A silk fibroin(SF)spongy wound dressing incorporated with silver nanoparticles(Ag-NPs)was developed for biomedical applications.Ag-NPs were efficiently synthesized in situ via ultra violet(UV)with AgNO_(3) as precurso...A silk fibroin(SF)spongy wound dressing incorporated with silver nanoparticles(Ag-NPs)was developed for biomedical applications.Ag-NPs were efficiently synthesized in situ via ultra violet(UV)with AgNO_(3) as precursor and silk fibroin as reducing and protecting agent,respectively.After lyophilization,the formed silk fibroin spongy wound dressing(SFWD)exhibited polyporous morphology and inner lamellae structures,with uniform dispersion of Ag-NPs.The porous structure provided SFWD with the ability to absorb tissue exudatealmost 6 times of its own weight,which could guarantee the sustained release of Ag-NPs.By methanol treatment,SFWD showed much improved mechanical properties and more stable to protease XIV.The cyto-compatibility of SFWD was supported by normal adherence and proliferation of NIH3T3 fibroblasts in sponges extracting culture medium.More important,the SFWD showed significant growth inhibition in both plate culture assays and bacterial suspension assays,with Gram-positive(Staphylococcus aureus)and Gram-negative(Pseudomonas aeruginosa and Escherichia coli).In a cutaneous excisional mouse model,the average healing rates of SFWD was significantly higher than control and commercial bandages.The hematoxylin-eosin(HE)staining results of the wound section also showed that SFWD could recruit more cells and promote tissue formation on the wound edges.展开更多
Electrospinning technique was used for the fabrication of poly( vinyl alcohol)( PVA) / regenerated silk fibroin( SF)composite nanofibers,loaded with ciprofloxacin HCl( CipH Cl) as a wound dressing.Electrospun PVA / SF...Electrospinning technique was used for the fabrication of poly( vinyl alcohol)( PVA) / regenerated silk fibroin( SF)composite nanofibers,loaded with ciprofloxacin HCl( CipH Cl) as a wound dressing.Electrospun PVA / SF / CipH Cl composite nanofibers were stabilized against dissolving in water by heating in an oven at155 ℃ for 5 min.Incorporation of CipH Cl into electrospun nanofibers was confirmed by SEM and FT-IR spectra.Further the mechanical properties test illustrated that the addition of CipH Cl enhanced the mechanical properties of PVA and PVA / SF nanofibers.The antibacterial activities against Escherichia coli( E.coli)( gram-negative) and Staphylococcus aureus( S.aureus)( gram-positive) organisms were evaluated by disk diffusion method;and results suggested that electrospun PVA / CipH Cl and PVA / SF /CipH Cl composite nanofibers showed a remarkable antibacterial activity.展开更多
Although the treatment of burn wounds has made great progress, the incidence of wound infection is still the main cause of high mortality. In this study, a silk fibroin (SF) scaffold wound dressing incorporated with G...Although the treatment of burn wounds has made great progress, the incidence of wound infection is still the main cause of high mortality. In this study, a silk fibroin (SF) scaffold wound dressing incorporated with Gentamicin Sulfate (GS) was developed for the treatment of burn infected wounds, in which GS was used as anti-bacterial agent. GS was mixed with silk fibroin solution and then processed into GS-SF composite scaffold via electro-spinning. The results showed the scaffold exhibited uniform polyporous morphology with 80% porosity. Induced by methanol, the scaffold presented much improved mechanical properties and stability to protease <em>XIV</em>. More important, the scaffold presented significant growth inhibition on both Gram-positive (<em>Staphylococcus aureus</em>) and Gram-negative (<em>Pseudomonas aeruginosa and Escherichia coli</em>) bacteria.展开更多
Natural biopolymers have excellent biocompatibility and biodegradability;they can be used as biomedical materials for wound healing.In this work,the Silk Fibroin(SF)andκ-carrageenan(κ-Car)composite films containing ...Natural biopolymers have excellent biocompatibility and biodegradability;they can be used as biomedical materials for wound healing.In this work,the Silk Fibroin(SF)andκ-carrageenan(κ-Car)composite films containing Zinc-doped Bioactive Glass(ZBG)have been fabricated for wound closure applications.The as-fabricated SF-κ-Car/ZBG composite films have excellent stretchability and foldability,which facilitates them for application as wound dressing.They also exhibit excellent hydrophilicity and water-absorption capacity,which can effectively absorb wound exudate and keep the wound sites under moist state.In addition,the composite films have a good antibacterial effect against S.aureus and E.coli in vitro,which can reduce the risk of wound infection.Their excellent cell compatibility is confirmed by the CCK-8 assay.The strong vascular proliferation and wound regeneration are found in SF-κ-Car/ZBG composite films on a mouse skin wound model.The SF-κCar/ZBG composite films can inhibit the secretion of inflammatory factors,and stimulate the production of vascular factors and collagen fibers.The results derived from the performed investigations revealed that the SF-κ-Car/ZBG composite films are a promising candidate dressing for wound healing applications.展开更多
基金This research is supported by Chongqing Research Program of Basic Research and Frontier Technology(No.cstc2019jcyj-msxmX0164 to Jiang)Science and Technology Research Program of Chongqing Municipal Education Commission(No.KJQN201900414 to Jiang)Special Project on Philosophy and Social Sciences of Chongqing Medical University(No.201704 to Xiong).
文摘A silk fibroin(SF)spongy wound dressing incorporated with silver nanoparticles(Ag-NPs)was developed for biomedical applications.Ag-NPs were efficiently synthesized in situ via ultra violet(UV)with AgNO_(3) as precursor and silk fibroin as reducing and protecting agent,respectively.After lyophilization,the formed silk fibroin spongy wound dressing(SFWD)exhibited polyporous morphology and inner lamellae structures,with uniform dispersion of Ag-NPs.The porous structure provided SFWD with the ability to absorb tissue exudatealmost 6 times of its own weight,which could guarantee the sustained release of Ag-NPs.By methanol treatment,SFWD showed much improved mechanical properties and more stable to protease XIV.The cyto-compatibility of SFWD was supported by normal adherence and proliferation of NIH3T3 fibroblasts in sponges extracting culture medium.More important,the SFWD showed significant growth inhibition in both plate culture assays and bacterial suspension assays,with Gram-positive(Staphylococcus aureus)and Gram-negative(Pseudomonas aeruginosa and Escherichia coli).In a cutaneous excisional mouse model,the average healing rates of SFWD was significantly higher than control and commercial bandages.The hematoxylin-eosin(HE)staining results of the wound section also showed that SFWD could recruit more cells and promote tissue formation on the wound edges.
基金"111 Project" Biomedical Textile Materials Science and Technology,China(No.B07024)National Natural Science Foundations of China(Nos.31070871,31271035)+1 种基金Science and Technology Commission of Shanghai Municipality,China(No.11nm0506200)Ph.D.Programs Foundation of Ministry of Education of China(No.20130075110005)
文摘Electrospinning technique was used for the fabrication of poly( vinyl alcohol)( PVA) / regenerated silk fibroin( SF)composite nanofibers,loaded with ciprofloxacin HCl( CipH Cl) as a wound dressing.Electrospun PVA / SF / CipH Cl composite nanofibers were stabilized against dissolving in water by heating in an oven at155 ℃ for 5 min.Incorporation of CipH Cl into electrospun nanofibers was confirmed by SEM and FT-IR spectra.Further the mechanical properties test illustrated that the addition of CipH Cl enhanced the mechanical properties of PVA and PVA / SF nanofibers.The antibacterial activities against Escherichia coli( E.coli)( gram-negative) and Staphylococcus aureus( S.aureus)( gram-positive) organisms were evaluated by disk diffusion method;and results suggested that electrospun PVA / CipH Cl and PVA / SF /CipH Cl composite nanofibers showed a remarkable antibacterial activity.
文摘Although the treatment of burn wounds has made great progress, the incidence of wound infection is still the main cause of high mortality. In this study, a silk fibroin (SF) scaffold wound dressing incorporated with Gentamicin Sulfate (GS) was developed for the treatment of burn infected wounds, in which GS was used as anti-bacterial agent. GS was mixed with silk fibroin solution and then processed into GS-SF composite scaffold via electro-spinning. The results showed the scaffold exhibited uniform polyporous morphology with 80% porosity. Induced by methanol, the scaffold presented much improved mechanical properties and stability to protease <em>XIV</em>. More important, the scaffold presented significant growth inhibition on both Gram-positive (<em>Staphylococcus aureus</em>) and Gram-negative (<em>Pseudomonas aeruginosa and Escherichia coli</em>) bacteria.
基金supported by the Zhejiang Provincial Natural Science Foundation(LBY20H110001,LY18E030006)National Natural Science Foundation of China(51873194,51373155).
文摘Natural biopolymers have excellent biocompatibility and biodegradability;they can be used as biomedical materials for wound healing.In this work,the Silk Fibroin(SF)andκ-carrageenan(κ-Car)composite films containing Zinc-doped Bioactive Glass(ZBG)have been fabricated for wound closure applications.The as-fabricated SF-κ-Car/ZBG composite films have excellent stretchability and foldability,which facilitates them for application as wound dressing.They also exhibit excellent hydrophilicity and water-absorption capacity,which can effectively absorb wound exudate and keep the wound sites under moist state.In addition,the composite films have a good antibacterial effect against S.aureus and E.coli in vitro,which can reduce the risk of wound infection.Their excellent cell compatibility is confirmed by the CCK-8 assay.The strong vascular proliferation and wound regeneration are found in SF-κ-Car/ZBG composite films on a mouse skin wound model.The SF-κCar/ZBG composite films can inhibit the secretion of inflammatory factors,and stimulate the production of vascular factors and collagen fibers.The results derived from the performed investigations revealed that the SF-κ-Car/ZBG composite films are a promising candidate dressing for wound healing applications.
