Herein,a method of true-temperature inversion for a multi-wavelength pyrometer based on fractional-order particle-swarm optimization is proposed for difficult inversion problems with unknown emissivity.Fractional-order...Herein,a method of true-temperature inversion for a multi-wavelength pyrometer based on fractional-order particle-swarm optimization is proposed for difficult inversion problems with unknown emissivity.Fractional-order calculus has the inherent advantage of easily jumping out of local extreme values;here,it is introduced into the particle-swarm algorithm to invert the true temperature.An improved adaptive-adjustment mechanism is applied to automatically adjust the current velocity order of the particles and update their velocity and position values,increasing the accuracy of the true temperature values.The results of simulations using the proposed algorithm were compared with three algorithms using typical emissivity models:the internal penalty function algorithm,the optimization function(fmincon)algorithm,and the conventional particle-swarm optimization algorithm.The results show that the proposed algorithm has good accuracy for true-temperature inversion.Actual experimental results from a rocket-motor plume were used to demonstrate that the true-temperature inversion results of this algorithm are in good agreement with the theoretical true-temperature values.展开更多
Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously anal...Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample,such as different brain areas.In addition,conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm,a numerical aperture of 0.5,and a working wavelength range from 400 to 1000 nm.We achieved a resolution of 0.74μm in fluorescent beads imaging.The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system,a confocal laser scanning system,and a two-photon imaging system.This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.展开更多
[Objectives]To establish a HPLC characteristic spectrum of honeysuckle stem formula granules,and to evaluate the quality consistency based on the standard decoction.[Methods]Using Agilent ZORBAX SB-C_(18)(5μm,250 mm&...[Objectives]To establish a HPLC characteristic spectrum of honeysuckle stem formula granules,and to evaluate the quality consistency based on the standard decoction.[Methods]Using Agilent ZORBAX SB-C_(18)(5μm,250 mm×4.6 mm)chromatographic column;gradient elution with acetonitrile-0.4%phosphoric acid solution(0.2:99.8,V/V),column temperature at 25℃;detection wavelength at 236 nm;flow rate 1.0 mL/min;using"Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine",to evaluate the similarity of the characteristic chromatogram of crude drug,standard decoction and formula granules of honeysuckle stem.[Results]The similarity of characteristic chromatogram was higher than 0.94,and 6 components were identified from 10 characteristic peaks.[Conclusions]The established method can more comprehensively reflect the overall appearance of the chemical substances in the honeysuckle stem and the transmission law,and can provide an experimental basis for the consistency of the quality of the honeysuckle stem formula granules.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.62205280)the Graduate Innovation Foundation of Yantai University(Grant No.GGIFYTU2348).
文摘Herein,a method of true-temperature inversion for a multi-wavelength pyrometer based on fractional-order particle-swarm optimization is proposed for difficult inversion problems with unknown emissivity.Fractional-order calculus has the inherent advantage of easily jumping out of local extreme values;here,it is introduced into the particle-swarm algorithm to invert the true temperature.An improved adaptive-adjustment mechanism is applied to automatically adjust the current velocity order of the particles and update their velocity and position values,increasing the accuracy of the true temperature values.The results of simulations using the proposed algorithm were compared with three algorithms using typical emissivity models:the internal penalty function algorithm,the optimization function(fmincon)algorithm,and the conventional particle-swarm optimization algorithm.The results show that the proposed algorithm has good accuracy for true-temperature inversion.Actual experimental results from a rocket-motor plume were used to demonstrate that the true-temperature inversion results of this algorithm are in good agreement with the theoretical true-temperature values.
基金supported by National Key R&D Program of China(grant no.2022YFC2404201)the Chinese Academy of Sciences Project for Young Scientists in Basic Research(grant no.YSBR067).
文摘Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample,such as different brain areas.In addition,conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm,a numerical aperture of 0.5,and a working wavelength range from 400 to 1000 nm.We achieved a resolution of 0.74μm in fluorescent beads imaging.The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system,a confocal laser scanning system,and a two-photon imaging system.This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.
基金Supported by Major Project of Nanning Scientific Research and Technology Development Plan in 2020(20201048)。
文摘[Objectives]To establish a HPLC characteristic spectrum of honeysuckle stem formula granules,and to evaluate the quality consistency based on the standard decoction.[Methods]Using Agilent ZORBAX SB-C_(18)(5μm,250 mm×4.6 mm)chromatographic column;gradient elution with acetonitrile-0.4%phosphoric acid solution(0.2:99.8,V/V),column temperature at 25℃;detection wavelength at 236 nm;flow rate 1.0 mL/min;using"Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine",to evaluate the similarity of the characteristic chromatogram of crude drug,standard decoction and formula granules of honeysuckle stem.[Results]The similarity of characteristic chromatogram was higher than 0.94,and 6 components were identified from 10 characteristic peaks.[Conclusions]The established method can more comprehensively reflect the overall appearance of the chemical substances in the honeysuckle stem and the transmission law,and can provide an experimental basis for the consistency of the quality of the honeysuckle stem formula granules.
文摘目的建立远志薄层色谱(thin-layer chromatography,TLC)及超高效液相色谱(ultra-high performance liquid chromatography,UHPLC)特征图谱,并分析远志全根、根皮和木心间化学成分差异,为远志质量评价提供参考。方法以3,6’-二芥子酰基蔗糖、细叶远志苷A和远志皂苷B为指标成分建立TLC图谱;用UHPLC法建立远志特征图谱,结合热图聚类分析、主成分分析(principal component analysis,PCA)和正交偏最小二乘法判别分析(partial least squares discriminant analysis,OPLS-DA)评价多批远志全根、根皮和木心之间的差异性。结果TLC图谱斑点清晰、分离度较好;UHPLC特征图谱共标定28个共有峰,指认了其中的7个特征峰,13批次远志特征图谱与对照图谱相似度均大于0.9,批次间质量较为稳定;TLC和UHPLC特征图谱显示远志全根、根皮和木心化学组成一致,未发现特异成分,热图聚类分析和PCA显示远志全根与根皮中各成分峰面积接近,OPLSDA筛选出10个变量投影重要性(variable important in projection,VIP)值大于1的差异性成分。结论所建立的TLC及UHPLC特征图谱简便、准确、稳定,展开系统和提取溶剂均使用低毒试剂,符合绿色化学理念,适用于远志的质量评价,从化学分析角度说明木心的存在对远志整体化学成分影响较小。
