A cell line designated as Ca 761-86 has been established from the solid mouse mammary cancer (Ca 761) by suspension culture. It has been passaged for more than 212 generations. Moderate round cells were predominant an...A cell line designated as Ca 761-86 has been established from the solid mouse mammary cancer (Ca 761) by suspension culture. It has been passaged for more than 212 generations. Moderate round cells were predominant and most of them were mononuclear. Some characteristics of malignant cells and A-type viral-like particles were observed by electron microscopy. The results of cytochemical studies (DNA, RNA, SDH, 5' AMPase, ACP etc.) were comparable to the ultramicroscopic results. It multiplied approximately 27.4 fold on day 5 with mitotic index reaching 1.8% on day 3. This cell line was a hyperdiploid with karyotype of 45 or 45, -2X, tril2, tri17, +M1-5. Cell agglutination was observed when treated with ConA (≥7 fig, ml). Spontaneous agglutination might also take place without adding any ConA. After 5×106 cells of Ca 761-86 suspension were transplanted into the normal inbred 615 mice by different ways (subcutan eous, intrafoot-pad or intraperitoneal), the transplan lability rate reached 100%. Spontaneous remission was never observed and its metastatic ability reserved. PPLO were not detected. Ca 761-86 may be of value for practical purposes.展开更多
NIH/3T3 cells were infected with a cell-free extract from the mouse SRS ascltic tumor and propagated at vitro. Experimental results showed that type A and type C virus particles were observed in the cytoplasm of infec...NIH/3T3 cells were infected with a cell-free extract from the mouse SRS ascltic tumor and propagated at vitro. Experimental results showed that type A and type C virus particles were observed in the cytoplasm of infected NIH/ 3T3 cells by electron-microscopy, X-C assay and reverse transcriptase were both positive. Free proviral-DN A of the leukemia vims was found in the infected NIH/3T3 cell by Southern blot hybridization.Morphologically, the NIH/3T3 cells infected in vitro appeared spherical and formed monolayer cell colonies of various sizes after 24 - 48 hours. When the cultured cells were Inoculated into nude mice (BALB/ c, nu nu ) subcutaneously, flbrosarcoma was Induced, 100%. Inoculation of the cell-free extract of the same cultured cells into Inbred SW-1 newborn mice, resulted in the production of lymphocytic leukemia and lymphoma in 52. 3% within 191 days.展开更多
Having been passed for 160 generations, a cell linedesignated as H22-F25/L was established from a murine tumorlymphatic metastatlc model H22-F25 which had been set up in our college. The cell line was in suspension cu...Having been passed for 160 generations, a cell linedesignated as H22-F25/L was established from a murine tumorlymphatic metastatlc model H22-F25 which had been set up in our college. The cell line was in suspension culture with a rapid proliferation and stable growth. The peak tune of cell division and proliferation was 48 and 96 hours after culture. In a week, the cell number was Increased by 25 tunes. H22-F25/L still keeps the features of a poorly differentiated cancer. Its tumor inducing rate (in vivo)was 100% in 615 mice. Lymph node metastasis rate was 50% and pulmonary metastasis rate 10%. H22- F25/ L Is a population of heterogenetlc tumor cells Including 2 stem cell lines (the model number of chromosomes being 43 in 40% tumor cells and 86 in 32%) and some side lines. The common marker chromosomes M1, M2, M3 and M4 were present in all stem and side lines.展开更多
Background There were only 3 multiple myeloma (MM) cell lines established in China. In this study,we succeeded in establishing a novel MM cell line and analyzed its biological characteristics. Methods Mononuclear...Background There were only 3 multiple myeloma (MM) cell lines established in China. In this study,we succeeded in establishing a novel MM cell line and analyzed its biological characteristics. Methods Mononuclear cells isolated from the peripheral blood (PB) and bone marrow (BM) of a patient with advanced MM (λ light chain type) were cultured in medium. Cell morphology was analyzed by Wright-Giemsa-staining and cytochemical staining,immunophenotyping by flow cytometry and cytogenetic analysis by chromosome RHG-banding technique. Quantitative fluorescent polymerase chain reaction (PCR) was used to detect Epstein - Barr virus (EBV) DNA. Results The established cell line could survive and proliferate in the presence of feeder cells or conditioned medium. The cells secreted λ light chain and were negative for EBV. The Wright-Giemsa-staining showed typical plasmablast or plasma cell morphology. The cytochemical staining of the cells showed the following reactivity patterns: positive for acid phosphatase,negative for myeloperoxidase. The immunoprofile of the cells was concordant with that of MM cells: positive for CD_ 10 ,CD_ 28 ,CD_ 38 ,CD_ 138 ,CD_ 56 ,CD_ 49d ,CD_ 44 ,CD_ 54 and CD_ 58 ,negative for CD_ 19 , CD_ 40 ,CD_ 95 ,CD_ 95L ,CD_ 34 ,CD_2 and CD_5. The cytogenetic analysis showed complex chromosome abnormality of i (1q+),8q+,13q+,i (17q),i (18q) and +M. There was no difference in morphology,immunophenotype and cytogenetics between cells from PB and BM. Conclusions An MM cell line secreting λ light chain named CZ-1 was established. The cells from both PB and BM have the same biological characteristics.展开更多
A Yunnan pony ear marginal tissue fibroblast cell line (NYPEM 2/2) was successfully established using the explant of the ear marginal tissue and then trypsinization the cells from the outgrowth. Observations on cell m...A Yunnan pony ear marginal tissue fibroblast cell line (NYPEM 2/2) was successfully established using the explant of the ear marginal tissue and then trypsinization the cells from the outgrowth. Observations on cell morphology and dynamic growth, analysis of karyotype and isoenzymes of lactate dehydrogenase and malate dehydrogenase were carried out. The expression of recombinant green fluorescence protein in the cells were also undertaken. The results showed that the population doubling time (PDT) of the cells was 24 h; the frequency of cell chromosome number to be 2n=64 was 92.9%; the banding patterns of the isozymes of the two enzymes had significant difference between the Yunnan pony ear marginal fibroblast cell line and the fibroblast cell lines of PEM 2/2, MSHEM 2/2 and BLCHE 2/2 derived from the Picdmont bovine ear, Mongolian ovine ear and Beijing local chicken embryo respectively. Tests for the contamination from bacteria, fungi or mycoplasma were negative; the transfection efficiency for the recombinant plasmid was 32.3%. This newly established cell line make the Yunnan pony breed, a national important genetic resource preserved at cell level, as well as will provide an effective experimental material for genetic studies on the Yunnan pony.展开更多
文摘A cell line designated as Ca 761-86 has been established from the solid mouse mammary cancer (Ca 761) by suspension culture. It has been passaged for more than 212 generations. Moderate round cells were predominant and most of them were mononuclear. Some characteristics of malignant cells and A-type viral-like particles were observed by electron microscopy. The results of cytochemical studies (DNA, RNA, SDH, 5' AMPase, ACP etc.) were comparable to the ultramicroscopic results. It multiplied approximately 27.4 fold on day 5 with mitotic index reaching 1.8% on day 3. This cell line was a hyperdiploid with karyotype of 45 or 45, -2X, tril2, tri17, +M1-5. Cell agglutination was observed when treated with ConA (≥7 fig, ml). Spontaneous agglutination might also take place without adding any ConA. After 5×106 cells of Ca 761-86 suspension were transplanted into the normal inbred 615 mice by different ways (subcutan eous, intrafoot-pad or intraperitoneal), the transplan lability rate reached 100%. Spontaneous remission was never observed and its metastatic ability reserved. PPLO were not detected. Ca 761-86 may be of value for practical purposes.
文摘NIH/3T3 cells were infected with a cell-free extract from the mouse SRS ascltic tumor and propagated at vitro. Experimental results showed that type A and type C virus particles were observed in the cytoplasm of infected NIH/ 3T3 cells by electron-microscopy, X-C assay and reverse transcriptase were both positive. Free proviral-DN A of the leukemia vims was found in the infected NIH/3T3 cell by Southern blot hybridization.Morphologically, the NIH/3T3 cells infected in vitro appeared spherical and formed monolayer cell colonies of various sizes after 24 - 48 hours. When the cultured cells were Inoculated into nude mice (BALB/ c, nu nu ) subcutaneously, flbrosarcoma was Induced, 100%. Inoculation of the cell-free extract of the same cultured cells into Inbred SW-1 newborn mice, resulted in the production of lymphocytic leukemia and lymphoma in 52. 3% within 191 days.
文摘Having been passed for 160 generations, a cell linedesignated as H22-F25/L was established from a murine tumorlymphatic metastatlc model H22-F25 which had been set up in our college. The cell line was in suspension culture with a rapid proliferation and stable growth. The peak tune of cell division and proliferation was 48 and 96 hours after culture. In a week, the cell number was Increased by 25 tunes. H22-F25/L still keeps the features of a poorly differentiated cancer. Its tumor inducing rate (in vivo)was 100% in 615 mice. Lymph node metastasis rate was 50% and pulmonary metastasis rate 10%. H22- F25/ L Is a population of heterogenetlc tumor cells Including 2 stem cell lines (the model number of chromosomes being 43 in 40% tumor cells and 86 in 32%) and some side lines. The common marker chromosomes M1, M2, M3 and M4 were present in all stem and side lines.
文摘Background There were only 3 multiple myeloma (MM) cell lines established in China. In this study,we succeeded in establishing a novel MM cell line and analyzed its biological characteristics. Methods Mononuclear cells isolated from the peripheral blood (PB) and bone marrow (BM) of a patient with advanced MM (λ light chain type) were cultured in medium. Cell morphology was analyzed by Wright-Giemsa-staining and cytochemical staining,immunophenotyping by flow cytometry and cytogenetic analysis by chromosome RHG-banding technique. Quantitative fluorescent polymerase chain reaction (PCR) was used to detect Epstein - Barr virus (EBV) DNA. Results The established cell line could survive and proliferate in the presence of feeder cells or conditioned medium. The cells secreted λ light chain and were negative for EBV. The Wright-Giemsa-staining showed typical plasmablast or plasma cell morphology. The cytochemical staining of the cells showed the following reactivity patterns: positive for acid phosphatase,negative for myeloperoxidase. The immunoprofile of the cells was concordant with that of MM cells: positive for CD_ 10 ,CD_ 28 ,CD_ 38 ,CD_ 138 ,CD_ 56 ,CD_ 49d ,CD_ 44 ,CD_ 54 and CD_ 58 ,negative for CD_ 19 , CD_ 40 ,CD_ 95 ,CD_ 95L ,CD_ 34 ,CD_2 and CD_5. The cytogenetic analysis showed complex chromosome abnormality of i (1q+),8q+,13q+,i (17q),i (18q) and +M. There was no difference in morphology,immunophenotype and cytogenetics between cells from PB and BM. Conclusions An MM cell line secreting λ light chain named CZ-1 was established. The cells from both PB and BM have the same biological characteristics.
基金国家基础性工作重大专项 (No . 2 0 0 1DEA10 0 0 6) 国家自然科学基金 (No . 3 0 10 0 13 2 )资助~~
文摘A Yunnan pony ear marginal tissue fibroblast cell line (NYPEM 2/2) was successfully established using the explant of the ear marginal tissue and then trypsinization the cells from the outgrowth. Observations on cell morphology and dynamic growth, analysis of karyotype and isoenzymes of lactate dehydrogenase and malate dehydrogenase were carried out. The expression of recombinant green fluorescence protein in the cells were also undertaken. The results showed that the population doubling time (PDT) of the cells was 24 h; the frequency of cell chromosome number to be 2n=64 was 92.9%; the banding patterns of the isozymes of the two enzymes had significant difference between the Yunnan pony ear marginal fibroblast cell line and the fibroblast cell lines of PEM 2/2, MSHEM 2/2 and BLCHE 2/2 derived from the Picdmont bovine ear, Mongolian ovine ear and Beijing local chicken embryo respectively. Tests for the contamination from bacteria, fungi or mycoplasma were negative; the transfection efficiency for the recombinant plasmid was 32.3%. This newly established cell line make the Yunnan pony breed, a national important genetic resource preserved at cell level, as well as will provide an effective experimental material for genetic studies on the Yunnan pony.
