As a broad-spectrum antiviral nanoparticle,the cell membrane nanodecoy is a promising strategy for preventing viral infections.However,most of the cell membrane nanodecoys can only catch virus and cannot induce inacti...As a broad-spectrum antiviral nanoparticle,the cell membrane nanodecoy is a promising strategy for preventing viral infections.However,most of the cell membrane nanodecoys can only catch virus and cannot induce inactivation,which may bring about a considerably high risk of re-infection owing to the possible viral escape from the nanodecoys.To tackle this challenge,sulfated liposomes are employed to mimic the cell membrane glycocalyx for constructing an artificial cell membrane glycocalyx nanodecoy that exhibits excellent anti-coronavirus activity against HCoV-OC43,wild-type SARS-CoV-2,Alpha and Delta variant SARS-CoV-2 pseudovirus.In addition,this nanodecoy,loaded with surface sulfate groups as SARS-CoV-2 receptor arrays,can enhance the antiviral capability to virus inactivation through destroying the virus membrane structure and transfer the spike protein to postfusion conformation.Integrating bio-inspired recognition and inactivation of viruses in a single supramolecular entity,the artificial cell membrane nanodecoy opens a new avenue for the development of theranostic antiviral nanosystems,whose mass production is favored due to the facile engineering of sulfated liposomes.展开更多
Platelets buoy up cancer metastasis via arresting cancer cells, enhancing their adhesion, and facilitating their extravasation through the vasculature. When deprived of intracellular and granular contents, platelet de...Platelets buoy up cancer metastasis via arresting cancer cells, enhancing their adhesion, and facilitating their extravasation through the vasculature. When deprived of intracellular and granular contents, platelet decoys could prevent metastatic tumor formation. Inspired by these, we developed nanoplatesomes by fusing platelet membranes with lipid membranes(P-Lipo) to restrain metastatic tumor formation more efficiently. It was shown nanoplateletsomes bound with circulating tumor cells(CTC)efficiently, interfered with CTC arrest by vessel endothelial cells, CTC extravasation through endothelial layers, and epithelial-mesenchymal transition of tumor cells as nanodecoys. More importantly, in the mouse breast tumor metastasis model, nanoplateletsomes could decrease CTC survival in the blood and counteract metastatic tumor growth efficiently by inhibiting the inflammation and suppressing CTC escape. Therefore, nanoplatelesomes might usher in a new avenue to suppress lung metastasis.展开更多
基金supported by the National Key Research&Development Program of China(No.2021YFC2301204)Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering(2022SZ-TD010)the Fundamental Research Funds for the Central Universities(No.2022ZFJH003).We thank Lingyun Wu in the Center of Cryo-Electron Microscopy(CCEM),Zhejiang University for her technical assistance on Cryo-EM.
文摘As a broad-spectrum antiviral nanoparticle,the cell membrane nanodecoy is a promising strategy for preventing viral infections.However,most of the cell membrane nanodecoys can only catch virus and cannot induce inactivation,which may bring about a considerably high risk of re-infection owing to the possible viral escape from the nanodecoys.To tackle this challenge,sulfated liposomes are employed to mimic the cell membrane glycocalyx for constructing an artificial cell membrane glycocalyx nanodecoy that exhibits excellent anti-coronavirus activity against HCoV-OC43,wild-type SARS-CoV-2,Alpha and Delta variant SARS-CoV-2 pseudovirus.In addition,this nanodecoy,loaded with surface sulfate groups as SARS-CoV-2 receptor arrays,can enhance the antiviral capability to virus inactivation through destroying the virus membrane structure and transfer the spike protein to postfusion conformation.Integrating bio-inspired recognition and inactivation of viruses in a single supramolecular entity,the artificial cell membrane nanodecoy opens a new avenue for the development of theranostic antiviral nanosystems,whose mass production is favored due to the facile engineering of sulfated liposomes.
基金supported by the National Natural Science Foundation of China (81773283)。
文摘Platelets buoy up cancer metastasis via arresting cancer cells, enhancing their adhesion, and facilitating their extravasation through the vasculature. When deprived of intracellular and granular contents, platelet decoys could prevent metastatic tumor formation. Inspired by these, we developed nanoplatesomes by fusing platelet membranes with lipid membranes(P-Lipo) to restrain metastatic tumor formation more efficiently. It was shown nanoplateletsomes bound with circulating tumor cells(CTC)efficiently, interfered with CTC arrest by vessel endothelial cells, CTC extravasation through endothelial layers, and epithelial-mesenchymal transition of tumor cells as nanodecoys. More importantly, in the mouse breast tumor metastasis model, nanoplateletsomes could decrease CTC survival in the blood and counteract metastatic tumor growth efficiently by inhibiting the inflammation and suppressing CTC escape. Therefore, nanoplatelesomes might usher in a new avenue to suppress lung metastasis.
