“醒脑开窍”针法对实验性脑缺血大鼠心肌超微结构损伤的保护作用

采用阻断大鼠一侧 MCA造成局部脑缺血模型 ,观察脑缺血 2 4小时后 ,心肌超微结构的变化及针刺的影响。结果表明 :脑缺血后 ,心肌线粒体、肌原纤维、细胞核等发生明显损伤 (P<0 .0 1 ) ,并且毛细血管形态改变 ,“醒脑开窍”针法对心肌超微结构损伤具有明显改善作用 (P<0 .0 1 ) ,并优于传统针法组 (P<0 .0 5 ,P<0 .0 1 )。

急性心肌梗死后TGF-β1/Smad3通路的表达及活血化瘀中药的干预

目的:通过研究活血化瘀中药对TGF-β1/Smad3通路的影响,探讨活血化瘀法对急性心梗后心肌重塑的效应机制。方法清洁级Wistar大鼠50只随机分为5组,分别为正常组、模型组、假手术组、活血化瘀组、AMD3100组,每组10只。祛瘀生组大鼠术前3 d给药,1次/d。7 d后,实时荧光定量RT-PCR检测心肌组织转化生长因子(TGF)β1mRNA、smad3mRNA表达,分析各组非梗死区心肌胶原含量,并观察心肌组织H-E染色结果。结果:模型组心肌组织TGF-β1mRNA、smad3mRNA表达均增加(P<0.05);活血化瘀组能降低心肌组织TGF-β1mRNA、smad3mRNA表达水平,降低心肌胶原含量,并能减轻心肌组织的病理损伤;活血化瘀组与AMD3100组相比较TGF-β1mRNA、smad3mRNA表达无明显差异(P>0.05)。结论:活血化瘀法可能通过对TGF-β1/Smad3通路的抑制作用,从而有效的抑制急性心梗后心肌重塑。

3，4－二羟基苯乙酮对家兔缺血－再灌心肌的影响

以超微结构、线粒体形态计量及ＬＤＨ，ＳＯＤ活性为依据，探讨３，４－二羟基苯乙酮（ＤＨＡＰ）对家兔缺血－再灌心肌细胞的影响。结果表明：ＤＨＡＰ组缺血－再灌心肌细胞的损伤、ＬＤＨ丢失及ＳＯＤ活性下降的程度均明显低于未用药组。提示该药物对缺血－再灌心肌有保护作用。

尿毒素三肽和氢化可的松协同延长小鼠异位移植心肌的存活时间

目的：研究人工合成的尿毒素三肽（Ｈ－Ｈｉｓ－Ｇｌｙ－Ｌｙｓ－ＯＨ和Ｈ－Ｈｉｓ－Ｇｌｙ－Ｇｌｕ－ＯＨ）对异位移植心肌移植排斥的影响。方法：将Ｃ５７ＢＬ／６Ｊ新生乳鼠的半心移植到Ｂａｌｂ／ｃ成年小鼠耳后皮肤夹囊内，记录移植心肌电位的变化作为判别心肌存活的指标。结果：尿毒素三肽能显著延长异位移植心肌组织的存活时间。氢化可的松和尿毒肽的混合作用比单独给予尿毒素肽或氢化可的松作用强，并存在协同效应。结论：尿毒素三肽具有抑制小鼠异位移植心肌排斥的作用。氢化可的松和尿毒素三肽可协同延长异位移植心肌组织的存活时间。

Quantitative Assessment of Coronary Flow Reserve by the Variables of Time-Intensity Curve with Myocardial Contrast Echocardiography

The reliability and reliable indexes of q ua ntitative assessment of coronary flow reserve (CFR) by using time intensity cur ve (TIC) via myocardial contrast echocardiography were investigated. The TIC var iables were obtained by employing acoustic densitometry (AD) technique before an d after acetylcholine (Ach) injection in 12 dogs. Meanwhile, the correlation be tween these variables and CFR was analyzed. Among the variables derived from TIC , peak intensity (PI), area under the curve (AUC) and descending slope (DS) were increased significantly ( P <0.05) with the increase of coronary blood flow a fter Ach injection. Conversely, time to peak (TP), half time of descent (HT) , and mean transit time (MTT) were decreased remarkably ( P <0.0001). Th e P I and AUC ratios from post to pre Ach injection were strongly associated with CFR with the correlation coefficient (r) being 0.8366 and 0.8824, respectively. It is reliable by using the variables derived from TIC with myocardial contrast echocardiography to quantitatively evaluate regional myocardial CFR. The PI an d AUC ratios from post to pre Ach injection are the reliable indexes for quan titative assessment of CFR.

缺血再灌注对未成熟兔心冠脉微循环结构和功能损伤的研究

在离体做功模型上观察单低温１０～１５℃与低温加ＴｈｏｍａｓⅡ号心停搏液单剂和多剂灌注对３周龄兔全心缺血再灌注的作用。结果发现单剂组较低温组和多剂组心肌线粒体丙二醛，心肌和冠脉引流液内皮素含量降低，冠脉引流液一氧化氮含量增加，再灌流微血管开放良好，内皮细胞超微结构损伤减轻，心输出量恢复优于二组。多剂组与低温组组上述改变相似。提示局部低温加Ｔｈｏｍａｓ液单剂灌注对未成熟兔心冠脉微循环结构和功能的保护作用优于单低温，而多剂组则未见增加。