We have fabricated an unexpected type of supported planar bilayer composed of receptor phospholipids and single-chained diacetylenes as fluorogenic reporters using protruded anchor moieties with a positive terminal ch...We have fabricated an unexpected type of supported planar bilayer composed of receptor phospholipids and single-chained diacetylenes as fluorogenic reporters using protruded anchor moieties with a positive terminal charge.Nanoscale topographical and surface thermodynamic analyses,as well as molecular dynamics simulations,revealed the coexistence of well-dispersed liquid-condensed(L_(c))domains forming nano-islands and liquid-expanded(L_(e))region in the planar bilayer,enhancing sensitivity against a prototype of ubiquitous membrane-associated antimicrobial peptides,melittin.The L_(e)regions,acting as target receptors,enabled sensitive detection as the melittin adsorbed and inserted into these regions due to strong hydrophobic interactions between phospholipids and melittin.The L_(c)domains,serving as signal reporters,enabled diacetylenes to assemble,polymerize,and fluoresce in response to the insertion of melittin into the L_(e)regions.Thus,biphasic nanodomains of the planar lipid bilayer finally endowed this sensor system with a detection range of 100μMto 50 nM and a limit of detection(LOD)of∼37 nM for melittin.This exceeded the operational performance of the colorimetric polydiacetylene vesicle solution 45 times,which reportedly ranged from 100 to 4μM with an LOD of∼1.7μM.展开更多
Herein,a novel strategy for regulating the phase structure was used to significantly enhance the recoverable energy storage density(Wrec)and the thermal stability via designing the(1-x)[(Bi_(0.5)Na_(0.5))_(0.7)Sr_(0.3...Herein,a novel strategy for regulating the phase structure was used to significantly enhance the recoverable energy storage density(Wrec)and the thermal stability via designing the(1-x)[(Bi_(0.5)Na_(0.5))_(0.7)Sr_(0.3)TiO_(3)]-xBiScO_(3)((1-x)BNST-xBS)relaxor ferroelectric ceramics.The incorporation of BS into BNST ceramics markedly increases the local micro-structure disorder,causing a high polarization and inhibiting polarization hysteresis for 0.95BNST-0.05BS ceramics,leading to a large Wrec of 5.41 J/cm^(3)with an ideal efficiency(h)of 78.5%.Meanwhile,transmission electron microscope(TEM)results further proved that the nano-domain structure and the tetragonal(P4bm)phase superlattice structure of 0.95BNST-0.05BS ceramics possess an excellent thermal stability(20-200℃).An outstanding Wrec value of 3.18×(1.00±0.03)J/cm^(3)and an h value of 74.500±0.025 are achieved under a temperature range from 20℃to 200℃.This work provides a promising method for phase-structure design that can make it possible to apply temperature-insensitive ceramic dielectrics with a high energy storage density in harsh environments.展开更多
基金This work was supported by the National Research Foundation of Korea(grant nos.NRF-2021R1A2C3009955 and 2017M3D1A1039421)and a Korea University Grant.
文摘We have fabricated an unexpected type of supported planar bilayer composed of receptor phospholipids and single-chained diacetylenes as fluorogenic reporters using protruded anchor moieties with a positive terminal charge.Nanoscale topographical and surface thermodynamic analyses,as well as molecular dynamics simulations,revealed the coexistence of well-dispersed liquid-condensed(L_(c))domains forming nano-islands and liquid-expanded(L_(e))region in the planar bilayer,enhancing sensitivity against a prototype of ubiquitous membrane-associated antimicrobial peptides,melittin.The L_(e)regions,acting as target receptors,enabled sensitive detection as the melittin adsorbed and inserted into these regions due to strong hydrophobic interactions between phospholipids and melittin.The L_(c)domains,serving as signal reporters,enabled diacetylenes to assemble,polymerize,and fluoresce in response to the insertion of melittin into the L_(e)regions.Thus,biphasic nanodomains of the planar lipid bilayer finally endowed this sensor system with a detection range of 100μMto 50 nM and a limit of detection(LOD)of∼37 nM for melittin.This exceeded the operational performance of the colorimetric polydiacetylene vesicle solution 45 times,which reportedly ranged from 100 to 4μM with an LOD of∼1.7μM.
基金supported by the National Natural Science Foundation of China(Grant No.51902167,51971005)Natural Science Foundation of Zhejiang Province(Grant No.LY21E020002)Natural Science Foundation of Ningbo City(Grant No.2021J064,2021J097).
文摘Herein,a novel strategy for regulating the phase structure was used to significantly enhance the recoverable energy storage density(Wrec)and the thermal stability via designing the(1-x)[(Bi_(0.5)Na_(0.5))_(0.7)Sr_(0.3)TiO_(3)]-xBiScO_(3)((1-x)BNST-xBS)relaxor ferroelectric ceramics.The incorporation of BS into BNST ceramics markedly increases the local micro-structure disorder,causing a high polarization and inhibiting polarization hysteresis for 0.95BNST-0.05BS ceramics,leading to a large Wrec of 5.41 J/cm^(3)with an ideal efficiency(h)of 78.5%.Meanwhile,transmission electron microscope(TEM)results further proved that the nano-domain structure and the tetragonal(P4bm)phase superlattice structure of 0.95BNST-0.05BS ceramics possess an excellent thermal stability(20-200℃).An outstanding Wrec value of 3.18×(1.00±0.03)J/cm^(3)and an h value of 74.500±0.025 are achieved under a temperature range from 20℃to 200℃.This work provides a promising method for phase-structure design that can make it possible to apply temperature-insensitive ceramic dielectrics with a high energy storage density in harsh environments.
