A multianalyte biosensor for the simultaneous determination of glucose and galactose was developed by immobilizing glucose oxidase (GOD) and galactose oxidase (GAO) on Nafion-modified thin film platinum disk electrod...A multianalyte biosensor for the simultaneous determination of glucose and galactose was developed by immobilizing glucose oxidase (GOD) and galactose oxidase (GAO) on Nafion-modified thin film platinum disk electrodes. The dual Pt working electrodes with disk shape and the surrounding ring shaped counter electrode were fabricated by thin film technology, which were integrated onto the same microchip. The response of the designed biosensor for glucose and galactose were linear up to 6.0 mmol/L and 3.5 mmol/L with sensitivities of 0.3 mA/mmol/L and 0.12 mA/mmol/L, respectively. No cross-talking effect was observed.展开更多
An amperometric multianalyte biosensor for the simultaneous determination of glucose and galactose was de-veloped based on chamber-type electrodes, which were fabricated by micromachining technology. The dual cham-ber...An amperometric multianalyte biosensor for the simultaneous determination of glucose and galactose was de-veloped based on chamber-type electrodes, which were fabricated by micromachining technology. The dual cham-ber-type enzyme electrode with glucose and galactose sensor elements was integrated onto one microchip. The ex-perimental parameters of this biosensor were optimized. The biosensor exhibited a linearity of up to 4.0 mol/L for glucose and 4.5 mol/L for galactose, and the response time was about 30 s for glucose and 40 s for galactose. No cross-talking behavior was investigated in the course of simultaneous measurement of the two analytes. Interference from electroactive species, such as ascorbic acid and uric acid, was minimized due to the permselectivity of Nafion film. In addition, the biosensor displayed a storage stability of longer than one month.展开更多
文摘A multianalyte biosensor for the simultaneous determination of glucose and galactose was developed by immobilizing glucose oxidase (GOD) and galactose oxidase (GAO) on Nafion-modified thin film platinum disk electrodes. The dual Pt working electrodes with disk shape and the surrounding ring shaped counter electrode were fabricated by thin film technology, which were integrated onto the same microchip. The response of the designed biosensor for glucose and galactose were linear up to 6.0 mmol/L and 3.5 mmol/L with sensitivities of 0.3 mA/mmol/L and 0.12 mA/mmol/L, respectively. No cross-talking effect was observed.
基金Shanghai Rising Star Project of Shanghai Science and Technology Committee (No. 04QMX1443) the Science Foundation of Shanghai Education Commission and Applied Chemistry Key Laboratory Foundation of Shanghai Normal University.
文摘An amperometric multianalyte biosensor for the simultaneous determination of glucose and galactose was de-veloped based on chamber-type electrodes, which were fabricated by micromachining technology. The dual cham-ber-type enzyme electrode with glucose and galactose sensor elements was integrated onto one microchip. The ex-perimental parameters of this biosensor were optimized. The biosensor exhibited a linearity of up to 4.0 mol/L for glucose and 4.5 mol/L for galactose, and the response time was about 30 s for glucose and 40 s for galactose. No cross-talking behavior was investigated in the course of simultaneous measurement of the two analytes. Interference from electroactive species, such as ascorbic acid and uric acid, was minimized due to the permselectivity of Nafion film. In addition, the biosensor displayed a storage stability of longer than one month.
