A photo-induced de-oxygenation process of myoglobin (Mb) in aqueous solution was investigated by use of fluorescence spectroscopy. The spectra are characterized by the fluorescence intensity declining gradually after ...A photo-induced de-oxygenation process of myoglobin (Mb) in aqueous solution was investigated by use of fluorescence spectroscopy. The spectra are characterized by the fluorescence intensity declining gradually after each scan,and the decay of fluorescence intensity being significant in each scan,which is assigned to the release of oxygen from the opening of the heme-pockets induced by illumination. More illumination will cause more release of oxygen; if the temperature of an Mb solution is increased when it is illuminated,the rate of de-oxygenation will be higher. It was found that ligand-oxygen in Fe-porphyrin could be removed from Mb by nitrogen. This indicates that the interac-tion between oxy-Mb and other different gases can be tested by the method of fluorescence spectros-copy. In addition,fluorescence spectroscopy can be employed to probe the energy transfer between Fe-porphyrin and tryptophan or tyrosine in Mb molecules.展开更多
基金the National Natural Science Foundation of China (Grant No. 2021010)Program for Liaoning Excellent Talents in University (Grant No. RC-04-10)
文摘A photo-induced de-oxygenation process of myoglobin (Mb) in aqueous solution was investigated by use of fluorescence spectroscopy. The spectra are characterized by the fluorescence intensity declining gradually after each scan,and the decay of fluorescence intensity being significant in each scan,which is assigned to the release of oxygen from the opening of the heme-pockets induced by illumination. More illumination will cause more release of oxygen; if the temperature of an Mb solution is increased when it is illuminated,the rate of de-oxygenation will be higher. It was found that ligand-oxygen in Fe-porphyrin could be removed from Mb by nitrogen. This indicates that the interac-tion between oxy-Mb and other different gases can be tested by the method of fluorescence spectros-copy. In addition,fluorescence spectroscopy can be employed to probe the energy transfer between Fe-porphyrin and tryptophan or tyrosine in Mb molecules.
文摘以粒径约为120 nm的Fe_3O_4磁性纳米球为内核,经过SiO_2包埋,BSA修饰并吸附金纳米球三步后得到一种组装型金磁复合纳米球MNP@SiO_2@BSA@AuNPs,并以此纳米球为载体,建立了检测心梗标志物之一的肌红蛋白的新方法.金磁复合纳米球偶联肌红蛋白抗体4E2后,可快速分离和富集样品中的肌红蛋白,加入偶联有肌红蛋白抗体7C3的荧光硅纳米球后形成三明治夹心结构,通过检测荧光强度实现了对肌红蛋白的定量检测.结果表明:该检测方法在肌红蛋白浓度为0~250 ng·m L^(-1)的范围内荧光强度与肌红蛋白浓度具有良好的线性关系(R^2=0.993),选择性高,重复性好,快速检测肌红蛋白时间不超过30 min.
