Objective To investigate the expression of routine cytomegalovirus （MCMV） M83 mRNA in mouse testes at the different periods of MCMV infection and its relationship with the severity of testicular lesions Methods Used...Objective To investigate the expression of routine cytomegalovirus （MCMV） M83 mRNA in mouse testes at the different periods of MCMV infection and its relationship with the severity of testicular lesions Methods Used digoxin-labeled MCMV mRNA oligonucleotide probe, the mouse testicular tissues at the different periods following MCMV inoculation were detected by in situ hybridization（ISH）. The average absorbency （A） of ISH positive signals represented the resticular MCMV load. The expression of MCMV M83 mRNA in the infected testes and its relation with the severity of testicular lesions were analyzed. Results At different periods after MCMV inoculation, the expression of MCMV M83 mRNA was detectable in the testicular tissues. The ISH positive signals were mainly located in cytoplasm of the testicular interstitial cells. The average A values at d 9 after MCMV infection were the highest （comparison between the experimental groups, P〈O. O01）. There were pathological inJTammatory changes to varying degrees in seminiferous epithelia and interstitial cells at the diJferent periods of infection, especially during d 6 to d 9. Conclusion In testicular MCMV infection, the levels of MCMV M83 mRNA were related with the severity of testicular lesions, suggesting the dynamic detection of MCMV M83 mRNA could evaluate the prognosis of MCMV infection.展开更多
文摘Objective To investigate the expression of routine cytomegalovirus （MCMV） M83 mRNA in mouse testes at the different periods of MCMV infection and its relationship with the severity of testicular lesions Methods Used digoxin-labeled MCMV mRNA oligonucleotide probe, the mouse testicular tissues at the different periods following MCMV inoculation were detected by in situ hybridization（ISH）. The average absorbency （A） of ISH positive signals represented the resticular MCMV load. The expression of MCMV M83 mRNA in the infected testes and its relation with the severity of testicular lesions were analyzed. Results At different periods after MCMV inoculation, the expression of MCMV M83 mRNA was detectable in the testicular tissues. The ISH positive signals were mainly located in cytoplasm of the testicular interstitial cells. The average A values at d 9 after MCMV infection were the highest （comparison between the experimental groups, P〈O. O01）. There were pathological inJTammatory changes to varying degrees in seminiferous epithelia and interstitial cells at the diJferent periods of infection, especially during d 6 to d 9. Conclusion In testicular MCMV infection, the levels of MCMV M83 mRNA were related with the severity of testicular lesions, suggesting the dynamic detection of MCMV M83 mRNA could evaluate the prognosis of MCMV infection.
