Deposition of β-amyloid protein(Aβ) is the main hallmark of Alzheimer's disease(AD), and it has been well recognized that Cu^(2+)-mediated Aβ aggregation plays a crucial role in AD pathological processes.Cu^(2+...Deposition of β-amyloid protein(Aβ) is the main hallmark of Alzheimer's disease(AD), and it has been well recognized that Cu^(2+)-mediated Aβ aggregation plays a crucial role in AD pathological processes.Cu^(2+)binding to Aβ can promote the production of reactive oxygen species(ROS) through Fenton-like reactions and produce more toxic Aβ-Cu^(2+)species under Cu^(2+)stimulation. Thus, the development of nanomaterials that can inhibit Cu^(2+)-mediated Aβ aggregation and degrade Aβ-Cu^(2+)complexes is considered an effective strategy for the prevention and treatment of AD. In this study, polydopamine nanoparticles(PDA NPs) were prepared and the results reveal that PDA NPs potently inhibit Cu^(2+)-mediated Aβaggregation and effectively reduce the formation of Aβ-Cu^(2+)complexes. In vitro experiments show that PDA NPs efficiently eliminate ROS generation catalyzed by Cu^(2+)or Aβ-Cu^(2+)complexes, thus rescuing cultured cells by reducing intracellular ROS levels. More importantly, PDA NPs can depolymerize Aβ-Cu^(2+)complexes, and the degradation of Aβ-Cu^(2+)complexes is promoted by near-infrared light irradiation due to their high photothermal conversion ability. In vivo studies reveal that PDA NPs significantly reduce the deposition of Aβ plaques in the presence of Cu^(2+)and extend the lifespan of AD nematodes from 11 to 14 d. Thus, the PDA NPs developed herein are multifunctional against Cu^(2+)-mediated Aβ aggregation for the potential prevention and treatment of AD.展开更多
This work aims to analyze the flow of electrically conducting MWCNTs-nanofluid over a stretching cylinder with the aggregation and non-aggregation effects of nanoparticles. The working fluid comprised a combination of...This work aims to analyze the flow of electrically conducting MWCNTs-nanofluid over a stretching cylinder with the aggregation and non-aggregation effects of nanoparticles. The working fluid comprised a combination of water and ethylene glycol, with volumetric proportions of (50:50) considered. Convective boundary constraints and modified Fourier law are implemented in heat transmission assessment. The mathematical flow model is formulated in the form of PDEs and is transformed into ODEs via similarity transformation. Numerical outcomes will be obtained with the use of the bvp4c technique and will be displayed with the help of graphs and tables. The results show that the surface drag coefficient is enhanced in the case of aggregation of nanoparticles whereas heat transfer rate is enhanced in the non-aggregation effect of nanoparticles. Furthermore, the temperature distribution enhances the increasing values of particle volume fraction in the case of aggregation effects of nanoparticles whereas temperature distribution lowers in the case of non-aggregation effect of nanoparticles. .展开更多
Citrate-reduced silver nanoparticles (Ag-NPs) are used extensively for surface-enhanced Raman scattering (SERS) studies, but are typically found to aggregate using an aggregation agent. This study is aimed at developi...Citrate-reduced silver nanoparticles (Ag-NPs) are used extensively for surface-enhanced Raman scattering (SERS) studies, but are typically found to aggregate using an aggregation agent. This study is aimed at developing a simple, stable, and reproducible aggregated method for Ag-NPs without any aggregation agents in aqueous solutions. The aggregation is induced by the process of centrifugation, water washing and ultrasonication. A mechanism based on the nonuniform distribution of capping ligands is proposed to account for the aggregated structure formation. UV-Vis-NIR extinction spectra and TEM allowed us to identify the existence of Ag-NPs aggregation. Further, due to the polydisperse mixture of Ag-NPs (20~65 nm) used in the present work, Ag-NPs are aggregated closely, which contribute to the observation of low-concentration SERS from the residual citrate layer or even the single-molecule SERS of R6Gon aggregation. After the evaporation of droplet of Ag-NPs aggregation on the Si substrate, citrate or R6Gcould also be detected but with marked redor blue-shifts.展开更多
Photodynamic therapy(PDT) employs accumulation of photosensitizers(PSs) in malignant tumor tissue followed by the light-induced generation of cytotoxic reactive oxygen species to kill the tumor cells. The success of P...Photodynamic therapy(PDT) employs accumulation of photosensitizers(PSs) in malignant tumor tissue followed by the light-induced generation of cytotoxic reactive oxygen species to kill the tumor cells. The success of PDT depends on optimal PS dosage that is matched with the ideal power of light. This in turn depends on PS accumulation in target tissue and light administration time and period.As theranostic nanomedicine is driven by multifunctional therapeutics that aim to achieve targeted tissue delivery and image-guided therapy, fluorescent PS nanoparticle(NP)accumulation in target tissues can be ascertained through fluorescence imaging to optimize the light dose and administration parameters. In this regard, zebrafish larvae provide a unique transparent in vivo platform to monitor fluorescent PS bio-distribution and their therapeutic efficiency. Using fluorescent PS NPs with unique aggregation-induced emission characteristics, we demonstrate for the first time the real-time visualization of polymeric NP accumulation in tumor tissue and, more importantly, the best time to conduct PDT using transgenic zebrafish larvae with inducible liver hyperplasia as an example.展开更多
A sensitivity analysis is performed to analyze the effects of the nanoparticle(NP)aggregation and thermal radiation on heat transport of the nanoliquids(titania based on ethylene glycol)over a vertical cylinder.The op...A sensitivity analysis is performed to analyze the effects of the nanoparticle(NP)aggregation and thermal radiation on heat transport of the nanoliquids(titania based on ethylene glycol)over a vertical cylinder.The optimization of heat transfer rate and friction factor is performed for NP volume fraction(1%≤φ≤3%),radiation parameter(1≤R_(t)≤3),and mixed convection parameter(1.5≤λ≤2.5)via the facecentered central composite design(CCD)and the response surface methodology(RSM).The modified Krieger and Dougherty model(MKDM)for dynamic viscosity and the Bruggeman model(BM)for thermal conductivity are utilized to simulate nanoliquids with the NP aggregation aspect.The complicated nonlinear problem is treated numerically.It is found that the temperature of nanoliquid is enhanced due to the aggregation of NPs.The friction factor is more sensitive to the volume fraction of NPs than the thermal radiation and the mixed convection parameter.Furthermore,the heat transport rate is more sensitive to the effect of radiative heat compared with the NP volume fraction and mixed convection parameter.展开更多
1 Introduction Nanoparticles are widely found in the ductile shear zone and it is considered to have a close relation with faulting.The sizes of these nanoparticles are generallyless than 100 nm.They have a variety of...1 Introduction Nanoparticles are widely found in the ductile shear zone and it is considered to have a close relation with faulting.The sizes of these nanoparticles are generallyless than 100 nm.They have a variety of morphologies like globular structure rod-like and tubular,by the order aggregating of these nanoparticles various aggregations展开更多
We present the results of molecular dynamics simulations of net positively charged fullerene nanoparticles in salt- free and salt-added solution. The aggregation of fullerene (C60)-like nanoparticle and counterion a...We present the results of molecular dynamics simulations of net positively charged fullerene nanoparticles in salt- free and salt-added solution. The aggregation of fullerene (C60)-like nanoparticle and counterion are studied in detail as a function of temperatures and a finite salt concentration. Our simulations show that the strong conformation changes as temperature changes. The net positively-charged nanoparticles do not repel each other but are condensed under proper temperatures. If salts are added, the aggregated nanoparticles will be disaggregated due to the Debye screening effect.展开更多
The current exploration focuses on the ethylene glycol(EG)based nanoliquid flow in a microchannel.The effectiveness of the internal heat source and linear radiation is reflected in the present investigation.The estima...The current exploration focuses on the ethylene glycol(EG)based nanoliquid flow in a microchannel.The effectiveness of the internal heat source and linear radiation is reflected in the present investigation.The estimation of suitable thermal conductivity model has affirmative impact on the convective heat transfer phenomenon.The examination is conceded with the nanoparticle aggregation demonstrated by the Maxwell-Bruggeman and Krieger-Dougherty models which tackle the formation of nanolayer.These models effectively describe the thermal conductivity and viscosity correspondingly.The dimensionless mathematical expressions are solved numerically by the Runge Kutta Fehlberg approach.A higher thermal field is attained for the Bruggeman model due to the formation of thermal bridge.A second law analysis is carried out to predict the sources of irreversibility associated with the thermal system.It is remarked that lesser entropy generation is obtained for the aggregation model.The entropy generation rate declines with the slip flow and the thermal heat flux.A notable enhancement in the Bejan number is attained by increasing the Biot number.It is established that the nanoparticle aggragation model exhibits a higher Bejan number in comparision with the usual flow model.展开更多
Melanin, the main photoreceptor in living organics, is largely abundant in pigmented hair tissues. Its promising properties have been widely studied in order to fully explore the corresponding applications. However, t...Melanin, the main photoreceptor in living organics, is largely abundant in pigmented hair tissues. Its promising properties have been widely studied in order to fully explore the corresponding applications. However, the photodegradating and aggregating natures of melanin, to some extent, restrict the development of this eco-friendly biomaterial. This study is conducted to overcome both disadvantages by a mature method that coating the original melanin nanoparticles with silica as shells. The results revealed that the aggregation of the melanin/silica nanoparticles (MSNP) was decreased obviously by a 5 h coating and appeared a uniformly mono-dispersed solution. The MSNP that coated for 20 h provided an efficient protection on the photodegradation of the melanin with a 50% maintenance of the melanin content compared with 16% of original melanin and 2% of MSNP-5 h.展开更多
In aqueous environment,organic matters may exert impacts on the aggregation behaviors of titanium dioxide nanoparticles( TiO2-NPs). Owing to the deficiency of studies on the aggregation of TiO2-NPs in the presence of ...In aqueous environment,organic matters may exert impacts on the aggregation behaviors of titanium dioxide nanoparticles( TiO2-NPs). Owing to the deficiency of studies on the aggregation of TiO2-NPs in the presence of synthetic organic compound,this study used linear alkylbenzene sulfonate( LAS) as a representative to evaluate the effects of TiO2-NPs concentration( 1-10 mg / L),LAS concentration( 0- 1 mg / L),pH( 4- 8)and ionic strength( NaCl,CaCl2; 5- 20 mM) during aggregation of TiO2-NPs suspensions based on the detection of hydrodynamic diameters and electrophoretic mobilities and the calculation of interaction energies.The results showed that the TiO2-NPs in the presence of LAS are more stable than that in the absence of LAS.With the increase of ionic strength,the hydrodynamic diameter of NPs decreases,and the existence of LAS changes the point of zero charge from 5. 4 to a lower value and thus alteres the aggregation behaviors of TiO2-NPs. The present study suggests that the LAS has a significant impact to the transportation and transformation of nanoparticles in aqueous environment.展开更多
Hydrogen with high energy density is an environmental alternative to fossil fuels which can respond to the demand for energy considering environmental conditions.It can be stored on porous materials employing physical...Hydrogen with high energy density is an environmental alternative to fossil fuels which can respond to the demand for energy considering environmental conditions.It can be stored on porous materials employing physical interaction(e.g.adsorption process).The H2storage capacity of materials can be evaluated through electrochemical methods.Therefore,a fast and straightforward approach was employed to fabricate magnesium oxide/chitosan/Au nanoparticles(Mg O/CS/Au)nanocomposites with porous structure for electrochemical hydrogen storage.Herein,laser ablation in water as a fast and green method was utilized to obtain Au nanoparticles(Au NPs).The obtained Au NPs were loaded on Mg O/CS nanocomposite through physical mixing.Structural and morphological investigation of nanocomposites display spherically shaped Au NPs with a diameter of 49–58 nm agglomerated on the Mg O/CS.Drop casting,the fast and cost-effective method was deployed to deposit the benign,and reusable Mg O/CS/Au-x(x is Au NPs weight percentage of 1,3 and 5 wt.%)nanocomposites on stainless steel mesh and their electrochemical hydrogen storage were measured by cyclic voltammetry(CV),indicating good stability and significant hydrogen storage capacity(28 C/g)after 300 CV scans for MgO/CS/Au-1 sample.展开更多
Copper oxide nanoparticles(CuO NPs)were synthesised with laser ablation of a copper sheet immersed in deionized water(DW),cetrimonium bromide(CTAB),and sodium dodecyl sulphate(SDS),respectively.The target was irradiat...Copper oxide nanoparticles(CuO NPs)were synthesised with laser ablation of a copper sheet immersed in deionized water(DW),cetrimonium bromide(CTAB),and sodium dodecyl sulphate(SDS),respectively.The target was irradiated with a pulsed Nd:YAG laser at 1064 nm,600 mJ,a pulse duration of 10 ns,and a repetition rate of 5 Hz.The CuO NPs colloidal were analyzed using UV–Vis spectroscopy,the Fourier transform infrared(FTIR)spectrometer,zeta potential(ZP),X-ray diffraction(XRD),transmission electron microscope(TEM)and field emission scanning electron microscopy(FESEM).The absorption spectra of CuO NPs colloidal showed peaks at 214,215 and 220 nm and low-intensity peaks at 645,650 and 680 nm for SDS,CTAB and DW,respectively.CuO NPs’colloidal results are(−21.6,1.2,and 80 mV)for negatively,neutrally,and positively charged SDS,DW,and CTAB,respectively.The XRD pattern of the NPs revealed the presence of CuO phase planes(110)(111),(20-2)and(11-1).The TEM images revealed nearly spherical NPs,with sizes ranging from 10–90,10–50,and 10–210 nm for CuO NPs mixed with DW,SDS and CTAB,respectively.FESEM images of all the synthesized samples illustrate the formation of spherical nanostructure and large particles are observable.The CuO NPs were tested for antibacterial activity against Streptococcus mutans by using the well diffusion method.In this method,CuO NPs prepared in DW at a concentration of 200μg/mL showed a greater inhibition zone against Streptococcus mutans.展开更多
基金funded by the National Natural Science Foundation of China (21978207 and 21621004)the Natural Science Foundation of Tianjin from Tianjin Municipal Science and Technology Commission (19JCZDJC36800)。
文摘Deposition of β-amyloid protein(Aβ) is the main hallmark of Alzheimer's disease(AD), and it has been well recognized that Cu^(2+)-mediated Aβ aggregation plays a crucial role in AD pathological processes.Cu^(2+)binding to Aβ can promote the production of reactive oxygen species(ROS) through Fenton-like reactions and produce more toxic Aβ-Cu^(2+)species under Cu^(2+)stimulation. Thus, the development of nanomaterials that can inhibit Cu^(2+)-mediated Aβ aggregation and degrade Aβ-Cu^(2+)complexes is considered an effective strategy for the prevention and treatment of AD. In this study, polydopamine nanoparticles(PDA NPs) were prepared and the results reveal that PDA NPs potently inhibit Cu^(2+)-mediated Aβaggregation and effectively reduce the formation of Aβ-Cu^(2+)complexes. In vitro experiments show that PDA NPs efficiently eliminate ROS generation catalyzed by Cu^(2+)or Aβ-Cu^(2+)complexes, thus rescuing cultured cells by reducing intracellular ROS levels. More importantly, PDA NPs can depolymerize Aβ-Cu^(2+)complexes, and the degradation of Aβ-Cu^(2+)complexes is promoted by near-infrared light irradiation due to their high photothermal conversion ability. In vivo studies reveal that PDA NPs significantly reduce the deposition of Aβ plaques in the presence of Cu^(2+)and extend the lifespan of AD nematodes from 11 to 14 d. Thus, the PDA NPs developed herein are multifunctional against Cu^(2+)-mediated Aβ aggregation for the potential prevention and treatment of AD.
文摘This work aims to analyze the flow of electrically conducting MWCNTs-nanofluid over a stretching cylinder with the aggregation and non-aggregation effects of nanoparticles. The working fluid comprised a combination of water and ethylene glycol, with volumetric proportions of (50:50) considered. Convective boundary constraints and modified Fourier law are implemented in heat transmission assessment. The mathematical flow model is formulated in the form of PDEs and is transformed into ODEs via similarity transformation. Numerical outcomes will be obtained with the use of the bvp4c technique and will be displayed with the help of graphs and tables. The results show that the surface drag coefficient is enhanced in the case of aggregation of nanoparticles whereas heat transfer rate is enhanced in the non-aggregation effect of nanoparticles. Furthermore, the temperature distribution enhances the increasing values of particle volume fraction in the case of aggregation effects of nanoparticles whereas temperature distribution lowers in the case of non-aggregation effect of nanoparticles. .
文摘Citrate-reduced silver nanoparticles (Ag-NPs) are used extensively for surface-enhanced Raman scattering (SERS) studies, but are typically found to aggregate using an aggregation agent. This study is aimed at developing a simple, stable, and reproducible aggregated method for Ag-NPs without any aggregation agents in aqueous solutions. The aggregation is induced by the process of centrifugation, water washing and ultrasonication. A mechanism based on the nonuniform distribution of capping ligands is proposed to account for the aggregated structure formation. UV-Vis-NIR extinction spectra and TEM allowed us to identify the existence of Ag-NPs aggregation. Further, due to the polydisperse mixture of Ag-NPs (20~65 nm) used in the present work, Ag-NPs are aggregated closely, which contribute to the observation of low-concentration SERS from the residual citrate layer or even the single-molecule SERS of R6Gon aggregation. After the evaporation of droplet of Ag-NPs aggregation on the Si substrate, citrate or R6Gcould also be detected but with marked redor blue-shifts.
基金financial support from National Research Foundation Investigatorship (R279-000-444-281)National University of Singapore (R279-000-482-133)
文摘Photodynamic therapy(PDT) employs accumulation of photosensitizers(PSs) in malignant tumor tissue followed by the light-induced generation of cytotoxic reactive oxygen species to kill the tumor cells. The success of PDT depends on optimal PS dosage that is matched with the ideal power of light. This in turn depends on PS accumulation in target tissue and light administration time and period.As theranostic nanomedicine is driven by multifunctional therapeutics that aim to achieve targeted tissue delivery and image-guided therapy, fluorescent PS nanoparticle(NP)accumulation in target tissues can be ascertained through fluorescence imaging to optimize the light dose and administration parameters. In this regard, zebrafish larvae provide a unique transparent in vivo platform to monitor fluorescent PS bio-distribution and their therapeutic efficiency. Using fluorescent PS NPs with unique aggregation-induced emission characteristics, we demonstrate for the first time the real-time visualization of polymeric NP accumulation in tumor tissue and, more importantly, the best time to conduct PDT using transgenic zebrafish larvae with inducible liver hyperplasia as an example.
文摘A sensitivity analysis is performed to analyze the effects of the nanoparticle(NP)aggregation and thermal radiation on heat transport of the nanoliquids(titania based on ethylene glycol)over a vertical cylinder.The optimization of heat transfer rate and friction factor is performed for NP volume fraction(1%≤φ≤3%),radiation parameter(1≤R_(t)≤3),and mixed convection parameter(1.5≤λ≤2.5)via the facecentered central composite design(CCD)and the response surface methodology(RSM).The modified Krieger and Dougherty model(MKDM)for dynamic viscosity and the Bruggeman model(BM)for thermal conductivity are utilized to simulate nanoliquids with the NP aggregation aspect.The complicated nonlinear problem is treated numerically.It is found that the temperature of nanoliquid is enhanced due to the aggregation of NPs.The friction factor is more sensitive to the volume fraction of NPs than the thermal radiation and the mixed convection parameter.Furthermore,the heat transport rate is more sensitive to the effect of radiative heat compared with the NP volume fraction and mixed convection parameter.
基金supported by Natural Science Foundation of China(Project No.41206035,41602231)The National Nature Science Foundation of Guangdong Province(Project No.2015A030313157)
文摘1 Introduction Nanoparticles are widely found in the ductile shear zone and it is considered to have a close relation with faulting.The sizes of these nanoparticles are generallyless than 100 nm.They have a variety of morphologies like globular structure rod-like and tubular,by the order aggregating of these nanoparticles various aggregations
基金Project supported by the Natural Science Foundation of Fujian Province of China(Grant No.2012J05008)
文摘We present the results of molecular dynamics simulations of net positively charged fullerene nanoparticles in salt- free and salt-added solution. The aggregation of fullerene (C60)-like nanoparticle and counterion are studied in detail as a function of temperatures and a finite salt concentration. Our simulations show that the strong conformation changes as temperature changes. The net positively-charged nanoparticles do not repel each other but are condensed under proper temperatures. If salts are added, the aggregated nanoparticles will be disaggregated due to the Debye screening effect.
文摘The current exploration focuses on the ethylene glycol(EG)based nanoliquid flow in a microchannel.The effectiveness of the internal heat source and linear radiation is reflected in the present investigation.The estimation of suitable thermal conductivity model has affirmative impact on the convective heat transfer phenomenon.The examination is conceded with the nanoparticle aggregation demonstrated by the Maxwell-Bruggeman and Krieger-Dougherty models which tackle the formation of nanolayer.These models effectively describe the thermal conductivity and viscosity correspondingly.The dimensionless mathematical expressions are solved numerically by the Runge Kutta Fehlberg approach.A higher thermal field is attained for the Bruggeman model due to the formation of thermal bridge.A second law analysis is carried out to predict the sources of irreversibility associated with the thermal system.It is remarked that lesser entropy generation is obtained for the aggregation model.The entropy generation rate declines with the slip flow and the thermal heat flux.A notable enhancement in the Bejan number is attained by increasing the Biot number.It is established that the nanoparticle aggragation model exhibits a higher Bejan number in comparision with the usual flow model.
文摘Melanin, the main photoreceptor in living organics, is largely abundant in pigmented hair tissues. Its promising properties have been widely studied in order to fully explore the corresponding applications. However, the photodegradating and aggregating natures of melanin, to some extent, restrict the development of this eco-friendly biomaterial. This study is conducted to overcome both disadvantages by a mature method that coating the original melanin nanoparticles with silica as shells. The results revealed that the aggregation of the melanin/silica nanoparticles (MSNP) was decreased obviously by a 5 h coating and appeared a uniformly mono-dispersed solution. The MSNP that coated for 20 h provided an efficient protection on the photodegradation of the melanin with a 50% maintenance of the melanin content compared with 16% of original melanin and 2% of MSNP-5 h.
基金Sponsered by the State Key Laboratory of Urban Water Resource and Environment(Grant No.2014DX06)the Natural Science Foundation of Heilongjiang Province of China(Grant No.E201154)the Natural Science Foundation of China(Grant No.51208142)
文摘In aqueous environment,organic matters may exert impacts on the aggregation behaviors of titanium dioxide nanoparticles( TiO2-NPs). Owing to the deficiency of studies on the aggregation of TiO2-NPs in the presence of synthetic organic compound,this study used linear alkylbenzene sulfonate( LAS) as a representative to evaluate the effects of TiO2-NPs concentration( 1-10 mg / L),LAS concentration( 0- 1 mg / L),pH( 4- 8)and ionic strength( NaCl,CaCl2; 5- 20 mM) during aggregation of TiO2-NPs suspensions based on the detection of hydrodynamic diameters and electrophoretic mobilities and the calculation of interaction energies.The results showed that the TiO2-NPs in the presence of LAS are more stable than that in the absence of LAS.With the increase of ionic strength,the hydrodynamic diameter of NPs decreases,and the existence of LAS changes the point of zero charge from 5. 4 to a lower value and thus alteres the aggregation behaviors of TiO2-NPs. The present study suggests that the LAS has a significant impact to the transportation and transformation of nanoparticles in aqueous environment.
文摘Hydrogen with high energy density is an environmental alternative to fossil fuels which can respond to the demand for energy considering environmental conditions.It can be stored on porous materials employing physical interaction(e.g.adsorption process).The H2storage capacity of materials can be evaluated through electrochemical methods.Therefore,a fast and straightforward approach was employed to fabricate magnesium oxide/chitosan/Au nanoparticles(Mg O/CS/Au)nanocomposites with porous structure for electrochemical hydrogen storage.Herein,laser ablation in water as a fast and green method was utilized to obtain Au nanoparticles(Au NPs).The obtained Au NPs were loaded on Mg O/CS nanocomposite through physical mixing.Structural and morphological investigation of nanocomposites display spherically shaped Au NPs with a diameter of 49–58 nm agglomerated on the Mg O/CS.Drop casting,the fast and cost-effective method was deployed to deposit the benign,and reusable Mg O/CS/Au-x(x is Au NPs weight percentage of 1,3 and 5 wt.%)nanocomposites on stainless steel mesh and their electrochemical hydrogen storage were measured by cyclic voltammetry(CV),indicating good stability and significant hydrogen storage capacity(28 C/g)after 300 CV scans for MgO/CS/Au-1 sample.
文摘Copper oxide nanoparticles(CuO NPs)were synthesised with laser ablation of a copper sheet immersed in deionized water(DW),cetrimonium bromide(CTAB),and sodium dodecyl sulphate(SDS),respectively.The target was irradiated with a pulsed Nd:YAG laser at 1064 nm,600 mJ,a pulse duration of 10 ns,and a repetition rate of 5 Hz.The CuO NPs colloidal were analyzed using UV–Vis spectroscopy,the Fourier transform infrared(FTIR)spectrometer,zeta potential(ZP),X-ray diffraction(XRD),transmission electron microscope(TEM)and field emission scanning electron microscopy(FESEM).The absorption spectra of CuO NPs colloidal showed peaks at 214,215 and 220 nm and low-intensity peaks at 645,650 and 680 nm for SDS,CTAB and DW,respectively.CuO NPs’colloidal results are(−21.6,1.2,and 80 mV)for negatively,neutrally,and positively charged SDS,DW,and CTAB,respectively.The XRD pattern of the NPs revealed the presence of CuO phase planes(110)(111),(20-2)and(11-1).The TEM images revealed nearly spherical NPs,with sizes ranging from 10–90,10–50,and 10–210 nm for CuO NPs mixed with DW,SDS and CTAB,respectively.FESEM images of all the synthesized samples illustrate the formation of spherical nanostructure and large particles are observable.The CuO NPs were tested for antibacterial activity against Streptococcus mutans by using the well diffusion method.In this method,CuO NPs prepared in DW at a concentration of 200μg/mL showed a greater inhibition zone against Streptococcus mutans.