BACKGROUND Metabolic dysfunction-associated fatty liver disease(MAFLD)is a severe threat to human health.Polygonum multiflorum(PM)has been proven to remedy mitochondria and relieve MAFLD,but the main pharmacodynamic i...BACKGROUND Metabolic dysfunction-associated fatty liver disease(MAFLD)is a severe threat to human health.Polygonum multiflorum(PM)has been proven to remedy mitochondria and relieve MAFLD,but the main pharmacodynamic ingredients for mitigating MAFLD remain unclear.AIM To research the active ingredients of PM adjusting mitochondria to relieve highfat diet(HFD)-induced MAFLD in rats.METHODS Fat emulsion-induced L02 adipocyte model and HFD-induced MAFLD rat model were used to investigate the anti-MAFLD ability of PM and explore their action mechanisms.The adipocyte model was also applied to evaluate the activities of PM-derived constituents in liver mitochondria from HFD-fed rats(mitochondrial pharmacology).PM-derived constituents in liver mitochondria were confirmed by ultra-high-performance liquid chromatography/mass spectrometry(mitochondrial pharmacochemistry).The abilities of PM-derived monomer and monomer groups were evaluated by the adipocyte model and MAFLD mouse model,respectively.RESULTS PM repaired mitochondrial ultrastructure and prevented oxidative stress and energy production disorder of liver mitochondria to mitigate fat emulsion-induced cellular steatosis and HFD-induced MAFLD.PM-derived constituents that entered the liver mitochondria inhibited oxidative stress damage and improved energy production against cellular steatosis.Eight chemicals were found in the liver mitochondria of PM-administrated rats.The anti-steatosis ability of one monomer and the anti-MAFLD activity of the monomer group were validated.CONCLUSION PM restored mitochondrial structure and function and alleviated MAFLD,which may be associated with the remedy of oxidative stress and energy production.The identified eight chemicals may be the main bioactive ingredients in PM that adjusted mitochondria to prevent MAFLD.Thus,PM provides a new approach to prevent MAFLD-related mitochondrial dysfunction.Mitochondrial pharmacology and pharmacochemistry further showed efficient strategies for determining the bioactive ingredients of Chinese medicines that adjust mitochondria to prevent diseases.展开更多
BACKGROUND Polygonum multiflorum is one of the leading causes of herb-induced liver injury in China.HLA-B*35:01 is reported to be a potential biomarker of Polygonum multiflorum-induced liver injury(PM-DILI).However,li...BACKGROUND Polygonum multiflorum is one of the leading causes of herb-induced liver injury in China.HLA-B*35:01 is reported to be a potential biomarker of Polygonum multiflorum-induced liver injury(PM-DILI).However,little is known about the relationship between single-nucleotide polymorphisms(SNPs) and PM-DILI.AIM To identify SNPs that indicate susceptibility to PM-DILI METHODS We conducted a systematic study enrolling 382 participants from four independent hospitals,including 73 PM-DILI patients,118 patients with other drug-induced liver injury(other-DILI) and 191 healthy controls.Whole-exome sequencing was performed for 8 PM-DILI patients and 8 healthy controls who were randomly selected from the above subjects.Nineteen SNPs that showed high frequencies in the 8 PM-DILI patients were selected as candidate SNPs and then screened in 65 PM-DILI patients,118 other-DILI patients and 183 healthy controls using the MassARRAY system.HLA-B high-resolution genotyping was performed for the 73 PM-DILI and 118 other-DILI patients.The Han-MHC database was selected as a population control for HLA-B analysis.P <6.25 x 103 after Bolferroni correction was considered significant.RESULTS The frequencies of rslll686806 in the HLA-A gene,rs1055348 in the HLA-B gene,and rs202047044 in the HLA-DRB1 gene were significantly higher in the PM-DILI group than in the control group [27.2% vs 11.6%,P=1.72×105,odds ratio(OR)=3.96,95% confidence interval(Cl):2.21-7.14;42.5% vs 8.6%,P=1.72×10-19 OR=13.62,95% CI:7.16-25.9;22.9% vs 8.1%,P=4.64×106,OR=4.1,95% CI:2.25-7.47].Only rs1055348 showed a significantly higher frequency in the PM-DILI group than in the other-DILI group(42.5% vs 13.6%,P=1.84×10-10,OR=10.06,95% Cl:5.06-20.0),which suggested that it is a specific risk factor for PM-DILI.rs1055348 may become a tag for HLA-B*35:01 with 100% sensitivity and 97.7% specificity in the PM-DILI group and 100% sensitivity and 98.1% specificity in the other-DILI group.Furthermore,HLA-B*35:01 was confirmed to be associated with PM-DILI with a frequency of 41.1% in the PM-DILI group compared with 11.9%(P=4.30×10-11,OR=11.11,95% CI:5.57-22.19) in the other-DILI group and 2.7%(P=6.22×10-166,OR=62.62,95% Cl:35.91-109.20) in the Han-MHC database.CONCLUSION rslll686806,rs1055348,and rs202047044 are associated with PM-DILI,of which,rs1055348 is specific to PM-DILI.As a tag for HLA-B*35:01,rs1055348 may become an alternative predictive biomarker of PM-DILI.展开更多
The glycosylation of hydroxylcoumarin was investigated by using suspension cultures of hairy roots of Polygonum multiflorum. Two new coumarin glucosides （3 and 4） were biosysthesized by regioselectively glycosylatio...The glycosylation of hydroxylcoumarin was investigated by using suspension cultures of hairy roots of Polygonum multiflorum. Two new coumarin glucosides （3 and 4） were biosysthesized by regioselectively glycosylation of corresponding substrates （1 and 2） in the system. The structures of two products were identified as 7-hydroxy-4-methylcoumarin 5-O-β-D-glucopyranoside and 7- hydroxy-3,4-dimethylcoumarin 5-O-β-D-glucopyranoside on the ground of chemical and spectroscopic methods, respectively. 2007 Rong Min Yu. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
BACKGROUND Various types of drug-induced liver injury are induced by Polygonum multiflorum(PM);however,it rarely causes neutropenia.Herein,we report the case of a 65-year-old woman with concurrent severe hepatotoxicit...BACKGROUND Various types of drug-induced liver injury are induced by Polygonum multiflorum(PM);however,it rarely causes neutropenia.Herein,we report the case of a 65-year-old woman with concurrent severe hepatotoxicity and agranulocytosis induced by PM.CASE SUMMARY A 65-year-old woman reported with severe hepatotoxicity and agranulocytosis 17 d after ingestion of PM.The results of the Roussel Uclaf Causality Assessment Method demonstrated a highly probable relationship between hepatotoxicity and PM,with a total score of 10.The Naranjo algorithm results indicated that agranulocytosis had a probable relationship with PM,with an overall score of 6.Granulocyte colony-stimulating factor(for once),a steroid,compound glycyrrhizin,and polyene phosphatidylcholine therapy were initiated.After 15 d of treatment,there was a gradual improvement in liver biochemistry,leukocytes,and neutrophils levels.CONCLUSION Concurrent hepatotoxicity and agranulocytosis are rare and critical adverse drug reactions of PM,which should be highly valued.展开更多
[Objectives]This study aimed to optimize the preparation process of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.[Methods]Taking the comprehensive weighed score of total flavonoid content,soup color,ar...[Objectives]This study aimed to optimize the preparation process of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.[Methods]Taking the comprehensive weighed score of total flavonoid content,soup color,aroma and taste as an indicator,the preparation process of tea bags was optimized using an orthogonal test.[Results]The results of the orthogonal test show that the optimal preparation process for the Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags are as follows:particle size of 14 meshes,solid to liquid ratio of 1∶40,brewing temperature of 70℃,and brewing time of 10 min.[Conclusions]The optimized preparation process is reasonable and feasible.It is suitable for the production of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.展开更多
Italian ryegrass is an annual/biennial grass that is typically used as a pasture crop or a cover crop along roadsides, rights-of-way, and industrial areas. Glyphosate-resistant (GR) Italian ryegrass populations have b...Italian ryegrass is an annual/biennial grass that is typically used as a pasture crop or a cover crop along roadsides, rights-of-way, and industrial areas. Glyphosate-resistant (GR) Italian ryegrass populations have been documented around the world, mostly in orchard and vineyard situations. The first evidence of evolved GR Italian ryegrass in row/agronomic crops was reported from Washington County, Mississippi in 2005. GR Italian ryegrass populations can jeopardize preplant burndown options in reduced-tillage crop production systems, thereby, delaying planting operations. The effects of competition of Italian ryegrass on crop growth and yield are poorly understood. A field study was conducted in the 2012 growing season and repeated in the 2013 growing season. GR and susceptible (GS) Italian ryegrass populations were established in the greenhouse and transplanted in prepared corn row beds in the fall of 2011 and 2012 at 0, 1, 2, 3, and 4 plants·meter> of crop row. Italian ryegrass plants overwintered and developed over the following spring-summer. Glyphosate was applied at 1.26 kg·ae/ha (1.5× of labeled rate) in the spring to burndown the Italian ryegrass plants and corn was planted into the ryegrass residue 2 - 3 wk later. Current corn production practices were followed. Corn density (early and late season), height (early season), and yield and Italian ryegrass biomass (early-mid season) measurements were recorded during both years. Corn height was greater in 2012 than that in 2013 at comparable stages of the growing season, due to a cooler and wetter early season in 2013 than that in 2012. Averaged across weed densities, corn density (both early and late season) and yield were higher in the GS than those in the GR population, but Italian ryegrass biomass was similar for both populations. Averaged across Italian ryegrass populations, corn density (both early and late season), and yield were inversely proportional to Italian ryegrass density. In summary, Italian ryegrass significantly reduced corn density and yield and reduction was greater with the GR than that with the GS population. Studies are underway to study inter population competition in Italian ryegrass and investigate allelopathic effects of Italian ryegrass on selected crops.展开更多
Use of individual plants as experimental units may be necessary when resources are limited, but inter-plant variation risks obscuring differences among treatments. Experiments were undertaken to measure the effects of...Use of individual plants as experimental units may be necessary when resources are limited, but inter-plant variation risks obscuring differences among treatments. Experiments were undertaken to measure the effects of seed size on seedling size and response to applied nitrogen of annual ryegrass (Lolium multiflorum L.) harvested six or nine weeks after emergence. In two series of experiments, shoot and root dry weights of seedlings increased respectively at means of 53 - 61 and 29 - 30 mg·mg-1 increase in mean seed weight. Between the largest and smallest seed sizes used in this study, there was 83% difference in shoot weight at harvest six weeks after emergence and 22% difference with harvest nine weeks after emergence. Nitrogen (N) application, in comparison, increased shoot dry weights by averages of 69% and 77% with harvests at six or nine weeks, respectively. Dry mass response to N application was similar across seed size categories in shoots or roots. A mean 30% of total seedling-N was retained in roots. Nitrogen utilization efficiency for shoot growth (increase in shoot growth per unit increase in shoot N capture) ranged from 64 mg·mg-1 with harvest six weeks after emergence to 114 mg·mg-1 with harvest at nine weeks after emergence. Delay in seedling harvest from six to nine weeks post-emergence and use of closely-graded seed can reduce variation in individual seedling size and contribute to reduction in random variation in small-scale experiments.展开更多
In 2021,there are approximately 537 million adults ageing 20-79 years affected by diabetes worldwide and the number is rising rapidly,hence it is important to manage and control diabetes mellitus and its associated co...In 2021,there are approximately 537 million adults ageing 20-79 years affected by diabetes worldwide and the number is rising rapidly,hence it is important to manage and control diabetes mellitus and its associated complications.Food is one of the key factors in preventing and combating diseases such as diabetes.Both as a food and an herbal medicine,Polygonum multiflorum(PM)has been used as an anti-aging tonic,for hair darkening in traditional Chinese medicine for several centuries.The recent research effort of PM has been focused on antioxidant,anti-ageing and anti-tumor properties.In the present study,we utilized the traditional processing of harvested raw PM,and identified several stilbene components and then evaluated the potential anti-diabetic effects of the processed PM extract(PME).PME(0.075%)was given to diabetic mice(KK CgAy/J)in drinking water and after 7 weeks,PME-treated mice had significantly lower glucose levels than mice in the diabetic control group(P<0.01).The mechanism was explored with ELISA and Western blotting and results suggested that the effect was through maintainingβ-cell function.展开更多
Three new jatropholane-type diterpenoids,jatropholones C-E(1-3),along with seven other known compounds,including sikkimenoid B(4),jatrophaldehyde(5),epi-jatrophaldehyde(6),epi-jatrophol(7),jatrophol(8),jatropholone A(...Three new jatropholane-type diterpenoids,jatropholones C-E(1-3),along with seven other known compounds,including sikkimenoid B(4),jatrophaldehyde(5),epi-jatrophaldehyde(6),epi-jatrophol(7),jatrophol(8),jatropholone A(9),and jatropholone B(10),were isolated from the roots of a natural cultivar of Jatropha curcas(J.curcas cv.Multiflorum CY Yang).The structural elucidations of 1-3 were accomplished by extensive NMR analysis.Compounds 4,6,and 8 demonstrated inhibition activity against the microorganisms with the MIC values from 0.10 to 0.18 mg/mL.展开更多
基金the National Natural Science Foundation of China,No.82060707 and 82104381the Application and Basis Research Project of Yunnan China,No.202201AW070016,202001AZ070001-006,and 2019IB009the Young and Middle-aged Academic and Technological Leader of Yunnan,No.202005AC160059.
文摘BACKGROUND Metabolic dysfunction-associated fatty liver disease(MAFLD)is a severe threat to human health.Polygonum multiflorum(PM)has been proven to remedy mitochondria and relieve MAFLD,but the main pharmacodynamic ingredients for mitigating MAFLD remain unclear.AIM To research the active ingredients of PM adjusting mitochondria to relieve highfat diet(HFD)-induced MAFLD in rats.METHODS Fat emulsion-induced L02 adipocyte model and HFD-induced MAFLD rat model were used to investigate the anti-MAFLD ability of PM and explore their action mechanisms.The adipocyte model was also applied to evaluate the activities of PM-derived constituents in liver mitochondria from HFD-fed rats(mitochondrial pharmacology).PM-derived constituents in liver mitochondria were confirmed by ultra-high-performance liquid chromatography/mass spectrometry(mitochondrial pharmacochemistry).The abilities of PM-derived monomer and monomer groups were evaluated by the adipocyte model and MAFLD mouse model,respectively.RESULTS PM repaired mitochondrial ultrastructure and prevented oxidative stress and energy production disorder of liver mitochondria to mitigate fat emulsion-induced cellular steatosis and HFD-induced MAFLD.PM-derived constituents that entered the liver mitochondria inhibited oxidative stress damage and improved energy production against cellular steatosis.Eight chemicals were found in the liver mitochondria of PM-administrated rats.The anti-steatosis ability of one monomer and the anti-MAFLD activity of the monomer group were validated.CONCLUSION PM restored mitochondrial structure and function and alleviated MAFLD,which may be associated with the remedy of oxidative stress and energy production.The identified eight chemicals may be the main bioactive ingredients in PM that adjusted mitochondria to prevent MAFLD.Thus,PM provides a new approach to prevent MAFLD-related mitochondrial dysfunction.Mitochondrial pharmacology and pharmacochemistry further showed efficient strategies for determining the bioactive ingredients of Chinese medicines that adjust mitochondria to prevent diseases.
基金supported by the Natural Science Foundation of Beijing (7232321)the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine (ZYYCXTD-C-202005)the State Key Program of National Natural Science of China (81930110,82230118)。
基金the National Natural Science Foundation of China,No.81470849the China Mega-Project for Infectious Diseases,No.2017ZX10203202.
文摘BACKGROUND Polygonum multiflorum is one of the leading causes of herb-induced liver injury in China.HLA-B*35:01 is reported to be a potential biomarker of Polygonum multiflorum-induced liver injury(PM-DILI).However,little is known about the relationship between single-nucleotide polymorphisms(SNPs) and PM-DILI.AIM To identify SNPs that indicate susceptibility to PM-DILI METHODS We conducted a systematic study enrolling 382 participants from four independent hospitals,including 73 PM-DILI patients,118 patients with other drug-induced liver injury(other-DILI) and 191 healthy controls.Whole-exome sequencing was performed for 8 PM-DILI patients and 8 healthy controls who were randomly selected from the above subjects.Nineteen SNPs that showed high frequencies in the 8 PM-DILI patients were selected as candidate SNPs and then screened in 65 PM-DILI patients,118 other-DILI patients and 183 healthy controls using the MassARRAY system.HLA-B high-resolution genotyping was performed for the 73 PM-DILI and 118 other-DILI patients.The Han-MHC database was selected as a population control for HLA-B analysis.P <6.25 x 103 after Bolferroni correction was considered significant.RESULTS The frequencies of rslll686806 in the HLA-A gene,rs1055348 in the HLA-B gene,and rs202047044 in the HLA-DRB1 gene were significantly higher in the PM-DILI group than in the control group [27.2% vs 11.6%,P=1.72×105,odds ratio(OR)=3.96,95% confidence interval(Cl):2.21-7.14;42.5% vs 8.6%,P=1.72×10-19 OR=13.62,95% CI:7.16-25.9;22.9% vs 8.1%,P=4.64×106,OR=4.1,95% CI:2.25-7.47].Only rs1055348 showed a significantly higher frequency in the PM-DILI group than in the other-DILI group(42.5% vs 13.6%,P=1.84×10-10,OR=10.06,95% Cl:5.06-20.0),which suggested that it is a specific risk factor for PM-DILI.rs1055348 may become a tag for HLA-B*35:01 with 100% sensitivity and 97.7% specificity in the PM-DILI group and 100% sensitivity and 98.1% specificity in the other-DILI group.Furthermore,HLA-B*35:01 was confirmed to be associated with PM-DILI with a frequency of 41.1% in the PM-DILI group compared with 11.9%(P=4.30×10-11,OR=11.11,95% CI:5.57-22.19) in the other-DILI group and 2.7%(P=6.22×10-166,OR=62.62,95% Cl:35.91-109.20) in the Han-MHC database.CONCLUSION rslll686806,rs1055348,and rs202047044 are associated with PM-DILI,of which,rs1055348 is specific to PM-DILI.As a tag for HLA-B*35:01,rs1055348 may become an alternative predictive biomarker of PM-DILI.
文摘The glycosylation of hydroxylcoumarin was investigated by using suspension cultures of hairy roots of Polygonum multiflorum. Two new coumarin glucosides （3 and 4） were biosysthesized by regioselectively glycosylation of corresponding substrates （1 and 2） in the system. The structures of two products were identified as 7-hydroxy-4-methylcoumarin 5-O-β-D-glucopyranoside and 7- hydroxy-3,4-dimethylcoumarin 5-O-β-D-glucopyranoside on the ground of chemical and spectroscopic methods, respectively. 2007 Rong Min Yu. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
基金Supported by Applied Basic Research Project of Changzhou,No.CJ20190080。
文摘BACKGROUND Various types of drug-induced liver injury are induced by Polygonum multiflorum(PM);however,it rarely causes neutropenia.Herein,we report the case of a 65-year-old woman with concurrent severe hepatotoxicity and agranulocytosis induced by PM.CASE SUMMARY A 65-year-old woman reported with severe hepatotoxicity and agranulocytosis 17 d after ingestion of PM.The results of the Roussel Uclaf Causality Assessment Method demonstrated a highly probable relationship between hepatotoxicity and PM,with a total score of 10.The Naranjo algorithm results indicated that agranulocytosis had a probable relationship with PM,with an overall score of 6.Granulocyte colony-stimulating factor(for once),a steroid,compound glycyrrhizin,and polyene phosphatidylcholine therapy were initiated.After 15 d of treatment,there was a gradual improvement in liver biochemistry,leukocytes,and neutrophils levels.CONCLUSION Concurrent hepatotoxicity and agranulocytosis are rare and critical adverse drug reactions of PM,which should be highly valued.
基金Supported by Development and Research Center for China-ASEAN Traditional Medicine(05018017)Natural Science Foundation of Guangxi(2014GXNSFBA118195)
文摘[Objectives]This study aimed to optimize the preparation process of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.[Methods]Taking the comprehensive weighed score of total flavonoid content,soup color,aroma and taste as an indicator,the preparation process of tea bags was optimized using an orthogonal test.[Results]The results of the orthogonal test show that the optimal preparation process for the Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags are as follows:particle size of 14 meshes,solid to liquid ratio of 1∶40,brewing temperature of 70℃,and brewing time of 10 min.[Conclusions]The optimized preparation process is reasonable and feasible.It is suitable for the production of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.
文摘Italian ryegrass is an annual/biennial grass that is typically used as a pasture crop or a cover crop along roadsides, rights-of-way, and industrial areas. Glyphosate-resistant (GR) Italian ryegrass populations have been documented around the world, mostly in orchard and vineyard situations. The first evidence of evolved GR Italian ryegrass in row/agronomic crops was reported from Washington County, Mississippi in 2005. GR Italian ryegrass populations can jeopardize preplant burndown options in reduced-tillage crop production systems, thereby, delaying planting operations. The effects of competition of Italian ryegrass on crop growth and yield are poorly understood. A field study was conducted in the 2012 growing season and repeated in the 2013 growing season. GR and susceptible (GS) Italian ryegrass populations were established in the greenhouse and transplanted in prepared corn row beds in the fall of 2011 and 2012 at 0, 1, 2, 3, and 4 plants·meter> of crop row. Italian ryegrass plants overwintered and developed over the following spring-summer. Glyphosate was applied at 1.26 kg·ae/ha (1.5× of labeled rate) in the spring to burndown the Italian ryegrass plants and corn was planted into the ryegrass residue 2 - 3 wk later. Current corn production practices were followed. Corn density (early and late season), height (early season), and yield and Italian ryegrass biomass (early-mid season) measurements were recorded during both years. Corn height was greater in 2012 than that in 2013 at comparable stages of the growing season, due to a cooler and wetter early season in 2013 than that in 2012. Averaged across weed densities, corn density (both early and late season) and yield were higher in the GS than those in the GR population, but Italian ryegrass biomass was similar for both populations. Averaged across Italian ryegrass populations, corn density (both early and late season), and yield were inversely proportional to Italian ryegrass density. In summary, Italian ryegrass significantly reduced corn density and yield and reduction was greater with the GR than that with the GS population. Studies are underway to study inter population competition in Italian ryegrass and investigate allelopathic effects of Italian ryegrass on selected crops.
文摘Use of individual plants as experimental units may be necessary when resources are limited, but inter-plant variation risks obscuring differences among treatments. Experiments were undertaken to measure the effects of seed size on seedling size and response to applied nitrogen of annual ryegrass (Lolium multiflorum L.) harvested six or nine weeks after emergence. In two series of experiments, shoot and root dry weights of seedlings increased respectively at means of 53 - 61 and 29 - 30 mg·mg-1 increase in mean seed weight. Between the largest and smallest seed sizes used in this study, there was 83% difference in shoot weight at harvest six weeks after emergence and 22% difference with harvest nine weeks after emergence. Nitrogen (N) application, in comparison, increased shoot dry weights by averages of 69% and 77% with harvests at six or nine weeks, respectively. Dry mass response to N application was similar across seed size categories in shoots or roots. A mean 30% of total seedling-N was retained in roots. Nitrogen utilization efficiency for shoot growth (increase in shoot growth per unit increase in shoot N capture) ranged from 64 mg·mg-1 with harvest six weeks after emergence to 114 mg·mg-1 with harvest at nine weeks after emergence. Delay in seedling harvest from six to nine weeks post-emergence and use of closely-graded seed can reduce variation in individual seedling size and contribute to reduction in random variation in small-scale experiments.
基金supported by Hubei Science and Technology Plan key Project,Hubei Province,China(2019ABA100)。
文摘In 2021,there are approximately 537 million adults ageing 20-79 years affected by diabetes worldwide and the number is rising rapidly,hence it is important to manage and control diabetes mellitus and its associated complications.Food is one of the key factors in preventing and combating diseases such as diabetes.Both as a food and an herbal medicine,Polygonum multiflorum(PM)has been used as an anti-aging tonic,for hair darkening in traditional Chinese medicine for several centuries.The recent research effort of PM has been focused on antioxidant,anti-ageing and anti-tumor properties.In the present study,we utilized the traditional processing of harvested raw PM,and identified several stilbene components and then evaluated the potential anti-diabetic effects of the processed PM extract(PME).PME(0.075%)was given to diabetic mice(KK CgAy/J)in drinking water and after 7 weeks,PME-treated mice had significantly lower glucose levels than mice in the diabetic control group(P<0.01).The mechanism was explored with ELISA and Western blotting and results suggested that the effect was through maintainingβ-cell function.
基金This work was financially supported by the Key Projects of the Chinese Ministry of Science and Technology(2007BAD32B01-03 and SB2007FY400)the National Natural Science Foundation of China(81202437)the National Knowledge Innovation Program of Chinese Academy of Sciences(KSCX2-YW-G-038).
文摘Three new jatropholane-type diterpenoids,jatropholones C-E(1-3),along with seven other known compounds,including sikkimenoid B(4),jatrophaldehyde(5),epi-jatrophaldehyde(6),epi-jatrophol(7),jatrophol(8),jatropholone A(9),and jatropholone B(10),were isolated from the roots of a natural cultivar of Jatropha curcas(J.curcas cv.Multiflorum CY Yang).The structural elucidations of 1-3 were accomplished by extensive NMR analysis.Compounds 4,6,and 8 demonstrated inhibition activity against the microorganisms with the MIC values from 0.10 to 0.18 mg/mL.
