Orthophosphate is an essential but limiting macronutrient for plant growth. About 67% cropland in China lacks sufficient phosphorus, especially that with red soil. Extensive soil phosphorus reserves exist in the form ...Orthophosphate is an essential but limiting macronutrient for plant growth. About 67% cropland in China lacks sufficient phosphorus, especially that with red soil. Extensive soil phosphorus reserves exist in the form of organic phosphorus, which is unavailable for root uptake unless hydrolyzed by secretory acid phosphatases. Thus, many microorganisms with the ability to produce phosphatase have been exploited. In this work, the activity of an extracellular acid phosphatase and yeast biomass from Candida mycoderma was measured under different culture conditions, such as pH, temperature, and carbon source. A maximal phosphatase activity of 8.47 × 10^5±0.11× 10^5 U/g was achieved by C. Mycoderma in 36 hr under the optimal conditions. The extracellular acid phosphatase has high activity over a wide pH tolerance range from 2.5 to S.0 （optimum pH 3.5）. The effects of different phosphorus compounds on the acid phosphatase production were also studied. The presence of phytin, lecithin or calcium phosphate reduced the phosphatase activity and biomass yield significantly. In addition, the pH of the culture medium was reduced significantly by lecithin. The efficiency of the strain in releasing orthophosphate from organic phosphorus was studied in red soil （used in planting trees） and rice soil （originating as red soil）. The available phosphorus content was increased by 230% after inoculating 20 days in rice soil and decreased by 50% after inoculating 10 days in red soil. This work indicates that the yeast strain C. mycoderma has potential application for enhancing phosphorus utilization in plants that grow in rice soil.展开更多
目的验证复方氟康唑凝胶对耐氟康唑假丝酵母菌的抗菌效果。方法选用琼脂扩散法,比较复方氟康唑凝胶与氟康唑凝胶对耐氟康唑假丝酵母菌的体外抑菌效果;通过感染耐氟康唑假丝酵母菌大鼠治疗试验,比较复方氟康唑凝胶与氟康唑凝胶治疗效果...目的验证复方氟康唑凝胶对耐氟康唑假丝酵母菌的抗菌效果。方法选用琼脂扩散法,比较复方氟康唑凝胶与氟康唑凝胶对耐氟康唑假丝酵母菌的体外抑菌效果;通过感染耐氟康唑假丝酵母菌大鼠治疗试验,比较复方氟康唑凝胶与氟康唑凝胶治疗效果。结果复方氟康唑凝胶抑菌圈直径(18.55±0.03) mm (n=20),氟康唑凝胶抑菌圈直径(7.12±0.01) mm (n=20),差异具有统计学意义(P<0.05);复方氟康唑凝胶治疗感染耐氟康唑假丝酵母菌大鼠15 d的转阴率为80%,氟康唑凝胶为40%,差异具有统计学意义(P<0.05,n=15);复方氟康唑凝胶抑制阴道灌洗液炎性细胞因子水平能力、减轻病变损伤程度明显高于氟康唑凝胶(P<0.05,n=15)。结论复方氟康唑凝胶对耐氟康唑假丝酵母菌的抗菌效果肯定,较氟康唑凝胶具有优势。展开更多
基金supported by a grant from the Special Project for Forest Public Benefit (No. 200904055)Project for Advantage Life Science of Jiangshu Province and Open Project of Jiangsu Key Laboratory for Biodiversity and Biotechnology (No. 164070302115)
文摘Orthophosphate is an essential but limiting macronutrient for plant growth. About 67% cropland in China lacks sufficient phosphorus, especially that with red soil. Extensive soil phosphorus reserves exist in the form of organic phosphorus, which is unavailable for root uptake unless hydrolyzed by secretory acid phosphatases. Thus, many microorganisms with the ability to produce phosphatase have been exploited. In this work, the activity of an extracellular acid phosphatase and yeast biomass from Candida mycoderma was measured under different culture conditions, such as pH, temperature, and carbon source. A maximal phosphatase activity of 8.47 × 10^5±0.11× 10^5 U/g was achieved by C. Mycoderma in 36 hr under the optimal conditions. The extracellular acid phosphatase has high activity over a wide pH tolerance range from 2.5 to S.0 （optimum pH 3.5）. The effects of different phosphorus compounds on the acid phosphatase production were also studied. The presence of phytin, lecithin or calcium phosphate reduced the phosphatase activity and biomass yield significantly. In addition, the pH of the culture medium was reduced significantly by lecithin. The efficiency of the strain in releasing orthophosphate from organic phosphorus was studied in red soil （used in planting trees） and rice soil （originating as red soil）. The available phosphorus content was increased by 230% after inoculating 20 days in rice soil and decreased by 50% after inoculating 10 days in red soil. This work indicates that the yeast strain C. mycoderma has potential application for enhancing phosphorus utilization in plants that grow in rice soil.
文摘目的验证复方氟康唑凝胶对耐氟康唑假丝酵母菌的抗菌效果。方法选用琼脂扩散法,比较复方氟康唑凝胶与氟康唑凝胶对耐氟康唑假丝酵母菌的体外抑菌效果;通过感染耐氟康唑假丝酵母菌大鼠治疗试验,比较复方氟康唑凝胶与氟康唑凝胶治疗效果。结果复方氟康唑凝胶抑菌圈直径(18.55±0.03) mm (n=20),氟康唑凝胶抑菌圈直径(7.12±0.01) mm (n=20),差异具有统计学意义(P<0.05);复方氟康唑凝胶治疗感染耐氟康唑假丝酵母菌大鼠15 d的转阴率为80%,氟康唑凝胶为40%,差异具有统计学意义(P<0.05,n=15);复方氟康唑凝胶抑制阴道灌洗液炎性细胞因子水平能力、减轻病变损伤程度明显高于氟康唑凝胶(P<0.05,n=15)。结论复方氟康唑凝胶对耐氟康唑假丝酵母菌的抗菌效果肯定,较氟康唑凝胶具有优势。
