In the present study,nine new cembrane-type diterpenoids,namely ximaonanolobatins A—I(1—9),together with six related known analogs(10—15)and four known tetraprenylated alkaloids malonganenones D,E,H,and K(16—19)we...In the present study,nine new cembrane-type diterpenoids,namely ximaonanolobatins A—I(1—9),together with six related known analogs(10—15)and four known tetraprenylated alkaloids malonganenones D,E,H,and K(16—19)were isolated from the soft coral Sinularia nanolobata collected off the Ximao Island in the South China Sea.The structures of 1—9 were robustly established by a combination of detailed spectroscopic analyses,chemical reactions,quantum mechanical(QM)-NMR methods,biogenetic consideration,and the comparison with those literature data.The absolute configuration of 1 was confirmed by X-ray diffraction analysis,and the absolute configuration of 2 was determined by QM-NMR calculations and chemical transformation.In addition,the absolute configuration of 3 was determined using the modified Mosher's method.All these isolates were evaluated for their anti-inflammatory and anti-tumor activities.The results showed that only compound 15 exhibited potential anti-inflammatory effect against LPS-induced TNF-αrelease in RAW264.7 macrophages with an IC50 value of 12.6μmol/L.展开更多
基金financially supported by the National Key Research and Development Program of China(No.2018YFC0310903)the Natural Science Foundation of China(No.81991521)the SKLDR/SIMM Project(SIMM2103ZZ-06).
文摘In the present study,nine new cembrane-type diterpenoids,namely ximaonanolobatins A—I(1—9),together with six related known analogs(10—15)and four known tetraprenylated alkaloids malonganenones D,E,H,and K(16—19)were isolated from the soft coral Sinularia nanolobata collected off the Ximao Island in the South China Sea.The structures of 1—9 were robustly established by a combination of detailed spectroscopic analyses,chemical reactions,quantum mechanical(QM)-NMR methods,biogenetic consideration,and the comparison with those literature data.The absolute configuration of 1 was confirmed by X-ray diffraction analysis,and the absolute configuration of 2 was determined by QM-NMR calculations and chemical transformation.In addition,the absolute configuration of 3 was determined using the modified Mosher's method.All these isolates were evaluated for their anti-inflammatory and anti-tumor activities.The results showed that only compound 15 exhibited potential anti-inflammatory effect against LPS-induced TNF-αrelease in RAW264.7 macrophages with an IC50 value of 12.6μmol/L.
