Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermed...Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed.展开更多
The corrosion inhibition performance of co-immobilized lysozyme and lipase was investigated in a recirculating cooling water system. Four methods were carried out in co-immobilization, and the operating parameters wer...The corrosion inhibition performance of co-immobilized lysozyme and lipase was investigated in a recirculating cooling water system. Four methods were carried out in co-immobilization, and the operating parameters were optimized by using the respond surface methodology(RSM). The corrosion inhibition performance of co-immobilized lipase and lysozyme was evaluated by weight loss measurements and electrochemical measurements. The results revealed that the optimal co-immobilization method should be the sequential immobilization of lysozyme and then lipase. The inhibition efficiency was 86.10% under the optimal co-immobilized conditions. Electrochemical data showed that co-immobilized lysozyme and lipase was a mixed-type inhibitor and the corrosion inhibition efficiency was 81%.展开更多
In order to improve the stability and corrosion inhibition performance of bioenzyme, lipase and lsozyme were co-immobilized on the mesoporous molecular sieve MCM-41 by the adsorption method. Then the immobilized enzym...In order to improve the stability and corrosion inhibition performance of bioenzyme, lipase and lsozyme were co-immobilized on the mesoporous molecular sieve MCM-41 by the adsorption method. Then the immobilized enzymes were combined with amino trimethylene phosphonic acid and polyaspartic acid to prevent corrosion caused by circulating cooling water. The weight-loss method and electrochemical techniques were used to evaluate the performance of composite inhibitors. The co-immobilized lysozyme and lipase achieved good inhibition effects. After they were combined with amino trimethylene phosphonic acid and polyaspartic acid, the corrosion inhibition properties were further improved. The inhibition efficiency was promoted to 94.4%. During the corrosion inhibition process, the immobilized enzymes played an important role. The addition of corrosion inhibitor could inhibit the anodic dissolution and cathodic hydrogen evolution process of carbon steel at the same time. The adsorption of co-immobilized lysozyme and lipase composite inhibitor on the steel surface was a joint action involving physical adsorption and chemical adsorption.展开更多
The implementation of multiple enzymes for chemical production in a cell‐free scenario is an emerging field in biomanufacturing.It enables the redesign and reconstitution of new enzymatic routes for producing chemica...The implementation of multiple enzymes for chemical production in a cell‐free scenario is an emerging field in biomanufacturing.It enables the redesign and reconstitution of new enzymatic routes for producing chemicals that may be hard to obtain from natural pathways.Although the construction of a cell‐free multienzyme system is highly flexible and adaptable,it is challenging to make all enzymatic reactions act in concert.Recently,modular construction has been conceptual‐ized as an effective way to harmonize diverse enzymatic reactions.In this review,we introduce the concept of a multienzyme module and exemplify representative modules found in Nature.We then categorize recent developments of synthetic multienzyme modules into main‐reaction modules and auxiliary modules according to their roles in reaction routes.We highlight four main‐reaction mod‐ules that can perform carbon metabolism,carbon assimilation,protein glycosylation and nonribo‐somal peptide synthesis,and exemplify auxiliary modules used for energy supply,protection and reinforcement for main reactions.The reactor‐level modularization of multienzyme catalysis is also discussed.展开更多
基金supported by the National Natural Science Foundation of China(21878324,21706273)the CAS Pioneer Hundred Talent Program(Type C,reference#2016-081)。
文摘Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed.
基金financially supported by the National Natural Science Foundation of China (project 21077133)the Natural Foundation of Shandong Province and the Top Talent Project of China University of Petroleum (16RC17040003)
文摘The corrosion inhibition performance of co-immobilized lysozyme and lipase was investigated in a recirculating cooling water system. Four methods were carried out in co-immobilization, and the operating parameters were optimized by using the respond surface methodology(RSM). The corrosion inhibition performance of co-immobilized lipase and lysozyme was evaluated by weight loss measurements and electrochemical measurements. The results revealed that the optimal co-immobilization method should be the sequential immobilization of lysozyme and then lipase. The inhibition efficiency was 86.10% under the optimal co-immobilized conditions. Electrochemical data showed that co-immobilized lysozyme and lipase was a mixed-type inhibitor and the corrosion inhibition efficiency was 81%.
基金financially supported by Shandong Natural Science Foundation (ZR201702140013)
文摘In order to improve the stability and corrosion inhibition performance of bioenzyme, lipase and lsozyme were co-immobilized on the mesoporous molecular sieve MCM-41 by the adsorption method. Then the immobilized enzymes were combined with amino trimethylene phosphonic acid and polyaspartic acid to prevent corrosion caused by circulating cooling water. The weight-loss method and electrochemical techniques were used to evaluate the performance of composite inhibitors. The co-immobilized lysozyme and lipase achieved good inhibition effects. After they were combined with amino trimethylene phosphonic acid and polyaspartic acid, the corrosion inhibition properties were further improved. The inhibition efficiency was promoted to 94.4%. During the corrosion inhibition process, the immobilized enzymes played an important role. The addition of corrosion inhibitor could inhibit the anodic dissolution and cathodic hydrogen evolution process of carbon steel at the same time. The adsorption of co-immobilized lysozyme and lipase composite inhibitor on the steel surface was a joint action involving physical adsorption and chemical adsorption.
文摘The implementation of multiple enzymes for chemical production in a cell‐free scenario is an emerging field in biomanufacturing.It enables the redesign and reconstitution of new enzymatic routes for producing chemicals that may be hard to obtain from natural pathways.Although the construction of a cell‐free multienzyme system is highly flexible and adaptable,it is challenging to make all enzymatic reactions act in concert.Recently,modular construction has been conceptual‐ized as an effective way to harmonize diverse enzymatic reactions.In this review,we introduce the concept of a multienzyme module and exemplify representative modules found in Nature.We then categorize recent developments of synthetic multienzyme modules into main‐reaction modules and auxiliary modules according to their roles in reaction routes.We highlight four main‐reaction mod‐ules that can perform carbon metabolism,carbon assimilation,protein glycosylation and nonribo‐somal peptide synthesis,and exemplify auxiliary modules used for energy supply,protection and reinforcement for main reactions.The reactor‐level modularization of multienzyme catalysis is also discussed.
