Polypehnol is an important,potentially bioactive component of Sargassum muticum.In this study,ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38%ethanol solution at a solid:liquid ra...Polypehnol is an important,potentially bioactive component of Sargassum muticum.In this study,ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38%ethanol solution at a solid:liquid ratio of 1:30 at 68℃ for 32min,determined by single-factor and response surface methodology(RSM)optimization.The content of polyphenol was 5.66mg/g in the crude extract.Further extraction showed that the polyphenol mainly distributed in ethyl acetate(SKEE)and water phases(SKEW).The anti-oxidation test by electron spin resonance(ESR)spectrum showed that the SKEE had the strongest scavenging activity on DPPH(1,1-diphenyl-2-picrylhydrazyl)and alkyl radicals.SKEE was shown noncytotoxic but could inhibit the generation of cellular ROS,showing protective effects in H2O2 and AAPHinduced Vero cells and UV-B irradiated HaCaT cells.SKEE also signifi cantly inhibited the release of NO of LPS-induced RAW 264.7 cells.Therefore,the polyphenol-rich extracts in ethanol and ethyl acetate showed excellent anti-oxidant and anti-infl ammatory activities,which is beneficial to the development of high-value bio-substances.展开更多
Oceans and seas form a large body of water that contains a natural biodiversity.For humans,represents a resource,which makes this a point of interest,from researches to improve the economy.Seaweeds produce many compou...Oceans and seas form a large body of water that contains a natural biodiversity.For humans,represents a resource,which makes this a point of interest,from researches to improve the economy.Seaweeds produce many compounds and secondary metabolites that can be used in different fi elds of industry such as food,agricultural,pharmaceutical and health.Even though seaweeds are ancestral resources,recently it was notorious a global interest in knowing more about its potentials,where biotechnology plays an important role in research.Studies showed that seaweed has many bioactive compounds benefi cial to plant development,giving them a great potential as an agricultural fertilizer.Adding seaweeds to the soil provides organic matter,minerals,trace elements,growth plant regulator,metabolites,vitamins,and amino acids and it can work as a soil conditioner.In Portugal,the use of seaweeds for agriculture is important since long time ago.In the past,populations that lived near coastal zone depended on the seaweeds as a family subsistence but,throughout the years,synthetic fertilizers replaced seaweeds.Our work aimed to assess the potential of the extracts obtained from Ascophyllum nodosum and from Sargassum muticum as an agricultural fertilizer.This evaluation was carried out with rice plants(Oryza sativa)and lettuce(Lactuca sativa),in germination bioassays,the culture of rice and lettuce plants in pots,and culture of lettuce plants in hydroponics.For that,seaweed liquid extracts were used in different concentrations in different bioassays.Results show that extracts obtained from two seaweeds,A.nodosum and S.muticum,can be promissory plant biofertilizer at a concentration of 25% and had a positive effect on seed germination,plant development,and production.展开更多
Sargassum muticum is one of the most abundant and conspicuous native macroalgae species on the northern coasts of China.It often forms large-scale seaweed beds in subtidal zones.This investigation was designed to unde...Sargassum muticum is one of the most abundant and conspicuous native macroalgae species on the northern coasts of China.It often forms large-scale seaweed beds in subtidal zones.This investigation was designed to understand the intraspecific genetic relationships of this alga based on samples collected from four northern coastal sites of China,and to evaluate gamete release and growth capacity in laboratory conditions.The nuclear internal transcribed spacer 2(ITS2) sequences of 16 samples from four locations were identical.Based on cox3 gene and partial rbcLS operon sequences,intraspecific genetic variability was detected with three and two ribotypes,respectively.Temperature,not irradiance,was shown to significantly affect gamete release and fertilization.Elevated temperature and irradiance enhanced the growth of germlings and vegetative branchlets.Maximum growth rate of germlings was detected at 18–24°C and an irradiance of 60–100 μmol photons/(m2 s).Under ambient conditions(12–25°C and 60–125 μmol photons/(m2 s)),relative growth rate of young branchlets could reach 7.5%/d.展开更多
Objective:To evaluate the antibacterial efficiency of the seaweed,Sargassum muticum(S.muticum)collected from Pudumadam,Ramanathapuram,Tamil Nadu,India.Methods:Crude solvent extracts of S.muticum were obtained by using...Objective:To evaluate the antibacterial efficiency of the seaweed,Sargassum muticum(S.muticum)collected from Pudumadam,Ramanathapuram,Tamil Nadu,India.Methods:Crude solvent extracts of S.muticum were obtained by using Soxhlet extraction and the solvents like acetone,methanol and chloroform.These different extracts were tested against different human bacterial pathogens such as Micrococcus sp.,Staphylococcus aureus(methicillin resistance),Salmonella paratyphi B,Staphylococcus epidermis(3615),Enterobacter aerogenus(111),Klebsiella pneumonia(109),Shigella fleschneri(1457)(S.fleschneri),Proteus vulgaris(1771),Staphylococcus aureus(96)and Salmonella typhymurium(SP7)which were obtained from Microbial Type Culture Collection,Indian Institute of Microbial Technology,Chandigarh,India.Results:The results revealed that acetone extract had unveiled the maximum of 11 mm zone of inhibition at 40μL against S.fleschneri.Similar zone of inhibition(11 mm)was also observed at 50μL against Micrococcus sp.and S.fleschneri.Followed by acetone extract,chloroform extract also contributed 11 mm zone of inhibition against S.fleschneri and Salmonella paratyphi B at 40 and 50μL respectively.Besides,methanol extracts revealed meager antibacterial activity(9 mm).Conclusions:The present investigation suggests that the phytochemical constituent of the S.muticum might be suitable agents for the control of human deadly diseases.展开更多
基金Supported by the Public Science and Technology Research Funds Projects of Ocean(No.201505022)the Shandong Province Key Research and Development Project(No.2017YYSP003)the Natural Science Foundation of Shandong Province(No.ZR2017MD006)
文摘Polypehnol is an important,potentially bioactive component of Sargassum muticum.In this study,ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38%ethanol solution at a solid:liquid ratio of 1:30 at 68℃ for 32min,determined by single-factor and response surface methodology(RSM)optimization.The content of polyphenol was 5.66mg/g in the crude extract.Further extraction showed that the polyphenol mainly distributed in ethyl acetate(SKEE)and water phases(SKEW).The anti-oxidation test by electron spin resonance(ESR)spectrum showed that the SKEE had the strongest scavenging activity on DPPH(1,1-diphenyl-2-picrylhydrazyl)and alkyl radicals.SKEE was shown noncytotoxic but could inhibit the generation of cellular ROS,showing protective effects in H2O2 and AAPHinduced Vero cells and UV-B irradiated HaCaT cells.SKEE also signifi cantly inhibited the release of NO of LPS-induced RAW 264.7 cells.Therefore,the polyphenol-rich extracts in ethanol and ethyl acetate showed excellent anti-oxidant and anti-infl ammatory activities,which is beneficial to the development of high-value bio-substances.
基金the support of Fundacao para a Ciência e Tecnologia(FCT),through the strategic project UID/MAR/04292/2019 granted to MAREthe support from the European Union through EASME Blue Labs project AMALIA-Algaeto-MArket Lab IdeAs(EASME/EMFF/2016/1.2.1.4/03/SI2.750419)+2 种基金funding from European Structural&Investment Funds through the COMPETE ProgrammeNational Funds through FCT-Fundacao para a Ciência e a Tecnologia under the Programme grant SAICTPAC/0019/2015Co-financed by the European Regional Development Fund through the Interreg Atlantic Area Programme,under the project NASPA
文摘Oceans and seas form a large body of water that contains a natural biodiversity.For humans,represents a resource,which makes this a point of interest,from researches to improve the economy.Seaweeds produce many compounds and secondary metabolites that can be used in different fi elds of industry such as food,agricultural,pharmaceutical and health.Even though seaweeds are ancestral resources,recently it was notorious a global interest in knowing more about its potentials,where biotechnology plays an important role in research.Studies showed that seaweed has many bioactive compounds benefi cial to plant development,giving them a great potential as an agricultural fertilizer.Adding seaweeds to the soil provides organic matter,minerals,trace elements,growth plant regulator,metabolites,vitamins,and amino acids and it can work as a soil conditioner.In Portugal,the use of seaweeds for agriculture is important since long time ago.In the past,populations that lived near coastal zone depended on the seaweeds as a family subsistence but,throughout the years,synthetic fertilizers replaced seaweeds.Our work aimed to assess the potential of the extracts obtained from Ascophyllum nodosum and from Sargassum muticum as an agricultural fertilizer.This evaluation was carried out with rice plants(Oryza sativa)and lettuce(Lactuca sativa),in germination bioassays,the culture of rice and lettuce plants in pots,and culture of lettuce plants in hydroponics.For that,seaweed liquid extracts were used in different concentrations in different bioassays.Results show that extracts obtained from two seaweeds,A.nodosum and S.muticum,can be promissory plant biofertilizer at a concentration of 25% and had a positive effect on seed germination,plant development,and production.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A413)the National Natural Science Foundation of China(Nos.41206146,41176135)the Leading Academic Discipline Project of Shanghai Municipal Education Commission(No.J50701)
文摘Sargassum muticum is one of the most abundant and conspicuous native macroalgae species on the northern coasts of China.It often forms large-scale seaweed beds in subtidal zones.This investigation was designed to understand the intraspecific genetic relationships of this alga based on samples collected from four northern coastal sites of China,and to evaluate gamete release and growth capacity in laboratory conditions.The nuclear internal transcribed spacer 2(ITS2) sequences of 16 samples from four locations were identical.Based on cox3 gene and partial rbcLS operon sequences,intraspecific genetic variability was detected with three and two ribotypes,respectively.Temperature,not irradiance,was shown to significantly affect gamete release and fertilization.Elevated temperature and irradiance enhanced the growth of germlings and vegetative branchlets.Maximum growth rate of germlings was detected at 18–24°C and an irradiance of 60–100 μmol photons/(m2 s).Under ambient conditions(12–25°C and 60–125 μmol photons/(m2 s)),relative growth rate of young branchlets could reach 7.5%/d.
基金Supported by UGC-RGNF New Delhi(Ref.No:14–2(SC)/2007-SA-III).
文摘Objective:To evaluate the antibacterial efficiency of the seaweed,Sargassum muticum(S.muticum)collected from Pudumadam,Ramanathapuram,Tamil Nadu,India.Methods:Crude solvent extracts of S.muticum were obtained by using Soxhlet extraction and the solvents like acetone,methanol and chloroform.These different extracts were tested against different human bacterial pathogens such as Micrococcus sp.,Staphylococcus aureus(methicillin resistance),Salmonella paratyphi B,Staphylococcus epidermis(3615),Enterobacter aerogenus(111),Klebsiella pneumonia(109),Shigella fleschneri(1457)(S.fleschneri),Proteus vulgaris(1771),Staphylococcus aureus(96)and Salmonella typhymurium(SP7)which were obtained from Microbial Type Culture Collection,Indian Institute of Microbial Technology,Chandigarh,India.Results:The results revealed that acetone extract had unveiled the maximum of 11 mm zone of inhibition at 40μL against S.fleschneri.Similar zone of inhibition(11 mm)was also observed at 50μL against Micrococcus sp.and S.fleschneri.Followed by acetone extract,chloroform extract also contributed 11 mm zone of inhibition against S.fleschneri and Salmonella paratyphi B at 40 and 50μL respectively.Besides,methanol extracts revealed meager antibacterial activity(9 mm).Conclusions:The present investigation suggests that the phytochemical constituent of the S.muticum might be suitable agents for the control of human deadly diseases.
