Different n-6/n-3 polyunsaturated fatty acid ratios affect postprandial metabolism in normal and hypertriglyceridemic rats

Postprandial metabolism plays major roles in many pathological conditions.The n-6/n-3 polyunsaturated fatty acid(PUFA)ratio is closely related to various physiological disorders.This study aimed to investigate the effects of high fat meals with different n-6/n-3 PUFA ratios on postprandial metabolism in normal control(NC)and hypertriglyceridemia(HTG)rats.The postprandial response of triglyceride(TG)in HTG groups was higher than that in NC groups after different n-6/n-3 PUFA ratio meals.The HTG groups showed higher postprandial total cholesterol(TC)responses than NC groups after 1:1 and 20:1 ratio meals.The 5:1 n-6/n-3 PUFA ratio elicited lower postprandial responses of tumor necrosis factorα(TNF-α)than 1:1 and 10:1 ratios in HTG groups.The postprandial malondialdehyde(MDA)response was lower after a 5:1 n-6/n-3 PUFA ratio meal than 1:1 and 20:1 ratio meals in HTG groups.The 1:1 ratio resulted in a lower postprandial reactive oxygen species(ROS)level than 5:1 and 10:1 n-6/n-3 PUFA ratios in NC groups.The results showed that a low n-6/n-3 PUFA ratio improved postprandial dysmetabolism induced by a high fat meal in NC and HTG rats.A high n-6/n-3 PUFA ratio increased the difference in postprandial metabolism between NC and HTG rats.

Effects of dietary ratio of n-6 to n-3 polyunsaturated fatty acids on immunoglobulins, cytokines, fatty acid composition, and performance of lactating sows and suckling piglets

This experiment was conducted to investigate the effects of dietary ratios of n-6：n-3 polyunsaturated fatty acids （PUFA） on the performance of lactating sows and their piglets. Thirty pregnant Landrace sows were assigned to one of three treatments from d 108 of gestation until weaning （26-29 d） and were fed diets containing different ratios of n-6：n-3 PUFA including 3：1,9：1 and 13：1. The effects on sow and litter production traits were examined together with an assessment of sow body condition. No differences were detected among the treatments for the daily feed intake of sows or changes in sow weight and back-fat levels during lactation （P 〉 0.05）. Litter size at d 14 and d 21 were tended to increase in 3：1 treatment compared with 9：1 and 13：1 treatments （P 〈 0.10）. Litter weight gain （1.77 kg/d） from d 0 to d 14 was tended to increase in 9：1 groups compared with the other two treatments （P 〈 0.10）. A significant difference was observed for the content of a -linolenic acid, total n-3 PUFA, and the ratio of n-6：n-3 PUFA in the colostrum, milk, and piglets plasma （P 〈 0.01）. The effects of different ratios of n-6：n-3 PUFA in sow diets on colostrum, milk and piglet plasma immunoglobulin concentrations are studied. No difference was observed among treatments in the concentrations of IgM, and IgA in colostrum （P 〉 0.05）. A great significant difference for IgG concentration was observed among 3 group in colostrum. A great significant difference for IgA, and IgM （P〈 0.01） concentrations in piglet plasma at d14 and a significant difference for IgG（P 〈 0.05） was observed at d14. Furthermore, at d 21 of lactation, piglet plasma IgG and IgA concentration were greater in 3：1 compared with 13：1 group （P 〈 0.01）. In summary, the current study demonstrated that altering the ratio of n-6：n-3 PUFA in lactating sow diet had an effect on the immune component including immunoglobulin and cytokines, and it tended to increase the litter average daily gain and improve the immune status of piglets when dietary ratio of n-6：n-3 PUFA was 9：1.

Effects of the Ratio of n-6∶n-3 Polyunsaturated Fatty Acids in the Maternal Diet on Immunoglobulins,Fatty Acid Composition,and Performance of Lactating Sows and Suckling Piglets under Conditions of Low Maternal Energy Intake

This experiment was conducted to investigate the effects of different ratios of n-6 ∶ n-3 polyunsaturated fatty acids （PUFA） in sow diets on the performance of lactating sows and their piglets at low digestible energy intakes. Twenty-one pregnant Landrace sows were assigned to one of three diets from day 108 of gestation until weaning （26 to 29 d） ,containing n-6∶n-3 PUFA ratios of 3 ∶1,8 ∶1 and 11 ∶1. The effects on sow and litter production traits and on sow body condition were examined. There were no differences among treatments in daily feed intakes or in changes in sow weight and back-fat levels during lactation. Litter size,litter weight at birth and weaning and litter average daily weight gain were also unaffected by treatment. As expected, large differences were observed in n-6 and n-3 fatty acids in the colostrum and plasma of sows and piglets （P 〈 0. 01） . The ratio of n6 ∶ n-3 PUFA the diet was positively correlated with those in colostrum,sow plasma and piglet plasma （R 2= 0. 55,0. 80 and 0. 80,respectively） . Sow plasma insulin and IGF-I levels at weaning were unaffected by the treatments. Plasma leptin （P 〈 0. 05） concentrations were increased in sows fed the diet with a n-6∶n-3 ratio of 8 ∶ 1. Immunoglobulin concentrations in colostrum were not altered by dietary treatment. Plasma IgG concentrations at d 14 were highest in piglets from sows fed the 8 ∶ 1 ratio of n-6∶n-3. Furthermore,this group had the highest IgA concentrations at day 21 of lactation compared with the other two groups （P 〈 0. 05） . In summary,our study demonstrated that at low digestible energy levels,altering the ratio of n-6 ∶ n-3 PUFA in the diets for lactating sows affected immune components and the fatty acid composition of lactating sows and their piglets. Further studies are needed to examine whether higher levels of fat supplementation than those used in the present study （1. 5%） can successfully enhance performance.

The Relationships between Erythrocyte Membrane n-6 to n-3 Polyunsaturated Fatty Acids Ratio and Blood Lipids and C-reactive Protein in Chinese Adults:An Observational Study

Objective To investigate the relationships between erythrocyte membrane n-6：n-3 PUFAs ratio and blood lipids and high sensitivity C-reactive protein （hs-CRP）. Methods The Chinese and a observational study consisted of a population-based cross-sectiona subsequent 1-year follow-up study of 171 subjects with the fasting cholesterol of 5.13-8.00 mmol/L. study of 456 plasma total Results In the cross-sectional analysis, plasma low-density lipoprotein cholesterol （LDL-c） had a significant and negative association with the erythrocyte membrane n-6：n-3 PUFAs ratio （P for trend=0.019） after adjusting for sex, age and total PUFA percentage. In the follow-up study, 171 subjects were categorized into quartiles by the changes of n-6：n-3 ratio in erythrocyte membrane （△=month 12-month 0）. In the top quartile whose ratios of n-6：n-3 increased by an average of 1.25 during the follow-up, the LDL-c-lowering extent was 3.3 times of that in the lowest quartile whose ratios of n-6：n-3 decreased by an average of 1.13 （-1.07 mmol/L v.s. -0.32 mmol/L）. The hsCRP decreased by 0.11 mg/dL in the lowest quartile while increasing by 0.10 mg/dL in the top quartile （P for difference=0.052）. Conclusion Our results suggested that the balance between n-6 and n-3 fatty acids may optimize the cardiovascular benefits from dietary PUFAs.

Beneficial n-3 polyunsaturated fatty acid levels and n6:n3 ratios after 4-week EPA + DHA supplementation associated with reduced CRP: A pilot study in healthy young adults

Chronic systemic inflammation is associated with many conditions of aging such as atherosclerosis. Lowering high n-6:n-3 polyunsaturated fatty acid (PUFA) ratios are commonly found in Western diets aids in preventing inflammatory-related diseases. However, it is not clear whether dietary interventions designed to alter n-6:n-3 PUFA ratios can reduce systemic inflammation in younger adults. Studies that evaluate PUFA intake often use subjective data from food frequency questionnaires or food records rather than more precise physiological measures of PUFAs (e.g. plasma levels). Therefore, the purpose of this pilot study that analyzed data from the experimental parent study of younger adults (n = 18), was to determine whether plasma PUFA levels were associated with levels of C-reactive protein (CRP), an inflammatory marker, and if supplementation with n-3 PUFAs was correlated with rising n-3 PUFA concentrations in plasma and decreasing n-6:n-3 ratios. In the parent study, participants received daily either placebo or n-3 PUFA softgels (1.6 g eicosapentaenoic acid [EPA] and 1.2 g docosahexaenoic acid [DHA]). EPA and DHA are the biologically active components in fish oil. Measures included blood for PUFA quantification at baseline and four weeks later, when blister wounds were created and wound fluid and saliva were collected. The saliva samples were used to measure CRP in the present study. We report that CRP was significantly and negatively correlated with total n-3 PUFAs (tau-β = ?0.373, p = 0.031) and positively correlated with n-6:n-3 ratios (tau-β = 0.320, p = 0.063). Those consuming EPA + DHA supplements had significantly higher concentrations of total n-3 PUFAs and significantly lower n-6:n-3 ratios (p The present study has shown that beneficial levels of n-3 PUFAs and n-6:n3 ratios were achieved with 4-weeks of EPA + DHA supplementation and were associated with reduced CRP in young adults. EPA + DHA supplementation for some young adults may help prevent inflammatory conditions later in life.

Influence of Replacement Time and Rate of Soybean Oil by Flax Oil in the Leghorn Hens＇ Diet on Polyunsaturated Fatty Acid Profile in Eggs

The study was aimed to evaluate the effect of replacement time and rate of soybean oil by flax oil （FO） on polyunsaturated fatty acid （PUFA） profile in egg of laying hens. Two hundred White Leghorn hens with 30 weeks of age were divided into five treatments with four replicates of l 0 birds each. Treatments were assigned randomly and consisted of 0.00%, 0.75%, 1.50%, 2.25% and 3.00% FO in commercial corn-soybean meal diets, in which the soybean oil was partially replaced. The experiment was conducted for 90 d. The main research findings were that feed intake, egg production rate, egg weight and feed conversion were not influenced by time and dietary treatment. Fatty acid content was significantly altered （P 〈 0.05） by FO, showing a progressive increase in egg n-3 fatty acid （especially docosahexaenoic acids （DHA） and eicosapentaenoic acids （EPA）） when FO was added. Levels of EPA and DHA were higher （P 〈 0.05） in the egg lipids of FO fed hens than those in the control group. However, no significant differences were observed either in egg weight or egg production among groups. The highest incorporation （P 〈 0.05） of total n-3 fatty acid content in eggs was obtained with 3% FO/kg. This increase was proportional to FO inclusion levels in the diets.

Azacarbazole n-3 and n-6 polyunsaturated fatty acids ethyl esters nanoemulsion with enhanced efficacy against Plasmodium falciparum

Alternative therapies are necessary for the treatment of malaria due to emerging drug resistance.However,many promising antimalarial compounds have poor water solubility and suffer from the lack of suitable delivery systems,which seriously limits their activity.To address this problem,we synthesized a series of azacarbazoles that were evaluated for antimalarial activity against D10(chloroquine-sensitive)and W2(chloroquine-resistant)strains of P.falciparum.The most active compound,9H-3-azacarbazole(3),was encapsulated in a novel o/w nanoemulsion consisting of ethyl esters of polyunsaturated fatty acids n-3 and n-6 obtained from flax oil as the oil phase,Smix(Tween 80 and Transcutol HP)and water.This formulation was further analyzed using transmission electron microscopy,dynamic light scattering and in vitro and in vivo studies.It was shown that droplets of the 3-loaded nanosystem were spherical,with satisfactory stability,without cytotoxicity towards fibroblasts and intestinal cell lines at concentrations corresponding to twice the IC50 for P.falciparum.Moreover,the nanoemulsion with this type of oil phase was internalized by Caco-2 cells.Additionally,pharmacokinetics demonstrated rapid absorption of compound 3(tmax=5.0 min)after intragastric administration of 3-encapsulated nanoemulsion at a dose of 0.02 mg/kg in mice,with penetration of compound 3 to deep compartments.The 3-encapsulated nanoemulsion was found to be 2.8 and 4.2 times more effective in inhibiting the D10 and W2 strains of the parasite,respectively,compared to non-encapsulated 3.Our findings support a role for novel o/w nanoemulsions as delivery vehicles for antimalarial drugs.

Supplemental Feeding of Laying Hens with Wood Vinegar to Decrease the Ratio of n-6 to n-3 Fatty Acids in Eggs

A balanced ratio of fatty acids n-6 to n-3 in chicken eggs is important for health and to help prevent and manage obesity and other diseases.Traditionally,fish oil or flax seed has been utilized as feed additives to decrease the ratio of n-6 to n-3(n-6:n-3)fatty acids in eggs.The hull of spina date seed(HSDS)is a common agricultural waste product in China,from which wood vinegar(HSDSWV)may be derived.This study evaluated HSDSWV as a sup-plement in hen feeds to improve the quality of eggs and decrease the ratio of fatty acids n-6:n-3.HSDSWV was obtained via carbonization,and refined.Six concentrations(nil to 0.5%)of HSDSWV were prepared and fed to 6 hen groups,respectively,for 50 d.The fatty acids of the hen’s egg yolks were analyzed by gas chromatography/electron ionization-mass spectrometry(GC/EI-MS)in the selected ion monitoring(SIM)mode.The 0.2%HSDSWV resulted in the best egg yolk quality,with a lower percentage of linoleic acid(C18:2n6)and higher percentages of cis-5,8,11,14,17-eicosapentaenoic acid(C20:5n3)and cis-4,7,10,13,16,19-docosahexaenoic acid(C22:6n3),and thus a lower n-6:n-3 ratio compared with the other HSDSWV concentrations.In addition,the eggs contained higher levels of yolk fat and egg yolk than the controls did.In conclusion,to modify the fatty acid composition of hens’eggs and obtain a balanced ratio of n-6:n-3,0.2%HSDSWV may be considered suitable as a dietary supplement in hens’feed.

不饱和脂肪酸与抑郁症的相关性研究进展

抑郁症是最常见的精神障碍,以持久而显著的心境低落为特征。在我国,抑郁症的发病率呈逐年上升趋势。本文介绍近年来国内外对于不饱和脂肪酸与抑郁症的相关性研究结果,并进行回顾与总结。大量研究表明,较低的血清多不饱和脂肪酸(主要是ω-3脂肪酸)水平与抑郁症状的严重程度高度相关,较高的血清多不饱和脂肪酸水平与较低的抑郁症发病率以及较低的抑郁症状评分相关。而血清单不饱和脂肪酸(ω-9脂肪酸)被证实与抑郁症状无相关性。较低的ω-6脂肪酸/ω-3脂肪酸比值可能预示较好的预后。血清多不饱和脂肪酸水平或可成为一种预测抑郁症发生率及抑郁症状严重度的参考依据。

锦鲤Δ6脂肪酸去饱和酶基因克隆及表达研究

旨在揭示变温鱼类合成HUFAs过程中的关键酶及其功能,以锦鲤(Cyprinus carpiokoi)为材料,通过RT-PCR扩增获得了一段长度为854 bp的Δ6脂肪酸去饱和酶基因的c DNA序列片段,并对其进行了序列分析和蛋白产物结构预测。结果显示,该片段与南亚野鲮(Labeo rohita)Δ6脂肪酸去饱和酶基因有93.5%的同源性,编码的蛋白产物具有典型的Δ6脂肪酸去饱和酶结构特点,包含2个组氨酸保守区(HDFGH、HFQHH)和2个跨膜结构域。荧光定量PCR表明,Δ6脂肪酸去饱和酶在锦鲤肝脏中的表达量最高,在肠、脑和心脏中表达量依次降低,而在肌肉和鳃的表达量相对较低。幼鱼在不同培养水温下,各组织中该基因的表达量均随温度的下降而升高,以适应低温环境。

Effect of n-3 HUFA levels in rotifers and Artemia on growth and survival of larval black sea bream (Sparus macrocephalus)

Requirement for dietary n-3 HUFA (n-3 highly unsaturated fatty acid) for growth and survival of black sea bream (Sparus macrocephalus) larvae was studied using rotifers and Artemia at various levels of n-3 HUFA. Five treatments with rotifers and Artemia differing in n-3 HUFA were prepared by enriching them with various oil emulsions. Results indicated that dietary n-3 HUFA significantly influence fish n-3 HUFA levels and are essential for growth and survival of black sea bream larvae. The results also indicated that the incorporation of n-3HUFA TG (triacylglycerols) into tissues of larval black sea bream was more effective from natural fish oil in comparison with n-3 HUFA fatty acid ethyl esters from ethyl-esterified oil.

不饱和脂肪酸与炎症性肠病因果关系的孟德尔随机化分析

背景:炎症性肠病(IBD)是一种慢性复发性胃肠道炎症性疾病,包括溃疡性结肠炎(UC)和克罗恩病(CD)。目前尚不清楚不饱和脂肪酸与IBD之间是否存在因果关系。目的:采用两样本孟德尔随机化分析探究不饱和脂肪酸与IBD之间的因果关系。方法:不饱和脂肪酸和IBD的全基因组关联研究(GWAS)数据均来源于网络公开数据库。采用逆方差加权分析法进行两样本孟德尔随机化分析,使用加权中位数法和MR⁃Egger回归分析验证因果效应,以OR及其95%CI评价不饱和脂肪酸与IBD风险的因果关系。结果:ω⁃6脂肪酸与CD无直接因果关系,与UC有直接因果关系,逆方差加权分析结果显示ω⁃6脂肪酸基因水平每增加一个标准差,UC风险增加16%(OR=1.16,95%CI:1.00~1.36,P=0.04)。而ω⁃3脂肪酸、单不饱和脂肪酸与IBD之间均未发现因果关系。结论:ω⁃6脂肪酸可能仅与UC存在因果关系,ω⁃3脂肪酸、单不饱和脂肪酸与IBD之间均未发现因果关系。

Dietary fatty acids and bioactive fatty acid metabolites in alcoholic liver disease

Alcoholic liver disease(ALD)comprises a spectrum of liver pathology,including steatosis,steatohepatitis,and cirrhosis.Previous work from our group and others suggests that dietary fat,both the amount and composition,plays a pivotal role in ALD development and progression;however,the impact of specific dietary fatty acids on ALD pathogenesis is not fully elucidated.Preclinical rodent models of ALD revealed the deleterious effects of omega-6 polyunsaturated fatty acids(n-6 PUFAs),specifically linoleic acid(LA),and this may be partially attributed to the increased levels of pro-inflammatory oxidized LA metabolites.There is limited understanding regarding the role of omega-3 polyunsaturated fatty acids(n-3 PUFAs,such as alpha-linolenic acid,eicosapentaenoic acid,and docosahexaenoic acid),and bioactive n-3 PUFAderived lipid molecules in ALD.Given that majority of n-6 and n-3 PUFAs-derived metabolites are potent endogenous signaling molecules,knowledge regarding the changes in these lipid mediators may shed new light on the mechanisms contributing to ALD pathogenesis and reveal novel therapeutic targets and biomarkers of this disease.The current review summarizes relevant scientific literature regarding the role of dietary fat,distinct fatty acids,and bioactive fatty acid metabolites in ALD,and highlights recent advances in the field.

亚麻籽油组分的功能活性研究进展

亚麻籽油是一种天然的保健植物功能性油脂,含有大量的不饱和脂肪酸,含量最大的分别是α-亚麻酸和亚油酸,这两种多不饱和脂肪酸在机体代谢、生长发育中发挥着不可或缺的作用,此外亚麻籽油中还含有多种生物活性物质,如;其他不饱和脂肪酸:二十二碳六烯酸(DHA)、二十碳五烯酸(EPA)和前列腺素(PG)等;亚麻籽环肽、维生素E、亚麻木酚素和油酸、黄酮类等,也在机体中起着积极作用。本文主要论述了亚麻籽油对于人体具有的多种功能,如:α-亚麻酸和维生素E自身对机体的抗氧化效果、α-亚麻酸在体液和基因水平上的降血脂作用、协调胰岛素的降血糖作用;还有亚麻木酚素和亚麻籽环肽在免疫协调中的抗炎抗癌作用;多不饱和脂肪酸在防治心血管疾病、抗衰老和抗氧化、调节肠道菌群、增强视力和抗菌等方面发挥作用等。

ω-3不饱和脂肪酸的生物学作用

由于人们的膳食中饱和脂肪酸 /多不饱和脂肪酸 (S/P)及 ω- 6不饱和脂肪酸 /ω- 3不饱和脂肪酸的比例失调 ,使得心血管的发病率显著增加。正因为 ω- 3不饱和脂肪酸的摄入量相对不足 ,所以人们更加重视ω- 3不饱和脂肪酸在脂肪酸促进胎儿和新生儿的大脑发育 ,抗肿瘤和炎性反应及降低冠心病、高血压、动脉粥样硬化、二型糖尿病等发病率的特殊作用 ,这些作用源于ω- 3的某些生物学作用。本文阐述 ω-

脑黄金鸡蛋的研究进展

DHA 和 EPA 是ω-3 多不饱和脂肪酸,它们俗称脑黄金。世界卫生组织推荐ω-6/ω-3 在人体的比例最好在5:1 左右。我国居民摄入ω-3 普遍不足,所以生产富含ω-3(脑黄金)鸡蛋对社会具有重要意义。本文主要就脑黄金鸡蛋的研究现状和研究中存在的问题作一综述。

小品种食用油的脂肪酸营养功能

分析论述了ω-3系列多不饱和脂肪酸(ω-3PUFA)、ω-6系列多不饱和脂肪酸(ω-6 PUFA)、单不饱和脂肪酸(MUPA)的特殊营养功效,阐述了不同类型的小品种食用油的脂肪酸营养特征。

不饱和脂肪酸水平与不同性别血脂异常冠心病患者的关系

目的找出冠心病患者血脂异常情况下特征性的不饱和脂肪酸谱的含量变化规律。方法收集患者的年龄、性别、BMI指数等信息;通过检测患者外周血的三酰甘油水平与样本中的不饱和脂肪酸谱,重点关注ω-3族不饱和脂肪酸、ω-6族不饱和脂肪酸和二者比值。结果ω-3族不饱和脂肪酸谱中的α-C18:3(ALA,α-亚麻酸)和ω-6族不饱和脂肪酸谱中的γ-C18:3在不同血脂水平的患者中有显著性差异(P<0.05),在女性患者群体中更加突出。结论在血脂异常的女性患者中,γ-C18:3水平、EPA水平与血脂正常女性有显著性差异,男性患者则无明显差异,提示血脂异常的冠心病患者群体中不饱和脂肪酸谱的变化可能受到性别的影响。

杜氏盐藻DsFAD2基因的鉴定及缺氮胁迫下表达分析

[目的]w-6脂肪酸去饱和酶2(FAD2)催化油酸(18∶1)去饱和形成亚油酸(18∶2),是多不饱和脂肪酸合成过程中的第一个关键酶。为了探究DsFAD2在盐藻油脂合成积累中的作用。[方法]本文以运城盐湖分离的富油杜氏盐藻(Dunaliella salina)株系(YC-011)为材料,利用高保真PCR技术克隆盐藻DsFAD2基因,并对DsFAD2编码蛋白进行了跨膜区、高级结构及氨基酸序列比对等一系列生物信息学分析。应用qRT-PCR检测DsFAD2基因在氮(N)胁迫培养条件下的表达模式。[结果]DsFAD2蛋白定位于内质网中,有5个跨膜区,具有典型膜结合蛋白的结构特征。此外,DsFAD2也具有所有FAD2蛋白家族所特有的3个组氨酸保守区和芳香族氨基酸序列。系统发育分析发现杜氏盐藻DsFAD2与莱茵衣藻CrFAD2、团藻VcFAD2亲缘关系最近。qRT-PCR表明,与正常培养的杜氏盐藻相比,缺N培养显著诱导DsFAD2上调表达,N胁迫8d时DsFAD2的表达量是未胁迫对照的2.8倍。[结论]DsFAD2参与杜氏盐藻N胁迫响应,促进多不饱和脂肪酸形成,增强藻细胞抗逆性。本研究为进一步解析N胁迫条件下藻细胞油脂富集及响应分子机制提供了科学参考。

Dietary n-6：n-3 ratio and Vitamin E improve motility characteristics in association with membrane properties of boar spermatozoa

This study was aimed to evaluate the effects of dietary n-6：n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 × 2 factorial design with two n-6：n-3 ratios （14.4 and 6.6） and two Vitamin E levels （200 and 400 mg kg-1）. During 16 weeks of treatment, fresh semen was collected at weeks O, 8, 12, and 16 for measurements of motility characteristics, contents of fatty acids, membrane properties （membrane fluidity and membrane integrity）, and lipid peroxidation of the spermatozoa. The semen was diluted in Beltsville Thawing Solution （BTS） extender and stored at 17℃, and the sperm motility was assessed at 12, 36, 72, and 120 h of storage. The 6.6 n-6.n-3 dietary ratio increased the contents of n-3 polyunsaturated fatty acids （PUFAs） and docosabexaenoic acid （DHA） and improved the membrane integrity and membrane fluidity of the spermatozoa, resulting in notably increased total motility, sperm progressive motility, and velocity parameters of fresh semen. Feeding diet with Vitamin E （400 mg kg-1） prevented sperm lipid peroxidation, and resulted in higher total motility and sperm progressive motility in fresh and liquid stored semen. In conclusion, the adjustment of n-6.n-3 ratio （6.6） and supply of Vitamin E （400 mg kg-1） successfully improved sperm motility characteristics and thus may be beneficial to the fertility of boars, which might be due to the modification of the physical and functional properties of spermatozoa membrane in response to dietary supplementation.