Objective： To investigate the effect of berberine, one of the main alkaloids of Rhizoma coptidis, on myocardial morphology and the expressions of cyclin-dependent kinase 9 （Cdk9） and cyclin T1 protein in the myocar...Objective： To investigate the effect of berberine, one of the main alkaloids of Rhizoma coptidis, on myocardial morphology and the expressions of cyclin-dependent kinase 9 （Cdk9） and cyclin T1 protein in the myocardium of type 2 diabetic rats. Methods： Type 2 diabetes mellitus rats were induced by an injection of 35 mg/kg streptozotocin （STZ） and a high-carbohydrate/high-fat diet for 16 weeks. Diabetic rats were given low-, middle-, high-dose berberine （75, 150, 300 mg/kg）, fenofibrate （100 mg/kg） and rosiglitazone （4 mg/kg） for another 16 weeks, respectively. The myocardium structure was observed with hematoxylin ＆ eosin （H＆E） staining and Cdk9 and cyclin T1 protein expressions were detected by immunohistochemistry. Results： Middle-dose, high-dose berberine improved myocardial hypertrophy and interstitial fibrosis of diabetic rats. Cdk9 and cyclin T1 protein were significantly lower in diabetic myocardium than in control one （P〈0.01）, and middle-dose, high-dose berberine and fenofibrate obviously increased both Cdk9 and cyclin T1 expression to near control level （P〈0.01）. Conclusion： Berberine modulates Cdk9 and cyclin T1 protein expression in diabetic myocardium which may contribute to ameliorate myocardium damage.展开更多
Objective To investigate the effect of acute myocardium ischemic on heart function of pregnancy rat. Methods 13 female SD rats and 6 early pregnancy rats were divided into normal group, unpregnant group with acute myo...Objective To investigate the effect of acute myocardium ischemic on heart function of pregnancy rat. Methods 13 female SD rats and 6 early pregnancy rats were divided into normal group, unpregnant group with acute myocardial infarction and early pregnant group with acute myocardial infarction. The anterior branch of the left coronary artery was ligated. 3 weeks later, Image 1.31 software was used to measure areas of myocardial infarction, and to evaluate hemodynamics of heart with powerLAB4.12, and cardiac tissues were stained with Massion. Results Compared with unpregnant group with acute myocardial infarction , the early pregnant group with acute myocardial infarction had less myocardial infarction area (28.86% vs. 36.8%),and had a higher left ventricle end systolic pressure, ±dp/dt _ max , and lower left ventricle end diastolic pressure. Massion stain showed the amount of collagen of the lesion was less in the early pregnant group with acute myocardial infarction than that in unpregnant group. Conclusion The early pregnant group with acute myocardial infarction had better heart contractive and diastolic function .展开更多
Novel hexadecyl 3-{4-[2-hydroxy-3(isopropylamino)propoxy]phenyl}propionate(HPP)was synthesized and its effect on delivery of liposomes into cultured cardiomyocytes was examined.The structure of HPP was characterized b...Novel hexadecyl 3-{4-[2-hydroxy-3(isopropylamino)propoxy]phenyl}propionate(HPP)was synthesized and its effect on delivery of liposomes into cultured cardiomyocytes was examined.The structure of HPP was characterized by IH NMR,1R and MS.The amount of cardiomyocytes uptake of HPP-liposome was 3.9-fold higher than plain-liposome,and the increase was 6.2-fold when hypoxia happens.It indicated that HPP was a potential ligand for liposome targeting to ischemic myocardium.展开更多
目的探讨胱抑素C(Cys-C)、餐后2小时C肽(2 h CP)、尿微量白蛋白/尿肌酐(UACR)、病程与2型糖尿病周围神经病变(DPN)的相关性。方法根据双下肢肌电图及足部定量感觉检查结果,选取庆阳市人民医院2021年1月至2022年1月的DPN患者100例作为观...目的探讨胱抑素C(Cys-C)、餐后2小时C肽(2 h CP)、尿微量白蛋白/尿肌酐(UACR)、病程与2型糖尿病周围神经病变(DPN)的相关性。方法根据双下肢肌电图及足部定量感觉检查结果,选取庆阳市人民医院2021年1月至2022年1月的DPN患者100例作为观察组、2型糖尿病非周围神经病变(NDPN)患者73例作为对照组,收集患者的实验室数据及临床指标,分析Cys-C、2 h CP、UACR、病程等指标与DPN之间的相关性。结果DPN组空腹胰岛素(FINS)、尿免疫球蛋白G、尿肌酐、病程、Cys-C、UACR均显著高于NDPN组,差异有统计学意义(t=-2.728,-3.826,-2114,-2.632,-2.850,-2.717,P<0.05);2 h CP水平显著低于NDPN组,差异有统计学意义(t=2.696,P<0.05);Logisitc回归显示,糖尿病病程、2 h CP、Cys-C与DPN具有相关性,差异有统计学意义(OR=1.863,0.729,3.799,P<0.05);ROC曲线显示,病程、Cys-C、2 h CP、糖化血红蛋白(HbA1c)四项联合的诊断性能较好,其次是Cys-C。结论Cys-C、2 h CP、UACR、病程与DPN相关,可能是疾病发作的危险性因素,临床需对此引起高度重视。展开更多
While several previous studies have indicated the link between periodontal disease (PD) and myocardial infarction (MI), theunderlying mechanisms remain unclear. Autophagy, a cellular quality control process that is ac...While several previous studies have indicated the link between periodontal disease (PD) and myocardial infarction (MI), theunderlying mechanisms remain unclear. Autophagy, a cellular quality control process that is activated in several diseases, includingheart failure, can be suppressed by Porphyromonas gingivalis (P.g.). However, it is uncertain whether autophagy impairment byperiodontal pathogens stimulates the development of cardiac dysfunction after MI. Thus, this study aimed to investigate therelationship between PD and the development of MI while focusing on the role of autophagy. Neonatal rat cardiomyocytes(NRCMs) and MI model mice were inoculated with wild-type P.g. or gingipain-deficient P.g. to assess the effect of autophagyinhibition by P.g. Wild-type P.g.-inoculated NRCMs had lower cell viability than those inoculated with gingipain-deficient P.g. Thisstudy also revealed that gingipains can cleave vesicle-associated membrane protein 8 (VAMP8), a protein involved in lysosomalsensitive factor attachment protein receptors (SNAREs), at the 47th lysine residue, thereby inhibiting autophagy. Wild-type P.g.-inoculated MI model mice were more susceptible to cardiac rupture, with lower survival rates and autophagy activity thangingipain-deficient P.g.-inoculated MI model mice. After inoculating genetically modified MI model mice (VAMP8-K47A) with wildtype P.g., they exhibited significantly increased autophagy activation compared with the MI model mice inoculated with wild-typeP.g., which suppressed cardiac rupture and enhanced overall survival rates. These findings suggest that gingipains, which arevirulence factors of P.g., impair the infarcted myocardium by cleaving VAMP8 and disrupting autophagy. This study confirms thestrong association between PD and MI and provides new insights into the potential role of autophagy in this relationship.展开更多
Objective Congestive heart failure （CHF） is the final common pathway of various heart diseases.Calcineurin,a calcium/calmodulindependent phosphatase consisting of a catalytic subanit A （CnA） and a regulatory calci...Objective Congestive heart failure （CHF） is the final common pathway of various heart diseases.Calcineurin,a calcium/calmodulindependent phosphatase consisting of a catalytic subanit A （CnA） and a regulatory calcium-binding subunit B （CnB）,is activated in heart failure.This study aimed to investigate the relationship between mRNA level of calcineurin in circulating T-lymphocyte and that in myocardium in patients with CHF. Methods A total of 38 patients with CHF （aged from 29 to 62 years） were included in this study.The mRNA levels of alpha-and beta-isoform of CnA in left ventricular anterior papillary muscle and peripheral lymphocytes were determined by semi-quantitative reverse transcription polymerase chain reaction.Pearson linear correlation analysis was performed,and difference was considered statistically significant at a P value 〈0.05. Results Calcineurin mRNA levels in lymphocytes were positively correlated with those in myocardium （for CnA-alpha mRNA,r=0.820;for CnA-beta mRNA,r=0.875;both P〈0.01）.CnA-beta mRNA levels in both circulating lymphocytes and myocardium increased significantly with increasing NYHA class （r=0.877 for peripheral blood and r=0.805 for cardiac muscle;both P〈0.01）. Conclusions The mRNA level of CnA-beta in circulating lymphocytes is positively correlated with that in myocardium and is a promising marker for the severity of cardiac dysfunction in patients with CHF.展开更多
To investigate the monophasic action potential changes of myocardiuminduced by radiofrequency catheter ablation in dogs,the right ventricularepicardium were ablated with radiofrequency(RF)energy(20 w,30 sac)via a 7 Fr...To investigate the monophasic action potential changes of myocardiuminduced by radiofrequency catheter ablation in dogs,the right ventricularepicardium were ablated with radiofrequency(RF)energy(20 w,30 sac)via a 7 Fr.large tip catheter in 8 dogs.Franz catheter was used to record at8 different sites and at 3 different time before and after ablation.Myocsrdial APA,APD90 and Vmax at the ablation site,marginal siteand reference site(20 mm from the ablation site)were measured beforeand after(immediately,30 min)ablation.The interval from stimulationspike(S)(stimulated at right ventricular apex)to the [0] phase of localMAP were also maasured.Results:1.Two to 10 mm from the ablationsite,APA decreased to different degree.There was no change in the APDafter RFCA.2.Vmax of ablation site and marginal zone decreased afterablation(P【0.01);30 min later,Vmax show no change compared with theresult immdietely after ablation(P】0.05).3.S-[0] phase interval increasedfrom ablation site to marginal site after ablation(ablation site 22.50±6.55ms vs 35.63±7.76 ms,P【0.001;marginal site 23.75±7.91 ms vs31.88+9.61 ms,P【0.01).Conclusion:RF energy can destroy or injuremyocardial tissue,and modificate cellular electrophysiology ofperinecrosis myocardium.These findings provide electrophyiologic basisfor sevasal clinincal observations following RFCA. Background:Most AV node reentrant tachycardia are easily induced duringelectrophysiologic studies.However,some could not be induced despite long timestimulation.How to deal with such patients properly is practically important.In thissituation,the radiofrequency ablation may provide a good results,but the experience islimited.The clinical effect need further investigation.Objective:To assess the clinicalefficacy of slow pathway ablation in patients with clinical documented but noninducibleAV node reentrant tachycardia.Methods:The criteria of presumed diagnosis of common-type AV node reentrant tachycardia included tachycardia with pseudo-r’ in lead VI,retrograde p wave on the end of QRS,or without retrograde p wave in all leads duringtachycardia,and/or presence of discontinous curve during the baseline electrophysiologicstudy.The stimuli technique included single,double extrastimuli at 2 basic derive cyclelengths and decremental pacing from atrium,ventricle and coronary sinus.If thetachycardia could not be induced,isoproterenol(1 to 8 ug/min)was used,If thetachycardia could not be initiated with isoproterenol,atropine(1 mg)was given 20 minutesafter termination of isoproterenol infusion.The sites of slow pathway ablation wereseptum or around ostium of coronary sinus with a small fragment A wave and large Vwave.The slow junctional rythms which decreased progressly or dispeared were goodindicator for successful ablation.The endpoints of ablation were elimination of slowpathway and /or significant alternation of Wanckebach point and ERP of AVconduction.The patiants were followed up after procedures.Results:Six patients(4female,2 male)were identified as noninducible AV node reentrant tachycardia.Theaverage age were 53±10 years and the history of tachycardia were 14.8±8.8 years.The heart rates of tachycardia were 180±10 beats/min.Four cases had ECGs oftachycardia,three had retrogrede P wave on the end of QRS wave and pesudo-r’ in VIlead.The remaining one had no retrograde p wave in all leads,The VA retrogradeWenckebach block were seen on 6 cases.Four had jamp of AV conduction(average 86±62 ms).After ablation,the jamp dispeared in three of them,one still had jamp withetrial echo.The ERP and Wenckebach points of AV or VA conduction were changed.During ablation,the junctional rythms were seen in 6 cases,decreased progressly in onecase and dispeared at last in five cases.None of six patients had recurrence of tachycardiaduring follow-up of 18±8.4 months.Conclusion:In patients with documented butnoninducible AV node reentrant tachycardia,the modification of slow pathway canachieve a satisfactory clinical effect.展开更多
文摘Objective： To investigate the effect of berberine, one of the main alkaloids of Rhizoma coptidis, on myocardial morphology and the expressions of cyclin-dependent kinase 9 （Cdk9） and cyclin T1 protein in the myocardium of type 2 diabetic rats. Methods： Type 2 diabetes mellitus rats were induced by an injection of 35 mg/kg streptozotocin （STZ） and a high-carbohydrate/high-fat diet for 16 weeks. Diabetic rats were given low-, middle-, high-dose berberine （75, 150, 300 mg/kg）, fenofibrate （100 mg/kg） and rosiglitazone （4 mg/kg） for another 16 weeks, respectively. The myocardium structure was observed with hematoxylin ＆ eosin （H＆E） staining and Cdk9 and cyclin T1 protein expressions were detected by immunohistochemistry. Results： Middle-dose, high-dose berberine improved myocardial hypertrophy and interstitial fibrosis of diabetic rats. Cdk9 and cyclin T1 protein were significantly lower in diabetic myocardium than in control one （P〈0.01）, and middle-dose, high-dose berberine and fenofibrate obviously increased both Cdk9 and cyclin T1 expression to near control level （P〈0.01）. Conclusion： Berberine modulates Cdk9 and cyclin T1 protein expression in diabetic myocardium which may contribute to ameliorate myocardium damage.
文摘Objective To investigate the effect of acute myocardium ischemic on heart function of pregnancy rat. Methods 13 female SD rats and 6 early pregnancy rats were divided into normal group, unpregnant group with acute myocardial infarction and early pregnant group with acute myocardial infarction. The anterior branch of the left coronary artery was ligated. 3 weeks later, Image 1.31 software was used to measure areas of myocardial infarction, and to evaluate hemodynamics of heart with powerLAB4.12, and cardiac tissues were stained with Massion. Results Compared with unpregnant group with acute myocardial infarction , the early pregnant group with acute myocardial infarction had less myocardial infarction area (28.86% vs. 36.8%),and had a higher left ventricle end systolic pressure, ±dp/dt _ max , and lower left ventricle end diastolic pressure. Massion stain showed the amount of collagen of the lesion was less in the early pregnant group with acute myocardial infarction than that in unpregnant group. Conclusion The early pregnant group with acute myocardial infarction had better heart contractive and diastolic function .
基金This project was supported by the National Natural Science Foundation of China(No.30271548).
文摘Novel hexadecyl 3-{4-[2-hydroxy-3(isopropylamino)propoxy]phenyl}propionate(HPP)was synthesized and its effect on delivery of liposomes into cultured cardiomyocytes was examined.The structure of HPP was characterized by IH NMR,1R and MS.The amount of cardiomyocytes uptake of HPP-liposome was 3.9-fold higher than plain-liposome,and the increase was 6.2-fold when hypoxia happens.It indicated that HPP was a potential ligand for liposome targeting to ischemic myocardium.
文摘目的探讨胱抑素C(Cys-C)、餐后2小时C肽(2 h CP)、尿微量白蛋白/尿肌酐(UACR)、病程与2型糖尿病周围神经病变(DPN)的相关性。方法根据双下肢肌电图及足部定量感觉检查结果,选取庆阳市人民医院2021年1月至2022年1月的DPN患者100例作为观察组、2型糖尿病非周围神经病变(NDPN)患者73例作为对照组,收集患者的实验室数据及临床指标,分析Cys-C、2 h CP、UACR、病程等指标与DPN之间的相关性。结果DPN组空腹胰岛素(FINS)、尿免疫球蛋白G、尿肌酐、病程、Cys-C、UACR均显著高于NDPN组,差异有统计学意义(t=-2.728,-3.826,-2114,-2.632,-2.850,-2.717,P<0.05);2 h CP水平显著低于NDPN组,差异有统计学意义(t=2.696,P<0.05);Logisitc回归显示,糖尿病病程、2 h CP、Cys-C与DPN具有相关性,差异有统计学意义(OR=1.863,0.729,3.799,P<0.05);ROC曲线显示,病程、Cys-C、2 h CP、糖化血红蛋白(HbA1c)四项联合的诊断性能较好,其次是Cys-C。结论Cys-C、2 h CP、UACR、病程与DPN相关,可能是疾病发作的危险性因素,临床需对此引起高度重视。
基金supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aid for Scientific Research (C) 20K08399 (to Yasuhiro Maejima)KAKENHI 19K18985,Grant-in-Aid for JSPS Fellows+1 种基金MSD Life Science FoundationPublic Interest Incorporated Foundation (to Yuka Shiheido-Watanabe)
文摘While several previous studies have indicated the link between periodontal disease (PD) and myocardial infarction (MI), theunderlying mechanisms remain unclear. Autophagy, a cellular quality control process that is activated in several diseases, includingheart failure, can be suppressed by Porphyromonas gingivalis (P.g.). However, it is uncertain whether autophagy impairment byperiodontal pathogens stimulates the development of cardiac dysfunction after MI. Thus, this study aimed to investigate therelationship between PD and the development of MI while focusing on the role of autophagy. Neonatal rat cardiomyocytes(NRCMs) and MI model mice were inoculated with wild-type P.g. or gingipain-deficient P.g. to assess the effect of autophagyinhibition by P.g. Wild-type P.g.-inoculated NRCMs had lower cell viability than those inoculated with gingipain-deficient P.g. Thisstudy also revealed that gingipains can cleave vesicle-associated membrane protein 8 (VAMP8), a protein involved in lysosomalsensitive factor attachment protein receptors (SNAREs), at the 47th lysine residue, thereby inhibiting autophagy. Wild-type P.g.-inoculated MI model mice were more susceptible to cardiac rupture, with lower survival rates and autophagy activity thangingipain-deficient P.g.-inoculated MI model mice. After inoculating genetically modified MI model mice (VAMP8-K47A) with wildtype P.g., they exhibited significantly increased autophagy activation compared with the MI model mice inoculated with wild-typeP.g., which suppressed cardiac rupture and enhanced overall survival rates. These findings suggest that gingipains, which arevirulence factors of P.g., impair the infarcted myocardium by cleaving VAMP8 and disrupting autophagy. This study confirms thestrong association between PD and MI and provides new insights into the potential role of autophagy in this relationship.
文摘Objective Congestive heart failure （CHF） is the final common pathway of various heart diseases.Calcineurin,a calcium/calmodulindependent phosphatase consisting of a catalytic subanit A （CnA） and a regulatory calcium-binding subunit B （CnB）,is activated in heart failure.This study aimed to investigate the relationship between mRNA level of calcineurin in circulating T-lymphocyte and that in myocardium in patients with CHF. Methods A total of 38 patients with CHF （aged from 29 to 62 years） were included in this study.The mRNA levels of alpha-and beta-isoform of CnA in left ventricular anterior papillary muscle and peripheral lymphocytes were determined by semi-quantitative reverse transcription polymerase chain reaction.Pearson linear correlation analysis was performed,and difference was considered statistically significant at a P value 〈0.05. Results Calcineurin mRNA levels in lymphocytes were positively correlated with those in myocardium （for CnA-alpha mRNA,r=0.820;for CnA-beta mRNA,r=0.875;both P〈0.01）.CnA-beta mRNA levels in both circulating lymphocytes and myocardium increased significantly with increasing NYHA class （r=0.877 for peripheral blood and r=0.805 for cardiac muscle;both P〈0.01）. Conclusions The mRNA level of CnA-beta in circulating lymphocytes is positively correlated with that in myocardium and is a promising marker for the severity of cardiac dysfunction in patients with CHF.
文摘To investigate the monophasic action potential changes of myocardiuminduced by radiofrequency catheter ablation in dogs,the right ventricularepicardium were ablated with radiofrequency(RF)energy(20 w,30 sac)via a 7 Fr.large tip catheter in 8 dogs.Franz catheter was used to record at8 different sites and at 3 different time before and after ablation.Myocsrdial APA,APD90 and Vmax at the ablation site,marginal siteand reference site(20 mm from the ablation site)were measured beforeand after(immediately,30 min)ablation.The interval from stimulationspike(S)(stimulated at right ventricular apex)to the [0] phase of localMAP were also maasured.Results:1.Two to 10 mm from the ablationsite,APA decreased to different degree.There was no change in the APDafter RFCA.2.Vmax of ablation site and marginal zone decreased afterablation(P【0.01);30 min later,Vmax show no change compared with theresult immdietely after ablation(P】0.05).3.S-[0] phase interval increasedfrom ablation site to marginal site after ablation(ablation site 22.50±6.55ms vs 35.63±7.76 ms,P【0.001;marginal site 23.75±7.91 ms vs31.88+9.61 ms,P【0.01).Conclusion:RF energy can destroy or injuremyocardial tissue,and modificate cellular electrophysiology ofperinecrosis myocardium.These findings provide electrophyiologic basisfor sevasal clinincal observations following RFCA. Background:Most AV node reentrant tachycardia are easily induced duringelectrophysiologic studies.However,some could not be induced despite long timestimulation.How to deal with such patients properly is practically important.In thissituation,the radiofrequency ablation may provide a good results,but the experience islimited.The clinical effect need further investigation.Objective:To assess the clinicalefficacy of slow pathway ablation in patients with clinical documented but noninducibleAV node reentrant tachycardia.Methods:The criteria of presumed diagnosis of common-type AV node reentrant tachycardia included tachycardia with pseudo-r’ in lead VI,retrograde p wave on the end of QRS,or without retrograde p wave in all leads duringtachycardia,and/or presence of discontinous curve during the baseline electrophysiologicstudy.The stimuli technique included single,double extrastimuli at 2 basic derive cyclelengths and decremental pacing from atrium,ventricle and coronary sinus.If thetachycardia could not be induced,isoproterenol(1 to 8 ug/min)was used,If thetachycardia could not be initiated with isoproterenol,atropine(1 mg)was given 20 minutesafter termination of isoproterenol infusion.The sites of slow pathway ablation wereseptum or around ostium of coronary sinus with a small fragment A wave and large Vwave.The slow junctional rythms which decreased progressly or dispeared were goodindicator for successful ablation.The endpoints of ablation were elimination of slowpathway and /or significant alternation of Wanckebach point and ERP of AVconduction.The patiants were followed up after procedures.Results:Six patients(4female,2 male)were identified as noninducible AV node reentrant tachycardia.Theaverage age were 53±10 years and the history of tachycardia were 14.8±8.8 years.The heart rates of tachycardia were 180±10 beats/min.Four cases had ECGs oftachycardia,three had retrogrede P wave on the end of QRS wave and pesudo-r’ in VIlead.The remaining one had no retrograde p wave in all leads,The VA retrogradeWenckebach block were seen on 6 cases.Four had jamp of AV conduction(average 86±62 ms).After ablation,the jamp dispeared in three of them,one still had jamp withetrial echo.The ERP and Wenckebach points of AV or VA conduction were changed.During ablation,the junctional rythms were seen in 6 cases,decreased progressly in onecase and dispeared at last in five cases.None of six patients had recurrence of tachycardiaduring follow-up of 18±8.4 months.Conclusion:In patients with documented butnoninducible AV node reentrant tachycardia,the modification of slow pathway canachieve a satisfactory clinical effect.
