In this paper, superhigh reproductive rate strains of MEV with titre more than HA8192* or TCID50 log9.7 10 have been achieved both by cultivation in cell lines with different susceptibility to MEV and by isolating and...In this paper, superhigh reproductive rate strains of MEV with titre more than HA8192* or TCID50 log9.7 10 have been achieved both by cultivation in cell lines with different susceptibility to MEV and by isolating and identifying in field by the author. The systematic tests proved that S18 and L12 strains of MEV are the best strains for vaccine preparation. In this study, the best means for the tissue cultivation of MEV and the most advanced technological process for the production and detection of serum-free cell-cultured MEV fluids with super-high HA titre in batches in large quantities have been established for the first time. Optimum conditions for MEV inactivation were determined, and safe and effective inactivated vaccines with mineral oil or A1(OH)3 gel adjuvant were successfully prepared with serum-free cell-cultured MEV fluids. Both vaccines with different adjuvants can be manufactured in batches in large quantities and have been widely used all over China since 1986. The change laws of the immune response kinetics of the MEV vaccines have been determined by the tests, and the indexes and laboratory animal models for monitoring humoral immune efficiency and immune protective rate of parvovirus vaccines have been established for the first time. The manufacturing and monitoring operation rules of the MEV vaccines with different adjuvants have been laid down by the author.展开更多
In the past two decades,many statistical depth functions seemed as powerful exploratoryand inferential tools for multivariate data analysis have been presented.In this paper,a new depthfunction family that meets four ...In the past two decades,many statistical depth functions seemed as powerful exploratoryand inferential tools for multivariate data analysis have been presented.In this paper,a new depthfunction family that meets four properties mentioned in Zuo and Serfling(2000)is proposed.Then aclassification rule based on the depth function family is proposed.The classification parameter b couldbe modified according to the type-Ⅰ error α,and the estimator of b has the consistency and achievesthe convergence rate n^(-1/2).With the help of the proper selection for depth family parameter c,theapproach for discriminant analysis could minimize the type-Ⅱ error β.A simulation study and a realdata example compare the performance of the different discriminant methods.展开更多
文摘In this paper, superhigh reproductive rate strains of MEV with titre more than HA8192* or TCID50 log9.7 10 have been achieved both by cultivation in cell lines with different susceptibility to MEV and by isolating and identifying in field by the author. The systematic tests proved that S18 and L12 strains of MEV are the best strains for vaccine preparation. In this study, the best means for the tissue cultivation of MEV and the most advanced technological process for the production and detection of serum-free cell-cultured MEV fluids with super-high HA titre in batches in large quantities have been established for the first time. Optimum conditions for MEV inactivation were determined, and safe and effective inactivated vaccines with mineral oil or A1(OH)3 gel adjuvant were successfully prepared with serum-free cell-cultured MEV fluids. Both vaccines with different adjuvants can be manufactured in batches in large quantities and have been widely used all over China since 1986. The change laws of the immune response kinetics of the MEV vaccines have been determined by the tests, and the indexes and laboratory animal models for monitoring humoral immune efficiency and immune protective rate of parvovirus vaccines have been established for the first time. The manufacturing and monitoring operation rules of the MEV vaccines with different adjuvants have been laid down by the author.
基金supported by the Natural Science Foundation of China under Grant Nos.10901020,10726013 and 10771017
文摘In the past two decades,many statistical depth functions seemed as powerful exploratoryand inferential tools for multivariate data analysis have been presented.In this paper,a new depthfunction family that meets four properties mentioned in Zuo and Serfling(2000)is proposed.Then aclassification rule based on the depth function family is proposed.The classification parameter b couldbe modified according to the type-Ⅰ error α,and the estimator of b has the consistency and achievesthe convergence rate n^(-1/2).With the help of the proper selection for depth family parameter c,theapproach for discriminant analysis could minimize the type-Ⅱ error β.A simulation study and a realdata example compare the performance of the different discriminant methods.
