Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermed...Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed.展开更多
The implementation of multiple enzymes for chemical production in a cell‐free scenario is an emerging field in biomanufacturing.It enables the redesign and reconstitution of new enzymatic routes for producing chemica...The implementation of multiple enzymes for chemical production in a cell‐free scenario is an emerging field in biomanufacturing.It enables the redesign and reconstitution of new enzymatic routes for producing chemicals that may be hard to obtain from natural pathways.Although the construction of a cell‐free multienzyme system is highly flexible and adaptable,it is challenging to make all enzymatic reactions act in concert.Recently,modular construction has been conceptual‐ized as an effective way to harmonize diverse enzymatic reactions.In this review,we introduce the concept of a multienzyme module and exemplify representative modules found in Nature.We then categorize recent developments of synthetic multienzyme modules into main‐reaction modules and auxiliary modules according to their roles in reaction routes.We highlight four main‐reaction mod‐ules that can perform carbon metabolism,carbon assimilation,protein glycosylation and nonribo‐somal peptide synthesis,and exemplify auxiliary modules used for energy supply,protection and reinforcement for main reactions.The reactor‐level modularization of multienzyme catalysis is also discussed.展开更多
基金supported by the National Natural Science Foundation of China(21878324,21706273)the CAS Pioneer Hundred Talent Program(Type C,reference#2016-081)。
文摘Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed.
文摘The implementation of multiple enzymes for chemical production in a cell‐free scenario is an emerging field in biomanufacturing.It enables the redesign and reconstitution of new enzymatic routes for producing chemicals that may be hard to obtain from natural pathways.Although the construction of a cell‐free multienzyme system is highly flexible and adaptable,it is challenging to make all enzymatic reactions act in concert.Recently,modular construction has been conceptual‐ized as an effective way to harmonize diverse enzymatic reactions.In this review,we introduce the concept of a multienzyme module and exemplify representative modules found in Nature.We then categorize recent developments of synthetic multienzyme modules into main‐reaction modules and auxiliary modules according to their roles in reaction routes.We highlight four main‐reaction mod‐ules that can perform carbon metabolism,carbon assimilation,protein glycosylation and nonribo‐somal peptide synthesis,and exemplify auxiliary modules used for energy supply,protection and reinforcement for main reactions.The reactor‐level modularization of multienzyme catalysis is also discussed.
