An alcohol dehydrogenase （ADH）-coexisted solidstate electrochemiluminescence （ECL） biosensor for sensitive detection of the p53 gene was developed. The electrode modified by multiwalled carbon nanotubes, Ru（bpy...An alcohol dehydrogenase （ADH）-coexisted solidstate electrochemiluminescence （ECL） biosensor for sensitive detection of the p53 gene was developed. The electrode modified by multiwalled carbon nanotubes, Ru（bpy）]2＋3 and polypyrrole （ MWNTs-Ru （bpy） ]2＋3 -PPy ） was prepared to adsorb the ssDNA by electrostatic interactions. Then, the ssDNA recognized the gold nanoparticles （AuNPs）-labeled p53 gene and produced the AuNPs-dsDNA electrode with the AuNPs layer. The AuNPs layer adsorbed the ADH molecules for producing the ECL signal. Thus, the biosensor was based on coupling enzyme substrate reaction with solid-state ECL detection, and it displayed good sensitivity and specificity. The detection limit of the wild type p53 sequence （wtp53） is as low as 0. 1 pmol/L and the discrimination is up to 57. 1% between the wtp53 and the muted type p53 sequence （mtp53）. The amenability of this method to the analyses of p53 from normal and cancer cell lysates is demonstrated. The signal of wtp53 in the MGC-803 gastric cancer cell lysates turns out to be about 61.8% that of the wtp53 in the GES-1 normal gastric mucosal cell lysates, and the concentration of the wtp53 is found to decrease about 59 times. The method is highly complementary to enzyme-linked immunosorbent assay （ELISA）, and it holds promise for the diagnosis and management of cancer.展开更多
"S-E-T"是指:通过分析当前"社会、经济、技术"的状况,并预测未来发展趋势,进而找出现有产品和新趋势推动下所产生的需要或者改进可能性之间的缺口。以德国M U T E电动汽车为例,基于这三种因素对新产品机会进行了评..."S-E-T"是指:通过分析当前"社会、经济、技术"的状况,并预测未来发展趋势,进而找出现有产品和新趋势推动下所产生的需要或者改进可能性之间的缺口。以德国M U T E电动汽车为例,基于这三种因素对新产品机会进行了评估,并通过工业设计实现该机会。具体设计过程包括概念设计与造型设计,概念设计又包括车身工程设计、品牌规划两个阶段;造型设计部分又包括总体造型设计、标识性特征设计、细节设计三个阶段。最后对该方法进行了总结。展开更多
The goal of quality-of-service （QoS） multicast routing is to establish a multicast tree which satisfies certain constraints on bandwidth, delay and other metrics. The network state information maintained at every no...The goal of quality-of-service （QoS） multicast routing is to establish a multicast tree which satisfies certain constraints on bandwidth, delay and other metrics. The network state information maintained at every node is often im- precise in a dynamic environment because of non-negligible propagation delay of state messages, periodic updates due to overhead concern, and hierarchical state aggregation. The existing QoS multicast routing algorithms do not provide satisfactory performance with imprecise state information. We propose a distributed QoS multicast routing scheme based on traffic lights, called QMRI algorithm, which can probe multiple feasible tree branches, and select the optimal or near-optimal branch through the UR or TL mode for constructing a multicast tree with QoS guarantees if it exists. The scheme is designed to work with imprecise state information. The proposed algorithm considers not only the QoS requirements but also the cost optimality of the multicast tree. The correctness proof and the complexity analysis about the QMRI algorithm are also given. In addition, we develop NS2 so that it is able to simulate the imprecise network state information. Extensive simulations show that our algorithm achieves high call-admission ratio and low-cost multicast trees with modest message overhead.展开更多
Y2O2S：Eu nano crystallines were prepared by a new ethanol assisted combustion synthesis method using sulfurcontained organic fuel in an ethanol-aqueous solution. The as-prepared nanocrystallines were characterized by...Y2O2S：Eu nano crystallines were prepared by a new ethanol assisted combustion synthesis method using sulfurcontained organic fuel in an ethanol-aqueous solution. The as-prepared nanocrystallines were characterized by X-ray diffraction, transmission electron microscope, photoluminescence spectra and X-ray luminescence spectra. It is shown that the assistant fuel ethanol has the effect of decreasing the water needed, simplifying the experiment procedure by dissolving rare earth nitrate and sulfur-contained organic fuel into an even solution, and prompting the formation of rare earth oxysulfide by igniting firstly during heating that leads to combustion decomposition reaction. Y2O2S ： Eu nano crystallines with strong photoluminescence and X-ray luminescence are obtained using thioacetamide as organic fuel. Mixtures of Y2O3 ： Eu and Y2O2S ： Eu are acquired using thiourea as fuel, and the content of Y2O2S ： Eu increases until reaches to about half of the Y2O3 ： Eu with the increasing amount of thiourea. Y2O2SO4 ： Eu emerges when S/Y = 6 and increases with increasing thiourea amount.展开更多
In this paper, a distributed muting strategy based on simplified topology （DRBST） was proposed for LEO satellite networks. The topology of LEO satellite networks was simplified aiming at minimizing intersatellite li...In this paper, a distributed muting strategy based on simplified topology （DRBST） was proposed for LEO satellite networks. The topology of LEO satellite networks was simplified aiming at minimizing intersatellite links handover number. To optimize the route based on the simplified topology, we considered not only the transmission delay but also the queuing delay and the processing delay, which were analyzed using Markov chain and determined using a novel methodology. The DRBST algorithm was simulated in a LEO satellite networks model built using OPNET. The simulation results demonstrate that the low complexity DRBST algorithm can guarantee end-to-end delay bound. Moreover, the muting protocol cost is much less than traditional algorithms.展开更多
Geographic routing has been introduced in mobile ad hoc networks and sensor networks. But its per-formance suffers greatly from mobility-induced location errors that can cause Lost Link （LLNK） and LOOP problems. Thu...Geographic routing has been introduced in mobile ad hoc networks and sensor networks. But its per-formance suffers greatly from mobility-induced location errors that can cause Lost Link （LLNK） and LOOP problems. Thus various mobility prediction algorithms have been proposed to mitigate the errors, but sometimes their prediction errors are substantial. A novel mobility prediction technique that incorpo-rates both mobile positioning information and road topology knowledge was presented. Furthermore, the performance of the scheme was evaluated via simulations, along with two other schemes, namely, Linear Velocity Prediction （LVP） and Weighted Velocity Prediction （WVP） for comparison purpose. The results of simulation under Manhattan mobility model show that the proposed scheme could track the movement of a node well and hence provide noticeable improvement over LVP and MVP.展开更多
The dynamical-wave routing model of the urban unsteady and non-pressure rain pipe flow was established by conservation of mass, momentum and energy, and it was solved by applying the four point implicit difference met...The dynamical-wave routing model of the urban unsteady and non-pressure rain pipe flow was established by conservation of mass, momentum and energy, and it was solved by applying the four point implicit difference method and the pursuit method. It was obtained from the experiment checking and comparative analysis that the dynamical-wave muting model can reflect influence like attenuate and backwater when flood peak propagate in pipeline with high calculation precision and vast application scope, and it can be applied in routing of urban rain pipe flow of different slopes and inflow conditions. The routing model supplies a scientific foundation for the town rainfall piping design or checking, disaster administration of storm runoff, and so on.展开更多
The article proposes a network parameter-awareness （NPA） method and applies it to routing discovery algorithms in autonomic optical Internet. This NPA method can perceive the main parameters of the network, such as ...The article proposes a network parameter-awareness （NPA） method and applies it to routing discovery algorithms in autonomic optical Internet. This NPA method can perceive the main parameters of the network, such as delay, jitter and traffic, which can represent the current situation of the network. And these parameters enable network to determine the appropriate nodes for routing discovery. The simulation results of evaluating performance of a network with NPA method and its routing applications show that the method and its applications in routing improve the performance of the network significantly with quality of service （QoS） guaranteed.展开更多
基金The National Basic Research Program of China(973 Program)(No.2010CB732404,2011CB933404)the National Natural Science Foundation of China(No.81172697,81170492,81001244)+1 种基金the Specialized Research Fund for the Doctoral Program of Higher Education(No.20110092120055)the Foundation of the State Key Laboratory of Bioelectronics of Southeast University
文摘An alcohol dehydrogenase （ADH）-coexisted solidstate electrochemiluminescence （ECL） biosensor for sensitive detection of the p53 gene was developed. The electrode modified by multiwalled carbon nanotubes, Ru（bpy）]2＋3 and polypyrrole （ MWNTs-Ru （bpy） ]2＋3 -PPy ） was prepared to adsorb the ssDNA by electrostatic interactions. Then, the ssDNA recognized the gold nanoparticles （AuNPs）-labeled p53 gene and produced the AuNPs-dsDNA electrode with the AuNPs layer. The AuNPs layer adsorbed the ADH molecules for producing the ECL signal. Thus, the biosensor was based on coupling enzyme substrate reaction with solid-state ECL detection, and it displayed good sensitivity and specificity. The detection limit of the wild type p53 sequence （wtp53） is as low as 0. 1 pmol/L and the discrimination is up to 57. 1% between the wtp53 and the muted type p53 sequence （mtp53）. The amenability of this method to the analyses of p53 from normal and cancer cell lysates is demonstrated. The signal of wtp53 in the MGC-803 gastric cancer cell lysates turns out to be about 61.8% that of the wtp53 in the GES-1 normal gastric mucosal cell lysates, and the concentration of the wtp53 is found to decrease about 59 times. The method is highly complementary to enzyme-linked immunosorbent assay （ELISA）, and it holds promise for the diagnosis and management of cancer.
文摘"S-E-T"是指:通过分析当前"社会、经济、技术"的状况,并预测未来发展趋势,进而找出现有产品和新趋势推动下所产生的需要或者改进可能性之间的缺口。以德国M U T E电动汽车为例,基于这三种因素对新产品机会进行了评估,并通过工业设计实现该机会。具体设计过程包括概念设计与造型设计,概念设计又包括车身工程设计、品牌规划两个阶段;造型设计部分又包括总体造型设计、标识性特征设计、细节设计三个阶段。最后对该方法进行了总结。
文摘The goal of quality-of-service （QoS） multicast routing is to establish a multicast tree which satisfies certain constraints on bandwidth, delay and other metrics. The network state information maintained at every node is often im- precise in a dynamic environment because of non-negligible propagation delay of state messages, periodic updates due to overhead concern, and hierarchical state aggregation. The existing QoS multicast routing algorithms do not provide satisfactory performance with imprecise state information. We propose a distributed QoS multicast routing scheme based on traffic lights, called QMRI algorithm, which can probe multiple feasible tree branches, and select the optimal or near-optimal branch through the UR or TL mode for constructing a multicast tree with QoS guarantees if it exists. The scheme is designed to work with imprecise state information. The proposed algorithm considers not only the QoS requirements but also the cost optimality of the multicast tree. The correctness proof and the complexity analysis about the QMRI algorithm are also given. In addition, we develop NS2 so that it is able to simulate the imprecise network state information. Extensive simulations show that our algorithm achieves high call-admission ratio and low-cost multicast trees with modest message overhead.
文摘Y2O2S：Eu nano crystallines were prepared by a new ethanol assisted combustion synthesis method using sulfurcontained organic fuel in an ethanol-aqueous solution. The as-prepared nanocrystallines were characterized by X-ray diffraction, transmission electron microscope, photoluminescence spectra and X-ray luminescence spectra. It is shown that the assistant fuel ethanol has the effect of decreasing the water needed, simplifying the experiment procedure by dissolving rare earth nitrate and sulfur-contained organic fuel into an even solution, and prompting the formation of rare earth oxysulfide by igniting firstly during heating that leads to combustion decomposition reaction. Y2O2S ： Eu nano crystallines with strong photoluminescence and X-ray luminescence are obtained using thioacetamide as organic fuel. Mixtures of Y2O3 ： Eu and Y2O2S ： Eu are acquired using thiourea as fuel, and the content of Y2O2S ： Eu increases until reaches to about half of the Y2O3 ： Eu with the increasing amount of thiourea. Y2O2SO4 ： Eu emerges when S/Y = 6 and increases with increasing thiourea amount.
基金Supported by the National Science Foundation of China （No. 60873219）.
文摘In this paper, a distributed muting strategy based on simplified topology （DRBST） was proposed for LEO satellite networks. The topology of LEO satellite networks was simplified aiming at minimizing intersatellite links handover number. To optimize the route based on the simplified topology, we considered not only the transmission delay but also the queuing delay and the processing delay, which were analyzed using Markov chain and determined using a novel methodology. The DRBST algorithm was simulated in a LEO satellite networks model built using OPNET. The simulation results demonstrate that the low complexity DRBST algorithm can guarantee end-to-end delay bound. Moreover, the muting protocol cost is much less than traditional algorithms.
文摘Geographic routing has been introduced in mobile ad hoc networks and sensor networks. But its per-formance suffers greatly from mobility-induced location errors that can cause Lost Link （LLNK） and LOOP problems. Thus various mobility prediction algorithms have been proposed to mitigate the errors, but sometimes their prediction errors are substantial. A novel mobility prediction technique that incorpo-rates both mobile positioning information and road topology knowledge was presented. Furthermore, the performance of the scheme was evaluated via simulations, along with two other schemes, namely, Linear Velocity Prediction （LVP） and Weighted Velocity Prediction （WVP） for comparison purpose. The results of simulation under Manhattan mobility model show that the proposed scheme could track the movement of a node well and hence provide noticeable improvement over LVP and MVP.
基金Hunan Provincial Education Department of Key Projects(No.08A019)Funded Projects in Hunan Science and Technology Department(No.2008SK4029)
文摘The dynamical-wave routing model of the urban unsteady and non-pressure rain pipe flow was established by conservation of mass, momentum and energy, and it was solved by applying the four point implicit difference method and the pursuit method. It was obtained from the experiment checking and comparative analysis that the dynamical-wave muting model can reflect influence like attenuate and backwater when flood peak propagate in pipeline with high calculation precision and vast application scope, and it can be applied in routing of urban rain pipe flow of different slopes and inflow conditions. The routing model supplies a scientific foundation for the town rainfall piping design or checking, disaster administration of storm runoff, and so on.
基金supported by the Hi-Tech Research and Development Program of China(2006AA01Z238)the National Natural Science Foundation of China(90704006)+3 种基金the National BasicResearch Program of China(2007CB310705)PCSIRT(IRT0609)ISTCP(2006DFA11040)111 Project(B07005)
文摘The article proposes a network parameter-awareness （NPA） method and applies it to routing discovery algorithms in autonomic optical Internet. This NPA method can perceive the main parameters of the network, such as delay, jitter and traffic, which can represent the current situation of the network. And these parameters enable network to determine the appropriate nodes for routing discovery. The simulation results of evaluating performance of a network with NPA method and its routing applications show that the method and its applications in routing improve the performance of the network significantly with quality of service （QoS） guaranteed.
