Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report...Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial cells.Both the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 phenotype.In addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and YM1.Taken together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.展开更多
Ara h 2是花生主要过敏原之一,为开发食物中Ara h 2过敏原成分的快速检测方法,减少因误食导致花生过敏事件的发生,该研究采用鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,通过棋盘法优化抗体工作浓度,建立了一种检测花生...Ara h 2是花生主要过敏原之一,为开发食物中Ara h 2过敏原成分的快速检测方法,减少因误食导致花生过敏事件的发生,该研究采用鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,通过棋盘法优化抗体工作浓度,建立了一种检测花生过敏原Ara h 2的间接双抗夹心化学发光酶免疫分析法,并对该方法的灵敏度、准确度、精密度和特异性进行评价。该方法的检出限为1.085 ng/mL,线性范围为3.12~200 ng/mL,添加回收率为78.30%~94.39%,批内和批间变异系数均小于10%,且特异性良好,与其他常见食物过敏原无交叉反应。该方法与相同抗体所建立的间接双抗夹心酶联免疫吸附测定(enzyme-linked immunosorbent assay, ELISA)方法相比,在灵敏度上表现出一定优势。该研究开发的化学发光酶免疫分析法可对花生食品生产过程中和消费前的Ara h 2过敏原成分检测提供可靠的技术支持。展开更多
针对氢燃料电池大功率电机驱动系统,提出一种以燃料电池为主动力源的轻量化级联H桥(cascadedH-bridge,CHB)型混合动力中压电机调速系统。所提系统由燃料电池/蓄电池/超级电容的混合动力源供电,基于四有源桥(quad activebridge,QAB)与CH...针对氢燃料电池大功率电机驱动系统,提出一种以燃料电池为主动力源的轻量化级联H桥(cascadedH-bridge,CHB)型混合动力中压电机调速系统。所提系统由燃料电池/蓄电池/超级电容的混合动力源供电,基于四有源桥(quad activebridge,QAB)与CHB子模块互联的两级变换器(cascaded H-bridges with quad active bridge,CHB-QAB)作为调速变换器。CHB-QAB通过四绕组高频变压器将各子模块进行内部互联,采用单边同步双边移相调制的策略,使得所有子模块呈现开关电容特性,在不依赖复杂控制的前提下,减小子模块电容的容值,提升系统的功率密度。针对三类动力源,采用基于低通滤波(lowpassfilter,LPF)的能量管理策略,保证电机实际运行过程中的有效功率分配,解决燃料电池对电机动态响应缓慢和燃料饥饿现象等问题。最后通过仿真与实验对所提轻量化电机调速系统进行验证。展开更多
基金supported by the National Natural Science Foundation of China,Nos.82171429,81771384a grant from Wuxi Municipal Health Commission,No.1286010241190480(all to YS)。
文摘Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial cells.Both the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 phenotype.In addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and YM1.Taken together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.
文摘Ara h 2是花生主要过敏原之一,为开发食物中Ara h 2过敏原成分的快速检测方法,减少因误食导致花生过敏事件的发生,该研究采用鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,通过棋盘法优化抗体工作浓度,建立了一种检测花生过敏原Ara h 2的间接双抗夹心化学发光酶免疫分析法,并对该方法的灵敏度、准确度、精密度和特异性进行评价。该方法的检出限为1.085 ng/mL,线性范围为3.12~200 ng/mL,添加回收率为78.30%~94.39%,批内和批间变异系数均小于10%,且特异性良好,与其他常见食物过敏原无交叉反应。该方法与相同抗体所建立的间接双抗夹心酶联免疫吸附测定(enzyme-linked immunosorbent assay, ELISA)方法相比,在灵敏度上表现出一定优势。该研究开发的化学发光酶免疫分析法可对花生食品生产过程中和消费前的Ara h 2过敏原成分检测提供可靠的技术支持。
文摘针对氢燃料电池大功率电机驱动系统,提出一种以燃料电池为主动力源的轻量化级联H桥(cascadedH-bridge,CHB)型混合动力中压电机调速系统。所提系统由燃料电池/蓄电池/超级电容的混合动力源供电,基于四有源桥(quad activebridge,QAB)与CHB子模块互联的两级变换器(cascaded H-bridges with quad active bridge,CHB-QAB)作为调速变换器。CHB-QAB通过四绕组高频变压器将各子模块进行内部互联,采用单边同步双边移相调制的策略,使得所有子模块呈现开关电容特性,在不依赖复杂控制的前提下,减小子模块电容的容值,提升系统的功率密度。针对三类动力源,采用基于低通滤波(lowpassfilter,LPF)的能量管理策略,保证电机实际运行过程中的有效功率分配,解决燃料电池对电机动态响应缓慢和燃料饥饿现象等问题。最后通过仿真与实验对所提轻量化电机调速系统进行验证。
