limit of quantification(LOQ)values were calculated with 3 s/m and 10 s/m methods,and the results were found to be 0.0011μg/L for LOD 0.0034μg/L for LOQ.Adsorption studies on both standard SMX solution and commercial milk samples were applied.Also,we investigated the developed chip’s reusability,storability,and selectivity with amoxicillin and cefalexin.
基金Project supported by the Department of Science and Technology of Zhejiang Province(No.2013C02022-2/01),China
文摘A pre-treatment methodology for clenbuterol hydrochloride （CLEN） isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers （MIPs）. CLEN-imprinted pol- ymers were synthesized by the combined use of ally-β-cyclodextrin （ally-13-CD） and methacrylic acid （MAA）, allyl-β-CD and acrylonitrile （AN）, and allyl-β-CD and methyl methacrylate （MMA） as the binary functional monomers. MAA-linked allyl-β-CD MIPs （M-MAA） were characterized by Fourier transform-infrared （FT-IR） spectroscopy and a scanning electron microscope （SEM）. Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references： AN-linked allyl-β-CD MIPs （M-AN） and MMA-linked allyl-β-CD MIPs （M-MMA）. M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction （MISPE） micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation （RSD） ≤4.45%.
基金This work was supported by the National Natural Science Foundation of China(Grant No.50608045)the special fund of State Key Joint Laboratory of Environment Simulation and Pollution(Grant No.08Z04ESPCT)the National Outstanding Youth Foundation of China(Grant No.50625823).
文摘Perfluorooctane sulfonate(PFOS),as a potential persistent organic pollutant,has been widely detected in water environments,and has become a great concern in recent years.PFOS is very stable and difficult to decompose using conventional techniques.Sorption may be an attractive method to remove it from water.In this study,the molecularly imprinted polymer(MIP)adsorbents were prepared through the polymerization of 4-vinylpyridine under different preparation conditions in order to remove perfluorooctane sulfonate(PFOS)from water.The MIP adsorbents using perfluorooctanoic acid(PFOA)as the template had good imprinting effects and could selectively remove PFOS from aqueous solution.The sorption behaviors including sorption kinetics,isotherms,and effect of pH,salt,and competitive anions were investigated.Experimental results showed that the sorption of PFOS on the MIP adsorbents was very fast,pHdependent,and highly selective.The achieved fast sorption equilibrium within 1 h was attributed to the surface sorption on the fine adsorbents.The sorption isotherms showed that the sorption selectivity of PFOS on the MIP adsorbents decreased at high PFOS concentrations,which may be due to the double-layer sorption and the formation of PFOS micelles on the sorbent surface.The sorption of PFOS on the MIP adsorbents was mainly dominated by the electrostatic interaction between the protonated vinylpyridine on the adsorbent surface and the anionic PFOS.The prepared MIP adsorbents can potentially be applied in water and wastewater treatment for selective removal of PFOS.
基金Project supported by the National Natural Science Foundation of China (Nos. 20175009 and 20375017) the Doctoral Foundation of Education Ministry of China (No. 2002055002) and Tianjin Natural Science Foundation (No. 033603511).
文摘Two molecularly imprinted polymers binding to analgesic acetanilide were prepared using either dual functional monomers of calix[4]arene derivative and acrylamide or single monomer acrylamide, respectively. The polymers were ground, sieved and investigated by equilibrium binding experiment to evaluate their recognition properties for the template and other substrates. Scatchard analysis showed that homogeneous recognition sites were formed in the imprinted polymer matrix. Our results demonstrated that the polymer using two functional monomers exhibited better selectivity for the template. This study may open new frontiers for the development and application of imprinted polymers, such as drug separation and purification.
